[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Meth...[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.展开更多
Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions...Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.展开更多
[Objectives]This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No.1 as the test material.[Methods]Huanyou No.1 was sprayed with chitin,and the changes of some p...[Objectives]This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No.1 as the test material.[Methods]Huanyou No.1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated.[Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose;and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme andβ-Gal enzyme.[Conclusions]Chitin spray treatment could increase the yield of A.arguta,and improve its quality and storage property.展开更多
[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid rat...[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.展开更多
Actinidia arguta is a newly emerged,commercially cultivated Actinidia species.A.arguta has a beautiful appearance and is rich in anthocyanin,and is thus highly welcomed by consumers.However,the mechanism of anthocyani...Actinidia arguta is a newly emerged,commercially cultivated Actinidia species.A.arguta has a beautiful appearance and is rich in anthocyanin,and is thus highly welcomed by consumers.However,the mechanism of anthocyanin regulation in A.arguta remains unclear.In this study,we assembled the nearly complete genome of the first red A.arguta cultivar,‘Tianyuanhong’,with an N50 of 21 Mb.Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A.arguta.Through verification of transient overexpression and stable transformation,RNA-seq analysis revealed a key bHLH transcription factor,AaBEE1,which negatively regulates anthocyanin biosynthesis.DAP-seq analysis combined with Y1H,EMSA,Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression.Overall,we assembled the genome of A.arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.展开更多
Actinidia arguta fruit is famous for its nutritional value,and we can eat the whole fruit with seeds.However,the nutrition of the seeds has yet to be systematically studied,while the value of seeds as by-products of p...Actinidia arguta fruit is famous for its nutritional value,and we can eat the whole fruit with seeds.However,the nutrition of the seeds has yet to be systematically studied,while the value of seeds as by-products of processed products is worth exploring.besides,the nutritional composition in distinct germplasms has not been reported.in this study,the nutritional indexes of 39 germplasms A.arguta seeds,including fatty acids,amino acids,minerals,phenolic,flavonoids,and starch had been compared and the correlations of these nutrition also had been explored.The results provided that A.arguta seeds were rich in nutrition.Eight fatty acids were found and the ω-6/ω-3 ratio was 0.07 to 2.01.The total amino acid contents ranged from 9.25 to 29.45 mg/100 g.The largest major mineral element was magnesium(216.62-42117.22 mg/kg),and the largest trace mineral element was iron(13.05-1326.48 mg/kg).The phenolic,flavonoids,and starch contents were 1.33−5.82 mg/g,0.41−0.87 mg/g,11.29%-31.93%,respectively.There were correlations among nutritional indexes,which had significant differences in 39 germplasms of A.arguta seeds.in order to assess comprehensive nutrition,using the weighted average score system and one germplasm,CJ No.1,with superior nutritional quality,was identified.The results of this study will enrich the research content of A.arguta seed,provide theoretical guidance for A.arguta seed to become a dietary supplement,reduce the production and processing waste,and promote the development of A.arguta processing industry.展开更多
