The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy(SMLM)by overcoming the limit imposed by the fluorophore's photon counts.Typical MINFLUX microscopes...The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy(SMLM)by overcoming the limit imposed by the fluorophore's photon counts.Typical MINFLUX microscopes localize the target molecule by scanning a zero-intensity focus around the molecule in a circular trajectory,with smaller trajectory diameters yielding better localization uncertainties for a given number of photons.Since this approach requires the molecule to be within the scanned trajectory,MINFLUX typically relies on an iterative scheme with decreasing trajectory diameters.This iterative approach is prone to misplacements of the trajectory and increases the system's complexity.In this work,we introduce ISM-FLUX,a novel implementation of MINFLUX using image-scanning microscopy(ISM)with a single-photon avalanche diode array detector.ISM-FLUX provides a precise MINFLUX localization within the trajectory while maintaining a conventional photon-limited uncertainty outside it.The robustness of ISM-FLUX localization results in a larger localization range and greatly simplifies the architecture,which may facilitate broader adoption of MINFLUX.展开更多
In order to improve the infrared detection and discrimination ability of the smart munition to the dynamic armor target under the complex background,the multi-line array infrared detection system is established based ...In order to improve the infrared detection and discrimination ability of the smart munition to the dynamic armor target under the complex background,the multi-line array infrared detection system is established based on the combination of the single unit infrared detector.The surface dimension features of ground armored targets are identified by size calculating solution algorithm.The signal response value and the value of size calculating are identified by the method of fuzzy recognition to make the fuzzy classification judgment for armored target.According to the characteristics of the target signal,a custom threshold de-noising function is proposed to solve the problem of signal preprocessing.The multi-line array infrared detection can complete the scanning detection in a large area in a short time with the characteristics of smart munition in the steady-state scanning stage.The method solves the disadvantages of wide scanning interval and low detection probability of single unit infrared detection.By reducing the scanning interval,the number of random rendezvous in the infrared feature area of the upper surface is increased,the accuracy of the size calculating is guaranteed.The experiments results show that in the fuzzy recognition method,the size calculating is introduced as the feature operator,which can improve the recognition ability of the ground armor target with different shape size.展开更多
To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperativ...To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperative tracking control method considering the effectiveness of passive detection. First, the system model of passive detection in UAV formation is constructed.Then, the Geometric Dilution of Precision(GDOP) of bearing-only sensor nodes pair on the observation plane is analyzed. Building on this foundation, the pairwise form is expanded to obtain the optimal geometric configuration for the entire network. Subsequently, the Distributed Cubature Information Filtering(DCIF) is integrated with the weighted average consensus protocol to design the distributed cooperative observer suitable for the system model, enabling state estimation of the target. Finally, within the distributed architecture, the Nonlinear Model Predictive Controller(NMPC) is designed. This controller autonomously assembles the UAV formation during the assembly phase and forms an optimal detection array. The UAV formation then tracks the target using the virtual geometric center based on the established rigid geometric configuration. The simulation experiments validate that the proposed model and method can enhance the passive detection effectiveness of the UAV formation, thereby achieving stable and efficient distributed cooperative tracking for the maneuvering target.展开更多
Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.M...Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.Methods展开更多
