Artemisia argyi(A.argyi)is a Chinese herbal medicine with reported anti-inflammatory effects.In this study,the A.argyi was extracted with water and ethanol,and the concentrations of 35 flavonoids in A.argyi water extr...Artemisia argyi(A.argyi)is a Chinese herbal medicine with reported anti-inflammatory effects.In this study,the A.argyi was extracted with water and ethanol,and the concentrations of 35 flavonoids in A.argyi water extract(WE)and ethanol extract(EE)were measured via targeted metabolomics.The antioxidant and antiinflammatory activities of both WE and EE were firstly explored in vitro via chemical assays and cellular experiment,respectively.Both WE and EE showed significant 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),·OH,and O_(2)·radical scavenging ability in a dose-dependent manner,and reduced the levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-22(IL-22)in lipopolysaccharide(LPS)induced RAW264.7 cell model.In addition,the in vivo anti-colitis activity of both extracts was investigated in dextran sulfate sodium(DSS)-induced colitis mice,and the underlying mechanisms were elucidated by 16S r DNA sequencing and targeted metabolomics.We found that both WE and EE relieved colitis in mice,characterized by decreased disease activity index,increased colon length,improved pathological changes in colon tissue,while EE showed better anti-colitis activity.In addition,both 16S r DNA sequencing and targeted bile acids metabolomics indicated EE modulated gut microbiota and specifically increased the abundance of lithocholic acid(LCA),which might contribute to intestinal barrier function improvement via up-regulating the expression of colonic farnesoid X receptor(FXR).In summary,this study identified the anti-colitis mechanism of A.argyi EE by modulating gut microbiota,facilitating the production of LCA,activating FXR and improving intestinal barrier function.展开更多
but its application is constrained by hepatotoxicity.This study aimed to investigate the feasibility of reducing AAEO’s toxicity through storage and to evaluate changes in chemical composition,toxicity,and bioactivit...but its application is constrained by hepatotoxicity.This study aimed to investigate the feasibility of reducing AAEO’s toxicity through storage and to evaluate changes in chemical composition,toxicity,and bioactivity.Methods:Gas chromatography-mass spectrometry was used to analyze compositional changes during storage.Zebrafish acute toxicity tests and the liver-specific transgenic zebrafish model Tg(fabp10:EGFP)were used to assess toxicity.Antimicrobial,analgesic,and antioxidant assays evaluated variations in bioactivity.Results:Over the 150-day storage period,gas chromatography-mass spectrometry analysis identified 39 components.Zebrafish acute toxicity tests showed that the LD50 of AAEO stored for 0,30,60,90,120,and 150 days were 0.10μL·mL^(-1),0.10μL·mL^(-1),0.10μL·mL^(-1),0.11μL·mL^(-1),0.13μL·mL^(-1),and 0.14μL·mL^(-1),respectively,demonstrating a 40%reduction in acute toxicity after 150 days of storage.Using the liver-specific green fluorescent transgenic Tg(fabp10:EGFP)zebrafish model,the inhibition rates of AAEO on hepatic fluorescence intensity were measured at 68.5%,43.5%,42.6%,37.8%,34.6%,and 31.9% at different time points,confirming reduced hepatotoxicity after storage.Additionally,the antioxidant and analgesic activities of AAEO were significantly enhanced(p<0.05)after storage,while the antibacterial activity decreased(p<0.05).Conclusions:After storage,AAEO significantly reduces hepatotoxicity,with a 40% decrease in acute toxicity after 150 days.Meanwhile,the antioxidant and analgesic activities of AAEO increase,while its antibacterial activity decreases after storage.展开更多
A study was conducted to determine the antimicrobial activities of essential oil from Artemisiae argyi leaves. The sample of the essential oil was analyzed by GC-MS. From 18 compounds representing the oils, Eucalypto...A study was conducted to determine the antimicrobial activities of essential oil from Artemisiae argyi leaves. The sample of the essential oil was analyzed by GC-MS. From 18 compounds representing the oils, Eucalyptole （18.42%）, Spathulenol （14.32）, 4-Methyl-1-（1-methylethyl）-3-cyclohexen-1-ol （3.10%）, 3-Carene （2.64%） appeared as the main components. The screening of antimicrobial activity of the essential oil was evaluated using agar diffusion and broth microdilution methods. Gram-positive bacterial were more sensitive than gram-negative bacterial of the 8 microorganisms, and Staphylococcus aureus ATCC 6538 showed the lowest MIC （0.3125%） and MBC （0.625%）. In the disc diffusion assay, Staphylococcus epidermidis ATCC 49134 and Bacillus subtilis ATCC 6633 showed obvious inhibitory activity. Survival curve showed that, 2MIC ofArtemisiae argyi essential oil had a lethal effect on Candida albicans within the first 1 h. Results presented here suggest that the essential oil of Artemisiae argyi leaves possesses antimicrobial properties, and provides scientific foundations for exploition ofArtemisiae argyi.展开更多