Actinidia arguta is a type of kiwi fruit with a smooth glabrous shape,which provides high nutritional value,a sweet and sour taste,and excellent healthcare function.However,it is a typical climacteric fruit and decomp...Actinidia arguta is a type of kiwi fruit with a smooth glabrous shape,which provides high nutritional value,a sweet and sour taste,and excellent healthcare function.However,it is a typical climacteric fruit and decomposes quickly when stored.For delaying senescence,the effect of postharvest short-time partial dehydration(STPD)on the storage quality of A.arguta was investigated under dehydration rates of 2%,4%,and 6%coupled with storage at(2.0±0.5)℃,respectively.Results showed that appropriate dehydration treatment coupled with low temperature storage inhibited the pectinase activity and the decomposition of propectin,thus maintaining fruit firmness,reducing fruit decay rate,regulating the generation of abscisic acid delaying ethylene production,and decreasing the peak level of respiration rate.it also inhibited the reduction of total soluble solid,titratable acid,and vitamin C,maintains catalase and peroxidase activity.based on the present study,the 4%dehydration treatment was the most effective,which extended the storage period up to 60 days and reduced the decay rate to 33%.in conclusion,it was revealed that STPD could be an effective method for retarding the losses that occurred in the fruit quality of A.arguta during cold storage.展开更多
【目的】分析国内外软枣猕猴桃的研究现状,以期为中国软枣猕猴桃的可持续发展提供建议。【方法】以中国知网(CNKI)1979—2024年和WOS(Web of Science)1966—2024年数据库中软枣猕猴桃学术论文为研究对象,运用CiteSpace、VOSviewer进行...【目的】分析国内外软枣猕猴桃的研究现状,以期为中国软枣猕猴桃的可持续发展提供建议。【方法】以中国知网(CNKI)1979—2024年和WOS(Web of Science)1966—2024年数据库中软枣猕猴桃学术论文为研究对象,运用CiteSpace、VOSviewer进行文献计量学分析。【结果】统计年限期间,关于软枣猕猴桃的文献共发表论文1283篇(中文823篇,英文460篇);中英文发文量分别从2010年和2016年开始快速上升,且中文的文献数量多于英文;中国在发文机构、作者和期刊数量等方面占据优势;国内主要关注资源调查、品种选育、组织培养、栽培技术、果实品质、功能性等研究,国外倾向于研究软枣猕猴桃功能性、基因表达、贮藏性能、品种选育、组织培养等方面。然而,中外在软枣猕猴桃的植物保护等方面的研究均较少,亟须进一步加强。【结论】中国在软枣猕猴桃研究上中、英文发文量占总文献量的75.60%,具有明显优势,研究主要集中在资源与育种、栽培技术、果实品质与功能等领域;国外则偏重于功能性、基因表达、贮藏性能等研究,应加强基础研究与应用研究、产业发展的融合。展开更多
The fruit of Actinidia arguta is typically climacteric.During ripening,the fruits produce a large amount of ethylene.Although the molecular basis of climacteric fruit ripening has been extensively studied,some aspects...The fruit of Actinidia arguta is typically climacteric.During ripening,the fruits produce a large amount of ethylene.Although the molecular basis of climacteric fruit ripening has been extensively studied,some aspects such as its transcriptional regulation remain unclear.Here,we compared the transcriptomes of A.arguta fruits collected at commercial harvest(sample d0)and at 6 days after harvest(sample d6)using RNA-seq.A total of 3 659 differentially expressed genes(DEGs)between samples d0 and d6 were detected,of which 2 983 and 1 104 could be functionally annotated with Gene Ontology(GO)and Clusters of Orthologous Groups(COG)pathways.DEGs involved in ethylene biosynthesis and signal transduction were identified,including ACO(c79627),ERF061(c105131),and ERF6(c99193).Transcription factors such as ERF,b HLH,NAC,MADS,and MYB were also differentially expressed between samples d0 and d6.Selected DEGs were subjected to further analysis using quantitative reverse transcription-polymerase chain reaction(q RT-PCR),and the results coincided with those of RNA-seq.Our data revealed additional transcription factors that are involved in the regulation of fruit ripening.These results provide useful information for future research on the transcriptional regulation of fruit ripening.展开更多
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303093)the Key Project for Agricultural Improved Variety Breeding of Shandong Province (Lunongliang[2016]No.6~~
文摘[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.
基金supported by Special Scientific Research Fund of Agricultural Public Welfare Profession of China[grant number 200903013]Liaoning Province,Shenyang Agricultural University,high-end talent introduction fund project[grant number SYAU20160003]。
文摘Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.