The special seismic tectonic environment and frequent seismicity in the southeastern margin of the Qinghai-Tibet Plateau show that this area is an ideal location to study the present tectonic movement and background o...The special seismic tectonic environment and frequent seismicity in the southeastern margin of the Qinghai-Tibet Plateau show that this area is an ideal location to study the present tectonic movement and background of strong earthquakes in China's Mainland and to predict future strong earthquake risk zones.Studies of the structural environment and physical characteristics of the deep structure in this area are helpful to explore deep dynamic effects and deformation field characteristics,to strengthen our understanding of the roles of anisotropy and tectonic deformation and to study the deep tectonic background of the seismic origin of the block's interior.In this paper,the three-dimensional(3D)P-wave velocity structure of the crust and upper mantle under the southeastern margin of the Qinghai-Tibet Plateau is obtained via observational data from 224 permanent seismic stations in the regional digital seismic network of Yunnan and Sichuan Provinces and from 356 mobile China seismic arrays in the southern section of the north-south seismic belt using a joint inversion method of the regional earthquake and teleseismic data.The results indicate that the spatial distribution of the P-wave velocity anomalies in the shallow upper crust is closely related to the surface geological structure,terrain and lithology.Baoxing and Kangding,with their basic volcanic rocks and volcanic clastic rocks,present obvious high-velocity anomalies.The Chengdu Basin shows low-velocity anomalies associated with the Quaternary sediments.The Xichang Mesozoic Basin and the Butuo Basin are characterised by low-velocity anomalies related to very thick sedimentary layers.The upper and middle crust beneath the Chuan-Dian and Songpan-Ganzi Blocks has apparent lateral heterogeneities,including low-velocity zones of different sizes.There is a large range of low-velocity layers in the Songpan-Ganzi Block and the sub-block northwest of Sichuan Province,showing that the middle and lower crust is relatively weak.The Sichuan Basin,which is located in the western margin of the Yangtze platform,shows high-velocity characteristics.The results also reveal that there are continuous low-velocity layer distributions in the middle and lower crust of the Daliangshan Block and that the distribution direction of the low-velocity anomaly is nearly SN,which is consistent with the trend of the Daliangshan fault.The existence of the low-velocity layer in the crust also provides a deep source for the deep dynamic deformation and seismic activity of the Daliangshan Block and its boundary faults.The results of the 3D P-wave velocity structure show that an anomalous distribution of high-density,strong-magnetic and high-wave velocity exists inside the crust in the Panxi region.This is likely related to late Paleozoic mantle plume activity that led to a large number of mafic and ultra-mafic intrusions into the crust.In the crustal doming process,the massive intrusion of mantle-derived material enhanced the mechanical strength of the crustal medium.The P-wave velocity structure also revealed that the upper mantle contains a low-velocity layer at a depth of 80-120 km in the Panxi region.The existence of deep faults in the Panxi region,which provide conditions for transporting mantle thermal material into the crust,is the deep tectonic background for the area's strong earthquake activity.展开更多
A novel method for the determination of five carbamate pesticides （metolcarb, carbofuran, carbaryl, isoprocard and diethofencard） in water samples was developed by dispersive liquid-liquid microextraction （DLLME） ...A novel method for the determination of five carbamate pesticides （metolcarb, carbofuran, carbaryl, isoprocard and diethofencard） in water samples was developed by dispersive liquid-liquid microextraction （DLLME） coupled with high performance liquid chromatography-diode array detector （HPLC-DAD）. Some experimental parameters that influence the extraction efficiency were studied and optimized to obtain the best extraction results. Under the optimum conditions for the method, the calibration curve was linear in the concentration range from 5 to 1000 ng mL^-1 for all the five carbamate pesticides, with the correlation coefficients （r^2） varying from 0.9984 to 0.9994. Good enrichment factors were achieved ranging from 80 to 177- fold, depending on the compound. The limits of detection （LODs） （S/N = 3） were ranged from 0.1 to 0.5 ng mL^-1. The method has been successfully applied to the analysis of the pesticide residues in environmental water samples.展开更多
The dynamic parameters of multiple projectiles that are fired using multi-barrel weapons in highfrequency continuous firing modes are important indicators to measure the performance of these weapons.The characteristic...The dynamic parameters of multiple projectiles that are fired using multi-barrel weapons in highfrequency continuous firing modes are important indicators to measure the performance of these weapons.The characteristics of multiple projectiles are high randomness and large numbers launched in a short period of time,making it very difficult to obtain the real dispersion parameters of the projectiles due to the occlusion or coincidence of multiple projectiles.Using six intersecting-screen testing system,in this paper,we propose an association recognition and matching algorithm of multiple projectiles using a temporal and spatial information constraint mechanism.We