This paper takes the water body of Daliao River-Liaodong Bay as the research object,divides it into three regions:river,estuary,and offshore,and analyzes the changes of antibiotics and antibiotic resistance genes(ARGs...This paper takes the water body of Daliao River-Liaodong Bay as the research object,divides it into three regions:river,estuary,and offshore,and analyzes the changes of antibiotics and antibiotic resistance genes(ARGs)from inland rivers to the sea and the environmental impact factors from this perspective.The results showed that in general,the pollution of antibiotics and ARGs in Daliao River-Liaodong Bay belonged to the medium-low level,and levels of antibiotics and ARGs were nd–106.23 ng/L and nd–1.95×10^(8)copies/L,respectively.The concentrations and types of antibiotics and ARGs decreased from inland to sea regions.Analysis of the distributional characteristics of antibiotics and ARGs from a regionalized perspective revealed significant differences among the three regions in sulfonamide antibiotics,tetracycline antibiotics,and dominant ARGs.Sulfonamide antibiotic levels were significantly higher in the estuarine zone than in the riverine and offshore zones;tetracycline antibiotic levels were significantly higher in the riverine and estuarine zones than in the offshore zone.Aminoglycosides were dominant in the riverine and estuarine zones,and macrolides were dominant in the offshore zone.We characterized the effects of environmental factors on the assignment of antibiotics and ARGs and found that overall temperature contributed the most to variation in antibiotics and ARGs;the contribution of dissolved oxygen was the lowest.The estuarine zone was most affected by these factors,followed by the offshore zone and finally the riverine zone.展开更多
目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Ar...目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Arg-5-0022对口腔癌细胞增殖能力的影响;OncotRF网站预测tsRNA-Arg-5-0022下游靶基因及潜在的结合位点;Western Blot检测tsRNA-Arg-5-0022对口腔癌细胞中DNA结合抑制因子4(inhibitor of DNA binding 4,ID4)表达水平的影响;双荧光素酶报告基因实验检测tsRNA-Arg-5-0022与ID4的3'UTR区的结合能力;UALCAN数据库分析ID4在头颈鳞癌中的表达水平,并通过RT-PCR在口腔癌组织中进行验证,分析其与tsRNA-Arg-5-0022表达水平的相关性;CCK8和克隆形成实验检测tsRNA-Arg-5-0022通过靶向抑制ID4对口腔癌细胞增殖能力的影响。结果:tsRFun数据库分析结果显示tsRNA-Arg-5-0022在头颈鳞癌中显著上调(P<0.05);RT-PCR结果显示tsRNA-Arg-5-0022在口腔癌中显著上调(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制tsRNA-Arg-5-0022表达(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制口腔癌细胞的增殖能力;OncotRF网站预测结果显示tsRNA-Arg-5-0022与ID4的3'UTR区存在结合位点;Western Blot检测结果显示tsRNA-Arg-5-0022抑制物能够显著升高口腔癌细胞中ID4表达水平(P<0.05);双荧光素酶报告基因检测结果显示,tsRNA-Arg-5-0022能够与ID4的3'UTR区结合;UALCAN数据库分析结果显示ID4在头颈鳞癌中表达水平下调(P<0.05);RT-PCR结果显示ID4在口腔癌中表达水平下调,且与tsRNA-Arg-5-0022表达水平呈负相关关系;CCK8和克隆形成实验结果显示tsRNA-Arg-5-0022能够通过靶向抑制ID4促进口腔癌细胞增殖。结论:tsRNA-Arg-5-0022在口腔癌中高表达,能够通过直接靶向ID4促进口腔癌细胞恶性增殖,是口腔癌潜在的分子标志物。展开更多
基金supported by the Key R&D Projects in Hainan Province(No.ZDYF2024SHFZ085)Hainan Provincial Joint Project of Sanya Yazhou Bay Science and the Technology City(No.2021CXLH0009)the National Natural Science Foundation of China(No.42376234).
