To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip...To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.展开更多
A neuronal signal detecting circuit and a neuronal signal stimulating circuit designed for a monolithic integrated MEA（micro-electrode array） system are described. As a basic cell of the circuits, an OPA（ operation...A neuronal signal detecting circuit and a neuronal signal stimulating circuit designed for a monolithic integrated MEA（micro-electrode array） system are described. As a basic cell of the circuits, an OPA（ operational amplifier） is designed with low power, low noise, small size and high gain. The detecting circuit has a chip area of 290 μm × 400 μm, a power dissipation of 2.02 mW, an equivalent input noise of 17.72 nV/ Hz, a gain of 60. 5 dB, and an output voltage from - 2. 48 to ＋ 2. 5 V. The stimulating circuit has a chip area of 130 μm × 290 μm, a power dissipation of 740 μW, and an output voltage from - 2. 5 to 2. 04 V. The parameters show that two circuits are suitable for a monolithic integrated MEA system. The detecting circuit and MEA have been fabricated. The test results show that the detecting circuit works well.展开更多
Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with...Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with mercury film was used for simultaneous voltammetric detection of zinc,cadmium,lead and copper ions in water.The electrochemical behavior and the actual surface area of the MEA were investigated by cyclic voltammetry in K_(3)[Fe(CN)_(6)].Electrochemical impedance spectrum(EIS)was utilized to examine the deposition of mercury on the electrode surface.Based on anodic stripping voltammetry,mercury filmAu MEA was applied to the detection of heavy metals in artificial analyte,where good calibrate linearity was obtained for cadmium,lead and copper ions,but with zinc exhibiting poor linearity.展开更多
Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situat...Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situation,which is still a challenge to rapidly and uniformly establish though.Here,we propose a novel drug screening system,namely 3D tumor array chip with“layer cake”structure,for drug screening.Accurate gelatin methacryloyl hydrogel droplets(~0.1μL)containing tumor cells can be automatically deposited on demand with electrohydrodynamic 3D printing.Transparent conductive membrane is introduced as a chip basement for preventing charges accumulation during fabricating and convenient observing during screening.Culturing chambers formed by stainless steel and silicon interlayer is convenient to be assembled and recycled.As this chip is compatible with the existing 96-well culturing plate,the drug screening protocols could keep the same as convention.Important properties of this chip,namely printing stability,customizability,accuracy,microenvironment,tumor functionalization,are detailly examined.As a demonstration,it is applied for screening of epirubicin and paclitaxel with breast tumor cells to confirm the compatibility of the proposed screening system with the traditional screening methods.We believe this chip will potentially play a significant role in drug evaluation in the future.展开更多
We propose a wire configuration to create a one-dimensional （1D） array of magnetic microtraps for trapping ultracold atoms. The configuration is formed by replacing the central part of the Z-wire pattern with a zigz...We propose a wire configuration to create a one-dimensional （1D） array of magnetic microtraps for trapping ultracold atoms. The configuration is formed by replacing the central part of the Z-wire pattern with a zigzag wire. We simulate the performance of this pattern by the finite element method which can take both the width and depth of the wire into consideration. The result of simulation shows that this configuration can create magnetic microtraps which can be separated and combined by changing bias magnetic field. We manage to split Z-wire trap and prove that similar result can occur for the new wire configuration. The fabrication processes of the atom chip are also introduced. Finally we discuss the loading method.展开更多
Deep brain stimulation(DBS),including optical stimulation and electrical stimulation,has been demonstrated considerable value in exploring pathological brain activity and developing treatments for neural disorders.Adv...Deep brain stimulation(DBS),including optical stimulation and electrical stimulation,has been demonstrated considerable value in exploring pathological brain activity and developing treatments for neural disorders.Advances in DBS microsystems based on implantable microelectrode array(MEA)probes have opened up new opportunities for closed-loop DBS(CL-DBS)in situ.This technology can be used to detect damaged brain circuits and test the therapeutic potential for modulating the output of these circuits in a variety of diseases simultaneously.Despite the success and rapid utilization of MEA probe-based CL-DBS microsystems,key challenges,including excessive wired communication,need to be urgently resolved.In this review,we considered recent advances in MEA probe-based wireless CL-DBS microsystems and outlined the major issues and promising prospects in this field.This technology has the potential to offer novel therapeutic options for psychiatric disorders in the future.展开更多
