Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase ...Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase contrast(DPC),a non-interferometric QPI technique,requires only four intensity images under asymmetric illumination to recover the phase of a sample,offering the advantages of being label-free,non-coherent and highly robust.Its phase reconstruction result relies on precise modeling of the phase transfer function(PTF).However,in real optical systems,the PTF will deviate from its theoretical ideal due to the unknown wavefront aberrations,which will lead to significant artifacts and distortions in the reconstructed phase.We propose an aberration-corrected DPC(ACDPC)method that utilizes three intensity images under annular illumination to jointly retrieve the aberration and the phase,achieving high-quality QPI with minimal raw data.By employing three annular illuminations precisely matched to the numerical aperture of the objective lens,the object information is transmitted into the acquired intensity with a high signal-to-noise ratio.Phase retrieval is achieved by an iterative deconvolution algorithm that uses simulated annealing to estimate the aberration and further employs regularized deconvolution to reconstruct the phase,ultimately obtaining a refined complex pupil function and an aberration-corrected quantitative phase.We demonstrate that ACDPC is robust to multi-order aberrations without any priori knowledge,and can effectively retrieve and correct system aberrations to obtain high-quality quantitative phase.Experimental results show that ACDPC can clearly reproduce subcellular structures such as vesicles and lipid droplets with higher resolution than conventional DPC,which opens up new possibilities for more accurate subcellular structure analysis in cell biology.展开更多
Obtaining residual stress is crucial for controlling the machining deformation in annular parts,and can directly influence the performance and stability of key components in advanced equipment.Since existing research ...Obtaining residual stress is crucial for controlling the machining deformation in annular parts,and can directly influence the performance and stability of key components in advanced equipment.Since existing research has achieved global residual stress field inference for components by using the deformation force-based method where the deformation force is monitored during the machining process,reliable acquisition of deformation force stll remains a significant challenge under complex machining conditions.This paper proposes a hierarchical optimization method for the layout of deformation force monitoring of annular parts.The proposed method establishes two optimization objectives by analyzing the relationship between the deformation force and the residual stress in annular parts,i.e.,equivalence and ilconditioning of solving process.Specifically,the equivalence of the monitored deformation force and residual stress in terms of effect on caused machining deformation is evaluated by local deformation,and the illconditioning is also optimized to enhance the stability of residual stress inference.Verification is implemented in both simulation and actual machining experiments,demonstrating effectiveness of the proposed layout optimization method in inferring residual stress field of annular parts with deformation force.展开更多
In concentric annular pipes,the difference in curvature between the inner and outer wall surfaces creates significant variations in the heat transfer characteristics of the two surfaces.The simplifications of the Ditt...In concentric annular pipes,the difference in curvature between the inner and outer wall surfaces creates significant variations in the heat transfer characteristics of the two surfaces.The simplifications of the Dittus-Boelter equation for circular pipes make it unsuitable for the complex flow induced by the geometry and heat transfer coupling effects in annular pipes.This prevents it from accurately predicting the turbulent heat transfer in concentric annular pipes.This paper used realizableκ–εand low Reynolds number models to conduct numerical simulations of turbulent convective heat transfer in concentric annular pipes and circular pipes.The results indicated that the local heat transfer coefficient and Nusselt number of the inner wall surface of the annular pipe were both higher than those of the outer wall surface.The Darcy resistance coefficient decreased upon increasing the Reynolds number and increased with the inner diameter-to-outer diameter ratio.When using the equivalent diameter as the characteristic scale,the turbulent heat transfer correlation obtained from circular pipes produced significant errors when used to approximate the turbulent convective heat transfer in concentric annular pipes.This error was greater for the inner wall surface,especially when the inner and outer diameters were relatively small,as the Nusselt number error on the inner wall surface reached 60.62%.The error of the Nusselt number on the outer wall surface reached 19.51%.展开更多
Installing annular wave-energy converters(WECs)on the columns of floating wind platforms in the form of a coaxial-cylinder provides a convenient means of integration.Extant coaxial-cylinder-type wind-wave hybrid syste...Installing annular wave-energy converters(WECs)on the columns of floating wind platforms in the form of a coaxial-cylinder provides a convenient means of integration.Extant coaxial-cylinder-type wind-wave hybrid systems are mostly based on single-column platforms such as spars(single coaxial-cylinder hybrid system'hereafter).Systems based on multiple-column platforms such as semi-submersible platforms('multiple coaxial-cylinder hybrid systems'hereafter)are rarely seen or studied,despite their superiority in wave-power absorption due to the use of multiple WECs as well as in dynamic stability.This paper proposes a novel WindFloat-annular-WEC hybrid system,based on our study investigating its dynamic and power features,and optimizing the geometry and power take-off of the WECs.Our results show that the dynamic and power features of a multiple coaxial-cylinder hybrid system are different from those of a single coaxial-cylinder hybrid system,so the same optimization parameters cannot be directly applied.Flatter annular WECs absorb slightly more power in a wider wave-period range,but their geometry is confined by limitations in installation and structural strength.The overall effect of an oblique incident wave is greater intensity in the motions of the hybrid system in yaw and the direction perpendicular to propagation,although the difference is small and maybe negligible.展开更多
