The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have be...The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have been observed in N.yezoensis,but the mechanism behind them is unclear.In this study,the involvement of reactive oxygen species(ROS)in the process of wounding-induced archeospores formation and release was investigated.Based on ROS fluorescence observations,the blade fragments began to accumulate ROS after 12 h wounding,while no ROS signals were observed in normal blades.Next,when the blade fragments were treated with ROS inhibitor diphenyleneiodonium(DPI),it showed that the production of archeopores was significantly suppressed.Under normal culturing after wounding,78.3%of the fragments could release archeospores,and the ROS fluorescence was enriched in the released archeospores.Under 0.05μM DPI treatment,the percentage of fragments releasing archeospores was dropped to 16.2%,with decreased ROS fluorescence levels.Under 0.1μM DPI treatment,no archeospores were released from the fragments,and ROS fluorescence was also undetectable in the fragments.Our findings proved that ROS are essential for wounding-induced archeospores production,which might play regulatory roles in the cell dedifferentiation of N.yezoensis.展开更多
The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and rele...The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and release of archeospores remained unclear.In this study,two strains of P.yezoensis with similar genetic backgrounds and opposite abilities for archeospores formation were used for genomic and transcriptomic analysis.Based on whole-genome resequencing,a total of 54,439 SNPs and 12,922 InDels were detected.Specifically,211 SNPs and 8 InDels in coding regions could introduce codon change or frameshift mutation,resulted in sequence variations of corresponding encoded proteins.Furthermore,a total of 2888 differentially expressed genes(DEGs)between two strains were identified based on transcriptomic analysis,and 68 DEGs shared SNPs or InDels according to resequencing analysis,which may be associated with the processes related to the formation of archeospores.This study integrates genomic approaches to identify candidate genes and loci related to archeospores formation in P.yezoensis,laying a foundation for elucidating the regulatory mechanisms of archeospores formation and release.展开更多
Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cul- tivate...Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cul- tivated Porphyra yezoensis and P. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown that the single cells of Porphyra yezoensis had typical features of female gamete (carpogonia) but archeospore. Therefore, the female gametes are responsible in developing leafy thalli.展开更多
As an important seedling source,monospores closely associate with yields in nori farming.However,the molecular mechanism underlying differences in monospore production for different strains remains unknown.Comparative...As an important seedling source,monospores closely associate with yields in nori farming.However,the molecular mechanism underlying differences in monospore production for different strains remains unknown.Comparative transcriptome analysis was performed to examine gene expression differences between the spore abundant wild-type strain(WT)and spore deficient mutant(Y1)of Pyropia chauhanii.The WT strain that produces monospores in abundance exhibited more differentially expressed genes(DEGs)in both number and higher fold-changes than the Y1 strain incapable of producing monospores,indicating that the specific regulation of genes is involved in monospore production.Three lists of DEGs were obtained between the two strains using intersection and displayed in Venn diagram:one expressed only in WT strain,another expressed only in Y1 strain,and the third shared in both strains.DEGs annotated as homologous genes of Arabidopsis thaliana in these 3 lists were curated for online functional enrichment analysis on Metascape website.Gene regulatory networks of WT were functionally enriched in the processing,proteolysis,and transport of proteins,especially within the small GTPase protein family,which might be account for the monospore production ability,whereas Y1 were functionally enriched in the metabolism of essential substance and utilization of indispensable energy,which might be account for the rapid growth of blades.We found the differentially enriched gene regulatory networks between strains might be the intrinsic mechanisms of the different monospore production traits.These findings provide novel insights into the genes and regulatory networks associated with monospore production abilities,which are essential for developing accurate breeding technologies for optimal release of monospores and increase of total nori production.展开更多
基金the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2022fjscq01)the National Key Research and Development Program of China for financial support(2018YFD0900606).
