The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the...The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the position of the animation art has been promoted by the expression and extension of the drama art spirit and expression technique in the creation of film and television animations to be greatly improved. In this paper, the characteristics of the virtual technique in drama are briefly introduced, and also the influence of the virtual technique on the creation offihn and television animations is analyzed.展开更多
The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educat...The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educational programming environment of which feature is an intuitively comprehensive user interface in terms of state transition diagrams. Unfortunately its animation definition was too memory-hungry when played by the built-in interpreter. In this paper, we present a translator of the animation definitions of Islay 3D into ActionScript3 (AS3). Compiling the AS3 codes by way of Papervision3D, we obtain the 3D Flash animation file playable on the Flash platform. It will be shown that the memory and CPU usages will be much saved, quartered and halved, respectively, by virtue of the translator.展开更多
Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition...Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition to standardization and reproducibility, virtual stimuli open new options for re-searchers since they are easily changeable in morphology and appearance, and their behavior canbe defined. In this article, a novel toolchain to conduct behavior experiments with fish is presentedby a case study in sailfin mollies Poecilia latipinna. As the toolchain holds many different and novelfeatures, it offers new possibilities for studies in behavioral animal research and promotes thestandardization of experiments. The presented method includes options to design, animate, andpresent virtual stimuli to live fish. The designing tool offers an easy and user-friendly way to definesize, coloration, and morphology of stimuli and moreover it is able to configure virtual stimuli ran-domly without any user influence. Furthermore, the toolchain brings a novel method to animatestimuli in a semiautomatic way with the help of a game controller. These created swimming pathscan be applied to different stimuli in real time. A presentation tool combines models and swim-ming paths regarding formerly defined playlists, and presents the stimuli onto 2 screens.Experiments with live sailfin mollies validated the usage of the created virtual 3D fish models inmate-choice experiments.展开更多
Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or mo...Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or motivation. Communication of aggressive propensity is particularly importantin group-living animals with a stable dominance hierarchy, as the outcome of aggressive inter-actions determines the social rank of group members. Neolamprologus pulcher is a cooperativelybreeding cichlid showing frequent within-group aggression. Both sexes exhibit two vertical blackstripes on the operculum that vary naturally in shape and darkness. During frontal threat displaysthese patterns are actively exposed to the opponent, suggesting a signaling function. To investi-gate the role of operculum stripes during contests we manipulated their darkness in computeranimated pictures of the fish. We recorded the responses in behavior and stripe darkness of testsubjects to which these animated pictures were presented. Individuals with initially darker stripeswere more aggressive against the animations and showed more operculum threat displays.Operculum stripes of test subjects became darker after exposure to an animation exhibiting a paleoperculum than after exposure to a dark operculum animation, highlighting the role of the dark-ness of this color pattern in opponent assessment. We conclude that （i） the black stripes on theoperculum of N. pulcherare a reliable signal of aggression and dominance, （ii） these markings playan important role in opponent assessment, and （iii） 2D computer animations are well suited to elicitbiologically meaningful short-term aggressive responses in this widely used model system ofsocial evolution.展开更多
The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation ...The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation of animated animal stimuli with high variability of morphology and even behavior; （2）animated stimuli provide highly standardized, controlled and repeatable testing procedures; and （3）they allow a reduction in the number of live test animals regarding the 3Rs principle. But the use of ani-mated animals should be attended by a thorough validation for each test species to verify that behaviormeasured with live animals toward virtual animals can also be expected with natural stimuli. Here wepresent results on the validation of a custom-made simulation for animated 3D sailfin mollies Poecilialatipinna and show that responses of live test females were as strong to an animated fish as to a videoor a live male fish. Movement of an animated stimulus was important but female response was stron-ger toward a swimming 3D fish stimulus than to a ＂swimming＂ box. Moreover, male test fish wereable to discriminate between animated male and female stimuli; hence, rendering the animated 3D fisha useful tool in mate-choice experiments with sailfin mollies.展开更多