Chemical constituents of the leaves of Artemisia argyi were investigated. By using a variety of chromatographic techniques and spectroscopic methods, six compounds were isolated and identified as follows： clemaphenol...Chemical constituents of the leaves of Artemisia argyi were investigated. By using a variety of chromatographic techniques and spectroscopic methods, six compounds were isolated and identified as follows： clemaphenol A （1）, aurantiamide acetate （2）, camelliagenin A （3）, japonica acid （4）, labd-13（E）-ene-8α,15-diol （5）, and 313-acetoxy-20-oxo-21-nordammaran-23-oic acid （6）. Among these products, compounds 1 and 3-6 were obtained from the genus Artemisia for the first time and compound 2 was firstly reported from the species. Additionally, compound 5 displayed an inhibitory effect against the lipopolysaccharide （LPS）-induced nitric oxide （NO） production in BV-2 microglial cells with an IC50 value of 6.68 μM.展开更多
OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were...OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were randomly assigned(n = 10/group) to a control group(C) or one of three moxa smoke concentration groups(% opacity): low(L1; 0.4%), medium(M1; 2%), and high(H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium(PS)-injected group, M1+PS group, H1(15%)+PS group,and a positive control group(10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography(GC-HS).RESULTS: A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION: The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System(CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.展开更多
Artemisia argyi(A.argyi),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-rel...Artemisia argyi(A.argyi),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving properties.Despite its widespread use,scientific evidence validating the antifungal efficacy of A.argyi water extract(AAWE)against dermatophytes,particularly Trichophyton rubrum,Trichophyton mentagrophytes,and Microsporum gypseum,remains limited.This study aimed to substantiate the scientific basis of the folkloric use of A.argyi by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against dermatophytes.The results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte species.The subfraction AAWE6,isolated using D101 macroporous resin,emerged as the most potent subfraction.The minimum inhibitory concentrations(MICs)of AAWE6 against T.rubrum,M.gypseum,and T.mentagrophytes were 312.5,312.5,and 625μg·mL−1,respectively.Transmission electron microscopy(TEM)results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of T.rubrum,creating breaches(“small holes”),and disrupt the internal mitochondrial structure(“granary”).Furthermore,transcriptome data,quantitative real-time PCR(RT-qPCR),and biochemical assays corroborated the severe disruption of mitochondrial function,evidenced by inhibited tricarboxylic acid(TCA)cycle and energy metabolism.Additionally,chemical characterization and molecular docking analyses identified flavonoids,primarily eupatilin(131.16±4.52 mg·g^(−1))and jaceosidin(4.17±0.18 mg·g^(−1)),as the active components of AAWE6.In conclusion,the subfraction AAWE6 from A.argyi exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and function.This research validates the traditional use of A.argyi and provides scientific support for its antidermatophytic applications,as recognized in the Chinese patent(No.ZL202111161301.9).展开更多
Phytoremediation effectiveness and remediation costs are driving factors of this project. Full utilization of plant resources after their being used for phytoremediation is an unsolved problem. GC/MS technique was use...Phytoremediation effectiveness and remediation costs are driving factors of this project. Full utilization of plant resources after their being used for phytoremediation is an unsolved problem. GC/MS technique was used to investigate the volatiles of the flowers from Elsholtzia argyi (PFE1: Purple Flower Elsholtzia) and their variation (WFE: White Flower Elsholtzia),naturally growing in Pb/Zn mined area, and Elsholtzia argyi (PFE2: Purple Flower Elsholtzia), naturally growing in Jiuxi uncontaminated agriculture soil. Seventeen compounds constituting 86.88% of total essential oils were identified in PFE1, with 2,6-octadienoic acid,3,7-dimethyl-methyl ester being the main constituent (63.30%). Sixteen compounds accounting for 95.32% of total essential oils were identified in WFE, with caryophyllene being the main component (55.02%). Compared to PFE1, PFE2 contained lower level of 2,6-octadienoic acid,3,7-dimethyl-methyl ester (31.76%), which was the main constituent in the total essential oils of PFE2. Caryophyllene is the main ingredient of flavor. Elsholtzia ketone was identified in all the three Elsholtzia plants. It can be concluded that the selected Elsholtzia argyi plants can be exploited on their versatile uses as fragrances and antiseptics due to the perfume ingredient and antibacterial components existing in their essential oils.展开更多
In the present study, we aimed to investigate the effects of Artemisia argyi essential oil（AO） on pulmonary hypertension（PH） induced by monocrotaline（MCT） and to explore the underlying mechanism. A total of 80 S...In the present study, we aimed to investigate the effects of Artemisia argyi essential oil（AO） on pulmonary hypertension（PH） induced by monocrotaline（MCT） and to explore the underlying mechanism. A total of 80 Sprague-Dawley rats were randomly divided into four groups as follows： control group, model group, bosentan（0.1 g/kg） group and AO（0.1 g/kg） group. After 30 d of experiment, hemodynamic parameters, lung and right ventricle hypertrophy index were determined. HE and Immunohistochemistry staining of lungs were performed to detect the injuries and protein expressions. The results showed that levels of m PAP, m RVP, max RVP, w W, LI, RV and RVHI as well as the expressions of NF-κB p65 and α-SMA were increased, small pulmonary artery thickened, the cavity of the arteriole narrowed, and there was marked infiltration of inflammatory cells in lungs of rats receiving MCT compared with the normal group. After the administration of AO, the levels of m PAP and m RVP were significantly decreased, and the w W, LI, LV＋S and pulmonary arterial remodeling were markedly improved. The expression levels of NF-κB p65 and α-SMA were reduced by AO compared with the model group. Our results suggested that AO reduced the progression of PH induced by MCT through inhibiting the expressions of NF-κB p65 and α-SMA.展开更多
Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant con...Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.展开更多
The volatile oil of Artemisia argyi was extracted by ultrasonic assisted extraction, and the extraction rate of volatile oil was 0.68%. Thevolatile oil of A. argyi was emulsified with 1% Tween-80, and drug susceptibil...The volatile oil of Artemisia argyi was extracted by ultrasonic assisted extraction, and the extraction rate of volatile oil was 0.68%. Thevolatile oil of A. argyi was emulsified with 1% Tween-80, and drug susceptibility test was conducted with avian Escherichia coli. The results showedthat the volatile oil of A. argyi had antibacterial effect against avian E. coli, and the minimal inhibitory concentration was 50 mg/mL. Taking sixcommon antibiotics as the control, drug susceptibility test was conducted with volatile oil of A. argyi. The results showed that 10 strains of E. coliwere sensitive to the volatile oil of A. argyi, three of which had different degrees of resistance and one had the tendency of resistance.展开更多
Objective:To comprehensively review the literature relating to the use of Artemisiae Argyi Folium (AAF;the dried leaf of Artemisia argyi Lévl.et Vant.from the Asteraceae family),and qi ai (AAF grown in Qichun Cou...Objective:To comprehensively review the literature relating to the use of Artemisiae Argyi Folium (AAF;the dried leaf of Artemisia argyi Lévl.et Vant.from the Asteraceae family),and qi ai (AAF grown in Qichun County and the adjacent areas in Hubei Province,China) in the field of traditional Chinese medicine.Furthermore,this study aimed to give results of the contents determination of three bioactive components in qi ai and AAF.Methods:The literature was reviewed to acquire relevant information regarding the quality and application of AAF and qi ai.In addition,the contents of the bioactive components (essential oil,total flavonoids and tannins) were determined in 29 AAF samples collected from China and Korea using the methods in the Chinese pharmacopoeia (2015) or determined by our experiments.Results:The dried leaf of A.argyi Lévl.et Vant.from the Asteraceae family is a mainstream commodity of AAF.Qi ai is a commercial type of AAF well-known for its good quality,which has been recorded in Chinese ancient literature.Modern research has showed that more intense heat with stronger penetration was generated in moxibustion therapy when qi ai moxa stick was burnt compared with general moxa stick,which led to a better curative effect.Our research also showed that the contents of essential oil,total flavonoids and tannin in qi ai were significantly higher than those of general AAF samples.Conclusion:The superior quality of qi ai compared with general AAF may be due to relatively higher contents of bioactive components.This study provides basic information for further study and utilization of AAF and qi ai.展开更多
[Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific b...[Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific basis for comprehensive development and rational utilization of Artemisia argyi. [Methods]Firstly,HS-SPME was used to extract the volatile components from the leaves,seeds,and stems of seven-tip Artemisia argyi,five-tip Artemisia argyi,and golden Artemisia argyi. Their chemical composition was analyzed by gas chromatographymass spectrometry( GC-MS),and the relative mass fraction of each component was determined by area normalization method. [Results] For the same variety of Artemisia argyi,the content of volatile components in leaves was higher than in seeds and stems except camphor,and the content of volatile components in seven-tip Artemisia argyi leaves was higher than in five-tip Artemisia argyi and golden Artemisia argyi leaves.HS-SPME-GC-MS analysis showed that eucalyptol,camphor,thujone,α-pinene,1-caryophyllene and caryophyllene oxide with higher content were detected in different parts of the different cultivars of Artemisia argyi. [Conclusions]Seven-tip Artemisia argyi leaves were the best medicinal parts of the three cultivars,followed by seven-tip Artemisia argyi seeds.展开更多
[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the co...[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.展开更多
