Carboxylated silica nanoparticles(COOH-SiO_(2) NPs)were combined with Pueraria lobata oligopeptide(POP)through ultrasonic treatment to form a protein corona(PC)on the SiO_(2) NP surface.The structural features of the ...Carboxylated silica nanoparticles(COOH-SiO_(2) NPs)were combined with Pueraria lobata oligopeptide(POP)through ultrasonic treatment to form a protein corona(PC)on the SiO_(2) NP surface.The structural features of the resulting P.lobata oligopeptide-silica nanoparticle protein corona complexes(POP-SiO_(2) PCs)were analyzed by transmission electron microscopy,particle sizing,zeta potential,and UV-visible spectrometry,as well as by infrared,fluorescence,circular dichroism,and X-ray photoelectron spectroscopies.Binding site models were established using molecular docking techniques,and the major binding forces were determined.The antioxidant activity of the POP-SiO_(2) PCs was evaluated based on their DPPH and ABTS radical-scavenging capacities.The potential use of POP-SiO_(2) PCs as food additives was assessed by their foaming ability,water-holding capacity,and emulsification properties.The DPPH and ABTS radical-scavenging rates for POP-SiO_(2) PCs reached 42.57% and 60.88%,respectively,the foaming capacity and foam stability reached 118.36% and 21.58%,respectively,the water-holding capacity and oil-holding capacity reached 452.36% and 551.71%,respectively,and the emulsion activity and emulsion stability reached 17.13 m^(2)/g and 65.27%,respectively.These findings suggest a new direction for research into NP-PC systems and offer a theoretical basis for their development and utilization in the field of food additives.展开更多
基金supported by the National Key Research and Development Program of China(No.2023YFC2604903)the National Natural Science Foundation of China(No.31201376 and 32372417)+1 种基金the Natural Science Foundation of Heilongjiang Province of China(No.LH2022C046)the Heilongjiang Leading Talent Support Program(No.2020376).
文摘Carboxylated silica nanoparticles(COOH-SiO_(2) NPs)were combined with Pueraria lobata oligopeptide(POP)through ultrasonic treatment to form a protein corona(PC)on the SiO_(2) NP surface.The structural features of the resulting P.lobata oligopeptide-silica nanoparticle protein corona complexes(POP-SiO_(2) PCs)were analyzed by transmission electron microscopy,particle sizing,zeta potential,and UV-visible spectrometry,as well as by infrared,fluorescence,circular dichroism,and X-ray photoelectron spectroscopies.Binding site models were established using molecular docking techniques,and the major binding forces were determined.The antioxidant activity of the POP-SiO_(2) PCs was evaluated based on their DPPH and ABTS radical-scavenging capacities.The potential use of POP-SiO_(2) PCs as food additives was assessed by their foaming ability,water-holding capacity,and emulsification properties.The DPPH and ABTS radical-scavenging rates for POP-SiO_(2) PCs reached 42.57% and 60.88%,respectively,the foaming capacity and foam stability reached 118.36% and 21.58%,respectively,the water-holding capacity and oil-holding capacity reached 452.36% and 551.71%,respectively,and the emulsion activity and emulsion stability reached 17.13 m^(2)/g and 65.27%,respectively.These findings suggest a new direction for research into NP-PC systems and offer a theoretical basis for their development and utilization in the field of food additives.