文摘[Objectives]This study was conducted to discuss the effect of chitin treatment on softening of Actinidia arguta Huanyou No.1 as the test material.[Methods]Huanyou No.1 was sprayed with chitin,and the changes of some physiological and biochemical indexes in fruit softening were investigated.[Results]Chitin treatment significantly improved single fruit weight,increased soluble solid content,but decreased titratable acid content and significantly reduced the content of cellulose and hemicellulose;and the chitin treatment significantly inhibited the breakdown of pectin,cellulose and hemicellulose,and inhibited the activities of PG enzyme andβ-Gal enzyme.[Conclusions]Chitin spray treatment could increase the yield of A.arguta,and improve its quality and storage property.
基金National Undergraduate Innovation and Entrepreneurship Training Program of China(202111430049).
文摘[Objectives]To optimize the extraction process of polysaccharides from Actinidia arguta(Sieb.&Zucc.)Planch.ex Miq.[Methods]On the basis of single-factor tests,an orthogonal test was conducted with solid/liquid ratio,ultrasonic temperature,ultrasonic time and ultrasonic power as influencing factors.[Results]The optimal extraction process was as follows:solid/liquid ratio 1∶15(g/mL),ultrasonic temperature 50℃,ultrasonic time 50 min and ultrasonic power 90 W.Under the optimal extraction conditions,the extraction rate of polysaccharides reached 2.05%.[Conclusions]The method is simple and feasible,and can be used to optimize the extraction process of polysaccharides in A.arguta,in order to provide a theoretical basis for the further development and utilization of related resources.
基金supported by grants from National Key Research and Development Program of China(2022YFD1200503)National Natural Science Foundation of China(32202435)+4 种基金China Agriculture Research System of MOF and MARA(CARS-26)National Key Research and Development Program of China(2022YFD1600700)Major Science and Technology Projects of Henan Province(221100110400)Technical System of Bulk Fruit Industry in Henan Province(HARS-22-09-S)Agricultural Science and Technology Innovation Program,and Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2023-ZFRI-03).
文摘Actinidia arguta is a newly emerged,commercially cultivated Actinidia species.A.arguta has a beautiful appearance and is rich in anthocyanin,and is thus highly welcomed by consumers.However,the mechanism of anthocyanin regulation in A.arguta remains unclear.In this study,we assembled the nearly complete genome of the first red A.arguta cultivar,‘Tianyuanhong’,with an N50 of 21 Mb.Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A.arguta.Through verification of transient overexpression and stable transformation,RNA-seq analysis revealed a key bHLH transcription factor,AaBEE1,which negatively regulates anthocyanin biosynthesis.DAP-seq analysis combined with Y1H,EMSA,Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression.Overall,we assembled the genome of A.arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.
基金supported by Liaoning Provincial CentralGuiding Local Science and Technology Development Fund Project(20231H6/100100003)Shenyang Science and Technology Planseed Industry Innovation Project(22-318-2-11).
文摘Actinidia arguta fruit is famous for its nutritional value,and we can eat the whole fruit with seeds.However,the nutrition of the seeds has yet to be systematically studied,while the value of seeds as by-products of processed products is worth exploring.besides,the nutritional composition in distinct germplasms has not been reported.in this study,the nutritional indexes of 39 germplasms A.arguta seeds,including fatty acids,amino acids,minerals,phenolic,flavonoids,and starch had been compared and the correlations of these nutrition also had been explored.The results provided that A.arguta seeds were rich in nutrition.Eight fatty acids were found and the ω-6/ω-3 ratio was 0.07 to 2.01.The total amino acid contents ranged from 9.25 to 29.45 mg/100 g.The largest major mineral element was magnesium(216.62-42117.22 mg/kg),and the largest trace mineral element was iron(13.05-1326.48 mg/kg).The phenolic,flavonoids,and starch contents were 1.33−5.82 mg/g,0.41−0.87 mg/g,11.29%-31.93%,respectively.There were correlations among nutritional indexes,which had significant differences in 39 germplasms of A.arguta seeds.in order to assess comprehensive nutrition,using the weighted average score system and one germplasm,CJ No.1,with superior nutritional quality,was identified.The results of this study will enrich the research content of A.arguta seed,provide theoretical guidance for A.arguta seed to become a dietary supplement,reduce the production and processing waste,and promote the development of A.arguta processing industry.
