目的分析副溶血弧菌O10:K4血清型O抗原基因簇的分子特征,并构建基于基因组的分子血清型预测数据库。方法从美国国家生物技术信息中心Assembly数据库获取副溶血弧菌O抗原基因簇序列,使用blastn、fastANI、Sourmash和p i x y工具分析序列...目的分析副溶血弧菌O10:K4血清型O抗原基因簇的分子特征,并构建基于基因组的分子血清型预测数据库。方法从美国国家生物技术信息中心Assembly数据库获取副溶血弧菌O抗原基因簇序列,使用blastn、fastANI、Sourmash和p i x y工具分析序列相似性和多态性,使用g u b b i n s进行重组分析,并构建了副溶血弧菌分子血清型预测数据库。结果O10:K4的O抗原基因簇(OL10V1)与传统O10血清型基因簇(OL10)序列差异显著,而与OL4最为相似。公共数据库中116个OL10V1全长序列仅存在1个单核苷酸突变。OL10V1携带的IS1634和ISVa2转座酶基因广泛分布于弧菌属,提示该变异体可能通过水平基因转移形成。结论本研究揭示了O10:K4血清型快速传播的潜在分子机制,构建的分子血清型数据库可为基因组监测和溯源提供关键技术支撑。展开更多
Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V.parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,a...Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V.parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in Asia.However,the relationship between this serotype and pathogenicity has yet to be established.The waaf gene is located in the O antigen synthesis gene cluster.Thus,we constructed a waaf gene deletion mutant(i.e.,Δwaaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology.We then constructed theΔwaaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17λpir strain by conjugation.The basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were compared.Growth curves showed no remarkable differences between the WT andΔwaaf strains.However,theΔwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V.parahaemolyticus.Moreover,the number of flgella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity weakened.These characteristics of the C-△waaf strain were similar to those of the WT strain These results indicated that the waaf gene is vital to the serotype in V.parahaemolyticus,and changes in O antigen could affect the pathogenicity of this bacterium.This study will be helpful to understand the pathogenic mechanism of V.parahaemolyticus.展开更多
In the continuation of our study on the Serratta marcescens O-antigen, two disaccharides8 and 13, corresponding to the key intermediates of oligosaccharides related to the S. marcescensO10 antigen, were srythesized by...In the continuation of our study on the Serratta marcescens O-antigen, two disaccharides8 and 13, corresponding to the key intermediates of oligosaccharides related to the S. marcescensO10 antigen, were srythesized by the trichloroacetate method. Their structures and compositionswere characterized by H NMR, C NMR, 2D NMR and IR, FAB-MS spectra.展开更多
腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原...腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原表位可能是108-111,181-186,217-218,234-238,244-255,283-287。这为腰果过敏原的进一步研究提供理论参考,并对开发低过敏腰果制品提供帮助。展开更多
文摘目的分析副溶血弧菌O10:K4血清型O抗原基因簇的分子特征,并构建基于基因组的分子血清型预测数据库。方法从美国国家生物技术信息中心Assembly数据库获取副溶血弧菌O抗原基因簇序列,使用blastn、fastANI、Sourmash和p i x y工具分析序列相似性和多态性,使用g u b b i n s进行重组分析,并构建了副溶血弧菌分子血清型预测数据库。结果O10:K4的O抗原基因簇(OL10V1)与传统O10血清型基因簇(OL10)序列差异显著,而与OL4最为相似。公共数据库中116个OL10V1全长序列仅存在1个单核苷酸突变。OL10V1携带的IS1634和ISVa2转座酶基因广泛分布于弧菌属,提示该变异体可能通过水平基因转移形成。结论本研究揭示了O10:K4血清型快速传播的潜在分子机制,构建的分子血清型数据库可为基因组监测和溯源提供关键技术支撑。
基金supported by grants from the Natural Science Foundation of Chongqing(cstc2020jcyj-msxm X0685)the Science and Technology Research Program of Chongqing Municipal Education Commission(KJQN202001231)+1 种基金the National Natural Science Foundation of China(NSFC)(No.31201372)Science and Technology Project of Wanzhou in 2020。
文摘Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V.parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in Asia.However,the relationship between this serotype and pathogenicity has yet to be established.The waaf gene is located in the O antigen synthesis gene cluster.Thus,we constructed a waaf gene deletion mutant(i.e.,Δwaaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology.We then constructed theΔwaaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17λpir strain by conjugation.The basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were compared.Growth curves showed no remarkable differences between the WT andΔwaaf strains.However,theΔwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of V.parahaemolyticus.Moreover,the number of flgella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity weakened.These characteristics of the C-△waaf strain were similar to those of the WT strain These results indicated that the waaf gene is vital to the serotype in V.parahaemolyticus,and changes in O antigen could affect the pathogenicity of this bacterium.This study will be helpful to understand the pathogenic mechanism of V.parahaemolyticus.
文摘In the continuation of our study on the Serratta marcescens O-antigen, two disaccharides8 and 13, corresponding to the key intermediates of oligosaccharides related to the S. marcescensO10 antigen, were srythesized by the trichloroacetate method. Their structures and compositionswere characterized by H NMR, C NMR, 2D NMR and IR, FAB-MS spectra.
基金国家自然科学基金项目(30871752,30570421)The International Science and Technology(ISAT)Linkages Fund,Royal Soci-ety of New Zealand(ISATB09-33)+1 种基金广东省科技重点专项(2003A3080502)教育部留学回国人员科研启动基金资助项目
文摘腰果是引起人们过敏的主要食物之一。作者采用生物信息学方法,通过Pubmed网络服务器、生物信息分析软件SOPMA、swiss-model网络服务器、DNAStar生物分析软件等对腰果主要过敏原Ana o 2的结构和抗原表位进行预测,分析Ana o 2蛋白的抗原表位可能是108-111,181-186,217-218,234-238,244-255,283-287。这为腰果过敏原的进一步研究提供理论参考,并对开发低过敏腰果制品提供帮助。