The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(W...The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(Witte et al.2017).The importance of easy-tomanipulate,consistent,and reliably performing stimuli is clear,and this is readily achievable with the use of computer animations.Validation of animated stimuli is critical,however,to ensure that behaviors observed in response to artificial,computer-generated stimuli are equivalent to what would be expected if animals were presented with a comparable live stimuli(Woo and Rieucau 2011;Chouinard-Thuly et al.2017;Powell and Rosenthal 2017).展开更多
Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China&#...Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China's stories well?In Shandong Province,documentaries and animations have been used to break cultural barriers and open new horizons in city-fo cused international communication.展开更多
Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microgl...Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate do...A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate dosimetry is a crucial component of small animal and preclinical irradiation studies.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this com...Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this complex problem.One such model,the Oncopig,has been reported to develop tumors of up to 4 cm in diameter within 7-14 days at sites of in situ vector inoculation.However,the resulting lesions reportedly contained an extensive inflammatory component that has not been evaluated in detail.Methods:Herein,we describe our results from multiparametric characterization of the lesions generated using liver biopsy cores incubated in vector solution and re-placed in the tissue.The study consisted of 3 animals in 3 cohorts(total of 9 animals)that were evaluated at 14,21,and 28 days.CT imaging,immunohistochemistry,multiplex immunofluorescence,and comprehensive blood analyses were used to quantify composition of the hepatic masses that developed following AdCre inoculation.Results:The tumors were hypovascular on CT and predominantly composed of CD45+cells with a strong lymphohistiocytic component,with no carcinomas identified.Ki-67 staining showed proliferation of CD45+immune cells but no neoplastic component.To provide further insight,the results are evaluated in the context of tumor growth kinetics.Conclusion:While progress has been made in generating targetable lesions,achieving a robust large animal model of liver cancer that faithfully recapitulates the human disease remains a challenging goal.展开更多
On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experime...On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experimental Medicine(AMEM).Over the past year,we have faced challenges together and achieved breakthroughs in academic influence,internationalization,and fulfilling social respon-sibilities.Looking ahead,we are filled with confidence as we strive to build an important bridge connecting laboratory animal science and technology with academic research.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.The research scope includes the quality and processing characteristics of food raw materials,the relationships of nutritional components and bioactive substances with human health,product flavor and sensory characteristics,the control of harmful substances during processing or cooking,product preservation,storage and packaging;microorganisms and fermentation,illegal drug residues and food safety detection;authenticity identification;cell-cultured meat,regulations and standards.展开更多
Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecul...Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.展开更多
Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male m...Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male macaques aged 10-15 years underwent an 18-month HFD intervention.Physiological parameters(BMI,BP,hematology),liver fat fraction(evaluated by ultrasound/MRI),cardiac function(assessed by echocardiography),and histopathology(using liver biopsy)were measured before and after the intervention.Serum proteomics with KEGG/STRING analyses identified molecular mechanisms.Results:Within 6 months,HFD induced dyslipidemia(elevated TG,TCHO,HDL-C,LDL-C).After 18 months,metabolic dysfunction-associated steatohepatitis(MASH)was confirmed by histopathology in 57.14%(16/28)of macaques,diabetes(elevated FPG/HbA1c)in 17.86%(5/28),and myocardial hypertrophy(elevated LVMass/LAD)in 46.43%(13/28).Proteomics identified Bile acid-CoA:amino acid N-acyltransferase(BAAT)as a MASH hallmark protein,the level of which was inversely correlated with the degree of fibrosis.For diabetes,citrate synthase(CS)and malate dehydrogenase 1(MDH1)impaired glucose oxidation via the TCA cycle,while hexose-6-phosphate de-hydrogenase(H6PD)disrupted gluconeogenesis.Myocardial hypertrophy was associ-ated with the downregulation of SRC proto-oncogene,non-receptor tyrosine kinase(SRC),mitogen-activated protein kinase 14(MAPK14),emerin(EMD),and integrin subunit beta 1(ITGB1).Conclusions:An 18-month HFD successfully established a translational M.fascicula-ris model replicating key metabolic disorders(MASH,diabetes,cardiac hypertrophy).BAAT,CS/MDH1/H6PD,and SRC/MAPK14/EMD/ITGB1 were identified as mecha-nistic biomarkers for these conditions.展开更多