extract the output signal from each detection screen and then use the wavelet transform to process the output signal.We present a method to identify and extract the time values on which the projectiles pass through the detection screens using the wavelet transform modulus maximum theory.We then use the correlation of the output signals of three parallel detection screens to establish a correlation coefficient recognition constraint function for the multiple projectiles.Based on the premise of linear projectile motion,we establish a temporal and spatial constraint matching model using the projectile’s position coordinates in each detection screen and the projectile’s time constraints within the multiple intersecting-screen geometry.We then determine the time values of the multiple projectiles in each detection screen using an iterative search cycle registration,and finally obtain the flight parameters for the multiple projectiles in the presence of uncertainty.The proposed method and algorithm were verified experimentally and can solve the problem of uncertainty in projectiles flight parameter under different multiple projectile firing states.展开更多
The broad class of explosives includes nitro aromatics as well as challenging aliphatic nitro-organics whose detection is important from counter-terrorism and national security perspectives.Here we report a turn-on fl...The broad class of explosives includes nitro aromatics as well as challenging aliphatic nitro-organics whose detection is important from counter-terrorism and national security perspectives.Here we report a turn-on fluorescent sensor array based on aggregation-induced emission(AIE)fluorophores as receptors.To achieve a good sensing system with fast response,good sensitivity and low detection limit,three receptors with abundant chemical diversities for target analytes were synthesized.The turn-on response of the individual receptor showed highly variable and cross-reactive analyte-dependent changes in fluorescence.The excellent ability to identify a variety of explosives,especially the challenging aliphatic nitro-organics(2,3-dimethyl-2,3-dinitrobutane(DMNB),1,3,5-trinitro-1,3,5-triazinane(RDX),cyclotetramethylene tetranitramine(HMX)and entaerythritol tetranitrate(PETN)),was demonstrated in qualitative and quantitative analyses with 100%accuracy.The fluorescence signal amplification in the presence of explosives allows for application of these receptors in a sensor microarray suitable for high-throughput screening.These results suggested that the cross-reactive sensor array based on AIE fluorophores could find a wide range of applications for sensing various analytes or complex mixtures.展开更多
Pringe array is proposed as the cooperated target in the precise torsion angle detection. The target fringe array image is generated according to the structure of the optical system, and the torsion angle detection al...Pringe array is proposed as the cooperated target in the precise torsion angle detection. The target fringe array image is generated according to the structure of the optical system, and the torsion angle detection algorithm is analyzed in response to the gray distribution of the image. The factors affecting the detection precision of the fringe torsion angle are analyzed theoretically and numerically. It indicates that the detection precision of the torsion angle is 1 angular second or even less, carefully selecting the detector array. Significantly, experiments are performed to demonstrate the precision and the results match well with the simulations.展开更多
In the surface imaging of underwater structures, long working distance will reduce image quality due to the turbidity of water. To acquire high definition and large field of view(FOV) images for surface detection, a s...In the surface imaging of underwater structures, long working distance will reduce image quality due to the turbidity of water. To acquire high definition and large field of view(FOV) images for surface detection, a short-working-distance underwater imaging system is proposed based on camera array. A multi-view calibration and rectification method is developed. A look-up table(LUT) method and a multi-resolution spline(MRS) method are applied to stitch array images real-time and seamlessly.Experiments both in the air and in the water are conducted. Strength and weakness of the LUT and MRS methods are discussed.Based on the results, the effectiveness in surface detection of underwater structures is verified.展开更多
