Medical hemostatic gauze is one of the most common agents for bleeding management used in pre-hospital care and clinical treatment.An ideal hemostat requires the features including fast coagulation ability,high biocom...Medical hemostatic gauze is one of the most common agents for bleeding management used in pre-hospital care and clinical treatment.An ideal hemostat requires the features including fast coagulation ability,high biocompatibility and low cost,which is difficult to be achieved simultaneously.Herein,we reported a chemical immobilization method to uniformly anchor the zeolitic imidazolate framework(ZIF-8)nanoparticles on polyvinyl alcohol(PVA)membrane,which dramatically accelerated the in vivo conversion process of prothrombin to thrombin,achieving a short hemostasis time around 60 s with a low amount of blood loss of 23 mg.Later,the hemostatic mechanism was unveiled by two pathways involving the activation of platelets and the conversion of prothrombin,indicating that this ZIF-8-based membrane works in a similar way to natural platelet-based physiological processes.More importantly,the convenient manufacturing and excellent biocompatibility of ZIF-8-based membrane provide a practical candidate hemostat for clinical bleeding management.展开更多
Abstract:Objective To investigate whether artificial heterozygous chimeras of platelets can be established by intrauterine transplantation of fetal liver stem cells and evaluate its potential use for the treatment of ...Abstract:Objective To investigate whether artificial heterozygous chimeras of platelets can be established by intrauterine transplantation of fetal liver stem cells and evaluate its potential use for the treatment of Glanzmann thrombasthenia.Methods Platelet glycoprotein (GP) Ⅱb Bak a/b (or GPⅡb Ⅰle843Ser) was used as a genetic marker. A homozygous 16-week-old Bak a/a fetus (as donor) and a homozygous 16.5-week-old Bak b/b fetus (as recipient) were screened from 42 pregnant women hospitalized for abortion. PCR with allele specific primers and FOK Ⅰ digestion based on PCR products were used. Aborted donor fetal liver cell suspensions were prepared and intrauterine transplantation was carried out by infusion of 4?ml fetal liver cells (22×105) into the recipient umbilical vein under ultrasonic visualization.Results At gestation termination (abortion), 21 days after transplantation, chimera GPⅡb Bak a/b of the recipient were detected by FOK 1 digestion based on PCR from DNA and RT-PCR from platelet RNA. Conclusion Intrauterine transplantation of fetal liver cell may provide an effective way for curing GT or other inherited diseases.展开更多
基金supported by the National Natural Science Foundation of China(U23A2070 and 22201223)Chutian Scholar Foundation of Hubei Province and Natural Science Foundation of Hubei Province,China(2022CFA023).
文摘Medical hemostatic gauze is one of the most common agents for bleeding management used in pre-hospital care and clinical treatment.An ideal hemostat requires the features including fast coagulation ability,high biocompatibility and low cost,which is difficult to be achieved simultaneously.Herein,we reported a chemical immobilization method to uniformly anchor the zeolitic imidazolate framework(ZIF-8)nanoparticles on polyvinyl alcohol(PVA)membrane,which dramatically accelerated the in vivo conversion process of prothrombin to thrombin,achieving a short hemostasis time around 60 s with a low amount of blood loss of 23 mg.Later,the hemostatic mechanism was unveiled by two pathways involving the activation of platelets and the conversion of prothrombin,indicating that this ZIF-8-based membrane works in a similar way to natural platelet-based physiological processes.More importantly,the convenient manufacturing and excellent biocompatibility of ZIF-8-based membrane provide a practical candidate hemostat for clinical bleeding management.
基金ThisworkwassupportedbyNaturalScienceFoundationofHunan Province (No 1995 2 5 2 3)
文摘Abstract:Objective To investigate whether artificial heterozygous chimeras of platelets can be established by intrauterine transplantation of fetal liver stem cells and evaluate its potential use for the treatment of Glanzmann thrombasthenia.Methods Platelet glycoprotein (GP) Ⅱb Bak a/b (or GPⅡb Ⅰle843Ser) was used as a genetic marker. A homozygous 16-week-old Bak a/a fetus (as donor) and a homozygous 16.5-week-old Bak b/b fetus (as recipient) were screened from 42 pregnant women hospitalized for abortion. PCR with allele specific primers and FOK Ⅰ digestion based on PCR products were used. Aborted donor fetal liver cell suspensions were prepared and intrauterine transplantation was carried out by infusion of 4?ml fetal liver cells (22×105) into the recipient umbilical vein under ultrasonic visualization.Results At gestation termination (abortion), 21 days after transplantation, chimera GPⅡb Bak a/b of the recipient were detected by FOK 1 digestion based on PCR from DNA and RT-PCR from platelet RNA. Conclusion Intrauterine transplantation of fetal liver cell may provide an effective way for curing GT or other inherited diseases.