Background:This study aims to explore the establishment of an animal model of car-diac injury induced by trimethylamine-N-oxide(TMAO),a metabolite secreted by gut microorganisms,and to investigate its application in m...Background:This study aims to explore the establishment of an animal model of car-diac injury induced by trimethylamine-N-oxide(TMAO),a metabolite secreted by gut microorganisms,and to investigate its application in moderate-intensity continuous training(MICT)intervention.Methods:C57BL6/J mice were randomly divided into four groups:normal mice(Nor,n=15);mice administered TMAO(TMAO,n=15);mice undergoing(Nor+MICT,n=15);mice undergoing(MICT)and administered TMAO(TMAO+MICT,n=15).Mice in the TMAO and TMAO+MICT groups received daily gavage of high-dose TMAO for 8 weeks,whereas those in the Nor+MICT and TMAO+MICT groups underwent MICT for 8 weeks(60 min per session,5 days per week,at 50%maximal running capacity).Cardiac function was evaluated using ultrasound,myocardial histology was examined using hematoxylin and eosin(HE)staining,and nuclear magnetic resonance(NMR)-based metabolomics was employed for multivariate statistical and metabolic pathway analyses.Results:Relative to the Nor group,TMAO-treated mice exhibited significant weight loss,elevated heart rate,and reduced ejection fraction and left ventricular fractional shortening,indicating cardiac impairment.Importantly,the TMAO+MICT group dem-onstrated significant improvements in these parameters compared to the TMAO group,alongside distinct alterations in myocardial metabolic profiles.TMAO altered five metabolic pathways relative to controls,whereas MICT induced significant changes in three pathways in TMAO-treated mice.Conclusion:Eight weeks of high-dose TMAO administration induced significant cardiac dysfunction in mice,which was effectively mitigated by MICT intervention.Consequently,this animal model serves as a valuable tool for investigating the mecha-nisms underlying the impact of MICT on cardiovascular diseases.展开更多
Background:The traditional method of heterotopic abdominal heart transplantation(HTx)involves crossclamping the inferior vena cava,which inevitably leads to bilateral lower limb ischemia(LI).This study first aimed to ...Background:The traditional method of heterotopic abdominal heart transplantation(HTx)involves crossclamping the inferior vena cava,which inevitably leads to bilateral lower limb ischemia(LI).This study first aimed to investigate the impact of LI on renal function in rats subjected to unilateral nephrectomy(UNx).Second,a modified method utilizing renal vessel-assisted anastomosis in rats with left UNx was compared with the traditional method for abdominal HTx.Methods:Male Sprague-Dawley rats were utilized as subjects for both experimental phases.In experiment 1,the animals were divided into four groups:sham operation group;LI group-rats undergoing occlusion of the abdominal aorta and vena cava below the renal vessels;UNx group-rats with left UNx;and LI+UNx group.All operated animals were monitored for up to 7 days for biochemical markers,renal histopathology,and survival rates.In experiment 2,we introduced the renal vessel-assisted method as the experimental group and compared it against the traditional method as the control within rat heterotopic HTx models.We assessed operative characteristics,echocardiography results,histological findings,and graft survival.Results:First,LI resulted in acute kidney dysfunction characterized by a decrease in 7day survival rates and creatinine clearance rates in both the LI and LI+UNx groups compared to the sham operation and UNx groups.Particularly,histopathological damage in the kidney and liver did not exhibit significant effects during this period.Second,the implementation of the renal vessel-assisted method significantly reduced bleeding volume at suture sites and enhanced the 7day survival rate compared to the traditional method.Conclusion:Acute kidney injury was induced by LI postoperation in treated rats.The renal vessel-assisted method demonstrated its effectiveness as a superior alternative that mitigates complications associated with the traditional method.展开更多
Confucius’imminent birth is heralded by the appearance of the qilin.The mythical one-horned animal came to his mother at the door and cast out of its mouth a jade tablet bearing an inscription saying that she would g...Confucius’imminent birth is heralded by the appearance of the qilin.The mythical one-horned animal came to his mother at the door and cast out of its mouth a jade tablet bearing an inscription saying that she would give birth to“the son of the refinement of water,and that he would succeed the Zhou Dynasty,but as a king without a throne(su wang).”Stunned,Yan Zhengzai–Confucius’mother–tied an embroidered ribbon around the horn of the qilin,and the animal stayed for two nights.展开更多
文摘The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the position of the animation art has been promoted by the expression and extension of the drama art spirit and expression technique in the creation of film and television animations to be greatly improved. In this paper, the characteristics of the virtual technique in drama are briefly introduced, and also the influence of the virtual technique on the creation offihn and television animations is analyzed.