In this work, a smart strategy that combines three-way high performance liquid chromatography-diode array detection(HPLCDAD) data with second-order calibration method based on alternating trilinear decomposition(ATLD)...In this work, a smart strategy that combines three-way high performance liquid chromatography-diode array detection(HPLCDAD) data with second-order calibration method based on alternating trilinear decomposition(ATLD) algorithm was proposed for simultaneous determination of eleven non-steroidal anti-inflammatory drugs(NSAIDs) illegally added into Chinese patent drugs and health products. All target analytes were rapidly eluted out within 14.5 min under a simple gradient elution. With the aid of the prominent "second-order advantage" of the ATLD algorithm, three HPLC problems, i.e. peak overlaps, unknown interferences and baseline drift, could be mathematically calibrated, and pure signals of target analytes could be extracted out from heavy-interference but information-rich HPLC-DAD data. The average spiked recoveries for all target analytes were in the range of 95.9%–106.4% with standard deviations lower than 7.5%. Validation parameters including sensitivity(SEN), selectivity(SEL), limit of detection(LOD), limit of quantitation(LOQ) and precisions of intra-day and inter-day were calculated to validate the accuracy of the proposed method, quantitative results were further confirmed by the classic HPLC method, which proved that chemometrics-assisted HPLC-DAD analytical strategy was highly efficient, accurate and green for drug-abuse monitoring of NSAIDs in Chinese patent drugs and health products.展开更多
Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids(magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine)in Fufang Zhenzhu Tiao...Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids(magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine)in Fufang Zhenzhu Tiaozhi Capsule.Methods Chromatography was performed on a Dionex Acclaim C_(18)column(250 mm×4.6 mm,5.0μm)at 30℃.The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution(0.015 mol/L,40:60,including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0),with a flow rate of 1.0 mL/min.The detection wavelength was 270 nm.Results The calibration curves of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were linear in the range of 1.07-10.65,0.78-7.55,0.75-7.50,1.60-15.95,2.69-26.85,2.31-23.10,and 6.04-60.40 mg/mL.The average recoveries of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were 101.0%,101.2%,100.1%,100.0%,100.1%,101.1%,and 99.7%,respectively.Conclusion The method could be used for the quantitative determination of the preparation.展开更多
基金supported by the European Research Council,BrightEyes No.818699(E.S.,S.P.,M.O.H.,G.V.)the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No.890923(SMSPAD)(E.S.).
文摘The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy(SMLM)by overcoming the limit imposed by the fluorophore's photon counts.Typical MINFLUX microscopes localize the target molecule by scanning a zero-intensity focus around the molecule in a circular trajectory,with smaller trajectory diameters yielding better localization uncertainties for a given number of photons.Since this approach requires the molecule to be within the scanned trajectory,MINFLUX typically relies on an iterative scheme with decreasing trajectory diameters.This iterative approach is prone to misplacements of the trajectory and increases the system's complexity.In this work,we introduce ISM-FLUX,a novel implementation of MINFLUX using image-scanning microscopy(ISM)with a single-photon avalanche diode array detector.ISM-FLUX provides a precise MINFLUX localization within the trajectory while maintaining a conventional photon-limited uncertainty outside it.The robustness of ISM-FLUX localization results in a larger localization range and greatly simplifies the architecture,which may facilitate broader adoption of MINFLUX.
基金This work was supported by the National Natural Science Foundation of China(No.11804263)the Program for Innovative Science and Research Team of Xi’an Technological University.
文摘In order to improve the infrared detection and discrimination ability of the smart munition to the dynamic armor target under the complex background,the multi-line array infrared detection system is established based on the combination of the single unit infrared detector.The surface dimension features of ground armored targets are identified by size calculating solution algorithm.The signal response value and the value of size calculating are identified by the method of fuzzy recognition to make the fuzzy classification judgment for armored target.According to the characteristics of the target signal,a custom threshold de-noising function is proposed to solve the problem of signal preprocessing.The multi-line array infrared detection can complete the scanning detection in a large area in a short time with the characteristics of smart munition in the steady-state scanning stage.The method solves the disadvantages of wide scanning interval and low detection probability of single unit infrared detection.By reducing the scanning interval,the number of random rendezvous in the infrared feature area of the upper surface is increased,the accuracy of the size calculating is guaranteed.The experiments results show that in the fuzzy recognition method,the size calculating is introduced as the feature operator,which can improve the recognition ability of the ground armor target with different shape size.