[Objectives] This study aimed to optimize the conditions for pressurized synchronous extraction of volatile oils, total flavonoids and tannic acid from Artemisia argyi .[Methods] Single factor experiments and orthogon...[Objectives] This study aimed to optimize the conditions for pressurized synchronous extraction of volatile oils, total flavonoids and tannic acid from Artemisia argyi .[Methods] Single factor experiments and orthogonal experiments were conducted.[Results] Pressurization had a significant effect on the extraction rate of chemical constituents of A. argyi . The optimal conditions for pressurized synchronous extraction of volatile oils, total flavonoids and tannic acid from A. argyi were as follows: solvent concentration of 70%, solid to liquid ratio of 1∶ 30 (g/mL), extraction pressure of 0.9 MPa, extraction time of 40 min and extraction temperature of 90 ℃. Under the optimal extraction conditions, the yields of volatile oils, total flavonoids and tannic acid were 0.852%, 4.66% and 6.79%, respectively.[Conclusions] Compared with other extraction methods, the pressurized assistant solvent extraction method can achieve the synchronous extraction of volatile oils, total flavonoids and tannic acid from A. argyi . The process is stable, short in extraction time, high in solvent utilization rate and ideal in extraction effect of the three components from A. argyi .展开更多
[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield o...[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield of Artemisia argyi leaf in Qichun(AALQ).[Methods]The content of the total flavonoids in AALQ was determined by UV-Vis spectrophotometry with apigenin as reference substance.The contents of volatile oil and tannins were determined by the methods in Chinese Pharmacopoeia(2015 edition).The moxa yield was detected by the self-developed method of grinding and sifting with high-speed pulverizer.[Results]Whether applying cake fertilizer,or compound fertilizer after applying barnyard manure,the contents of total flavonoids and tannins in AALQ were reduced in varying degrees,and the content of volatile oil was changed,which had adversely affect the accumulation of effective substances in the leaves of medicinal A.argyi as a whole.The contents of related chemical substances and moxa yield of A.argyi planted in the neighbouring Xishui County from Qichun,Hubei Province were relatively high.[Conclusions]The contents of effective substances and moxa yield of AALQ were significantly reduced as a whole when applied fertilizer in planting,especially after the application of barnyard manure,compound fertilizer was added.The quality of A.argyi leaf would not be adversely affected if it was transplanted from Qichun County,Hubei Province,which is the genuine producing area,to nearby areas with similar ecological environment.展开更多
As a traditional herbal medicine for the treatment of many disorders, Artemisia argyi has shown many valuable bioactivities, but little is known about its effect on Staphylococcus aureus. In this study, the growth, th...As a traditional herbal medicine for the treatment of many disorders, Artemisia argyi has shown many valuable bioactivities, but little is known about its effect on Staphylococcus aureus. In this study, the growth, the biofilm formation and the pathogenicity of S. aureus cultivated with or without ethanol extract of A. argyi were tested using microtitre plate assay, Confocal Laser Scanning Microscope (CLSM) system and mice infection assay. Results showed that the growth and the biofilm formation of S. aureus in the test group with ethanol extract of A. argyi were significantly lower than those of the control group without ethanol extract of A. argyi. With CLSM system we could observe that the biofilm structure of the test group had looser and less biomass compared with the control group. After infection of S. aureus, the survival of mice in test group that were given 0.2 mL 100 mg/mL ethanol extracts of A. argyi was higher than the control group. Histopathological analyses showed that the tissue damage of mice in test group was less than that in control group. These results suggested that ethanol extract of A. argyi had inhibitory effect on S. aureus and could protect mice from death induced by S. aureus infection.展开更多
Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s heal...Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s health awareness,experts and scholars are concerned about the nutrients and effects of A.argyi.A.argyi is rich in carbohydrates,proteins,essential amino acids,fatty acids,minerals,vitamins,dietary fibers,flavonoids and other nutrients.It has the effects of antioxidant,anti-tumor,antibacterial,antiinflammatory,neuroprotection and immune regulations,and has been widely applied in the fields of COVID-19 prevention,food health care,food preservation and feed breeding,and has good development potential.This review summarized the research and application of A.argyi nutrients,in order to provide reference and application for further study.展开更多
Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-d...Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-depth research on the chemical composition and pharmacology.It is mainly used clinically for the treatment of“cold pain in the abdomen,cold clotting in the menstrual channels,cold infertility in the uterus,fetal movement,vomiting blood,epistaxis,pus and blood dysentery,and external treatment of itchy skin”and other symptoms.This paper reviews the research on the biological activity and chemical composition of A.argyi by scholars in recent decades.展开更多
基金financially supported by the Natural Science Foundation of Zhejiang Province(LY22C010002)China Postdoctoral Science Foundation(2022M721732)+1 种基金the project of sending sci-tech experts to rural areas in Ningbo city(2022S205)the K.C.Wong Magna Fund of Ningbo University.