文摘The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educational programming environment of which feature is an intuitively comprehensive user interface in terms of state transition diagrams. Unfortunately its animation definition was too memory-hungry when played by the built-in interpreter. In this paper, we present a translator of the animation definitions of Islay 3D into ActionScript3 (AS3). Compiling the AS3 codes by way of Papervision3D, we obtain the 3D Flash animation file playable on the Flash platform. It will be shown that the memory and CPU usages will be much saved, quartered and halved, respectively, by virtue of the translator.
文摘Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition to standardization and reproducibility, virtual stimuli open new options for re-searchers since they are easily changeable in morphology and appearance, and their behavior canbe defined. In this article, a novel toolchain to conduct behavior experiments with fish is presentedby a case study in sailfin mollies Poecilia latipinna. As the toolchain holds many different and novelfeatures, it offers new possibilities for studies in behavioral animal research and promotes thestandardization of experiments. The presented method includes options to design, animate, andpresent virtual stimuli to live fish. The designing tool offers an easy and user-friendly way to definesize, coloration, and morphology of stimuli and moreover it is able to configure virtual stimuli ran-domly without any user influence. Furthermore, the toolchain brings a novel method to animatestimuli in a semiautomatic way with the help of a game controller. These created swimming pathscan be applied to different stimuli in real time. A presentation tool combines models and swim-ming paths regarding formerly defined playlists, and presents the stimuli onto 2 screens.Experiments with live sailfin mollies validated the usage of the created virtual 3D fish models inmate-choice experiments.
文摘Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or motivation. Communication of aggressive propensity is particularly importantin group-living animals with a stable dominance hierarchy, as the outcome of aggressive inter-actions determines the social rank of group members. Neolamprologus pulcher is a cooperativelybreeding cichlid showing frequent within-group aggression. Both sexes exhibit two vertical blackstripes on the operculum that vary naturally in shape and darkness. During frontal threat displaysthese patterns are actively exposed to the opponent, suggesting a signaling function. To investi-gate the role of operculum stripes during contests we manipulated their darkness in computeranimated pictures of the fish. We recorded the responses in behavior and stripe darkness of testsubjects to which these animated pictures were presented. Individuals with initially darker stripeswere more aggressive against the animations and showed more operculum threat displays.Operculum stripes of test subjects became darker after exposure to an animation exhibiting a paleoperculum than after exposure to a dark operculum animation, highlighting the role of the dark-ness of this color pattern in opponent assessment. We conclude that （i） the black stripes on theoperculum of N. pulcherare a reliable signal of aggression and dominance, （ii） these markings playan important role in opponent assessment, and （iii） 2D computer animations are well suited to elicitbiologically meaningful short-term aggressive responses in this widely used model system ofsocial evolution.
文摘The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation of animated animal stimuli with high variability of morphology and even behavior; （2）animated stimuli provide highly standardized, controlled and repeatable testing procedures; and （3）they allow a reduction in the number of live test animals regarding the 3Rs principle. But the use of ani-mated animals should be attended by a thorough validation for each test species to verify that behaviormeasured with live animals toward virtual animals can also be expected with natural stimuli. Here wepresent results on the validation of a custom-made simulation for animated 3D sailfin mollies Poecilialatipinna and show that responses of live test females were as strong to an animated fish as to a videoor a live male fish. Movement of an animated stimulus was important but female response was stron-ger toward a swimming 3D fish stimulus than to a ＂swimming＂ box. Moreover, male test fish wereable to discriminate between animated male and female stimuli; hence, rendering the animated 3D fisha useful tool in mate-choice experiments with sailfin mollies.
文摘The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(Witte et al.2017).The importance of easy-tomanipulate,consistent,and reliably performing stimuli is clear,and this is readily achievable with the use of computer animations.Validation of animated stimuli is critical,however,to ensure that behaviors observed in response to artificial,computer-generated stimuli are equivalent to what would be expected if animals were presented with a comparable live stimuli(Woo and Rieucau 2011;Chouinard-Thuly et al.2017;Powell and Rosenthal 2017).
文摘Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China's stories well?In Shandong Province,documentaries and animations have been used to break cultural barriers and open new horizons in city-fo cused international communication.
