[Objective] cDNA from squalene synthase was cloned and sequenced.[Method] A pair of specific primers was designed according to the cDNA gene sequence of squalene synthase published in GenBank.Total RNA was extracted f...[Objective] cDNA from squalene synthase was cloned and sequenced.[Method] A pair of specific primers was designed according to the cDNA gene sequence of squalene synthase published in GenBank.Total RNA was extracted from the cell of Artemisia apiacea.The genes of squalene synthase were amplified by using RT-PCR.It was connected with pMD19-T vector and the cloned fragment sequences were analyzed.[Result] SS gene with the whole length of 1 257 bp was amplified and the fragment encoded 418 amino acids.The homo...展开更多
Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops.Heat shock transcription factors(Hsf)play important roles in heat tolerance during plant development.Here,we conducted ...Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops.Heat shock transcription factors(Hsf)play important roles in heat tolerance during plant development.Here,we conducted systematic analyses of the Hsf gene family in three Apiaceae species,including 17 Apium graveolens(celery),32 Coriandrum sativum(coriander),and 14 Daucus carota(carrot).A total of 73 Hsf genes were identified in three representative species,including Arabidopsis thaliana,Vitis vinifera,and Lactuca sativa.Whole-genome duplication played important roles in the Hsf gene family’s expansion within Apiaceae.Interestingly,we found that coriander had more Hsf genes than celery and carrot due to greater expansion and fewer losses.Twenty-seven branches of the phylogenetic tree underwent considerable positive selection in these Apiaceae species.We also explored the expression patterns of Hsf genes in three plant organs.Collectively,this study will serve as a rich gene resource for exploring the molecular mechanisms of heat tolerance.Additionally,this is the first study to report on the Hsf gene family in Apiaceae;thus,our research will provide guidance for future comparative and functional genomic studies on the Hsf gene family and others in Apiaceae.展开更多
To investigate the taxonomic value of leaf epidermal characters in developing a classification for Ligusticum (Apiaceae), thirty-eight species (including a cultivar) of the genus were examined using both light and sca...To investigate the taxonomic value of leaf epidermal characters in developing a classification for Ligusticum (Apiaceae), thirty-eight species (including a cultivar) of the genus were examined using both light and scanning electron microscopy. Our results show that the infrageneric classification of Ligusticum based on the characters of involucel bracteoles, mericarps, and pollen grains was not supported as few epidermal characters were relatively constant at section level. Evidence from leaf epidermis, similar to that from molecular and morphological data, supports the separation of Tilingia (i.e. L. ajanense and L. tachiroei) from Ligusticum. Ligusticopsis was found to be indistinguishable from Ligusticum in epidermal characters. Furthermore, results from epidermal analysis, together with that from cytological evidence, fruit morphology as well as molecular phylogenetics suggest that L. delavayi should be transferred out of Ligusticum.展开更多
Our study aimed to compare the essential oil(EO)concentration and composition of several Apiaceae species growing in the Northern region of Vietnam.The yields of EOs from materials ranged from 0.03%(root EO of Angelic...Our study aimed to compare the essential oil(EO)concentration and composition of several Apiaceae species growing in the Northern region of Vietnam.The yields of EOs from materials ranged from 0.03%(root EO of Angelica acutiloba and aerial parts EO of Heracleum bivittatum)-0.27%(leaf EO of Xyloselinum vietnamense).Gas chromatography-mass spectrometry(GC-MS)allowed the identification of 74 components in the EOs of six Apiaceae species,making up 94.4%–100.0%of the oils.In EO from Angelica acutiloba,(Z)-ligustilide accounted for an extremely large proportion(94.9%).EO of Angelica pubescens was dominated by six character-istic components includingα-pinene(21.5%),β-phellandrene(18.1%),p-cymene(12.2%),3-methylnonane(8.7%),o-cymene(8.1%),and D-sylvestrene(6.2%).The EO from Cryptotaenia japonica was characterized by high amounts ofα-selinene(48.7%),β-selinene(23.7%),and trans-β-farnesene(5.4%).The EOs from leaves and stems of Xyloselinum vietnamense were characterized by high concentrations of sabinene(69.8%and 33.8%),4-terpineol(8.7%and 7.4%)andβ-pinene(4.0%and 6.5%)while EOs from aerial parts and root of Xylo-selinum leonidii comprise four characteristic monoterpenes includingα-pinene(28.2%and 52.8%),β-pinene(7.9%and 