Aspartate/asparagine-β-hydroxylase(ASPH),a type Ⅱ transmembraneα-ketoglutarate-dependent hydroxylase,is frequently overexpressed in various cancers.However,its role in lung adenocarcinoma(LUAD)remains unclear.Here,...Aspartate/asparagine-β-hydroxylase(ASPH),a type Ⅱ transmembraneα-ketoglutarate-dependent hydroxylase,is frequently overexpressed in various cancers.However,its role in lung adenocarcinoma(LUAD)remains unclear.Here,we analyzed ASPH expression in LUAD and normal lung tissues using bioinformatic tools,and evaluated its association with clinicopathological characteristics and patient prognosis.High ASPH expression correlated with advanced disease and poor survival,and served as an independent prognostic factor.Functional assays,including Transwell,scratch healing,and murine lung metastasis models,demonstrated that ASPH promotes LUAD cell migration and metastasis.The potential molecular mechanisms by which ASPH exerts its function in LUAD were explored by transcriptome sequencing analysis and immunoprecipitation combined with mass spectrometry(IP-MS).Further analysis showed that ASPH enhances activation of the MAPK and Notch signaling pathways through RUVBL2 interaction.Collectively,our findings suggest that ASPH contributes to LUAD progression by engaging RUVBL2 and activating key oncogenic signaling cascades,highlighting its potential as a prognostic biomarker and therapeutic target.展开更多
The family of cubic polyoxopalladates encapsulating lanthanide ions[LnPd_(12)(AsPh)_(8)O_(32)]^(5−)where Ln=Tb,Dy,Ho,Er and Tm,is magnetically characterised and theoretically described by the Radial Effective Charge(R...The family of cubic polyoxopalladates encapsulating lanthanide ions[LnPd_(12)(AsPh)_(8)O_(32)]^(5−)where Ln=Tb,Dy,Ho,Er and Tm,is magnetically characterised and theoretically described by the Radial Effective Charge(REC)model and a phenomenological crystal-field approach using the full-hamiltonian,in the SIMPRE and CONDON packages respectively.The lack of anisotropy generates an extraordinarily rich energy level structure at low temperatures,which allows us to study how such a structure is affected by lifting the strict cubic symmetry and/or by applying an external magnetic field.In particular,we will explore the possibility of using these cubic Ln complexes as spin-qubits.We will focus on the Ho derivative.We find that it is possible to reach a regime where decoherence caused by the nuclear spin bath is quenched for moderate axial compression of the cube and small magnetic fields.展开更多
基金supported in part by the grant from Guangdong Basic and Applied Basic Research Fund(2023A1515110995).
文摘Aspartate/asparagine-β-hydroxylase(ASPH),a type Ⅱ transmembraneα-ketoglutarate-dependent hydroxylase,is frequently overexpressed in various cancers.However,its role in lung adenocarcinoma(LUAD)remains unclear.Here,we analyzed ASPH expression in LUAD and normal lung tissues using bioinformatic tools,and evaluated its association with clinicopathological characteristics and patient prognosis.High ASPH expression correlated with advanced disease and poor survival,and served as an independent prognostic factor.Functional assays,including Transwell,scratch healing,and murine lung metastasis models,demonstrated that ASPH promotes LUAD cell migration and metastasis.The potential molecular mechanisms by which ASPH exerts its function in LUAD were explored by transcriptome sequencing analysis and immunoprecipitation combined with mass spectrometry(IP-MS).Further analysis showed that ASPH enhances activation of the MAPK and Notch signaling pathways through RUVBL2 interaction.Collectively,our findings suggest that ASPH contributes to LUAD progression by engaging RUVBL2 and activating key oncogenic signaling cascades,highlighting its potential as a prognostic biomarker and therapeutic target.
基金funded by the EU(ERC Advanced Grant SPINMOL and ERC Consolidator Grant DECRESIM),the Spanish MINECO(grant MAT2014-56143-R,CTQ2014-52758-P)the Generalitat Valenciana(Prometeo and ISIC Programmes of excellence).A.G.-A.acknowledges funding by the MINECO(Ramón y Cajal contract).E.C.and J.J.B.acknowledge the Blaise Pascal International Chair for financial support+1 种基金J.J.B.also thanks the Spanish MECD for an FPU predoctoral grant.U.K.thanks the German Science Foundation(DFG KO-2288/20-1 and KO-2288/8-1)Jacobs University for research support,and also acknowledges the COST Actions CM1006(EUFEN)and CM1203(PoCheMoN).P.Y.thanks the CSC(China Scholarship Council)for a doctoral fellowship.
文摘The family of cubic polyoxopalladates encapsulating lanthanide ions[LnPd_(12)(AsPh)_(8)O_(32)]^(5−)where Ln=Tb,Dy,Ho,Er and Tm,is magnetically characterised and theoretically described by the Radial Effective Charge(REC)model and a phenomenological crystal-field approach using the full-hamiltonian,in the SIMPRE and CONDON packages respectively.The lack of anisotropy generates an extraordinarily rich energy level structure at low temperatures,which allows us to study how such a structure is affected by lifting the strict cubic symmetry and/or by applying an external magnetic field.In particular,we will explore the possibility of using these cubic Ln complexes as spin-qubits.We will focus on the Ho derivative.We find that it is possible to reach a regime where decoherence caused by the nuclear spin bath is quenched for moderate axial compression of the cube and small magnetic fields.