基金supported by the Natural Science Foundation of Yunnan Province,No.202401AS070086(to ZW)the National Key Research and Development Program of China,No.2018YFA0801403(to ZW)+1 种基金Yunnan Science and Technology Talent and Platform Plan,No.202105AC160041(to ZW)the Natural Science Foundation of China,No.31960120(to ZW)。
文摘Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
文摘A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate dosimetry is a crucial component of small animal and preclinical irradiation studies.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
基金Institutional Research Grant,MD Anderson Cancer CenterUPWARDS Training Program(Undergraduate Students Working Towards Research in Science),Grant/Award Number:1R25CA240137-01A1the CPRIT Research Training Award CPRIT Training Program,Grant/Award Number:RP210028。
文摘Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this complex problem.One such model,the Oncopig,has been reported to develop tumors of up to 4 cm in diameter within 7-14 days at sites of in situ vector inoculation.However,the resulting lesions reportedly contained an extensive inflammatory component that has not been evaluated in detail.Methods:Herein,we describe our results from multiparametric characterization of the lesions generated using liver biopsy cores incubated in vector solution and re-placed in the tissue.The study consisted of 3 animals in 3 cohorts(total of 9 animals)that were evaluated at 14,21,and 28 days.CT imaging,immunohistochemistry,multiplex immunofluorescence,and comprehensive blood analyses were used to quantify composition of the hepatic masses that developed following AdCre inoculation.Results:The tumors were hypovascular on CT and predominantly composed of CD45+cells with a strong lymphohistiocytic component,with no carcinomas identified.Ki-67 staining showed proliferation of CD45+immune cells but no neoplastic component.To provide further insight,the results are evaluated in the context of tumor growth kinetics.Conclusion:While progress has been made in generating targetable lesions,achieving a robust large animal model of liver cancer that faithfully recapitulates the human disease remains a challenging goal.
文摘On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experimental Medicine(AMEM).Over the past year,we have faced challenges together and achieved breakthroughs in academic influence,internationalization,and fulfilling social respon-sibilities.Looking ahead,we are filled with confidence as we strive to build an important bridge connecting laboratory animal science and technology with academic research.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.The research scope includes the quality and processing characteristics of food raw materials,the relationships of nutritional components and bioactive substances with human health,product flavor and sensory characteristics,the control of harmful substances during processing or cooking,product preservation,storage and packaging;microorganisms and fermentation,illegal drug residues and food safety detection;authenticity identification;cell-cultured meat,regulations and standards.
基金Ministry of Research,Innovation and Digitization,CCCDI-UEFISCDI,Grant/Award Number:PN-IV-P7-7.1-PED-2024-1578,within PNCDI Ⅳ.
文摘Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.
基金National Key Research and Development Program of China,Grant/Award Number:2021YFF0702200Science and Technology Projects in Guangzhou,Grant/Award Number:202206010084,202206010197 and 202206060002+1 种基金Guangdong S&T programme,Grant/Award Number:2009A081000002 and 2023B0303040004Technology Planning Project of Linzhi,Grant/Award Number:2023-YZ-01。
文摘Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male macaques aged 10-15 years underwent an 18-month HFD intervention.Physiological parameters(BMI,BP,hematology),liver fat fraction(evaluated by ultrasound/MRI),cardiac function(assessed by echocardiography),and histopathology(using liver biopsy)were measured before and after the intervention.Serum proteomics with KEGG/STRING analyses identified molecular mechanisms.Results:Within 6 months,HFD induced dyslipidemia(elevated TG,TCHO,HDL-C,LDL-C).After 18 months,metabolic dysfunction-associated steatohepatitis(MASH)was confirmed by histopathology in 57.14%(16/28)of macaques,diabetes(elevated FPG/HbA1c)in 17.86%(5/28),and myocardial hypertrophy(elevated LVMass/LAD)in 46.43%(13/28).Proteomics identified Bile acid-CoA:amino acid N-acyltransferase(BAAT)as a MASH hallmark protein,the level of which was inversely correlated with the degree of fibrosis.For diabetes,citrate synthase(CS)and malate dehydrogenase 1(MDH1)impaired glucose oxidation via the TCA cycle,while hexose-6-phosphate de-hydrogenase(H6PD)disrupted gluconeogenesis.Myocardial hypertrophy was associ-ated with the downregulation of SRC proto-oncogene,non-receptor tyrosine kinase(SRC),mitogen-activated protein kinase 14(MAPK14),emerin(EMD),and integrin subunit beta 1(ITGB1).Conclusions:An 18-month HFD successfully established a translational M.fascicula-ris model replicating key metabolic disorders(MASH,diabetes,cardiac hypertrophy).BAAT,CS/MDH1/H6PD,and SRC/MAPK14/EMD/ITGB1 were identified as mecha-nistic biomarkers for these conditions.