文摘Artemisia argyi(A.argyi)is a Chinese herbal medicine with reported anti-inflammatory effects.In this study,the A.argyi was extracted with water and ethanol,and the concentrations of 35 flavonoids in A.argyi water extract(WE)and ethanol extract(EE)were measured via targeted metabolomics.The antioxidant and antiinflammatory activities of both WE and EE were firstly explored in vitro via chemical assays and cellular experiment,respectively.Both WE and EE showed significant 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),·OH,and O_(2)·radical scavenging ability in a dose-dependent manner,and reduced the levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-22(IL-22)in lipopolysaccharide(LPS)induced RAW264.7 cell model.In addition,the in vivo anti-colitis activity of both extracts was investigated in dextran sulfate sodium(DSS)-induced colitis mice,and the underlying mechanisms were elucidated by 16S r DNA sequencing and targeted metabolomics.We found that both WE and EE relieved colitis in mice,characterized by decreased disease activity index,increased colon length,improved pathological changes in colon tissue,while EE showed better anti-colitis activity.In addition,both 16S r DNA sequencing and targeted bile acids metabolomics indicated EE modulated gut microbiota and specifically increased the abundance of lithocholic acid(LCA),which might contribute to intestinal barrier function improvement via up-regulating the expression of colonic farnesoid X receptor(FXR).In summary,this study identified the anti-colitis mechanism of A.argyi EE by modulating gut microbiota,facilitating the production of LCA,activating FXR and improving intestinal barrier function.
基金supported by grants from the National Natural Science Foundation of China(82074026)the National Interdisciplinary Innovation Team of Traditional Chinese Medicine(ZYYCXTD-D-202209).
文摘but its application is constrained by hepatotoxicity.This study aimed to investigate the feasibility of reducing AAEO’s toxicity through storage and to evaluate changes in chemical composition,toxicity,and bioactivity.Methods:Gas chromatography-mass spectrometry was used to analyze compositional changes during storage.Zebrafish acute toxicity tests and the liver-specific transgenic zebrafish model Tg(fabp10:EGFP)were used to assess toxicity.Antimicrobial,analgesic,and antioxidant assays evaluated variations in bioactivity.Results:Over the 150-day storage period,gas chromatography-mass spectrometry analysis identified 39 components.Zebrafish acute toxicity tests showed that the LD50 of AAEO stored for 0,30,60,90,120,and 150 days were 0.10μL·mL^(-1),0.10μL·mL^(-1),0.10μL·mL^(-1),0.11μL·mL^(-1),0.13μL·mL^(-1),and 0.14μL·mL^(-1),respectively,demonstrating a 40%reduction in acute toxicity after 150 days of storage.Using the liver-specific green fluorescent transgenic Tg(fabp10:EGFP)zebrafish model,the inhibition rates of AAEO on hepatic fluorescence intensity were measured at 68.5%,43.5%,42.6%,37.8%,34.6%,and 31.9% at different time points,confirming reduced hepatotoxicity after storage.Additionally,the antioxidant and analgesic activities of AAEO were significantly enhanced(p<0.05)after storage,while the antibacterial activity decreased(p<0.05).Conclusions:After storage,AAEO significantly reduces hepatotoxicity,with a 40% decrease in acute toxicity after 150 days.Meanwhile,the antioxidant and analgesic activities of AAEO increase,while its antibacterial activity decreases after storage.
文摘A study was conducted to determine the antimicrobial activities of essential oil from Artemisiae argyi leaves. The sample of the essential oil was analyzed by GC-MS. From 18 compounds representing the oils, Eucalyptole （18.42%）, Spathulenol （14.32）, 4-Methyl-1-（1-methylethyl）-3-cyclohexen-1-ol （3.10%）, 3-Carene （2.64%） appeared as the main components. The screening of antimicrobial activity of the essential oil was evaluated using agar diffusion and broth microdilution methods. Gram-positive bacterial were more sensitive than gram-negative bacterial of the 8 microorganisms, and Staphylococcus aureus ATCC 6538 showed the lowest MIC （0.3125%） and MBC （0.625%）. In the disc diffusion assay, Staphylococcus epidermidis ATCC 49134 and Bacillus subtilis ATCC 6633 showed obvious inhibitory activity. Survival curve showed that, 2MIC ofArtemisiae argyi essential oil had a lethal effect on Candida albicans within the first 1 h. Results presented here suggest that the essential oil of Artemisiae argyi leaves possesses antimicrobial properties, and provides scientific foundations for exploition ofArtemisiae argyi.
基金National Natural Science Foundation of China(Grant No. 30973629)
文摘Chemical constituents of the leaves of Artemisia argyi were investigated. By using a variety of chromatographic techniques and spectroscopic methods, six compounds were isolated and identified as follows： clemaphenol A （1）, aurantiamide acetate （2）, camelliagenin A （3）, japonica acid （4）, labd-13（E）-ene-8α,15-diol （5）, and 313-acetoxy-20-oxo-21-nordammaran-23-oic acid （6）. Among these products, compounds 1 and 3-6 were obtained from the genus Artemisia for the first time and compound 2 was firstly reported from the species. Additionally, compound 5 displayed an inhibitory effect against the lipopolysaccharide （LPS）-induced nitric oxide （NO） production in BV-2 microglial cells with an IC50 value of 6.68 μM.
