The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by establish...The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by established in-vitro agar diffusion method.The methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different polarities.The agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g(+and-)microbial strains.Antimicrobial potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial strains.The highest activity chloroform extract was analysed by column chromatography(CC)for the isolation of antimicrobial compounds.The structure of the isolated compounds was characterized based on 1D,2D NMR and MS spectra.In conclusion,purest compounds might be useful as a remedy for infectious diseases.展开更多
Soybean,a crucial global leguminous crop,confronts persistent threats from diverse pathogens,exerting a profound impact on global yields.While genetic dimensions of soybean-pathogen interactions have garnered attentio...Soybean,a crucial global leguminous crop,confronts persistent threats from diverse pathogens,exerting a profound impact on global yields.While genetic dimensions of soybean-pathogen interactions have garnered attention,the intricate biochemical responses remain poorly elucidated.In this study,we applied targeted and untargeted liquid chromatography coupled to mass spectrometry(LC-MS)metabolite profiling to dissect the complex interplay between soybeans and five distinct pathogens.Our analysis uncovered 627 idMS/MS spectra,leading to the identification of four main modules,encompassing flavonoids,isoflavonoids,triterpenoids,and amino acids and peptides,alongside other compounds such as phenolics.Profound shifts were observed in both primary and secondary metabolism in response to pathogenic infections.Particularly notable were the bidirectional changes in total flavonoids across diverse pathogenic inoculations,while triterpenoids exhibited a general declining trend.Noteworthy among the highly inducible total flavonoids were known representative antipathogen compounds(glyceollin I),backbone forms of isoflavonoids(daidzein,genistein,glycitein,formononetin),and newly purified compounds in this study(prunin).Subsequently,we delved into the biological roles of these five compounds,validating their diverse functions against pathogens:prunin significantly inhibited the vegetative growth and virulence of Phytophthora sojae;genistein exhibited a pronounced inhibitory effect on the vegetative growth and virulence of Phomopsis longicolla;daidzein and formononetin displayed significant repressive effects on the virulence of P.longicolla.This study underscores the potent utility of metabolomic tools,providing in-depth insights into plant-pathogen interactions from a biochemical perspective.The findings not only contribute to plant pathology but also offer strategic pathways for bolstering plant resistance against diseases on a broader scale.展开更多
文摘The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by established in-vitro agar diffusion method.The methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different polarities.The agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g(+and-)microbial strains.Antimicrobial potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial strains.The highest activity chloroform extract was analysed by column chromatography(CC)for the isolation of antimicrobial compounds.The structure of the isolated compounds was characterized based on 1D,2D NMR and MS spectra.In conclusion,purest compounds might be useful as a remedy for infectious diseases.
基金supported by the National Natural Science Foundation of China(32100044)the Jiangsu“Innovative and Entrepreneurial Talent”program(JSSCRC2021510)+1 种基金the Fundamental Research Funds for the Central Universities(KYT2023005)supported by the high-performance computing platform of Bioinformatics Center,Nanjing Agricultural University。
文摘Soybean,a crucial global leguminous crop,confronts persistent threats from diverse pathogens,exerting a profound impact on global yields.While genetic dimensions of soybean-pathogen interactions have garnered attention,the intricate biochemical responses remain poorly elucidated.In this study,we applied targeted and untargeted liquid chromatography coupled to mass spectrometry(LC-MS)metabolite profiling to dissect the complex interplay between soybeans and five distinct pathogens.Our analysis uncovered 627 idMS/MS spectra,leading to the identification of four main modules,encompassing flavonoids,isoflavonoids,triterpenoids,and amino acids and peptides,alongside other compounds such as phenolics.Profound shifts were observed in both primary and secondary metabolism in response to pathogenic infections.Particularly notable were the bidirectional changes in total flavonoids across diverse pathogenic inoculations,while triterpenoids exhibited a general declining trend.Noteworthy among the highly inducible total flavonoids were known representative antipathogen compounds(glyceollin I),backbone forms of isoflavonoids(daidzein,genistein,glycitein,formononetin),and newly purified compounds in this study(prunin).Subsequently,we delved into the biological roles of these five compounds,validating their diverse functions against pathogens:prunin significantly inhibited the vegetative growth and virulence of Phytophthora sojae;genistein exhibited a pronounced inhibitory effect on the vegetative growth and virulence of Phomopsis longicolla;daidzein and formononetin displayed significant repressive effects on the virulence of P.longicolla.This study underscores the potent utility of metabolomic tools,providing in-depth insights into plant-pathogen interactions from a biochemical perspective.The findings not only contribute to plant pathology but also offer strategic pathways for bolstering plant resistance against diseases on a broader scale.
