Objective:To explore the anti-photoaging properties of salvianolic acid B(Sal B).Methods:The optimal photoaging model of human immortalized keratinocytes(HaCaaT cells)were constructed by expose to ultraviolet B(UVB)ra...Objective:To explore the anti-photoaging properties of salvianolic acid B(Sal B).Methods:The optimal photoaging model of human immortalized keratinocytes(HaCaaT cells)were constructed by expose to ultraviolet B(UVB)radiation.The cells were divided into control,model and different concentrations of Sal B groups.Cell viability was measured via cell counting kit-8.Subsequently,the levels of oxidative stress,including reactive oxygen species(ROS),hydroxyproline(Hyp),catalase(CAT),and glutathione peroxidase(GSH-Px)were detected using the relevant kits.Silent information regulator 1(SIRT1)protein level was detected using Western blot.The binding pattern of Sal B and SIRT1 was determined via molecular docking.Results:Sal B significantly increased the viability of UVB-irradiated HaCaT cells(P<0.05 or P<0.01).Sal B effectively scavenged the accumulation of ROS induced by UVB(P<0.05 or P<0.01).In addition,Sal B modulated oxidative stress by increasing the intracellular concentrations of Hyp and CAT and the activity of GSH-Px(P<0.05 or P<0.01).The Western blot results revealed a substantial increase in SIRT1 protein levels following Sal B administration(P<0.05).Moreover,Sal B exhibited good binding affinity toward SIRT1,with a docking energy of-7.5 kCal/mol.Conclusion:Sal B could improve the repair of photodamaged cells by alleviating cellular oxidative stress and regulating the expression of SIRT1 protein.展开更多
基金Supported by the National Natural Science Foundation of China(No.82173982)the Guangdong Natural Science Project(No.2022A1515011382)。
文摘Objective:To explore the anti-photoaging properties of salvianolic acid B(Sal B).Methods:The optimal photoaging model of human immortalized keratinocytes(HaCaaT cells)were constructed by expose to ultraviolet B(UVB)radiation.The cells were divided into control,model and different concentrations of Sal B groups.Cell viability was measured via cell counting kit-8.Subsequently,the levels of oxidative stress,including reactive oxygen species(ROS),hydroxyproline(Hyp),catalase(CAT),and glutathione peroxidase(GSH-Px)were detected using the relevant kits.Silent information regulator 1(SIRT1)protein level was detected using Western blot.The binding pattern of Sal B and SIRT1 was determined via molecular docking.Results:Sal B significantly increased the viability of UVB-irradiated HaCaT cells(P<0.05 or P<0.01).Sal B effectively scavenged the accumulation of ROS induced by UVB(P<0.05 or P<0.01).In addition,Sal B modulated oxidative stress by increasing the intracellular concentrations of Hyp and CAT and the activity of GSH-Px(P<0.05 or P<0.01).The Western blot results revealed a substantial increase in SIRT1 protein levels following Sal B administration(P<0.05).Moreover,Sal B exhibited good binding affinity toward SIRT1,with a docking energy of-7.5 kCal/mol.Conclusion:Sal B could improve the repair of photodamaged cells by alleviating cellular oxidative stress and regulating the expression of SIRT1 protein.
