[Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Method]25 accessions of kernelled apricot and three accessions of edible apricot were s...[Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Method]25 accessions of kernelled apricot and three accessions of edible apricot were selected as experimental materials to screen the repeatable SSR loci with high polymorphism by the use of SSR markers combined with non-denatured polyacrylamide gel electrophoresis.And the effect of different factors on electrophoresis conditions was compared to explore the optimal SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Result]The optimal non-denatured polyacrylamide gel electrophoresis conditions for SSR-PCR were established as follows：polyacrylamide gel concentration 6%,the ratio of acrylamide to bisacrylamide 29∶1,electrophoresis at 1 000 V for 2-3 h,and staining for 15 min within 0.1% AgNO3.[Conclusion]The optimum electrophoresis system has provided some technical foundations to further study the phylogenetic relationship of kernelled apricots by SSR markers.展开更多
With a kernel apricot cultivar Youzhu 07-3 as the test material, the effects of exogenous calcium and calcium inhibitor on the cold resistance of pistils were investigated. In the bud germination and enlargement perio...With a kernel apricot cultivar Youzhu 07-3 as the test material, the effects of exogenous calcium and calcium inhibitor on the cold resistance of pistils were investigated. In the bud germination and enlargement period, exogenous calcium and calcium ion inhibitor were sprayed to the kernel apdcot trees, respectively. At the full-bloom stage, a certain number of flower branches of kernel apricot were sam- pied and subjected to low-temperature stress indoor, and the soluble sugar content, MDA content, SOD activity and POD activity in the pistils were determined. The results showed that exogenous calcium increased the soluble sugar content, reduced the MDA content and improved the SOD and POD activity in pistils, and it significantly relieved the cold damage to pistils and improved the cold resistance of pistils; while exogenous calcium ion inhibitor showed the opposite effects.展开更多
目的:观察苇茎汤合麻杏石甘汤加减对卒中相关性肺炎(stroke-associated pneumonia,SAP)患者肺部感染评分、炎症因子水平的影响。方法:选取2022年12月至2024年9月郑州市中心医院收治的SAP患者60例,按照随机数字表法分为西医组和中西联合...目的:观察苇茎汤合麻杏石甘汤加减对卒中相关性肺炎(stroke-associated pneumonia,SAP)患者肺部感染评分、炎症因子水平的影响。方法:选取2022年12月至2024年9月郑州市中心医院收治的SAP患者60例,按照随机数字表法分为西医组和中西联合组,每组各30例。西医组给予常规西医对症治疗,中西联合组在西医组治疗的基础上加用苇茎汤合麻杏石甘汤加减治疗。观察两组患者治疗前后临床肺部感染评分(clinical pulmonary infection score,CPIS)、Barthel指数、美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分、炎症因子[白细胞计数(white blood cell,WBC)、降钙素原(procalcitonin,PCT)、白细胞介素(interleukin,IL)-17、高迁移率族蛋白B1(high mobility group box 1 protein,HMGB1)]及临床疗效、临床指标、不良反应。结果:中西联合组有效率为93.33%,西医组有效率为73.33%,中西联合组有效率高于西医组,差异有统计学意义(P<0.05);两组治疗后中医症状积分均低于治疗前,且中西联合组治疗后中医症状积分低于西医组,差异有统计学意义(P<0.05);中西联合组退热、咳痰消失、肺部啰音消失及抗生素使用时间短于西医组,差异有统计学意义(P<0.05);两组治疗后CPIS评分均低于治疗前,且中西联合组治疗后CPIS评分低于西医组,差异有统计学意义(P<0.05);两组治疗后WBC、PCT、IL-17、HMGB1均低于治疗前,且中西联合组治疗后低于西医组,差异有统计学意义(P<0.05);两组治疗后Barthel指数均高于治疗前,NIHSS评分均低于治疗前,差异有统计学意义(P<0.05),且中西联合组治疗后Barthel指数高于西医组,NIHSS评分低于西医组,差异有统计学意义(P<0.05);治疗期间,西医组患者有1例出现头晕,中西联合组患者有2例出现腹胀,均未出现严重不良反应,组间比较差异无统计学意义(P>0.05)。结论:苇茎汤合麻杏石甘汤加减可有效改善SAP患者临床症状,显著降低肺部感染评分和炎症因子水平。展开更多
基金Supported by the Scientific and Technological Program of Educational Department of Hebei Province(No.ZH2007116)~~
文摘[Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Method]25 accessions of kernelled apricot and three accessions of edible apricot were selected as experimental materials to screen the repeatable SSR loci with high polymorphism by the use of SSR markers combined with non-denatured polyacrylamide gel electrophoresis.And the effect of different factors on electrophoresis conditions was compared to explore the optimal SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot.[Result]The optimal non-denatured polyacrylamide gel electrophoresis conditions for SSR-PCR were established as follows：polyacrylamide gel concentration 6%,the ratio of acrylamide to bisacrylamide 29∶1,electrophoresis at 1 000 V for 2-3 h,and staining for 15 min within 0.1% AgNO3.[Conclusion]The optimum electrophoresis system has provided some technical foundations to further study the phylogenetic relationship of kernelled apricots by SSR markers.