A microelectrode array（MEA） is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair ...A microelectrode array（MEA） is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board （PCB） technology. The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal （AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz）. The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro.展开更多
AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybri...AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybridized by oligonucleotide microarrays.RESULTS： One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 （96.2%） were identified.CONCLUSION： Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus , Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonudeotides to the arrays without any significant increase of complexity or cost.展开更多
On-chip couplers are essential for coupling free-space electromagnetic waves into sub-wavelength semiconductor devices and enhancing light-matter interactions.However,the couplers used in existing single field-effect ...On-chip couplers are essential for coupling free-space electromagnetic waves into sub-wavelength semiconductor devices and enhancing light-matter interactions.However,the couplers used in existing single field-effect transistor(FET)detectors exhibit poor response over wide frequency ranges,making the detection of ultra-wideband weak signals highly challenging.In this work,we introduce a meta-array coupler for terahertz detectors based on field-effect transistors,which demonstrates exceptional coupling asymmetry beneath the gate.To optimize the meta-array structure for high gain across an ultra-wide frequency range,we propose a hybrid long short-term memory–multi-layer perceptron(LSTM-MLP)neural network for the detector’s on-chip coupler.This method enables accurate and rapid prediction of the enhanced spectra of meta-atoms,facilitating efficient conversion from structural parameters to resonance frequencies.Compared to traditional dipole couplers,the proposed metacoupler generates strong localized electric field enhancement through dipole resonance,achieving a mixing factor asymmetry up to 246 times higher near 660 GHz and an antenna factor enhancement of approximately 50 times.The meta-array coupled FET terahertz detector achieves ultra-wideband resonance enhancement from 350 to1000 GHz.This hardware architecture and hybrid LSTM-MLP neural network are expected to overcome the limitations of current FET-based detectors in coupling efficiency,bridging the performance gap toward ultra-sensitive detection applications.展开更多
BACKGROUND: The relationship between cytokines and responses to peginterferon α-2a treatment in chronic hepatitis B patients has not yet been fully elucidated. We analyzed the serum levels of interleukin (IL)-1α, I...BACKGROUND: The relationship between cytokines and responses to peginterferon α-2a treatment in chronic hepatitis B patients has not yet been fully elucidated. We analyzed the serum levels of interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, vascular endothelial growth factor, interferon-γ, tumor necrosis factor-α, monocyte chemotactic protein-1 (MCP1) and epidermal growth factor during the treatment with peginterferon α-2a. METHODS: Ninety-three serum samples from 20 chronic hepatitis B patients were collected before, during and after 48 weeks of peginterferon therapy and were assayed for 12 cytokines. The patients were categorized as either virologic responders (VRs) or non-responders (NRs) according to their HBV DNA levels taken at 6th month during treatment. The Evidence Investigator (Randox, Antrim, UK), a protein chip analyzer, was used to quantify cytokines. RESULTS: Among the 12 cytokines, the levels of MCP1 were increased and the levels of IL-4 were decreased during the treatment in VRs. However these cytokines were not significantly changed in NRs in the treatment phases. Area under the receiver operating characteristic curve (AUROC) value of HBV DNA measured before the treatment was 0.81 in predicting VRs, and that of the baseline MCP1 was 0.76. IL-6 levels at 3rd and 6th months during the treatment also showed AUROC values 0.85 and 0.78 respectively in predicting sustained VRs. CONCLUSION: Serum cytokine levels reflect the pathological differences of individual treatment phases and could also be useful in monitoring responses to peginterferon treatment in chronic hepatitis B patients.展开更多