基金supported by the National Natural Science Foundation of China(62305162,62227818,62361136588)China Postdoctoral Science Foundation(2023TQ0160,2023M731683)+5 种基金Nanjing University of Science and Technology independent research project(30923010305)National Key Research and Development Program of China(2024YFE0101300)Biomedical Competition Foundation of Jiangsu Province(BE2022847)Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039)Fundamental Research Funds for the Central Universities(2023102001)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201,JSGPCXZNGZ202401)。
文摘Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase contrast(DPC),a non-interferometric QPI technique,requires only four intensity images under asymmetric illumination to recover the phase of a sample,offering the advantages of being label-free,non-coherent and highly robust.Its phase reconstruction result relies on precise modeling of the phase transfer function(PTF).However,in real optical systems,the PTF will deviate from its theoretical ideal due to the unknown wavefront aberrations,which will lead to significant artifacts and distortions in the reconstructed phase.We propose an aberration-corrected DPC(ACDPC)method that utilizes three intensity images under annular illumination to jointly retrieve the aberration and the phase,achieving high-quality QPI with minimal raw data.By employing three annular illuminations precisely matched to the numerical aperture of the objective lens,the object information is transmitted into the acquired intensity with a high signal-to-noise ratio.Phase retrieval is achieved by an iterative deconvolution algorithm that uses simulated annealing to estimate the aberration and further employs regularized deconvolution to reconstruct the phase,ultimately obtaining a refined complex pupil function and an aberration-corrected quantitative phase.We demonstrate that ACDPC is robust to multi-order aberrations without any priori knowledge,and can effectively retrieve and correct system aberrations to obtain high-quality quantitative phase.Experimental results show that ACDPC can clearly reproduce subcellular structures such as vesicles and lipid droplets with higher resolution than conventional DPC,which opens up new possibilities for more accurate subcellular structure analysis in cell biology.
基金supported in part by the General Program of the National Natural Science Foundation of China(No.52175467)the National Key R&D Program of China(No.2022YFB3402600).
文摘Obtaining residual stress is crucial for controlling the machining deformation in annular parts,and can directly influence the performance and stability of key components in advanced equipment.Since existing research has achieved global residual stress field inference for components by using the deformation force-based method where the deformation force is monitored during the machining process,reliable acquisition of deformation force stll remains a significant challenge under complex machining conditions.This paper proposes a hierarchical optimization method for the layout of deformation force monitoring of annular parts.The proposed method establishes two optimization objectives by analyzing the relationship between the deformation force and the residual stress in annular parts,i.e.,equivalence and ilconditioning of solving process.Specifically,the equivalence of the monitored deformation force and residual stress in terms of effect on caused machining deformation is evaluated by local deformation,and the illconditioning is also optimized to enhance the stability of residual stress inference.Verification is implemented in both simulation and actual machining experiments,demonstrating effectiveness of the proposed layout optimization method in inferring residual stress field of annular parts with deformation force.
基金Supported by the Major Program of the National Natural Science Foundation of China(Grant No.51736007).
文摘In concentric annular pipes,the difference in curvature between the inner and outer wall surfaces creates significant variations in the heat transfer characteristics of the two surfaces.The simplifications of the Dittus-Boelter equation for circular pipes make it unsuitable for the complex flow induced by the geometry and heat transfer coupling effects in annular pipes.This prevents it from accurately predicting the turbulent heat transfer in concentric annular pipes.This paper used realizableκ–εand low Reynolds number models to conduct numerical simulations of turbulent convective heat transfer in concentric annular pipes and circular pipes.The results indicated that the local heat transfer coefficient and Nusselt number of the inner wall surface of the annular pipe were both higher than those of the outer wall surface.The Darcy resistance coefficient decreased upon increasing the Reynolds number and increased with the inner diameter-to-outer diameter ratio.When using the equivalent diameter as the characteristic scale,the turbulent heat transfer correlation obtained from circular pipes produced significant errors when used to approximate the turbulent convective heat transfer in concentric annular pipes.This error was greater for the inner wall surface,especially when the inner and outer diameters were relatively small,as the Nusselt number error on the inner wall surface reached 60.62%.The error of the Nusselt number on the outer wall surface reached 19.51%.
基金supported by the National Natural Science Foundation of China(Nos.52201322,52222109,and 52071096)the Guangdong Basic and Applied Basic Research Foundation(Nos.2022B1515020036 and 2023A1515012144)the Natural Science Foundation of Guangzhou City(No.202201010055),China.
文摘Installing annular wave-energy converters(WECs)on the columns of floating wind platforms in the form of a coaxial-cylinder provides a convenient means of integration.Extant coaxial-cylinder-type wind-wave hybrid systems are mostly based on single-column platforms such as spars(single coaxial-cylinder hybrid system'hereafter).Systems based on multiple-column platforms such as semi-submersible platforms('multiple coaxial-cylinder hybrid systems'hereafter)are rarely seen or studied,despite their superiority in wave-power absorption due to the use of multiple WECs as well as in dynamic stability.This paper proposes a novel WindFloat-annular-WEC hybrid system,based on our study investigating its dynamic and power features,and optimizing the geometry and power take-off of the WECs.Our results show that the dynamic and power features of a multiple coaxial-cylinder hybrid system are different from those of a single coaxial-cylinder hybrid system,so the same optimization parameters cannot be directly applied.Flatter annular WECs absorb slightly more power in a wider wave-period range,but their geometry is confined by limitations in installation and structural strength.The overall effect of an oblique incident wave is greater intensity in the motions of the hybrid system in yaw and the direction perpendicular to propagation,although the difference is small and maybe negligible.
