Silicon-based neural microneedle arrays,such as the Utah Array,have demonstrated excellent performance in chronic recordings from the cerebral cortex.Unlike planar thin-film electrodes with recording sites arranged on...Silicon-based neural microneedle arrays,such as the Utah Array,have demonstrated excellent performance in chronic recordings from the cerebral cortex.Unlike planar thin-film electrodes with recording sites arranged on the surface of a silicon film,the recording sites of microneedle arrays are located at the tips of three-dimensional needles,which significantly complicates the fabrication process required for single-neuron recordings.To address this challenge,we develop a local de-insulation method for microneedle recording electrodes that eliminates the need for etching:the microneedle tips are encapsulated in a controllable-thickness protective layer,followed by deposition of a Parylene-C insulation layer.By optimizing the elasticity of the protection material,as well as its adhesion and shape on both the protective layer and the electrode shaft,we were able to precisely control the area of the removed insulated layers,resulting in consistent tip exposure.Experimental results show that the non-uniformity of the exposed microneedle recording sites in the silicon-based neural microelectrode arrays(each has 10×10 array)fabricated using this method is 3.32±1.02%.Furthermore,the arrays exhibited high stability and reliability in both mechanical performance and electrical characteristics.They achieved an average spike signal-to-noise ratio of 12.63±6.64 during in vivo testing.This fabrication technique provides a valuable method for the development of high-performance neural microelectrode array.展开更多
基金supported by the National Natural Science Foundation of China under grant 62071447the National Key R&D Program of China under grant 2022YFF1202303.
文摘Silicon-based neural microneedle arrays,such as the Utah Array,have demonstrated excellent performance in chronic recordings from the cerebral cortex.Unlike planar thin-film electrodes with recording sites arranged on the surface of a silicon film,the recording sites of microneedle arrays are located at the tips of three-dimensional needles,which significantly complicates the fabrication process required for single-neuron recordings.To address this challenge,we develop a local de-insulation method for microneedle recording electrodes that eliminates the need for etching:the microneedle tips are encapsulated in a controllable-thickness protective layer,followed by deposition of a Parylene-C insulation layer.By optimizing the elasticity of the protection material,as well as its adhesion and shape on both the protective layer and the electrode shaft,we were able to precisely control the area of the removed insulated layers,resulting in consistent tip exposure.Experimental results show that the non-uniformity of the exposed microneedle recording sites in the silicon-based neural microelectrode arrays(each has 10×10 array)fabricated using this method is 3.32±1.02%.Furthermore,the arrays exhibited high stability and reliability in both mechanical performance and electrical characteristics.They achieved an average spike signal-to-noise ratio of 12.63±6.64 during in vivo testing.This fabrication technique provides a valuable method for the development of high-performance neural microelectrode array.