基金Supported by the National Natural Science Foundation of China:Effect of Moxibustion on"Longevity Gene"SIRT1 Regulation in Aged Mice(No.81403449)NSFC Project:Effect of Moxibustion and its Therapeutic Factors on Mitochondrial Energy Metabolism and Apotosis Pathyway in APP/PS1Transgenic Alzheimer's Disease Mouse Model(No.81574068)National Program on Key Basic Research Project(973 Program):the Study of Components and Mechanisms of Artemisia and the Product and Moxibustion and Safety Evaluation(No.2009CB522906)
文摘OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were randomly assigned(n = 10/group) to a control group(C) or one of three moxa smoke concentration groups(% opacity): low(L1; 0.4%), medium(M1; 2%), and high(H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium(PS)-injected group, M1+PS group, H1(15%)+PS group,and a positive control group(10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography(GC-HS).RESULTS: A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION: The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System(CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.
基金This work was supported by the National Natural Science Foundation of China(No.32270391)the Natural Science Foundation of Hubei Province(Nos.2023AFA032 and 2022CFB391)+1 种基金the Young Qihuang Scholars of the State Administration of Traditional Chinese Medicine,Hubei Province Administration of Traditional Chinese Medicine Research Project(No.ZY2023Z023)the Earmarked Fund for CARS-21 and Key Project at Central Government Level:the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302).
文摘Artemisia argyi(A.argyi),a plant with a longstanding history as a raw material for traditional medicine and functional diets in Asia,has been used traditionally to bathe and soak feet for its disinfectant and itch-relieving properties.Despite its widespread use,scientific evidence validating the antifungal efficacy of A.argyi water extract(AAWE)against dermatophytes,particularly Trichophyton rubrum,Trichophyton mentagrophytes,and Microsporum gypseum,remains limited.This study aimed to substantiate the scientific basis of the folkloric use of A.argyi by evaluating the antifungal effects and the underlying molecular mechanisms of its active subfraction against dermatophytes.The results indicated that AAWE exhibited excellent antifungal effects against the three aforementioned dermatophyte species.The subfraction AAWE6,isolated using D101 macroporous resin,emerged as the most potent subfraction.The minimum inhibitory concentrations(MICs)of AAWE6 against T.rubrum,M.gypseum,and T.mentagrophytes were 312.5,312.5,and 625μg·mL−1,respectively.Transmission electron microscopy(TEM)results and assays of enzymes linked to cell wall integrity and cell membrane function indicated that AAWE6 could penetrate the external protective barrier of T.rubrum,creating breaches(“small holes”),and disrupt the internal mitochondrial structure(“granary”).Furthermore,transcriptome data,quantitative real-time PCR(RT-qPCR),and biochemical assays corroborated the severe disruption of mitochondrial function,evidenced by inhibited tricarboxylic acid(TCA)cycle and energy metabolism.Additionally,chemical characterization and molecular docking analyses identified flavonoids,primarily eupatilin(131.16±4.52 mg·g^(−1))and jaceosidin(4.17±0.18 mg·g^(−1)),as the active components of AAWE6.In conclusion,the subfraction AAWE6 from A.argyi exerts antifungal effects against dermatophytes by disrupting mitochondrial morphology and function.This research validates the traditional use of A.argyi and provides scientific support for its antidermatophytic applications,as recognized in the Chinese patent(No.ZL202111161301.9).
文摘Phytoremediation effectiveness and remediation costs are driving factors of this project. Full utilization of plant resources after their being used for phytoremediation is an unsolved problem. GC/MS technique was used to investigate the volatiles of the flowers from Elsholtzia argyi (PFE1: Purple Flower Elsholtzia) and their variation (WFE: White Flower Elsholtzia),naturally growing in Pb/Zn mined area, and Elsholtzia argyi (PFE2: Purple Flower Elsholtzia), naturally growing in Jiuxi uncontaminated agriculture soil. Seventeen compounds constituting 86.88% of total essential oils were identified in PFE1, with 2,6-octadienoic acid,3,7-dimethyl-methyl ester being the main constituent (63.30%). Sixteen compounds accounting for 95.32% of total essential oils were identified in WFE, with caryophyllene being the main component (55.02%). Compared to PFE1, PFE2 contained lower level of 2,6-octadienoic acid,3,7-dimethyl-methyl ester (31.76%), which was the main constituent in the total essential oils of PFE2. Caryophyllene is the main ingredient of flavor. Elsholtzia ketone was identified in all the three Elsholtzia plants. It can be concluded that the selected Elsholtzia argyi plants can be exploited on their versatile uses as fragrances and antiseptics due to the perfume ingredient and antibacterial components existing in their essential oils.
