Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly compose...Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly composed of very long-chain fatty acids(VLCFAs);their derivatives,including esters,primary alcohols,secondary alcohols,aldehydes,and ketones;and triterpenoids.This complex mixture of lipids is probably biosynthesized in the epidermal cells of most plants and exuded onto the surface.Cuticular wax not only makes the fruit less susceptible to microbial infection but also reduces mechanical damage to the fruit,thereby maintaining the fruit’s commodity value.To date,research has mostly focused on the changes,function,and regulation of fruit wax before harvest,while ignoring the changes and functions of wax in fruit storage.This paper reviews on the composition,structure,and metabolic regulation of cuticular wax in fruits.It also focuses on postharvest factors affecting wax composition,such as storage temperature,relative humidity(RH),gas atmosphere,and as exogenous hormones;and the effects of wax on fruit postharvest quality,including water dispersion,fruit softening,physiological disorders,and disease resistance.These summaries may be of assistance in better understanding the changes in cuticular wax in postharvest fruit and the resulting effects on fruit quality.展开更多
This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and arc...This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and archaeal communities varied greatly with depth.Proteobacteria in bacterial communities and Nitrososphaeria and Woesearchaeota in archaeal communities were dominant in the shallow sediments(1-40 cm),while Chloroflexi in bacterial communities and Bathyarchaeia in archaeal communities were dominant in the deep sediments(50-200 cm).Regarding ecological functions based on the metatranscriptomic data,genes involved in various pathways of nitrogen metabolism and sulfur metabolism were observed in the tested sediment samples.Metagenomic analysis revealed that Proteobacteria contribute the most to nearly all genes involved in nitrogen and sulfur metabolism.Moreover,Thaumarchaeota contribute the most to certain genes involved in nitrification,denitrification and assimilatory sulfate reduction pathways.The most abundant bacterial genus,Candidatus Scalindua,is crucial for nitrification,dissimilatory nitrate reduction,denitrification and assimilatory sulfate reduction pathways.展开更多
Ion channels,as membrane proteins,are the sensors of the cell.They act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into ...Ion channels,as membrane proteins,are the sensors of the cell.They act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into unique electrical signals to shape the responses of excitable cells.Because of their importance in cellular communication,ion channels have been intensively studied at the structural and functional levels.Here,we summarize the diverse approaches,including molecular and cellular,chemical,optical,biophysical,and computational,used to probe the structural and functional rearrangements that occur during channel activation（or sensitization）,inactivation（or desensitization）,and various forms of modulation.The emerging insights into the structure and function of ion channels by multidisciplinary approaches allow the development of new pharmacotherapies as well as new tools useful in controlling cellular activity.展开更多
As the world’s first country to launch industrial revolution,the UK had once been at the peak of global power for a long term and exerted great inf luence on the shaping of modern
To investigate the effects of aluminum trichloride （AlCl3） on renal structure and function in chickens, the chickens were injected continuously with different gradient AlCl3 for 60 days to establish sub-chronic alum...To investigate the effects of aluminum trichloride （AlCl3） on renal structure and function in chickens, the chickens were injected continuously with different gradient AlCl3 for 60 days to establish sub-chronic aluminum （Al） intoxication model. The AI concentration in serum and kidney, contents of creatinine （Cr） and blood urea nitrogen （BUN） in serum were detected, and the renal structure was also observed by optical microscope. The results showed that the A1 concentration in serum and kidney, the contents of Cr and BUN in serum were significantly higher in Al-treated chickens than those in the control group （P〈0.05; P〈0.01） and there was an AlCl3 dose-depended manner. Histopathologically, in the low dose group, acinus renis swelled mildly and there were no apparent pathological changes in nephric tubule and proximal convoluted tubule. In the middle and high dose group, acinus renis swelled, glomerular cells increased, nephric tubule cloudy swelled, and epithelial cells of proximal convoluted tubule swelled. The results indicated that sub-chronic Al exposure impaired the renal structure and function in chickens.展开更多