A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC...A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC）, moving target detection （MTD）, constant false alarm rate （CFAR） and target dots processing. Preliminary target dots information is obtained in PC, MTD, and CFAR modules and Nios I! CPU is used for target dots combination and false sidelobe target removing. Sys- tem on programmable chip （SOPC） technique is adopted in the system in which SDRAM is used to cache data. Finally, a FPGA-based binary phase coded radar signal processor is realized and simula- tion result is given.展开更多
Electron cyclotron resonance heating (ECRH) system is one of the most important Tokamak auxiliary heating methods. However, there are growing demands for ECRH system as the physical experiments progress which meanwhil...Electron cyclotron resonance heating (ECRH) system is one of the most important Tokamak auxiliary heating methods. However, there are growing demands for ECRH system as the physical experiments progress which meanwhile adds the difficulty of designing and building the control system of its power source. In this paper, the method of designing a control system based on Single Chip Microcomputer (SCM) and Field Programmable Gate Array (FPGA) is introduced according to its main requirements. The experimental results show that the control system in this paper achieves the conversion of different working modes, gets exact timing, and realizes the failure protection in 10us thus can be used in the ECRH system.展开更多
In modern manufacturing equipment control area,controller is required to deliver higher computing capability for adopting advanced algorithms to meet speed and accuracy requirements,and reconfigurabilities for changin...In modern manufacturing equipment control area,controller is required to deliver higher computing capability for adopting advanced algorithms to meet speed and accuracy requirements,and reconfigurabilities for changing or(and)adding features or functions.This paper presents a methodology in design and implementation of a high performance and reconfigurable platform for manufacturing equipment control.This methodology is in virtue of system on a programmable chip(SoPC)technolo- gy but replacing the on-chip processor by an external high performance,floating-point digital signal processor(DSP).The appli- cation of the DSP is designed as a multi-threaded framework,which has more flexibilities than a traditional single-loop one.Fur- thermore,the field programmable gate array(FPGA)system can be reconfigured easily and quickly to meet a new requirement by dragging and dropping pre-built components in a SoPC building environment.As a result,the controller platform is more recon- figurable in terms of algorithms and functions.This platform is implemented in a 3-axis milling machine control and the result indicates that the design and implementation presented in this paper is feasible.展开更多
The design of iterative learning controller(ILC) requires to store the system input, output or control parameters of previous trials for generating the input of the current trial. In order to apply the iterative learn...The design of iterative learning controller(ILC) requires to store the system input, output or control parameters of previous trials for generating the input of the current trial. In order to apply the iterative learning controller for a real application and reduce the memory size for implementation, a current error based sampled-data proportional-derivative(PD) type iterative learning controller is proposed for control systems with initial resetting error, input disturbance and output measurement noise in this paper.The proposed iterative learning controller is simple and effective. The first contribution in this paper is to prove the learning error convergence via a rigorous technical analysis. It is shown that the learning error will converge to a residual set if a forgetting factor is introduced in the controller. All the theoretical results are also shown by computer simulations. The second main contribution is to realize the iterative learning controller by a digital circuit using a field programmable gate array(FPGA) chip applied to repetitive position tracking control of direct current(DC) motors. The feasibility and effectiveness of the proposed current error based sampleddata iterative learning controller are demonstrated by the experiment results. Finally, the relationship between learning performance and design parameters are also discussed extensively.展开更多