基金The Natural Science Foundation of Zhejiang Province of China(Grant No.LQ14H280003)the TCM Project of Zhejiang Province(Grant No.2011ZA006)+2 种基金the Chinese Integrative Medicine Foundation of Zhejiang Province(Grant No.2013LYSX020)Zhejiang Provincial Program for the Cultivation of High-level Innovative Heath Talents(Ping Huang)Zhejiang 151 Elites Project,the second level(Ping Huang)
文摘In the present study, we aimed to investigate the effects of Artemisia argyi essential oil（AO） on pulmonary hypertension（PH） induced by monocrotaline（MCT） and to explore the underlying mechanism. A total of 80 Sprague-Dawley rats were randomly divided into four groups as follows： control group, model group, bosentan（0.1 g/kg） group and AO（0.1 g/kg） group. After 30 d of experiment, hemodynamic parameters, lung and right ventricle hypertrophy index were determined. HE and Immunohistochemistry staining of lungs were performed to detect the injuries and protein expressions. The results showed that levels of m PAP, m RVP, max RVP, w W, LI, RV and RVHI as well as the expressions of NF-κB p65 and α-SMA were increased, small pulmonary artery thickened, the cavity of the arteriole narrowed, and there was marked infiltration of inflammatory cells in lungs of rats receiving MCT compared with the normal group. After the administration of AO, the levels of m PAP and m RVP were significantly decreased, and the w W, LI, LV＋S and pulmonary arterial remodeling were markedly improved. The expression levels of NF-κB p65 and α-SMA were reduced by AO compared with the model group. Our results suggested that AO reduced the progression of PH induced by MCT through inhibiting the expressions of NF-κB p65 and α-SMA.
文摘Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.
基金Supported by Scientific Research and Technology Development Program of Guangxi ProvinceConstruction of Science and Technology Service Platform in Hezhou Agricultural Science and Tech-nology Park(GKN 14258003)Scientific Research Project of Hezhou University(HZU-JS201617)
文摘The volatile oil of Artemisia argyi was extracted by ultrasonic assisted extraction, and the extraction rate of volatile oil was 0.68%. Thevolatile oil of A. argyi was emulsified with 1% Tween-80, and drug susceptibility test was conducted with avian Escherichia coli. The results showedthat the volatile oil of A. argyi had antibacterial effect against avian E. coli, and the minimal inhibitory concentration was 50 mg/mL. Taking sixcommon antibiotics as the control, drug susceptibility test was conducted with volatile oil of A. argyi. The results showed that 10 strains of E. coliwere sensitive to the volatile oil of A. argyi, three of which had different degrees of resistance and one had the tendency of resistance.
文摘Objective:To comprehensively review the literature relating to the use of Artemisiae Argyi Folium (AAF;the dried leaf of Artemisia argyi Lévl.et Vant.from the Asteraceae family),and qi ai (AAF grown in Qichun County and the adjacent areas in Hubei Province,China) in the field of traditional Chinese medicine.Furthermore,this study aimed to give results of the contents determination of three bioactive components in qi ai and AAF.Methods:The literature was reviewed to acquire relevant information regarding the quality and application of AAF and qi ai.In addition,the contents of the bioactive components (essential oil,total flavonoids and tannins) were determined in 29 AAF samples collected from China and Korea using the methods in the Chinese pharmacopoeia (2015) or determined by our experiments.Results:The dried leaf of A.argyi Lévl.et Vant.from the Asteraceae family is a mainstream commodity of AAF.Qi ai is a commercial type of AAF well-known for its good quality,which has been recorded in Chinese ancient literature.Modern research has showed that more intense heat with stronger penetration was generated in moxibustion therapy when qi ai moxa stick was burnt compared with general moxa stick,which led to a better curative effect.Our research also showed that the contents of essential oil,total flavonoids and tannin in qi ai were significantly higher than those of general AAF samples.Conclusion:The superior quality of qi ai compared with general AAF may be due to relatively higher contents of bioactive components.This study provides basic information for further study and utilization of AAF and qi ai.
基金Supported by Natural Science Foundation of Hubei Province(2004AB242)
文摘[Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific basis for comprehensive development and rational utilization of Artemisia argyi. [Methods]Firstly,HS-SPME was used to extract the volatile components from the leaves,seeds,and stems of seven-tip Artemisia argyi,five-tip Artemisia argyi,and golden Artemisia argyi. Their chemical composition was analyzed by gas chromatographymass spectrometry( GC-MS),and the relative mass fraction of each component was determined by area normalization method. [Results] For the same variety of Artemisia argyi,the content of volatile components in leaves was higher than in seeds and stems except camphor,and the content of volatile components in seven-tip Artemisia argyi leaves was higher than in five-tip Artemisia argyi and golden Artemisia argyi leaves.HS-SPME-GC-MS analysis showed that eucalyptol,camphor,thujone,α-pinene,1-caryophyllene and caryophyllene oxide with higher content were detected in different parts of the different cultivars of Artemisia argyi. [Conclusions]Seven-tip Artemisia argyi leaves were the best medicinal parts of the three cultivars,followed by seven-tip Artemisia argyi seeds.