BACKGROUND： Ultraviolet blood irradiation and oxygenation （UBIO） has obtained better clinical effect in treating acute cerebral infarction, but the mechanism underlying this effect remains unclear. OBJECTIVE： To o...BACKGROUND： Ultraviolet blood irradiation and oxygenation （UBIO） has obtained better clinical effect in treating acute cerebral infarction, but the mechanism underlying this effect remains unclear. OBJECTIVE： To observe the effect of UBIO on the nerve function and activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase activities on the red blood cell （RBC） membrane of patients with acute cerebral infarction. DESIGN： A randomized and controlled study.SETTING： Department of Neurology, Xiangfan Central Hospital.PARTICIPANTS： From January 2000 to December 2001, excluding those above 70 years old, 58 cases of 700 patients with acute cerebral infarction admitted in the Department of Neurology, Xiangfan Central Hospital, were recruited and divided into two groups according to the random number table： UBIO treated group （n=28）, including 17 males and 11 females, aged 40-68 years; and control group （n=30）, including 20 males and 10 females, aged 44-69 years. All the patients agreed to participate in the therapeutic program and detected items. The general informations were comparable without obvious differences between the two groups （P 〉 0.05）.METHODS： ① The patients in both groups received routine treatments, besides, those in the UBIO treated group were given UBIO treatment by using the XL-200 type therapeutic apparatus produced in Shijiazhuang, whose ultraviolet wave was set at 253.7 nm with the energy density of 0.568 J/m^2 per second, UBIO treatment started from the second day after admission, once every other day, with a single course consisting of 5-7 treatments. ② In the UBIO treated group, the venous blood was sampled before and after the first, third and the completion of the treatment course respectively, the venous blood was taken at each corresponding time point in the control group. After centrifugation of the blood at 10 000 rounds per minute, the RBC membrane was separated and then the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase were detected by means of phosphorus determination.③ The nerve function was scored before and after treatment in both groups with European stroke scale, which included 13 items, the total score was 0-100 points, the higher the score, the better the nerve function. MAIN OUTCOME MEASURES ：①Score of European stroke scale before and after treatment in both groups.② Comparison of the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase on RBC membrane between the two groups before treatment and after the first, third and the completion of the treatment. RESULTS： All the 58 patients with cerebral infarction were involved in the analysis of results.① The score of European stroke scale had no obvious difference between the two groups [（49.31±11.48）, （50.58±12.63）, P 〉 0.05], and it was obviously higher in the UBIO treated group than in the control group after treatment [84.66±13.75）, （77.05±11.17）, P 〈 0.05].②The activity of K^＋-Na^＋-ATPase on RBC membrane in the UBIO treated group was significantly increased after the first and third treatment as compared with before treatment [（31.56±19.25）, （27.64±15.83）, （17.67±13.83）, P 〈 0.01], it was still higher after the completion of the treatment than before treatment without obvious difference [（20.86±14.53）, P 〉 0.05]. After the first and third treatment, it was obviously higher in the UBIO treated group than in the control group [19.31±11.88）, （17.44±10.42）, P 〈 0.01]. ③ In the UBIO treated group, Ca2^＋-Mg2^＋-ATPase activity on RBC membrane significantly increased after the first treatment and remained higher than the pre-treatment level throughout the treatment [（27.49±14.72）, （17.41±4.82）, P 〈 0.01]. The activity of Ca2^＋-Mg2^＋-ATPase on RBC membrane was markedly higher in the UBIO treated group than in the control group after after the first, third and the completion of treatment respectively [（24.83±12.88）, （17.70±5.69）; （28.08±13.44）, （16.32±5.29）; （17.42±6.04）, P〈 0.05-0.01]. CONCLUSION： The effect of UBIO treatment against acute cerebral infarction may be mediated by the increased K^＋-Na^＋ ATPase and Ca2^＋-Mg2^＋-ATPase activities on RBC membrane, which enhances the RBC transformation ability so as to lower RBC aggregation and correct high blood viscosity.展开更多