Time-modulated array(TMA)antennas,introduce the dimension of time into antenna design to control the radiation patterns and frequency spectral characteristics,thus improve the reconfigurability of array antennas and p...Time-modulated array(TMA)antennas,introduce the dimension of time into antenna design to control the radiation patterns and frequency spectral characteristics,thus improve the reconfigurability of array antennas and provide multiple functional-ities.They have great application potential in military and civilian fields,such as precision guidance and mobile communication,and are currently a hot spot of academic research.This article provides a review on the fundamentals and applications of TMAs.First,the basic theory and mathematical formulations of TMAs are introduced.Second,the most important applications of TMAs,namely time-modulated phased arrays(TMPA),are discussed from the perspectives of harmonic suppression and harmonic utiliza-tion,which are used for single-beam and multibeam radiation.Then,we survey the combination of TMA with various types of novel antenna arrays,such as single-channel digital beamforming(DBF)arrays,frequency diverse arrays(FDAs),and retrodirective arrays,to create new hardware implementation methods and enhance their performance.Next,recent advances in dedicated integrated chips for TMA,which have played a significant role in driving the progress of TMAs from academic research to practical applications,are presented.Finally,the challenges and prospects for TMAs are discussed,including new research directions and emerging applica-tion scenarios.展开更多
The membrane electrode assembly(MEA)plays a crucial role in the functionality of proton exchange membrane fuel cells(PEMFCs).The channels present within the catalyst layer of MEAs exhibit a disordered configuration,wh...The membrane electrode assembly(MEA)plays a crucial role in the functionality of proton exchange membrane fuel cells(PEMFCs).The channels present within the catalyst layer of MEAs exhibit a disordered configuration,which consequently give rise to low efficiency in mass transportation.In order to enhance the mass transfer performance and the corrosion resistance of the catalyst layer,this paper developed a double-side ordered MEA based on TiN nanorod arrays.We synthesized TiN nanorod arrays on the ITO surface by a seed-assisted hydrothermal reaction and nitriding treatment,and coated the catalyst uniformly on the TiN support by ultrasonic spraying.Then the double-side ordered MEA was fabricated by transfer printing,and achieved a peak power of 678.30 mW cm^(-2) with a cathode platinum loading of 0.2 mg cm^(-2) at 80℃ and anode saturated humidity.After 200 hours of accelerated stress test(AST)at 90℃ and 30/30%relative humidity,the peak performance only dropped by 4.8%.These results provide substantial evidence for the effectiveness of our developed double-side ordered MEA which can mitigate catalyst polarization corrosion.Thus,this study reveals the immense potential of the TiN nanorod array-based double-side ordered MEA in advancing the development of efficient and stable MEAs.展开更多
基金sponsored by the National Natural Science Foundation of China (Grant Nos.61960206012,62121003,T2293731,62171434,61975206,61971400,and 61973292)the National Key Research and Development Program of China (Grant Nos.2022YFB3205602 and 2022YFC2402501)+1 种基金Major Program of Scientific and Technical Innovation 2030 (Grant No.2021ZD02016030)the Scientific Instrument Developing Project of the Chinese Academy of Sciences (Grant No.GJJSTD20210004).
文摘To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.
基金The National Natural Science Foundation of China (No.90307013,90707005)the Natural Science Foundation of Jiangsu Province(No. BK2008032)Open Foundation of State Key Laboratory of Bio-Electronics of Southeast University
文摘A neuronal signal detecting circuit and a neuronal signal stimulating circuit designed for a monolithic integrated MEA（micro-electrode array） system are described. As a basic cell of the circuits, an OPA（ operational amplifier） is designed with low power, low noise, small size and high gain. The detecting circuit has a chip area of 290 μm × 400 μm, a power dissipation of 2.02 mW, an equivalent input noise of 17.72 nV/ Hz, a gain of 60. 5 dB, and an output voltage from - 2. 48 to ＋ 2. 5 V. The stimulating circuit has a chip area of 130 μm × 290 μm, a power dissipation of 740 μW, and an output voltage from - 2. 5 to 2. 04 V. The parameters show that two circuits are suitable for a monolithic integrated MEA system. The detecting circuit and MEA have been fabricated. The test results show that the detecting circuit works well.
基金This work has been supported by grants from the National Basic Research Program of China(973 program),Grant No.2009CB320303.
文摘Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with mercury film was used for simultaneous voltammetric detection of zinc,cadmium,lead and copper ions in water.The electrochemical behavior and the actual surface area of the MEA were investigated by cyclic voltammetry in K_(3)[Fe(CN)_(6)].Electrochemical impedance spectrum(EIS)was utilized to examine the deposition of mercury on the electrode surface.Based on anodic stripping voltammetry,mercury filmAu MEA was applied to the detection of heavy metals in artificial analyte,where good calibrate linearity was obtained for cadmium,lead and copper ions,but with zinc exhibiting poor linearity.
基金This work was sponsored by the National Nature Science Foundation of China(No.U1609207)the National Key Research and Development Program of China(2018YFA0703000)the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.51521064).