文摘This paper takes the water body of Daliao River-Liaodong Bay as the research object,divides it into three regions:river,estuary,and offshore,and analyzes the changes of antibiotics and antibiotic resistance genes(ARGs)from inland rivers to the sea and the environmental impact factors from this perspective.The results showed that in general,the pollution of antibiotics and ARGs in Daliao River-Liaodong Bay belonged to the medium-low level,and levels of antibiotics and ARGs were nd–106.23 ng/L and nd–1.95×10^(8)copies/L,respectively.The concentrations and types of antibiotics and ARGs decreased from inland to sea regions.Analysis of the distributional characteristics of antibiotics and ARGs from a regionalized perspective revealed significant differences among the three regions in sulfonamide antibiotics,tetracycline antibiotics,and dominant ARGs.Sulfonamide antibiotic levels were significantly higher in the estuarine zone than in the riverine and offshore zones;tetracycline antibiotic levels were significantly higher in the riverine and estuarine zones than in the offshore zone.Aminoglycosides were dominant in the riverine and estuarine zones,and macrolides were dominant in the offshore zone.We characterized the effects of environmental factors on the assignment of antibiotics and ARGs and found that overall temperature contributed the most to variation in antibiotics and ARGs;the contribution of dissolved oxygen was the lowest.The estuarine zone was most affected by these factors,followed by the offshore zone and finally the riverine zone.
文摘目的:分析tsRNA-Arg-5-0022在口腔癌中的异常表达,对口腔癌细胞恶性增殖的影响及其潜在的分子机制。方法:通过tsRFun数据库分析头颈部鳞状细胞癌中差异表达的tsRNAs,并通过RT-PCR在口腔癌组织中进行验证;CCK8和克隆形成实验检测tsRNA-Arg-5-0022对口腔癌细胞增殖能力的影响;OncotRF网站预测tsRNA-Arg-5-0022下游靶基因及潜在的结合位点;Western Blot检测tsRNA-Arg-5-0022对口腔癌细胞中DNA结合抑制因子4(inhibitor of DNA binding 4,ID4)表达水平的影响;双荧光素酶报告基因实验检测tsRNA-Arg-5-0022与ID4的3'UTR区的结合能力;UALCAN数据库分析ID4在头颈鳞癌中的表达水平,并通过RT-PCR在口腔癌组织中进行验证,分析其与tsRNA-Arg-5-0022表达水平的相关性;CCK8和克隆形成实验检测tsRNA-Arg-5-0022通过靶向抑制ID4对口腔癌细胞增殖能力的影响。结果:tsRFun数据库分析结果显示tsRNA-Arg-5-0022在头颈鳞癌中显著上调(P<0.05);RT-PCR结果显示tsRNA-Arg-5-0022在口腔癌中显著上调(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制tsRNA-Arg-5-0022表达(P<0.05);tsRNA-Arg-5-0022抑制物能够显著抑制口腔癌细胞的增殖能力;OncotRF网站预测结果显示tsRNA-Arg-5-0022与ID4的3'UTR区存在结合位点;Western Blot检测结果显示tsRNA-Arg-5-0022抑制物能够显著升高口腔癌细胞中ID4表达水平(P<0.05);双荧光素酶报告基因检测结果显示,tsRNA-Arg-5-0022能够与ID4的3'UTR区结合;UALCAN数据库分析结果显示ID4在头颈鳞癌中表达水平下调(P<0.05);RT-PCR结果显示ID4在口腔癌中表达水平下调,且与tsRNA-Arg-5-0022表达水平呈负相关关系;CCK8和克隆形成实验结果显示tsRNA-Arg-5-0022能够通过靶向抑制ID4促进口腔癌细胞增殖。结论:tsRNA-Arg-5-0022在口腔癌中高表达,能够通过直接靶向ID4促进口腔癌细胞恶性增殖,是口腔癌潜在的分子标志物。