文摘The archeospores released from the blades of Neopyropia yezoensis via dedifferentiated vegetative cells have drawn attention both in cultivation and research.Wounding-induced archeospores formation and release have been observed in N.yezoensis,but the mechanism behind them is unclear.In this study,the involvement of reactive oxygen species(ROS)in the process of wounding-induced archeospores formation and release was investigated.Based on ROS fluorescence observations,the blade fragments began to accumulate ROS after 12 h wounding,while no ROS signals were observed in normal blades.Next,when the blade fragments were treated with ROS inhibitor diphenyleneiodonium(DPI),it showed that the production of archeopores was significantly suppressed.Under normal culturing after wounding,78.3%of the fragments could release archeospores,and the ROS fluorescence was enriched in the released archeospores.Under 0.05μM DPI treatment,the percentage of fragments releasing archeospores was dropped to 16.2%,with decreased ROS fluorescence levels.Under 0.1μM DPI treatment,no archeospores were released from the fragments,and ROS fluorescence was also undetectable in the fragments.Our findings proved that ROS are essential for wounding-induced archeospores production,which might play regulatory roles in the cell dedifferentiation of N.yezoensis.
基金supported by the National Key R&D Program of China(2023YFD2400102,2023YFD2400105)the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2022fjscq01).
文摘The archeospores produced from the blades of Pyropia yezoensis could develop into new blades,which is of significant both in cultivation and research.Nevertheless,the molecular mechanisms behind the formation and release of archeospores remained unclear.In this study,two strains of P.yezoensis with similar genetic backgrounds and opposite abilities for archeospores formation were used for genomic and transcriptomic analysis.Based on whole-genome resequencing,a total of 54,439 SNPs and 12,922 InDels were detected.Specifically,211 SNPs and 8 InDels in coding regions could introduce codon change or frameshift mutation,resulted in sequence variations of corresponding encoded proteins.Furthermore,a total of 2888 differentially expressed genes(DEGs)between two strains were identified based on transcriptomic analysis,and 68 DEGs shared SNPs or InDels according to resequencing analysis,which may be associated with the processes related to the formation of archeospores.This study integrates genomic approaches to identify candidate genes and loci related to archeospores formation in P.yezoensis,laying a foundation for elucidating the regulatory mechanisms of archeospores formation and release.
基金This project was sponsored by the NSFC (No. C-0205-05-39770593)
文摘Several papers have reported that part or whole leafy thallus seemingly consisting of zygotospores can give rise to both blades and conchocelis in the same culture of Porphyra. Study on samples of wild and cul- tivated Porphyra yezoensis and P. oligospermatangia were conducted to clarify the origination of the young blades in the culture. It is confirmed that single cells on the blade of both species, which normally intermixed with zygotospores, germinated into young blades. TEM and SEM observation has shown that the single cells of Porphyra yezoensis had typical features of female gamete (carpogonia) but archeospore. Therefore, the female gametes are responsible in developing leafy thalli.
基金supported by the National Key Research and Development Program of China(2018YFD0900606)National Natural Science Foundation of China(31072208)+2 种基金Major Science and Technology Specific Program of Zhejiang Province,China(2016C02055-6)Science and Technology Planning Project of Jiangsu Province,China(BE2018335)Open Program of Key Laboratory of Cultivation and High-value of Marine Organisms in Fujian Province,China(2017fjscq02).
文摘As an important seedling source,monospores closely associate with yields in nori farming.However,the molecular mechanism underlying differences in monospore production for different strains remains unknown.Comparative transcriptome analysis was performed to examine gene expression differences between the spore abundant wild-type strain(WT)and spore deficient mutant(Y1)of Pyropia chauhanii.The WT strain that produces monospores in abundance exhibited more differentially expressed genes(DEGs)in both number and higher fold-changes than the Y1 strain incapable of producing monospores,indicating that the specific regulation of genes is involved in monospore production.Three lists of DEGs were obtained between the two strains using intersection and displayed in Venn diagram:one expressed only in WT strain,another expressed only in Y1 strain,and the third shared in both strains.DEGs annotated as homologous genes of Arabidopsis thaliana in these 3 lists were curated for online functional enrichment analysis on Metascape website.Gene regulatory networks of WT were functionally enriched in the processing,proteolysis,and transport of proteins,especially within the small GTPase protein family,which might be account for the monospore production ability,whereas Y1 were functionally enriched in the metabolism of essential substance and utilization of indispensable energy,which might be account for the rapid growth of blades.We found the differentially enriched gene regulatory networks between strains might be the intrinsic mechanisms of the different monospore production traits.These findings provide novel insights into the genes and regulatory networks associated with monospore production abilities,which are essential for developing accurate breeding technologies for optimal release of monospores and increase of total nori production.
