AIM: To investigate the expression of lacrimal androgenbinding proteins(ABPs) in mice Pseudomonas aeruginosa(P. aeruginosa) keratitis.METHODS: P. aeruginosa mice model from different gender was developed by intra-stro...AIM: To investigate the expression of lacrimal androgenbinding proteins(ABPs) in mice Pseudomonas aeruginosa(P. aeruginosa) keratitis.METHODS: P. aeruginosa mice model from different gender was developed by intra-stromal injection. The expression of lacrimal ABPs in lacrimal gland specimens from P. aeruginosa keratitis mice was detected by the quantitative polymerase chain reaction(q RT-PCR). Corneal virulence was evaluated based on clinical scores. To study the mechanism of lacrimal ABPs' expression, experimental subjects were pre-treated with 4 E-BP1 inhibitor, and were used to evaluate the expression levels by q RT-PCR.RESULTS: Compared with control groups, the expression of ABPα, ABPη and ABPζ in lacrimal gland from P. aeruginosa keratitis mice had no meaningful changes, while ABPε and ABPδ were significantly higher at 1 d after infection. The expression of ABPδ in lacrimal gland of male mice was higher than female mice, regardless of whether or not P. aeruginosa keratitis occurred. After 4 E-BP1 inhibitor subconjunctival injection or lacrimal injection, the expression of ABPδ and ABPε has no significant change compared with the control group.CONCLUSION: ABPδ and ABPε secreted by mice lacrimal gland may involve in the progress of alleviating the severity of corneal damage in P. aeruginosa keratitis. The expression of ABPδ and ABPε upon P. aeruginosa infection is independent of cap-dependent m RNA translation activated by 4 E-BP1.展开更多
Aim: To study the DHT-binding ability of benign prostatic hypertrophy (BPH) and its etiological relationship with BPH. Methods: Cytosolic and nuclear fractions was obtained from 32 BPH tissues by superpubic prostatect...Aim: To study the DHT-binding ability of benign prostatic hypertrophy (BPH) and its etiological relationship with BPH. Methods: Cytosolic and nuclear fractions was obtained from 32 BPH tissues by superpubic prostatectomy and all the endogenous hormone were removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed after the addition of 3H-DHT. Results: The average DHT-binding capacity of the BPH is 0.024 nmol (protein)/g wet tissue. The DHT-binding capacity of the cytosolic and the nuclear fractions were (0.0128±0.0020) nmol/g and (0.0112±0.0059) nmol/g wet tissue, respectively, the difference of the two capacities being insignificant (P>0.05). Conclusion: The DHT-binding capacity of BPH is high and thus facilitates the effect of DHT on BPH.展开更多
基金Supported by the National Natural Science Foundation of China(No.81300730)China Postdoctoral Science Foundation(No.2018M630482)+2 种基金Key Research Project of Shandong Province(No.2018GSF118193)Science and Technology Project of Qingdao(No.19-6-1-39-nsh)Qingdao Outstanding Health Professional Development Fund
文摘AIM: To investigate the expression of lacrimal androgenbinding proteins(ABPs) in mice Pseudomonas aeruginosa(P. aeruginosa) keratitis.METHODS: P. aeruginosa mice model from different gender was developed by intra-stromal injection. The expression of lacrimal ABPs in lacrimal gland specimens from P. aeruginosa keratitis mice was detected by the quantitative polymerase chain reaction(q RT-PCR). Corneal virulence was evaluated based on clinical scores. To study the mechanism of lacrimal ABPs' expression, experimental subjects were pre-treated with 4 E-BP1 inhibitor, and were used to evaluate the expression levels by q RT-PCR.RESULTS: Compared with control groups, the expression of ABPα, ABPη and ABPζ in lacrimal gland from P. aeruginosa keratitis mice had no meaningful changes, while ABPε and ABPδ were significantly higher at 1 d after infection. The expression of ABPδ in lacrimal gland of male mice was higher than female mice, regardless of whether or not P. aeruginosa keratitis occurred. After 4 E-BP1 inhibitor subconjunctival injection or lacrimal injection, the expression of ABPδ and ABPε has no significant change compared with the control group.CONCLUSION: ABPδ and ABPε secreted by mice lacrimal gland may involve in the progress of alleviating the severity of corneal damage in P. aeruginosa keratitis. The expression of ABPδ and ABPε upon P. aeruginosa infection is independent of cap-dependent m RNA translation activated by 4 E-BP1.
文摘Aim: To study the DHT-binding ability of benign prostatic hypertrophy (BPH) and its etiological relationship with BPH. Methods: Cytosolic and nuclear fractions was obtained from 32 BPH tissues by superpubic prostatectomy and all the endogenous hormone were removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed after the addition of 3H-DHT. Results: The average DHT-binding capacity of the BPH is 0.024 nmol (protein)/g wet tissue. The DHT-binding capacity of the cytosolic and the nuclear fractions were (0.0128±0.0020) nmol/g and (0.0112±0.0059) nmol/g wet tissue, respectively, the difference of the two capacities being insignificant (P>0.05). Conclusion: The DHT-binding capacity of BPH is high and thus facilitates the effect of DHT on BPH.