文摘Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situation,which is still a challenge to rapidly and uniformly establish though.Here,we propose a novel drug screening system,namely 3D tumor array chip with“layer cake”structure,for drug screening.Accurate gelatin methacryloyl hydrogel droplets(~0.1μL)containing tumor cells can be automatically deposited on demand with electrohydrodynamic 3D printing.Transparent conductive membrane is introduced as a chip basement for preventing charges accumulation during fabricating and convenient observing during screening.Culturing chambers formed by stainless steel and silicon interlayer is convenient to be assembled and recycled.As this chip is compatible with the existing 96-well culturing plate,the drug screening protocols could keep the same as convention.Important properties of this chip,namely printing stability,customizability,accuracy,microenvironment,tumor functionalization,are detailly examined.As a demonstration,it is applied for screening of epirubicin and paclitaxel with breast tumor cells to confirm the compatibility of the proposed screening system with the traditional screening methods.We believe this chip will potentially play a significant role in drug evaluation in the future.
基金Project supported by the National Basic Research Program of China(Grant No.2006CB921202)the National Natural Science Foundation of China(Grant No.10974210)
文摘We propose a wire configuration to create a one-dimensional （1D） array of magnetic microtraps for trapping ultracold atoms. The configuration is formed by replacing the central part of the Z-wire pattern with a zigzag wire. We simulate the performance of this pattern by the finite element method which can take both the width and depth of the wire into consideration. The result of simulation shows that this configuration can create magnetic microtraps which can be separated and combined by changing bias magnetic field. We manage to split Z-wire trap and prove that similar result can occur for the new wire configuration. The fabrication processes of the atom chip are also introduced. Finally we discuss the loading method.
基金supported by the National Natural Science Foundation of China(Nos.T2293730,T2293731,62121003,61960206012,61973292,62171434,61975206,and 61971400)the National Key Research and Development Program of China(Nos.2022YFC2402501 and 2022YFB3205602)+1 种基金the Major Program of Scientific and Technical Innovation 2030(No.2021ZD02016030)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.GJJSTD20210004).
文摘Deep brain stimulation(DBS),including optical stimulation and electrical stimulation,has been demonstrated considerable value in exploring pathological brain activity and developing treatments for neural disorders.Advances in DBS microsystems based on implantable microelectrode array(MEA)probes have opened up new opportunities for closed-loop DBS(CL-DBS)in situ.This technology can be used to detect damaged brain circuits and test the therapeutic potential for modulating the output of these circuits in a variety of diseases simultaneously.Despite the success and rapid utilization of MEA probe-based CL-DBS microsystems,key challenges,including excessive wired communication,need to be urgently resolved.In this review,we considered recent advances in MEA probe-based wireless CL-DBS microsystems and outlined the major issues and promising prospects in this field.This technology has the potential to offer novel therapeutic options for psychiatric disorders in the future.
基金The National Natural Science Foundation of China(No. 61076118, 90307013, 90707005)the Natural Science Foundation of Jiangsu Province (No. BK2008032)Special Foundation and Open Foundation of the State Key Laboratory of Bioelectronics of Southeast University
文摘A microelectrode array（MEA） is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board （PCB） technology. The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal （AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz）. The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro.
基金Supported by the National High Technology ResearchDevelopment Program of China (863 Program), No.2002AA2Z2011
文摘AIM： To detect the common intestinal pathogenic bacteria quickly and accurately.METHODS： A rapid （〈3 h） experimental procedure was set up based upon the gene chip technology, Target genes were amplified and hybridized by oligonucleotide microarrays.RESULTS： One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 （96.2%） were identified.CONCLUSION： Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus , Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonudeotides to the arrays without any significant increase of complexity or cost.