基金supported by Liaoning Provincial Central Guiding Local Science and Technology Development Fund Project(2023JH6/100100003)Shenyang Science and Technology Plan Seed industry innovation Project(22-318-2-11).
文摘Actinidia arguta is a type of kiwi fruit with a smooth glabrous shape,which provides high nutritional value,a sweet and sour taste,and excellent healthcare function.However,it is a typical climacteric fruit and decomposes quickly when stored.For delaying senescence,the effect of postharvest short-time partial dehydration(STPD)on the storage quality of A.arguta was investigated under dehydration rates of 2%,4%,and 6%coupled with storage at(2.0±0.5)℃,respectively.Results showed that appropriate dehydration treatment coupled with low temperature storage inhibited the pectinase activity and the decomposition of propectin,thus maintaining fruit firmness,reducing fruit decay rate,regulating the generation of abscisic acid delaying ethylene production,and decreasing the peak level of respiration rate.it also inhibited the reduction of total soluble solid,titratable acid,and vitamin C,maintains catalase and peroxidase activity.based on the present study,the 4%dehydration treatment was the most effective,which extended the storage period up to 60 days and reduced the decay rate to 33%.in conclusion,it was revealed that STPD could be an effective method for retarding the losses that occurred in the fruit quality of A.arguta during cold storage.
文摘【目的】分析国内外软枣猕猴桃的研究现状,以期为中国软枣猕猴桃的可持续发展提供建议。【方法】以中国知网(CNKI)1979—2024年和WOS(Web of Science)1966—2024年数据库中软枣猕猴桃学术论文为研究对象,运用CiteSpace、VOSviewer进行文献计量学分析。【结果】统计年限期间,关于软枣猕猴桃的文献共发表论文1283篇(中文823篇,英文460篇);中英文发文量分别从2010年和2016年开始快速上升,且中文的文献数量多于英文;中国在发文机构、作者和期刊数量等方面占据优势;国内主要关注资源调查、品种选育、组织培养、栽培技术、果实品质、功能性等研究,国外倾向于研究软枣猕猴桃功能性、基因表达、贮藏性能、品种选育、组织培养等方面。然而,中外在软枣猕猴桃的植物保护等方面的研究均较少,亟须进一步加强。【结论】中国在软枣猕猴桃研究上中、英文发文量占总文献量的75.60%,具有明显优势,研究主要集中在资源与育种、栽培技术、果实品质与功能等领域;国外则偏重于功能性、基因表达、贮藏性能等研究,应加强基础研究与应用研究、产业发展的融合。
基金The Innovative Program for Fruit Tree Research of Liaoning Province, China (LNGSCYTX-13/14-4) supported this study
文摘The fruit of Actinidia arguta is typically climacteric.During ripening,the fruits produce a large amount of ethylene.Although the molecular basis of climacteric fruit ripening has been extensively studied,some aspects such as its transcriptional regulation remain unclear.Here,we compared the transcriptomes of A.arguta fruits collected at commercial harvest(sample d0)and at 6 days after harvest(sample d6)using RNA-seq.A total of 3 659 differentially expressed genes(DEGs)between samples d0 and d6 were detected,of which 2 983 and 1 104 could be functionally annotated with Gene Ontology(GO)and Clusters of Orthologous Groups(COG)pathways.DEGs involved in ethylene biosynthesis and signal transduction were identified,including ACO(c79627),ERF061(c105131),and ERF6(c99193).Transcription factors such as ERF,b HLH,NAC,MADS,and MYB were also differentially expressed between samples d0 and d6.Selected DEGs were subjected to further analysis using quantitative reverse transcription-polymerase chain reaction(q RT-PCR),and the results coincided with those of RNA-seq.Our data revealed additional transcription factors that are involved in the regulation of fruit ripening.These results provide useful information for future research on the transcriptional regulation of fruit ripening.