基金Supported by Youth Foundation of Hebei Academy of Agriculture and Forestry Sciences(A2012100107)~~
文摘With a kernel apricot cultivar Youzhu 07-3 as the test material, the effects of exogenous calcium and calcium inhibitor on the cold resistance of pistils were investigated. In the bud germination and enlargement period, exogenous calcium and calcium ion inhibitor were sprayed to the kernel apdcot trees, respectively. At the full-bloom stage, a certain number of flower branches of kernel apricot were sam- pied and subjected to low-temperature stress indoor, and the soluble sugar content, MDA content, SOD activity and POD activity in the pistils were determined. The results showed that exogenous calcium increased the soluble sugar content, reduced the MDA content and improved the SOD and POD activity in pistils, and it significantly relieved the cold damage to pistils and improved the cold resistance of pistils; while exogenous calcium ion inhibitor showed the opposite effects.
文摘目的:观察苇茎汤合麻杏石甘汤加减对卒中相关性肺炎(stroke-associated pneumonia,SAP)患者肺部感染评分、炎症因子水平的影响。方法:选取2022年12月至2024年9月郑州市中心医院收治的SAP患者60例,按照随机数字表法分为西医组和中西联合组,每组各30例。西医组给予常规西医对症治疗,中西联合组在西医组治疗的基础上加用苇茎汤合麻杏石甘汤加减治疗。观察两组患者治疗前后临床肺部感染评分(clinical pulmonary infection score,CPIS)、Barthel指数、美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分、炎症因子[白细胞计数(white blood cell,WBC)、降钙素原(procalcitonin,PCT)、白细胞介素(interleukin,IL)-17、高迁移率族蛋白B1(high mobility group box 1 protein,HMGB1)]及临床疗效、临床指标、不良反应。结果:中西联合组有效率为93.33%,西医组有效率为73.33%,中西联合组有效率高于西医组,差异有统计学意义(P<0.05);两组治疗后中医症状积分均低于治疗前,且中西联合组治疗后中医症状积分低于西医组,差异有统计学意义(P<0.05);中西联合组退热、咳痰消失、肺部啰音消失及抗生素使用时间短于西医组,差异有统计学意义(P<0.05);两组治疗后CPIS评分均低于治疗前,且中西联合组治疗后CPIS评分低于西医组,差异有统计学意义(P<0.05);两组治疗后WBC、PCT、IL-17、HMGB1均低于治疗前,且中西联合组治疗后低于西医组,差异有统计学意义(P<0.05);两组治疗后Barthel指数均高于治疗前,NIHSS评分均低于治疗前,差异有统计学意义(P<0.05),且中西联合组治疗后Barthel指数高于西医组,NIHSS评分低于西医组,差异有统计学意义(P<0.05);治疗期间,西医组患者有1例出现头晕,中西联合组患者有2例出现腹胀,均未出现严重不良反应,组间比较差异无统计学意义(P>0.05)。结论:苇茎汤合麻杏石甘汤加减可有效改善SAP患者临床症状,显著降低肺部感染评分和炎症因子水平。