基金National Key Research and Development Program of China(2023YFB3207800)National Natural Science Foundation of China(61927804)。
文摘On-chip couplers are essential for coupling free-space electromagnetic waves into sub-wavelength semiconductor devices and enhancing light-matter interactions.However,the couplers used in existing single field-effect transistor(FET)detectors exhibit poor response over wide frequency ranges,making the detection of ultra-wideband weak signals highly challenging.In this work,we introduce a meta-array coupler for terahertz detectors based on field-effect transistors,which demonstrates exceptional coupling asymmetry beneath the gate.To optimize the meta-array structure for high gain across an ultra-wide frequency range,we propose a hybrid long short-term memory–multi-layer perceptron(LSTM-MLP)neural network for the detector’s on-chip coupler.This method enables accurate and rapid prediction of the enhanced spectra of meta-atoms,facilitating efficient conversion from structural parameters to resonance frequencies.Compared to traditional dipole couplers,the proposed metacoupler generates strong localized electric field enhancement through dipole resonance,achieving a mixing factor asymmetry up to 246 times higher near 660 GHz and an antenna factor enhancement of approximately 50 times.The meta-array coupled FET terahertz detector achieves ultra-wideband resonance enhancement from 350 to1000 GHz.This hardware architecture and hybrid LSTM-MLP neural network are expected to overcome the limitations of current FET-based detectors in coupling efficiency,bridging the performance gap toward ultra-sensitive detection applications.
基金supported by a faculty research grant of Yonsei University College of Medicine for 2006(6-2006-0080)
文摘BACKGROUND: The relationship between cytokines and responses to peginterferon α-2a treatment in chronic hepatitis B patients has not yet been fully elucidated. We analyzed the serum levels of interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, vascular endothelial growth factor, interferon-γ, tumor necrosis factor-α, monocyte chemotactic protein-1 (MCP1) and epidermal growth factor during the treatment with peginterferon α-2a. METHODS: Ninety-three serum samples from 20 chronic hepatitis B patients were collected before, during and after 48 weeks of peginterferon therapy and were assayed for 12 cytokines. The patients were categorized as either virologic responders (VRs) or non-responders (NRs) according to their HBV DNA levels taken at 6th month during treatment. The Evidence Investigator (Randox, Antrim, UK), a protein chip analyzer, was used to quantify cytokines. RESULTS: Among the 12 cytokines, the levels of MCP1 were increased and the levels of IL-4 were decreased during the treatment in VRs. However these cytokines were not significantly changed in NRs in the treatment phases. Area under the receiver operating characteristic curve (AUROC) value of HBV DNA measured before the treatment was 0.81 in predicting VRs, and that of the baseline MCP1 was 0.76. IL-6 levels at 3rd and 6th months during the treatment also showed AUROC values 0.85 and 0.78 respectively in predicting sustained VRs. CONCLUSION: Serum cytokine levels reflect the pathological differences of individual treatment phases and could also be useful in monitoring responses to peginterferon treatment in chronic hepatitis B patients.
基金Supported by the Ministerial Level Advanced Research Foundation (SP240012)
文摘A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC）, moving target detection （MTD）, constant false alarm rate （CFAR） and target dots processing. Preliminary target dots information is obtained in PC, MTD, and CFAR modules and Nios I! CPU is used for target dots combination and false sidelobe target removing. Sys- tem on programmable chip （SOPC） technique is adopted in the system in which SDRAM is used to cache data. Finally, a FPGA-based binary phase coded radar signal processor is realized and simula- tion result is given.
文摘Electron cyclotron resonance heating (ECRH) system is one of the most important Tokamak auxiliary heating methods. However, there are growing demands for ECRH system as the physical experiments progress which meanwhile adds the difficulty of designing and building the control system of its power source. In this paper, the method of designing a control system based on Single Chip Microcomputer (SCM) and Field Programmable Gate Array (FPGA) is introduced according to its main requirements. The experimental results show that the control system in this paper achieves the conversion of different working modes, gets exact timing, and realizes the failure protection in 10us thus can be used in the ECRH system.
基金Supported by the Foundation:Guangdong Provincial Science and Technology Committee under Grant No.2002C1020407.
文摘In modern manufacturing equipment control area,controller is required to deliver higher computing capability for adopting advanced algorithms to meet speed and accuracy requirements,and reconfigurabilities for changing or(and)adding features or functions.This paper presents a methodology in design and implementation of a high performance and reconfigurable platform for manufacturing equipment control.This methodology is in virtue of system on a programmable chip(SoPC)technolo- gy but replacing the on-chip processor by an external high performance,floating-point digital signal processor(DSP).The appli- cation of the DSP is designed as a multi-threaded framework,which has more flexibilities than a traditional single-loop one.Fur- thermore,the field programmable gate array(FPGA)system can be reconfigured easily and quickly to meet a new requirement by dragging and dropping pre-built components in a SoPC building environment.As a result,the controller platform is more recon- figurable in terms of algorithms and functions.This platform is implemented in a 3-axis milling machine control and the result indicates that the design and implementation presented in this paper is feasible.