文摘[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.
基金Supported by Guiding Science and Technology Plan Project of Yongzhou City(Yong Ke Fa[2015]10)
文摘[Objectives] This study aimed to optimize the conditions for pressurized synchronous extraction of volatile oils, total flavonoids and tannic acid from Artemisia argyi .[Methods] Single factor experiments and orthogonal experiments were conducted.[Results] Pressurization had a significant effect on the extraction rate of chemical constituents of A. argyi . The optimal conditions for pressurized synchronous extraction of volatile oils, total flavonoids and tannic acid from A. argyi were as follows: solvent concentration of 70%, solid to liquid ratio of 1∶ 30 (g/mL), extraction pressure of 0.9 MPa, extraction time of 40 min and extraction temperature of 90 ℃. Under the optimal extraction conditions, the yields of volatile oils, total flavonoids and tannic acid were 0.852%, 4.66% and 6.79%, respectively.[Conclusions] Compared with other extraction methods, the pressurized assistant solvent extraction method can achieve the synchronous extraction of volatile oils, total flavonoids and tannic acid from A. argyi . The process is stable, short in extraction time, high in solvent utilization rate and ideal in extraction effect of the three components from A. argyi .
文摘[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield of Artemisia argyi leaf in Qichun(AALQ).[Methods]The content of the total flavonoids in AALQ was determined by UV-Vis spectrophotometry with apigenin as reference substance.The contents of volatile oil and tannins were determined by the methods in Chinese Pharmacopoeia(2015 edition).The moxa yield was detected by the self-developed method of grinding and sifting with high-speed pulverizer.[Results]Whether applying cake fertilizer,or compound fertilizer after applying barnyard manure,the contents of total flavonoids and tannins in AALQ were reduced in varying degrees,and the content of volatile oil was changed,which had adversely affect the accumulation of effective substances in the leaves of medicinal A.argyi as a whole.The contents of related chemical substances and moxa yield of A.argyi planted in the neighbouring Xishui County from Qichun,Hubei Province were relatively high.[Conclusions]The contents of effective substances and moxa yield of AALQ were significantly reduced as a whole when applied fertilizer in planting,especially after the application of barnyard manure,compound fertilizer was added.The quality of A.argyi leaf would not be adversely affected if it was transplanted from Qichun County,Hubei Province,which is the genuine producing area,to nearby areas with similar ecological environment.
文摘As a traditional herbal medicine for the treatment of many disorders, Artemisia argyi has shown many valuable bioactivities, but little is known about its effect on Staphylococcus aureus. In this study, the growth, the biofilm formation and the pathogenicity of S. aureus cultivated with or without ethanol extract of A. argyi were tested using microtitre plate assay, Confocal Laser Scanning Microscope (CLSM) system and mice infection assay. Results showed that the growth and the biofilm formation of S. aureus in the test group with ethanol extract of A. argyi were significantly lower than those of the control group without ethanol extract of A. argyi. With CLSM system we could observe that the biofilm structure of the test group had looser and less biomass compared with the control group. After infection of S. aureus, the survival of mice in test group that were given 0.2 mL 100 mg/mL ethanol extracts of A. argyi was higher than the control group. Histopathological analyses showed that the tissue damage of mice in test group was less than that in control group. These results suggested that ethanol extract of A. argyi had inhibitory effect on S. aureus and could protect mice from death induced by S. aureus infection.
基金Supported by the Research on Scientific and Technological Innovation of Beef Cattle in Northeast Cold Region by Ministry of Education(002020010)。
文摘Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s health awareness,experts and scholars are concerned about the nutrients and effects of A.argyi.A.argyi is rich in carbohydrates,proteins,essential amino acids,fatty acids,minerals,vitamins,dietary fibers,flavonoids and other nutrients.It has the effects of antioxidant,anti-tumor,antibacterial,antiinflammatory,neuroprotection and immune regulations,and has been widely applied in the fields of COVID-19 prevention,food health care,food preservation and feed breeding,and has good development potential.This review summarized the research and application of A.argyi nutrients,in order to provide reference and application for further study.
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-depth research on the chemical composition and pharmacology.It is mainly used clinically for the treatment of“cold pain in the abdomen,cold clotting in the menstrual channels,cold infertility in the uterus,fetal movement,vomiting blood,epistaxis,pus and blood dysentery,and external treatment of itchy skin”and other symptoms.This paper reviews the research on the biological activity and chemical composition of A.argyi by scholars in recent decades.
