Fullerene(C_(7)0),a promising new photosensitizer,faces challenges in its biological applications due to its extreme hydrophobicity.In order to enhance the solubility of fullerene(C_(7)0)and facilitate its biological ...Fullerene(C_(7)0),a promising new photosensitizer,faces challenges in its biological applications due to its extreme hydrophobicity.In order to enhance the solubility of fullerene(C_(7)0)and facilitate its biological applications,we synthesized a novel hydroxyl-modified fullerene compound(C_(7)0(OH)_(8))with excellent photosensitizing properties.The introduction of hydroxyl groups allows it to self-assemble with DSPE-PEG(2000);therefore,we prepared it as a nanomedicine(C_(7)0(OH)_(8)@NP).Under white light irradiation,C_(7)0(OH)_(8)@NP stimulates the production of reactive oxygen species(ROS).Furthermore,results have demonstrated that a substantial amount of ROS can also be generated within cells,resulting in cell death.We found that C_(7)0(OH)_(8)@NP can induce both apoptosis and pyroptosis in HeLa cells and identified its mechanism of cell death through the activation of caspase 3/gasdermin E pathways.Importantly,C_(7)0(OH)_(8)@NP demonstrates significant anti-tumor activity in a nude mouse tumor-bearing model.These results highlight the potential of novel fullerene compounds as photodynamic therapy agents.展开更多
Tetrabutylammonium ions(TBA+)have commonly been used to exfoliate RuO_(2)into monolayers via ion exchange reactions.However,the low production yield of RuO_(2)exfoliation,which originates from the large molecular size...Tetrabutylammonium ions(TBA+)have commonly been used to exfoliate RuO_(2)into monolayers via ion exchange reactions.However,the low production yield of RuO_(2)exfoliation,which originates from the large molecular size of TBA^(+),limits wider utilisation of RuO_(2)monolayers in optoelectronic applications.We introduce a rapid and efficient dual-step exfoliation process beginning with intercalation of small organic molecules(tetramethylammonium ions)into RuO_(2),which is followed by the addition of TBA^(+)as a second intercalant to realize RuO_(2)monolayer production.Our dual-step intercalation process increases the RuO_(2)monolayer exfoliation yield from 9.9%to 60%after 14 days.Density functional theory calculations reveal that the activation energy of dual-step intercalation is much lower than that of direct intercalation of TBA^(+)ions into the RuO_(2)structure.The experimental and theoretical results of dual-step intercalation suggest that it is a facile and general approach for the production of metal oxide monolayers,and could widen the use of metal oxide monolayer nanosheets.展开更多
基金support from the National Natural Science Foundation of China(22131003)support from the Beijing National Laboratory for Molecular Sciences(BNLMS-CXXM-201904)+1 种基金the National Natural Science Foundation of China(22362029)support from the Science Research Foundation of Jilin Province(YDZJ202301ZYTS478).
文摘Fullerene(C_(7)0),a promising new photosensitizer,faces challenges in its biological applications due to its extreme hydrophobicity.In order to enhance the solubility of fullerene(C_(7)0)and facilitate its biological applications,we synthesized a novel hydroxyl-modified fullerene compound(C_(7)0(OH)_(8))with excellent photosensitizing properties.The introduction of hydroxyl groups allows it to self-assemble with DSPE-PEG(2000);therefore,we prepared it as a nanomedicine(C_(7)0(OH)_(8)@NP).Under white light irradiation,C_(7)0(OH)_(8)@NP stimulates the production of reactive oxygen species(ROS).Furthermore,results have demonstrated that a substantial amount of ROS can also be generated within cells,resulting in cell death.We found that C_(7)0(OH)_(8)@NP can induce both apoptosis and pyroptosis in HeLa cells and identified its mechanism of cell death through the activation of caspase 3/gasdermin E pathways.Importantly,C_(7)0(OH)_(8)@NP demonstrates significant anti-tumor activity in a nude mouse tumor-bearing model.These results highlight the potential of novel fullerene compounds as photodynamic therapy agents.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2019R1A6A1A11055660,NRF-2019R1C1C1005632,and NRF-2017R1D1A1B03034322).
文摘Tetrabutylammonium ions(TBA+)have commonly been used to exfoliate RuO_(2)into monolayers via ion exchange reactions.However,the low production yield of RuO_(2)exfoliation,which originates from the large molecular size of TBA^(+),limits wider utilisation of RuO_(2)monolayers in optoelectronic applications.We introduce a rapid and efficient dual-step exfoliation process beginning with intercalation of small organic molecules(tetramethylammonium ions)into RuO_(2),which is followed by the addition of TBA^(+)as a second intercalant to realize RuO_(2)monolayer production.Our dual-step intercalation process increases the RuO_(2)monolayer exfoliation yield from 9.9%to 60%after 14 days.Density functional theory calculations reveal that the activation energy of dual-step intercalation is much lower than that of direct intercalation of TBA^(+)ions into the RuO_(2)structure.The experimental and theoretical results of dual-step intercalation suggest that it is a facile and general approach for the production of metal oxide monolayers,and could widen the use of metal oxide monolayer nanosheets.