基金supported by the National Natural Science Foundation of China (Nos. 62176214 and 61973253)。
文摘To address the problem of instability and inaccuracy when the Unmanned Aerial Vehicles(UAVs) formation equipped with bearing-only sensor network tracks a maneuvering target,this paper proposes a distributed cooperative tracking control method considering the effectiveness of passive detection. First, the system model of passive detection in UAV formation is constructed.Then, the Geometric Dilution of Precision(GDOP) of bearing-only sensor nodes pair on the observation plane is analyzed. Building on this foundation, the pairwise form is expanded to obtain the optimal geometric configuration for the entire network. Subsequently, the Distributed Cubature Information Filtering(DCIF) is integrated with the weighted average consensus protocol to design the distributed cooperative observer suitable for the system model, enabling state estimation of the target. Finally, within the distributed architecture, the Nonlinear Model Predictive Controller(NMPC) is designed. This controller autonomously assembles the UAV formation during the assembly phase and forms an optimal detection array. The UAV formation then tracks the target using the virtual geometric center based on the established rigid geometric configuration. The simulation experiments validate that the proposed model and method can enhance the passive detection effectiveness of the UAV formation, thereby achieving stable and efficient distributed cooperative tracking for the maneuvering target.
文摘Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.Methods
基金supported by China earthquake scientific array exploration Southern section of North South seismic belt(201008001)Northern section of North South seismic belt(20130811)+1 种基金National Natural Science Foundation of China(41474057)Science for Earthquake Resllience of China Earthquake Administration(XH15040Y)
文摘The special seismic tectonic environment and frequent seismicity in the southeastern margin of the Qinghai-Tibet Plateau show that this area is an ideal location to study the present tectonic movement and background of strong earthquakes in China's Mainland and to predict future strong earthquake risk zones.Studies of the structural environment and physical characteristics of the deep structure in this area are helpful to explore deep dynamic effects and deformation field characteristics,to strengthen our understanding of the roles of anisotropy and tectonic deformation and to study the deep tectonic background of the seismic origin of the block's interior.In this paper,the three-dimensional(3D)P-wave velocity structure of the crust and upper mantle under the southeastern margin of the Qinghai-Tibet Plateau is obtained via observational data from 224 permanent seismic stations in the regional digital seismic network of Yunnan and Sichuan Provinces and from 356 mobile China seismic arrays in the southern section of the north-south seismic belt using a joint inversion method of the regional earthquake and teleseismic data.The results indicate that the spatial distribution of the P-wave velocity anomalies in the shallow upper crust is closely related to the surface geological structure,terrain and lithology.Baoxing and Kangding,with their basic volcanic rocks and volcanic clastic rocks,present obvious high-velocity anomalies.The Chengdu Basin shows low-velocity anomalies associated with the Quaternary sediments.The Xichang Mesozoic Basin and the Butuo Basin are characterised by low-velocity anomalies related to very thick sedimentary layers.The upper and middle crust beneath the Chuan-Dian and Songpan-Ganzi Blocks has apparent lateral heterogeneities,including low-velocity zones of different sizes.There is a large range of low-velocity layers in the Songpan-Ganzi Block and the sub-block northwest of Sichuan Province,showing that the middle and lower crust is relatively weak.The Sichuan Basin,which is located in the western margin of the Yangtze platform,shows high-velocity characteristics.The results also reveal that there are continuous low-velocity layer distributions in the middle and lower crust of the Daliangshan Block and that the distribution direction of the low-velocity anomaly is nearly SN,which is consistent with the trend of the Daliangshan fault.The existence of the low-velocity layer in the crust also provides a deep source for the deep dynamic deformation and seismic activity of the Daliangshan Block and its boundary faults.The results of the 3D P-wave velocity structure show that an anomalous distribution of high-density,strong-magnetic and high-wave velocity exists inside the crust in the Panxi region.This is likely related to late Paleozoic mantle plume activity that led to a large number of mafic and ultra-mafic intrusions into the crust.In the crustal doming process,the massive intrusion of mantle-derived material enhanced the mechanical strength of the crustal medium.The P-wave velocity structure also revealed that the upper mantle contains a low-velocity layer at a depth of 80-120 km in the Panxi region.The existence of deep faults in the Panxi region,which provide conditions for transporting mantle thermal material into the crust,is the deep tectonic background for the area's strong earthquake activity.
