Salmonellosis represents a global epidemic,and the emergence of extensively drug-resistant(XDR)Salmonella and its sustained transmission worldwide constitutes a significant public health concern.Flagellum-mediated mot...Salmonellosis represents a global epidemic,and the emergence of extensively drug-resistant(XDR)Salmonella and its sustained transmission worldwide constitutes a significant public health concern.Flagellum-mediated motility serves as a crucial virulence trait of Salmonella that guides the pathogen toward the epithelial surface,enhancing gut colonization.Artemisia argyit essential oil,a traditional herb extract,demonstrates efficacy in treating inflammationrelated symptoms and diseases;however,its effects on flagellum assembly and expression mechanisms in anti-Salmonella activity remain inadequately explored.This study aimed to elucidate the mechanism by which Artemisia argyit essential oil addresses Salmonella infections.Network pharmacological analysis revealed that Traditional Chinese Medicine(TCM)Artemisia argyit exhibited anti-Salmonella infection potential and inhibited flagellum-dependent motility.The application of Artemisia argyit essential oil induced notable motility defects through the downregulation of flagellar and fimbriae expression.Moreover,it significantly reduced Salmonella-infected cell damage by interfering with flagellum-mediated Salmonella colonization.In vivo studies demonstrated that Artemisia argyit essential oil administration effectively alleviated Salmonella infection symptoms by reducing bacterial loads,inhibiting interleukin-1 beta(IL-1β),IL-6,and tumor necrosis factor-alpha(TNF-α)production,and diminishing pathological injury.Gas chromatography-mass spectrometry(GC-MS)analysis identified forty-three compounds in Artemisia argyit essential oil,with their corresponding targets and active ingredients predicted.Investigation of an in vivo model of Salmonella infection using the active ingredient demonstrated that alpha-cedrene ameliorated Salmonella infection.These findings suggest the potential application of Artemisia argyit essential oil in controlling Salmonella,the predominant food-borne pathogen.展开更多
基金the National Natural Science Foundation of China(Nos.U22A20523,U23A20242)National Key Research and Development Program of China(No.2021YFD1801000).
文摘Salmonellosis represents a global epidemic,and the emergence of extensively drug-resistant(XDR)Salmonella and its sustained transmission worldwide constitutes a significant public health concern.Flagellum-mediated motility serves as a crucial virulence trait of Salmonella that guides the pathogen toward the epithelial surface,enhancing gut colonization.Artemisia argyit essential oil,a traditional herb extract,demonstrates efficacy in treating inflammationrelated symptoms and diseases;however,its effects on flagellum assembly and expression mechanisms in anti-Salmonella activity remain inadequately explored.This study aimed to elucidate the mechanism by which Artemisia argyit essential oil addresses Salmonella infections.Network pharmacological analysis revealed that Traditional Chinese Medicine(TCM)Artemisia argyit exhibited anti-Salmonella infection potential and inhibited flagellum-dependent motility.The application of Artemisia argyit essential oil induced notable motility defects through the downregulation of flagellar and fimbriae expression.Moreover,it significantly reduced Salmonella-infected cell damage by interfering with flagellum-mediated Salmonella colonization.In vivo studies demonstrated that Artemisia argyit essential oil administration effectively alleviated Salmonella infection symptoms by reducing bacterial loads,inhibiting interleukin-1 beta(IL-1β),IL-6,and tumor necrosis factor-alpha(TNF-α)production,and diminishing pathological injury.Gas chromatography-mass spectrometry(GC-MS)analysis identified forty-three compounds in Artemisia argyit essential oil,with their corresponding targets and active ingredients predicted.Investigation of an in vivo model of Salmonella infection using the active ingredient demonstrated that alpha-cedrene ameliorated Salmonella infection.These findings suggest the potential application of Artemisia argyit essential oil in controlling Salmonella,the predominant food-borne pathogen.
