First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provi...First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.展开更多
Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different bakin...Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different baking temperatures, baking time, humidity, open space time, sample morphology and other conditions. The contents of Atractylodes macrocephala Koidz I, II and III were determined by HPLC, and the change rules were analyzed. Results: for the original medicinal materials and decoction pieces, temperature and heating time had a certain effect on the conversion amount, but the effect was limited. However, under the influence of the appropriate heating temperature and heating time, the content of pulverized sample powder changed significantly. In the case of Atractylodes macrocephala Koidz in the form of powder, with the increase of storage time, the content of Atractylenolide I, II and III changed significantly. Conclusion: comparison of the content change law of Atractylodes macrocephala Koidz lactone I, II, III is conducive to optimizing the processing method of Atractylodes macrocephala Koidz, and provides a reference for the quality control of Atractylodes macrocephala Koidz.展开更多
Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contain...Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contains volatile oil,atractylenolides,atractylodes polysaccharides,glycosides and amino acids.And it has the medical functions of good for spleen and intestine,diuretic and dehumidification,hidroschesis,miscarriage prevention and soon.In order to provide references for further development and utilization,this paper systematic arranged the Chinese medicine atractylodes chemical composition,pharmacological effects,processing technology and effect of processing technology on chemical composition and pharmacological action.展开更多
We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced o...We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced on Murashige-Skoog(MS)medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ),6-benzylaminopurine(BA)andα-naphthaleneacetic acid(NAA).Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid(IBA).The maximum mean number of shoots(5.61)was obtained from a single explant by the combined effect of 1.08μmol/L NAA and 2.25μmol/L TDZ.The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators.The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7μmol/L NAA.展开更多
Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, trad...Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.展开更多
Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for stren...Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.展开更多
文摘First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.
文摘Objective: to study the effect of different conditions on the content change and internal law of Atractylenolide I, II and III. Methods: the samples of Atractylodes macrocephala Koidz were treated with different baking temperatures, baking time, humidity, open space time, sample morphology and other conditions. The contents of Atractylodes macrocephala Koidz I, II and III were determined by HPLC, and the change rules were analyzed. Results: for the original medicinal materials and decoction pieces, temperature and heating time had a certain effect on the conversion amount, but the effect was limited. However, under the influence of the appropriate heating temperature and heating time, the content of pulverized sample powder changed significantly. In the case of Atractylodes macrocephala Koidz in the form of powder, with the increase of storage time, the content of Atractylenolide I, II and III changed significantly. Conclusion: comparison of the content change law of Atractylodes macrocephala Koidz lactone I, II, III is conducive to optimizing the processing method of Atractylodes macrocephala Koidz, and provides a reference for the quality control of Atractylodes macrocephala Koidz.
基金supported by National Natural Science Foundation of China(81473549)National Natural Science Foundation of China Youth Fund Project(31402237)+1 种基金National "Major New Drug Creation" Major Science and Technology Projects(2014ZX09304-306-04)Ministry of Education of the Central University Basic Scientific Research Business Fee(XDJK2015D016)
文摘Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contains volatile oil,atractylenolides,atractylodes polysaccharides,glycosides and amino acids.And it has the medical functions of good for spleen and intestine,diuretic and dehumidification,hidroschesis,miscarriage prevention and soon.In order to provide references for further development and utilization,this paper systematic arranged the Chinese medicine atractylodes chemical composition,pharmacological effects,processing technology and effect of processing technology on chemical composition and pharmacological action.
基金This work was partially funded by the Science and Technology Department of Zhejiang Province,China(Nos.2005C13016 and 2005C22080).
文摘We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced on Murashige-Skoog(MS)medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ),6-benzylaminopurine(BA)andα-naphthaleneacetic acid(NAA).Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid(IBA).The maximum mean number of shoots(5.61)was obtained from a single explant by the combined effect of 1.08μmol/L NAA and 2.25μmol/L TDZ.The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators.The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7μmol/L NAA.
基金the National Natural Science Foundation of China(81973221)National Natural Science Foundation for Young Scientists of China(81603647)+2 种基金the Women and Children Health Talent Project of Jiangsu Province(FRC201785)the Chinese Clinical Medicine Innovation Center of Obstetrics,Gynecology,and Reproduction in Jiangsu Province(ZX202102)the Women and Children Health Science Foundation of Jiangsu Province(F202206).
文摘Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.
基金funded by the Jiangxi Provincial Natural Science Foundation Youth Project(20212BAB216011)the Second National Famous ChineseMedicine Practitioner(Gong Qianfeng)Inheritance Workshop of the State Administration of Traditional ChineseMedicine(State Chinese Medicine Office Human Education Letter[2022]no.245)+2 种基金2023 National Chinese Medicine Characteristic Technology Inheritance Talent Training Program(State Chinese Medicine Human Education Letter[2023]no.96)Jiangxi Province,the young and medium-aged backbone talents of traditional Chinese medicine(the fourth batch)(Gan TCM Science and Education[2022]no.7)the seventh batch of the national old Chinese medicine experts in academic experience successors(State Chinesemedicine human education letter[2022]no.76).
文摘Background:Currently,traditional methods of treating acute gastric ulcer(AGU)have many drawbacks,necessitating an alternative therapy with fewer adverse effects.Atractylodis Macrocephalae Rhizoma(BZ)is known for strengthening the spleen and harmonizing the stomach.BZ processed with Aurantii Fructus Immaturus juice(ZSZBZ),a classic decoction since the Han Dynasty,can enhance the efficacy of BZ.However,the key active components and targets of action of ZSZBZ remain undiscovered.Aimof the study:This study aimed to investigate the bioactive chemical constituents of ZSZBZ against AGU and their possible mechanisms of action,elucidating the scientific content of ZSZBZ processing.Materials and methods:Initially,we examined rat stomach histopathology and conducted ELISA for oxidative stress and inflammation.Subsequently,we investigated underlying mechanisms using metabolomics.Further analysis of potent components and key targets in ZSZBZ was conducted through liquid chromatography-mass spectrometry analysis combined with network pharmacology.Finally,key targets were analyzed by Western blot.Results:ZSZBZ improved gastric histopathology,reversing high alcohol-induced oxidative stress(SOD,CAT)and inflammatory level(TNF-α,IL-6)disorders.This is associated with ZSZBZ’s regulation of amino acid metabolism,energy metabolism,and inflammatory response-related metabolic pathways,along with key targets PTGS2,MAPK1,and KDR.The significant increase in potency of ZSZBZ may be attributed to elevated levels of naringenin,hesperidin,hesperidin,and rhamnoceroside after concoction.Conclusions:Combining metabolomics and network pharmacology,this study elucidated that ZSZBZ enhanced gastroprotection by modulating amino acid metabolism,antioxidant,and inflammation-related targets and pathways,providing insights into the bioactive compounds and potential mechanisms of herbal concoctions.