10.3%),β-phellandrene(7.6%and 15.3%),and sabinene(3.0%and 4.1%).Additionally,cryptone is also one of the major components in the EO of Xyloselinum leonidii(13.2%in the aerial parts oil and 2.8%in the root oil).In the EOs isolated from the aerial parts and root of Heracleum bivittatum,α-pinene(22.5%and 70.2%)andβ-pinene(43.2%and 20.0%)were the predominant monoterpenes.Sabinene appeared in the EO from aerial parts of Heracleum bivittatum with a relatively high concentration(13.5%)while bornyl acetate(5.1%)was also one of the main components in the EO from its aerial parts but was not detected in other Apiaceae species in the present study.These databases help identify and control the quality of plant material studied from the family Apiaceae growing in Vietnam.展开更多
The article presents materials on the distribution and data on the chemical composition of monocarpic plants of the Apiaceae family in the Tien Shan Mountains.
Aedes aegypti mosquitoes are responsible for transmission of many viral diseases, such as Zika fever, dengue fever, yellow fever, and chikungunya. Emergence of resistance to currently used pesticides among mosquitoes ...Aedes aegypti mosquitoes are responsible for transmission of many viral diseases, such as Zika fever, dengue fever, yellow fever, and chikungunya. Emergence of resistance to currently used pesticides among mosquitoes has increased the importance for the search for novel mosquito control agents. Natural products, particularly plant and microbe derived secondary metabolites, are good sources in the search for such compounds. Ammi visnaga (Lam.) Lamarck is a plant in the Apiaceae family native to North Africa, Europe, and Asia. In the search for environmentally benign and effective insecticides as part of an ongoing joint effort between the USDA (US Department of Agriculture) and the DWFP (Deployed War Fighter Protection) program sponsored by the Department of Defense, we have investigated ethyl acetate extract of A. visnaga seeds. Two furanochromones, khellin and visnagin that exhibited larvicide activity against Aedes aegypti mosquito larvae were isolated from the ethyl acetate extract of the seeds by bioassay-guided fractionation. This is the first report of mosquito larvicidal activity of khellin and visnagin.展开更多
Despite their remarkable content of biologically active compounds,highly valuable for human health,wild relatives of Umbelliferous plants show limited utilization.The aim of the present work was the evaluation of the ...Despite their remarkable content of biologically active compounds,highly valuable for human health,wild relatives of Umbelliferous plants show limited utilization.The aim of the present work was the evaluation of the antioxidant status of Anthriscus,Chaerophyllum,and Myrrhoides species gathered in different climatic zones(from Mediterranean to Arctic)and of their suitability to produce valuable functional food for optimizing the human Se status.Among the Crimean plants,A.sylvestris,C.bulbosis,and M.nososa showed the highest antioxidant status,while the lowest was recorded in A.cerefolium and A.caucalis,displaying a significant correlation between the antioxidant activity(AOA)and polyphenols(TP)(r=0.93;p<0.001).A positive correlation between the longitude and AOA,and TP was detected for A.sylvestris(r=0.95 and r=0.93,respectively;p<0.001).The high adaptability and wide geographical distribution of the latter species,as well as its significant content in natural antioxidants,make it an interesting product for Se biofortification.Foliar supplementation of sodium selenate allowed to obtain a new functional food with high TP content(36.4 mg GAE g^(−1) d.w.),ascorbic acid(42 mg 100 g^(−1) f.w.),and AOA(72 mg GAE g^(−1) d.w.).Moreover,Se level exceeded 3 mg kg^(−1) d.w.,which suggests the plant suitability for the human Se status optimization,especially in Se-deficient Arctic zone,particularly referring to Nikel settlement with relatively low levels of Se in human hair(377±13μg kg^(−1)),bread(58±3μg kg^(−1)),and freshwater fish(359±22μg kg^(−1)).The high antioxidant status of Myrrhoides nodosa indicates the need for detailed investigation of plant biochemistry and the identification of its utilization prospects.展开更多
文摘[Objective] cDNA from squalene synthase was cloned and sequenced.[Method] A pair of specific primers was designed according to the cDNA gene sequence of squalene synthase published in GenBank.Total RNA was extracted from the cell of Artemisia apiacea.The genes of squalene synthase were amplified by using RT-PCR.It was connected with pMD19-T vector and the cloned fragment sequences were analyzed.[Result] SS gene with the whole length of 1 257 bp was amplified and the fragment encoded 418 amino acids.The homo...