基金supported both by the Natural Science Foundations of Hebei(No.B2008000210)the Scientific Research Foundation of Agricultural University of Hebei.
文摘A novel method for the determination of five carbamate pesticides （metolcarb, carbofuran, carbaryl, isoprocard and diethofencard） in water samples was developed by dispersive liquid-liquid microextraction （DLLME） coupled with high performance liquid chromatography-diode array detector （HPLC-DAD）. Some experimental parameters that influence the extraction efficiency were studied and optimized to obtain the best extraction results. Under the optimum conditions for the method, the calibration curve was linear in the concentration range from 5 to 1000 ng mL^-1 for all the five carbamate pesticides, with the correlation coefficients （r^2） varying from 0.9984 to 0.9994. Good enrichment factors were achieved ranging from 80 to 177- fold, depending on the compound. The limits of detection （LODs） （S/N = 3） were ranged from 0.1 to 0.5 ng mL^-1. The method has been successfully applied to the analysis of the pesticide residues in environmental water samples.
基金been supported by Project of the National Natural Science Foundation of China(No.62073256)the Shaanxi Provincial Science and Technology Department(No.2020GY-125)Xi’an Science and Technology Innovation talent service enterprise project(No.2020KJRC0041)。
文摘The dynamic parameters of multiple projectiles that are fired using multi-barrel weapons in highfrequency continuous firing modes are important indicators to measure the performance of these weapons.The characteristics of multiple projectiles are high randomness and large numbers launched in a short period of time,making it very difficult to obtain the real dispersion parameters of the projectiles due to the occlusion or coincidence of multiple projectiles.Using six intersecting-screen testing system,in this paper,we propose an association recognition and matching algorithm of multiple projectiles using a temporal and spatial information constraint mechanism.We extract the output signal from each detection screen and then use the wavelet transform to process the output signal.We present a method to identify and extract the time values on which the projectiles pass through the detection screens using the wavelet transform modulus maximum theory.We then use the correlation of the output signals of three parallel detection screens to establish a correlation coefficient recognition constraint function for the multiple projectiles.Based on the premise of linear projectile motion,we establish a temporal and spatial constraint matching model using the projectile’s position coordinates in each detection screen and the projectile’s time constraints within the multiple intersecting-screen geometry.We then determine the time values of the multiple projectiles in each detection screen using an iterative search cycle registration,and finally obtain the flight parameters for the multiple projectiles in the presence of uncertainty.The proposed method and algorithm were verified experimentally and can solve the problem of uncertainty in projectiles flight parameter under different multiple projectile firing states.
基金the National Natural Science Foundation of China(50873051,205333050)National High Technology Research and Development Program of China(2007AA03Z307)Transregional Project(TRR61)
文摘The broad class of explosives includes nitro aromatics as well as challenging aliphatic nitro-organics whose detection is important from counter-terrorism and national security perspectives.Here we report a turn-on fluorescent sensor array based on aggregation-induced emission(AIE)fluorophores as receptors.To achieve a good sensing system with fast response,good sensitivity and low detection limit,three receptors with abundant chemical diversities for target analytes were synthesized.The turn-on response of the individual receptor showed highly variable and cross-reactive analyte-dependent changes in fluorescence.The excellent ability to identify a variety of explosives,especially the challenging aliphatic nitro-organics(2,3-dimethyl-2,3-dinitrobutane(DMNB),1,3,5-trinitro-1,3,5-triazinane(RDX),cyclotetramethylene tetranitramine(HMX)and entaerythritol tetranitrate(PETN)),was demonstrated in qualitative and quantitative analyses with 100%accuracy.The fluorescence signal amplification in the presence of explosives allows for application of these receptors in a sensor microarray suitable for high-throughput screening.These results suggested that the cross-reactive sensor array based on AIE fluorophores could find a wide range of applications for sensing various analytes or complex mixtures.