基金supported by National Science Council,Taiwan,China(No.NSC102-2221-E-211-011)National Nature Science Foundation of China(No.61374102)
文摘The design of iterative learning controller(ILC) requires to store the system input, output or control parameters of previous trials for generating the input of the current trial. In order to apply the iterative learning controller for a real application and reduce the memory size for implementation, a current error based sampled-data proportional-derivative(PD) type iterative learning controller is proposed for control systems with initial resetting error, input disturbance and output measurement noise in this paper.The proposed iterative learning controller is simple and effective. The first contribution in this paper is to prove the learning error convergence via a rigorous technical analysis. It is shown that the learning error will converge to a residual set if a forgetting factor is introduced in the controller. All the theoretical results are also shown by computer simulations. The second main contribution is to realize the iterative learning controller by a digital circuit using a field programmable gate array(FPGA) chip applied to repetitive position tracking control of direct current(DC) motors. The feasibility and effectiveness of the proposed current error based sampleddata iterative learning controller are demonstrated by the experiment results. Finally, the relationship between learning performance and design parameters are also discussed extensively.
基金supported by the National Natural Science Foundation of China(Grant Nos.62101258,62071235 and 62271260)the Jiangsu Province Science&Technology Department(Grant No.BE2021017).
文摘Time-modulated array(TMA)antennas,introduce the dimension of time into antenna design to control the radiation patterns and frequency spectral characteristics,thus improve the reconfigurability of array antennas and provide multiple functional-ities.They have great application potential in military and civilian fields,such as precision guidance and mobile communication,and are currently a hot spot of academic research.This article provides a review on the fundamentals and applications of TMAs.First,the basic theory and mathematical formulations of TMAs are introduced.Second,the most important applications of TMAs,namely time-modulated phased arrays(TMPA),are discussed from the perspectives of harmonic suppression and harmonic utiliza-tion,which are used for single-beam and multibeam radiation.Then,we survey the combination of TMA with various types of novel antenna arrays,such as single-channel digital beamforming(DBF)arrays,frequency diverse arrays(FDAs),and retrodirective arrays,to create new hardware implementation methods and enhance their performance.Next,recent advances in dedicated integrated chips for TMA,which have played a significant role in driving the progress of TMAs from academic research to practical applications,are presented.Finally,the challenges and prospects for TMAs are discussed,including new research directions and emerging applica-tion scenarios.
基金The authors acknowledge the financial support from the National Natural Science Foundation of China(U1809220)Key Research and Development Program of Zhejiang Province(2022C01113)Key Research and Fundamental Research Funds for the Central Universities(2022FZZX01-06).
文摘The membrane electrode assembly(MEA)plays a crucial role in the functionality of proton exchange membrane fuel cells(PEMFCs).The channels present within the catalyst layer of MEAs exhibit a disordered configuration,which consequently give rise to low efficiency in mass transportation.In order to enhance the mass transfer performance and the corrosion resistance of the catalyst layer,this paper developed a double-side ordered MEA based on TiN nanorod arrays.We synthesized TiN nanorod arrays on the ITO surface by a seed-assisted hydrothermal reaction and nitriding treatment,and coated the catalyst uniformly on the TiN support by ultrasonic spraying.Then the double-side ordered MEA was fabricated by transfer printing,and achieved a peak power of 678.30 mW cm^(-2) with a cathode platinum loading of 0.2 mg cm^(-2) at 80℃ and anode saturated humidity.After 200 hours of accelerated stress test(AST)at 90℃ and 30/30%relative humidity,the peak performance only dropped by 4.8%.These results provide substantial evidence for the effectiveness of our developed double-side ordered MEA which can mitigate catalyst polarization corrosion.Thus,this study reveals the immense potential of the TiN nanorod array-based double-side ordered MEA in advancing the development of efficient and stable MEAs.