基金supported by the National Natural Science Foundation of China(Grant No.31801856)Hebei Province Higher Education Youth Talents Program(Grant No.BJ2018016)+1 种基金the China Postdoctoral Science Foundation(Grant No.2020M673188)the innovation and entrepreneurship training program for college students of North China University of Science and Technology(Grant No.X2019256).
文摘Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops.Heat shock transcription factors(Hsf)play important roles in heat tolerance during plant development.Here,we conducted systematic analyses of the Hsf gene family in three Apiaceae species,including 17 Apium graveolens(celery),32 Coriandrum sativum(coriander),and 14 Daucus carota(carrot).A total of 73 Hsf genes were identified in three representative species,including Arabidopsis thaliana,Vitis vinifera,and Lactuca sativa.Whole-genome duplication played important roles in the Hsf gene family’s expansion within Apiaceae.Interestingly,we found that coriander had more Hsf genes than celery and carrot due to greater expansion and fewer losses.Twenty-seven branches of the phylogenetic tree underwent considerable positive selection in these Apiaceae species.We also explored the expression patterns of Hsf genes in three plant organs.Collectively,this study will serve as a rich gene resource for exploring the molecular mechanisms of heat tolerance.Additionally,this is the first study to report on the Hsf gene family in Apiaceae;thus,our research will provide guidance for future comparative and functional genomic studies on the Hsf gene family and others in Apiaceae.
文摘To investigate the taxonomic value of leaf epidermal characters in developing a classification for Ligusticum (Apiaceae), thirty-eight species (including a cultivar) of the genus were examined using both light and scanning electron microscopy. Our results show that the infrageneric classification of Ligusticum based on the characters of involucel bracteoles, mericarps, and pollen grains was not supported as few epidermal characters were relatively constant at section level. Evidence from leaf epidermis, similar to that from molecular and morphological data, supports the separation of Tilingia (i.e. L. ajanense and L. tachiroei) from Ligusticum. Ligusticopsis was found to be indistinguishable from Ligusticum in epidermal characters. Furthermore, results from epidermal analysis, together with that from cytological evidence, fruit morphology as well as molecular phylogenetics suggest that L. delavayi should be transferred out of Ligusticum.
基金supported by a grant from the Vietnam Academy of Science and Technology,Project Code CSCL09.03/23-24.
