目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Ar...目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Arg-5-0022对口腔癌细胞增殖能力的影响;OncotRF网站预测tsRNA-Arg-5-0022下游靶基因及潜在的结合位点;Western Blot检测tsRNA-Arg-5-0022对口腔癌细胞中DNA结合抑制因子4(inhibitor of DNA binding 4,ID4)表达水平的影响;双荧光素酶报告基因实验检测tsRNA-Arg-5-0022与ID4的3'UTR区的结合能力;UALCAN数据库分析ID4在头颈鳞癌中的表达水平,并通过RT-PCR在口腔癌组织中进行验证,分析其与tsRNA-Arg-5-0022表达水平的相关性;CCK8和克隆形成实验检测tsRNA-Arg-5-0022通过靶向抑制ID4对口腔癌细胞增殖能力的影响。结果:tsRFun数据库分析结果显示tsRNA-Arg-5-0022在头颈鳞癌中显著上调(P<0.05);RT-PCR结果显示tsRNA-Arg-5-0022在口腔癌中显著上调(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制tsRNA-Arg-5-0022表达(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制口腔癌细胞的增殖能力;OncotRF网站预测结果显示tsRNA-Arg-5-0022与ID4的3'UTR区存在结合位点;Western Blot检测结果显示tsRNA-Arg-5-0022抑制物能够显著升高口腔癌细胞中ID4表达水平(P<0.05);双荧光素酶报告基因检测结果显示,tsRNA-Arg-5-0022能够与ID4的3'UTR区结合;UALCAN数据库分析结果显示ID4在头颈鳞癌中表达水平下调(P<0.05);RT-PCR结果显示ID4在口腔癌中表达水平下调,且与tsRNA-Arg-5-0022表达水平呈负相关关系;CCK8和克隆形成实验结果显示tsRNA-Arg-5-0022能够通过靶向抑制ID4促进口腔癌细胞增殖。结论:tsRNA-Arg-5-0022在口腔癌中高表达,能够通过直接靶向ID4促进口腔癌细胞恶性增殖,是口腔癌潜在的分子标志物。展开更多
The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacteri...The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacterial control.The influence of some parameters in quantification and performance influencing of pathogen demobilization could be considered in disinfection of wastewater.The comparison of Polyvalent phage(NE1)versus Coliphage(NE4)in suppressing a bacterium Escherichia coli(NDM-1:b-lactam-resistant)with UV irradiation was observed the efficacy in reduction of cells in the disinfection and parameter process.The results with the effect of UV-C irradiation on NDM-1 infected with 1%of NE4 showed a decrease of cells from 8×10^(6)to 2×10^(5)in 60 min with UV-C dose.The NDM1(E.coli)was infected with 1%of NE4(Polyvalent Phage)under magnetic stirring for 1 h,the cells count was 8×10^(6).After 1 h in UV-C e×posure,the cells number reached 3×10^(5).The NDM1 that was e×posed in 1 h of UV-C irradiation and then was infected with 1%of NE4.Cells counting were done 24 h after this procedure.These cells were e×posed in UV-C and showed a reduction in the number of cells from 1×10^(8)to 4×10^(5)after 60 min.The results indicate that bacteriophages can mitigate bacteria species,and combined the conventional water disinfection technologies that can support the microbial safety control strategies.展开更多
文摘目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Arg-5-0022对口腔癌细胞增殖能力的影响;OncotRF网站预测tsRNA-Arg-5-0022下游靶基因及潜在的结合位点;Western Blot检测tsRNA-Arg-5-0022对口腔癌细胞中DNA结合抑制因子4(inhibitor of DNA binding 4,ID4)表达水平的影响;双荧光素酶报告基因实验检测tsRNA-Arg-5-0022与ID4的3'UTR区的结合能力;UALCAN数据库分析ID4在头颈鳞癌中的表达水平,并通过RT-PCR在口腔癌组织中进行验证,分析其与tsRNA-Arg-5-0022表达水平的相关性;CCK8和克隆形成实验检测tsRNA-Arg-5-0022通过靶向抑制ID4对口腔癌细胞增殖能力的影响。结果:tsRFun数据库分析结果显示tsRNA-Arg-5-0022在头颈鳞癌中显著上调(P<0.05);RT-PCR结果显示tsRNA-Arg-5-0022在口腔癌中显著上调(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制tsRNA-Arg-5-0022表达(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制口腔癌细胞的增殖能力;OncotRF网站预测结果显示tsRNA-Arg-5-0022与ID4的3'UTR区存在结合位点;Western Blot检测结果显示tsRNA-Arg-5-0022抑制物能够显著升高口腔癌细胞中ID4表达水平(P<0.05);双荧光素酶报告基因检测结果显示,tsRNA-Arg-5-0022能够与ID4的3'UTR区结合;UALCAN数据库分析结果显示ID4在头颈鳞癌中表达水平下调(P<0.05);RT-PCR结果显示ID4在口腔癌中表达水平下调,且与tsRNA-Arg-5-0022表达水平呈负相关关系;CCK8和克隆形成实验结果显示tsRNA-Arg-5-0022能够通过靶向抑制ID4促进口腔癌细胞增殖。结论:tsRNA-Arg-5-0022在口腔癌中高表达,能够通过直接靶向ID4促进口腔癌细胞恶性增殖,是口腔癌潜在的分子标志物。
基金Fundação de Amparo a Pesquisa do Estado de São Paulo(FAPESP)and the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq),São Paulo,Brazil for PhD scholarship(Process N°.141086/2015-7)financial support(Process No.870243/1997-7).
文摘The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacterial control.The influence of some parameters in quantification and performance influencing of pathogen demobilization could be considered in disinfection of wastewater.The comparison of Polyvalent phage(NE1)versus Coliphage(NE4)in suppressing a bacterium Escherichia coli(NDM-1:b-lactam-resistant)with UV irradiation was observed the efficacy in reduction of cells in the disinfection and parameter process.The results with the effect of UV-C irradiation on NDM-1 infected with 1%of NE4 showed a decrease of cells from 8×10^(6)to 2×10^(5)in 60 min with UV-C dose.The NDM1(E.coli)was infected with 1%of NE4(Polyvalent Phage)under magnetic stirring for 1 h,the cells count was 8×10^(6).After 1 h in UV-C e×posure,the cells number reached 3×10^(5).The NDM1 that was e×posed in 1 h of UV-C irradiation and then was infected with 1%of NE4.Cells counting were done 24 h after this procedure.These cells were e×posed in UV-C and showed a reduction in the number of cells from 1×10^(8)to 4×10^(5)after 60 min.The results indicate that bacteriophages can mitigate bacteria species,and combined the conventional water disinfection technologies that can support the microbial safety control strategies.
