This study presents an innovative urea detection method utilizing pH-controlled Fenton etching of gold nanobipyramids(AuNBPs),offering a multicolor visual response.By leveraging the urease-catalyzed hydrolysis of urea...This study presents an innovative urea detection method utilizing pH-controlled Fenton etching of gold nanobipyramids(AuNBPs),offering a multicolor visual response.By leveraging the urease-catalyzed hydrolysis of urea,which releases ammonia and raises pH,the Fenton reaction is inhibited,reducing the etching of AuNBPs.This approach enables a highly sensitive and distinct multichromatic response across a wide range of urea concentrations,particularly at low target levels.The solution-based sensor achieved an exceptionally low detection limit of 0.098μM,surpassing existing colorimetric urea biosensors.Furthermore,embedding the sensor in an agarose hydrogel matrix to create a solid-state format resulted in a detection limit of 0.2μM.Real-world validation demonstrated high recovery rates in urine samples,further affirming the sensor’s reliability.This multicolor biosensing platform offers a robust tool for point-of-care diagnostics,facilitating accurate and user-friendly urea detection.展开更多
基金supported by the Institute for Information&Communications Technology Planning&Evaluation(IITP)grant funded by the Korea government(MSIT)(RS-2023-00228994),RS-2024-00346003the National Research Foundation of Korea(2020R1A5A1018052)and(RS-2024-00410209).
文摘This study presents an innovative urea detection method utilizing pH-controlled Fenton etching of gold nanobipyramids(AuNBPs),offering a multicolor visual response.By leveraging the urease-catalyzed hydrolysis of urea,which releases ammonia and raises pH,the Fenton reaction is inhibited,reducing the etching of AuNBPs.This approach enables a highly sensitive and distinct multichromatic response across a wide range of urea concentrations,particularly at low target levels.The solution-based sensor achieved an exceptionally low detection limit of 0.098μM,surpassing existing colorimetric urea biosensors.Furthermore,embedding the sensor in an agarose hydrogel matrix to create a solid-state format resulted in a detection limit of 0.2μM.Real-world validation demonstrated high recovery rates in urine samples,further affirming the sensor’s reliability.This multicolor biosensing platform offers a robust tool for point-of-care diagnostics,facilitating accurate and user-friendly urea detection.