基金the National Natural Science Foundation of China under Grant No.61275002.
文摘Pringe array is proposed as the cooperated target in the precise torsion angle detection. The target fringe array image is generated according to the structure of the optical system, and the torsion angle detection algorithm is analyzed in response to the gray distribution of the image. The factors affecting the detection precision of the fringe torsion angle are analyzed theoretically and numerically. It indicates that the detection precision of the torsion angle is 1 angular second or even less, carefully selecting the detector array. Significantly, experiments are performed to demonstrate the precision and the results match well with the simulations.
基金supported by the National Key Technology R&D Program(Grant No.2014BAK11B04)the National Natural Science Foundation of China(Grant Nos.11272089,11327201,11532005&11602056)
文摘In the surface imaging of underwater structures, long working distance will reduce image quality due to the turbidity of water. To acquire high definition and large field of view(FOV) images for surface detection, a short-working-distance underwater imaging system is proposed based on camera array. A multi-view calibration and rectification method is developed. A look-up table(LUT) method and a multi-resolution spline(MRS) method are applied to stitch array images real-time and seamlessly.Experiments both in the air and in the water are conducted. Strength and weakness of the LUT and MRS methods are discussed.Based on the results, the effectiveness in surface detection of underwater structures is verified.
基金supported by the National Natural Science Foundation of China (21575039, 21775039, 21521063)
文摘In this work, a smart strategy that combines three-way high performance liquid chromatography-diode array detection(HPLCDAD) data with second-order calibration method based on alternating trilinear decomposition(ATLD) algorithm was proposed for simultaneous determination of eleven non-steroidal anti-inflammatory drugs(NSAIDs) illegally added into Chinese patent drugs and health products. All target analytes were rapidly eluted out within 14.5 min under a simple gradient elution. With the aid of the prominent "second-order advantage" of the ATLD algorithm, three HPLC problems, i.e. peak overlaps, unknown interferences and baseline drift, could be mathematically calibrated, and pure signals of target analytes could be extracted out from heavy-interference but information-rich HPLC-DAD data. The average spiked recoveries for all target analytes were in the range of 95.9%–106.4% with standard deviations lower than 7.5%. Validation parameters including sensitivity(SEN), selectivity(SEL), limit of detection(LOD), limit of quantitation(LOQ) and precisions of intra-day and inter-day were calculated to validate the accuracy of the proposed method, quantitative results were further confirmed by the classic HPLC method, which proved that chemometrics-assisted HPLC-DAD analytical strategy was highly efficient, accurate and green for drug-abuse monitoring of NSAIDs in Chinese patent drugs and health products.
基金Cooperation Project in Industry,Education and Research of Guangdong Province and Ministry of Education of China(2009B090300349)a Cooperation Project of National Science Foundation of Guangdong Province(10351022401000000)
文摘Objective To establish a reverse-phase liquid chromatography method for the determination of seven alkaloids(magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine)in Fufang Zhenzhu Tiaozhi Capsule.Methods Chromatography was performed on a Dionex Acclaim C_(18)column(250 mm×4.6 mm,5.0μm)at 30℃.The mobile phase was composed of acetonitrile-potassium dihydrogen phosphate solution(0.015 mol/L,40:60,including 1.7 g/L sodium dodecyl sulfate and phosphoric acid used to regulate pH value to 3.0),with a flow rate of 1.0 mL/min.The detection wavelength was 270 nm.Results The calibration curves of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were linear in the range of 1.07-10.65,0.78-7.55,0.75-7.50,1.60-15.95,2.69-26.85,2.31-23.10,and 6.04-60.40 mg/mL.The average recoveries of magnoflorine,columbamine,jatrorrhizine,epiberberine,coptisine,palmatine,and berberine were 101.0%,101.2%,100.1%,100.0%,100.1%,101.1%,and 99.7%,respectively.Conclusion The method could be used for the quantitative determination of the preparation.