文摘Our study aimed to compare the essential oil(EO)concentration and composition of several Apiaceae species growing in the Northern region of Vietnam.The yields of EOs from materials ranged from 0.03%(root EO of Angelica acutiloba and aerial parts EO of Heracleum bivittatum)-0.27%(leaf EO of Xyloselinum vietnamense).Gas chromatography-mass spectrometry(GC-MS)allowed the identification of 74 components in the EOs of six Apiaceae species,making up 94.4%–100.0%of the oils.In EO from Angelica acutiloba,(Z)-ligustilide accounted for an extremely large proportion(94.9%).EO of Angelica pubescens was dominated by six character-istic components includingα-pinene(21.5%),β-phellandrene(18.1%),p-cymene(12.2%),3-methylnonane(8.7%),o-cymene(8.1%),and D-sylvestrene(6.2%).The EO from Cryptotaenia japonica was characterized by high amounts ofα-selinene(48.7%),β-selinene(23.7%),and trans-β-farnesene(5.4%).The EOs from leaves and stems of Xyloselinum vietnamense were characterized by high concentrations of sabinene(69.8%and 33.8%),4-terpineol(8.7%and 7.4%)andβ-pinene(4.0%and 6.5%)while EOs from aerial parts and root of Xylo-selinum leonidii comprise four characteristic monoterpenes includingα-pinene(28.2%and 52.8%),β-pinene(7.9%and 10.3%),β-phellandrene(7.6%and 15.3%),and sabinene(3.0%and 4.1%).Additionally,cryptone is also one of the major components in the EO of Xyloselinum leonidii(13.2%in the aerial parts oil and 2.8%in the root oil).In the EOs isolated from the aerial parts and root of Heracleum bivittatum,α-pinene(22.5%and 70.2%)andβ-pinene(43.2%and 20.0%)were the predominant monoterpenes.Sabinene appeared in the EO from aerial parts of Heracleum bivittatum with a relatively high concentration(13.5%)while bornyl acetate(5.1%)was also one of the main components in the EO from its aerial parts but was not detected in other Apiaceae species in the present study.These databases help identify and control the quality of plant material studied from the family Apiaceae growing in Vietnam.
文摘The article presents materials on the distribution and data on the chemical composition of monocarpic plants of the Apiaceae family in the Tien Shan Mountains.
文摘Aedes aegypti mosquitoes are responsible for transmission of many viral diseases, such as Zika fever, dengue fever, yellow fever, and chikungunya. Emergence of resistance to currently used pesticides among mosquitoes has increased the importance for the search for novel mosquito control agents. Natural products, particularly plant and microbe derived secondary metabolites, are good sources in the search for such compounds. Ammi visnaga (Lam.) Lamarck is a plant in the Apiaceae family native to North Africa, Europe, and Asia. In the search for environmentally benign and effective insecticides as part of an ongoing joint effort between the USDA (US Department of Agriculture) and the DWFP (Deployed War Fighter Protection) program sponsored by the Department of Defense, we have investigated ethyl acetate extract of A. visnaga seeds. Two furanochromones, khellin and visnagin that exhibited larvicide activity against Aedes aegypti mosquito larvae were isolated from the ethyl acetate extract of the seeds by bioassay-guided fractionation. This is the first report of mosquito larvicidal activity of khellin and visnagin.
文摘Despite their remarkable content of biologically active compounds,highly valuable for human health,wild relatives of Umbelliferous plants show limited utilization.The aim of the present work was the evaluation of the antioxidant status of Anthriscus,Chaerophyllum,and Myrrhoides species gathered in different climatic zones(from Mediterranean to Arctic)and of their suitability to produce valuable functional food for optimizing the human Se status.Among the Crimean plants,A.sylvestris,C.bulbosis,and M.nososa showed the highest antioxidant status,while the lowest was recorded in A.cerefolium and A.caucalis,displaying a significant correlation between the antioxidant activity(AOA)and polyphenols(TP)(r=0.93;p<0.001).A positive correlation between the longitude and AOA,and TP was detected for A.sylvestris(r=0.95 and r=0.93,respectively;p<0.001).The high adaptability and wide geographical distribution of the latter species,as well as its significant content in natural antioxidants,make it an interesting product for Se biofortification.Foliar supplementation of sodium selenate allowed to obtain a new functional food with high TP content(36.4 mg GAE g^(−1) d.w.),ascorbic acid(42 mg 100 g^(−1) f.w.),and AOA(72 mg GAE g^(−1) d.w.).Moreover,Se level exceeded 3 mg kg^(−1) d.w.,which suggests the plant suitability for the human Se status optimization,especially in Se-deficient Arctic zone,particularly referring to Nikel settlement with relatively low levels of Se in human hair(377±13μg kg^(−1)),bread(58±3μg kg^(−1)),and freshwater fish(359±22μg kg^(−1)).The high antioxidant status of Myrrhoides nodosa indicates the need for detailed investigation of plant biochemistry and the identification of its utilization prospects.
