The embryogenesis, pollen germination, floral character and seed physiology of the endangered plant Manglietia aromatica Dandy were investigated. Based on this study, this species has very low seed set rate. The abort...The embryogenesis, pollen germination, floral character and seed physiology of the endangered plant Manglietia aromatica Dandy were investigated. Based on this study, this species has very low seed set rate. The abortion rate of functional megaspores in all the ovules is 27.9%, the egg cell abortion rate of mature embryo sacs is up to 80%, and the germination rate of pollen grains is as low as nearly 0.01%. In addition, the floral structure appears to be another limited factor for the effective pollination of this species. The endangerment mechanism of this species seems to be comprehensive. Human's destroying actions are the direct factors that have made the population degenerate quickly; low reproductive ability and the destroyed environments are the main reasons that prevent the population from renovating and spreading. Therefore, the conservation measures suggested by this study are to research the breed technology, artificial population renovating, in situ conservation, and ex situ conservation.展开更多
AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO...AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.展开更多
【目的】给香青藤(Illigera aromatica S.Z.Huang et S.L.Mo)的资源开发、质量控制体系构建及临床合理应用提供科学依据,也为壮药特色资源的现代化研究奠定基础。【方法】综述了香青藤已报道的化学成分,涵盖生物碱类、萜类、黄酮类、挥...【目的】给香青藤(Illigera aromatica S.Z.Huang et S.L.Mo)的资源开发、质量控制体系构建及临床合理应用提供科学依据,也为壮药特色资源的现代化研究奠定基础。【方法】综述了香青藤已报道的化学成分,涵盖生物碱类、萜类、黄酮类、挥发油类及甾体类等,结合其抗肿瘤、镇痛抗炎、心脑血管保护等药理作用研究进展,基于中药质量标志物(Q-Marker)理论,从植物亲缘学及化学成分特有性、化学成分与传统药效关联性、化学成分可测性3个维度,预测阿朴啡类生物碱、肉桂酸苯乙酯可作为香青藤质量标志物。【结论】国内外对青藤属植物研究较多,但针对香青藤的研究主要集中于生药学及挥发油成分分析,其化学成分系统辨识、药效物质基础、作用机制等研究仍不够深入,临床应用范围亦局限于广西地区,与其特有资源优势不符。展开更多
本研究收集了10份姜黄属(Curcuma)种质资源,应用32对SSR标记对其进行了遗传多样性和系统发育分析,一共检测到116个多态性位点,引物多态信息含量(polymorphism information content,PIC)平均值为0.6143,观测杂合度(observed heterozygosi...本研究收集了10份姜黄属(Curcuma)种质资源,应用32对SSR标记对其进行了遗传多样性和系统发育分析,一共检测到116个多态性位点,引物多态信息含量(polymorphism information content,PIC)平均值为0.6143,观测杂合度(observed heterozygosity,Ho)平均值为0.5102,期望杂合度(expected heterozygosity,He)平均值为0.6432。Shannon遗传多样性指数(Shannon's information index,I)平均值为1.0802;Nei's遗传多样性指数(Nei's genetic diversity index,Nei)平均值为0.6008,平均遗传分化系数(genetic differentiation,Fst)为0.1886。通过亲缘关系分析与主坐标分析,10份姜黄属资源分为2个亚群,亚群间基因流(gene flow,Nm)为1.0752。结果表明,10份姜黄属种质资源具有较高的遗传多样性,本研究有利于姜黄属植物的鉴定及遗传研究。展开更多
文摘The embryogenesis, pollen germination, floral character and seed physiology of the endangered plant Manglietia aromatica Dandy were investigated. Based on this study, this species has very low seed set rate. The abortion rate of functional megaspores in all the ovules is 27.9%, the egg cell abortion rate of mature embryo sacs is up to 80%, and the germination rate of pollen grains is as low as nearly 0.01%. In addition, the floral structure appears to be another limited factor for the effective pollination of this species. The endangerment mechanism of this species seems to be comprehensive. Human's destroying actions are the direct factors that have made the population degenerate quickly; low reproductive ability and the destroyed environments are the main reasons that prevent the population from renovating and spreading. Therefore, the conservation measures suggested by this study are to research the breed technology, artificial population renovating, in situ conservation, and ex situ conservation.
基金National key project of the 9~(th) 5-year Plan for Medicine and Health,No.96-906-07-04Guangdong provincial natural scientific grants,No.980663.
文摘AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.
文摘【目的】给香青藤(Illigera aromatica S.Z.Huang et S.L.Mo)的资源开发、质量控制体系构建及临床合理应用提供科学依据,也为壮药特色资源的现代化研究奠定基础。【方法】综述了香青藤已报道的化学成分,涵盖生物碱类、萜类、黄酮类、挥发油类及甾体类等,结合其抗肿瘤、镇痛抗炎、心脑血管保护等药理作用研究进展,基于中药质量标志物(Q-Marker)理论,从植物亲缘学及化学成分特有性、化学成分与传统药效关联性、化学成分可测性3个维度,预测阿朴啡类生物碱、肉桂酸苯乙酯可作为香青藤质量标志物。【结论】国内外对青藤属植物研究较多,但针对香青藤的研究主要集中于生药学及挥发油成分分析,其化学成分系统辨识、药效物质基础、作用机制等研究仍不够深入,临床应用范围亦局限于广西地区,与其特有资源优势不符。