基金National Natural Science Foundation of China,Grant/Award Number:32271496China Fundamental Research Funds for the Central Universities(Bejing Sport University)Grant/Award Number:2024TZJK001。
文摘Background:This study aims to explore the establishment of an animal model of car-diac injury induced by trimethylamine-N-oxide(TMAO),a metabolite secreted by gut microorganisms,and to investigate its application in moderate-intensity continuous training(MICT)intervention.Methods:C57BL6/J mice were randomly divided into four groups:normal mice(Nor,n=15);mice administered TMAO(TMAO,n=15);mice undergoing(Nor+MICT,n=15);mice undergoing(MICT)and administered TMAO(TMAO+MICT,n=15).Mice in the TMAO and TMAO+MICT groups received daily gavage of high-dose TMAO for 8 weeks,whereas those in the Nor+MICT and TMAO+MICT groups underwent MICT for 8 weeks(60 min per session,5 days per week,at 50%maximal running capacity).Cardiac function was evaluated using ultrasound,myocardial histology was examined using hematoxylin and eosin(HE)staining,and nuclear magnetic resonance(NMR)-based metabolomics was employed for multivariate statistical and metabolic pathway analyses.Results:Relative to the Nor group,TMAO-treated mice exhibited significant weight loss,elevated heart rate,and reduced ejection fraction and left ventricular fractional shortening,indicating cardiac impairment.Importantly,the TMAO+MICT group dem-onstrated significant improvements in these parameters compared to the TMAO group,alongside distinct alterations in myocardial metabolic profiles.TMAO altered five metabolic pathways relative to controls,whereas MICT induced significant changes in three pathways in TMAO-treated mice.Conclusion:Eight weeks of high-dose TMAO administration induced significant cardiac dysfunction in mice,which was effectively mitigated by MICT intervention.Consequently,this animal model serves as a valuable tool for investigating the mecha-nisms underlying the impact of MICT on cardiovascular diseases.
基金The Youth Project of Tianjin Natural Science Foundation,Grant/Award Number:23JCQNJC01380。
文摘Background:The traditional method of heterotopic abdominal heart transplantation(HTx)involves crossclamping the inferior vena cava,which inevitably leads to bilateral lower limb ischemia(LI).This study first aimed to investigate the impact of LI on renal function in rats subjected to unilateral nephrectomy(UNx).Second,a modified method utilizing renal vessel-assisted anastomosis in rats with left UNx was compared with the traditional method for abdominal HTx.Methods:Male Sprague-Dawley rats were utilized as subjects for both experimental phases.In experiment 1,the animals were divided into four groups:sham operation group;LI group-rats undergoing occlusion of the abdominal aorta and vena cava below the renal vessels;UNx group-rats with left UNx;and LI+UNx group.All operated animals were monitored for up to 7 days for biochemical markers,renal histopathology,and survival rates.In experiment 2,we introduced the renal vessel-assisted method as the experimental group and compared it against the traditional method as the control within rat heterotopic HTx models.We assessed operative characteristics,echocardiography results,histological findings,and graft survival.Results:First,LI resulted in acute kidney dysfunction characterized by a decrease in 7day survival rates and creatinine clearance rates in both the LI and LI+UNx groups compared to the sham operation and UNx groups.Particularly,histopathological damage in the kidney and liver did not exhibit significant effects during this period.Second,the implementation of the renal vessel-assisted method significantly reduced bleeding volume at suture sites and enhanced the 7day survival rate compared to the traditional method.Conclusion:Acute kidney injury was induced by LI postoperation in treated rats.The renal vessel-assisted method demonstrated its effectiveness as a superior alternative that mitigates complications associated with the traditional method.
文摘Confucius’imminent birth is heralded by the appearance of the qilin.The mythical one-horned animal came to his mother at the door and cast out of its mouth a jade tablet bearing an inscription saying that she would give birth to“the son of the refinement of water,and that he would succeed the Zhou Dynasty,but as a king without a throne(su wang).”Stunned,Yan Zhengzai–Confucius’mother–tied an embroidered ribbon around the horn of the qilin,and the animal stayed for two nights.