Pre-mRNA splicing is a dynamic process. It is catalyzed by the spliceosome which is a large machine formed by an ordered interactions of several small nuclear ribonucleoproteins, U1,
China's comprehensive national strength has developed rapidly. In the life cycle of modern enterprises, the construction of standardization system is an extremely important work content, which affects the manageme...China's comprehensive national strength has developed rapidly. In the life cycle of modern enterprises, the construction of standardization system is an extremely important work content, which affects the management quality and work order of enterprises to a great extent and plays a decisive role in the healthy development of enterprises. At the same time, the quality of standardization system construction is also a key indicator to measure the core competitiveness and development potential of enterprises. Therefore, it is necessary for us to explore and discuss the related topics of improving the efficiency of enterprise standardization system construction.展开更多
Growth retardation affects the health and production of livestock,while overexertion can cause sudden cardiac arrest.Both cases are considered to be metabolic disorders and are detrimental to livestock production.Effe...Growth retardation affects the health and production of livestock,while overexertion can cause sudden cardiac arrest.Both cases are considered to be metabolic disorders and are detrimental to livestock production.Effective measures for relieving or treating these disorders are scarce.However,Pimpinella thellungiana H.Wolff(P.thellungiana),a medicinal herb,has been reported to relieve growth retardation and overexertion in ethnopharmacological clinical trials.This paper summarizes and classifies a total of 106 bioactive compounds that were isolated and identified from P.thellungiana,including flavonoids,simple phenylpropanoids,coumarins,volatile compounds,and simple polyphenols,and discusses its pharmaceutical benefits,including its growth-promoting,antioxidant,anti-inflammatory,anti-atherosclerotic,and hepatoprotective properties.The nutrition,metabolism,biological activities,and pharmacological effects of the principal compounds of P.thellungiana in livestock are reviewed,as well as their potential molecular targets and metabolic signaling pathways in which these compounds are involved.However,the pharmacological and toxicological effects of some compounds have not been well documented,and further investigations of the bioactive compounds are needed.Such studies are crucial for the development of natural drugs or feed additives from P.thellungiana to alleviate growth retardation and mitigate injuries from overexertion in livestock.展开更多
Grass carp reovirus(GCRV)-induced gene 1(Gig1)is identified initially in crucian carp C.auratus as a novel interferon-stimulated gene(ISG)encoding an antiviral protein and subsequently as the founder member of Gig1 ge...Grass carp reovirus(GCRV)-induced gene 1(Gig1)is identified initially in crucian carp C.auratus as a novel interferon-stimulated gene(ISG)encoding an antiviral protein and subsequently as the founder member of Gig1 gene family.However,the evolution and function of Gig1 family members remain largely unknown.In this study,genome-wide searchs of 100 vertebrate species representing the main taxonomic lineages revealed that the Gig1 family originated from a common ancestor of bony fish but was completely lost in tetrapods.The Gig1 family is divided into three subfamilies,each with its own characteristics in gain and loss.Compared to subfamily Ⅰ that originated the earliest and showed no significant change in gene copies,subfamilies Ⅱ and Ⅲ were expanded robustly with teleost radiation.Despite no known domains having been identified,motif prediction revealed a conserved motif arrangement in all Gig1 family proteins.Functionally,both zebrafish and grass carp have subfamilies Ⅰ and Ⅱ but not subfamily Ⅲ.However,only certain subfamily Ⅱ genes were highly induced by viral infection and thus capable of inhibiting viral replication by overexpression and knockout assays.The Japanese rice fish medaka has no subfamily Ⅱ and,strikingly,one subfamily Ⅲ gene acquired antiviral feature.Site-directed mutation showed that three leucine residues conserved in the motif 1 were essential for the antiviral role of subfamily Ⅱ genes in zebrafish.In summary,our results indicate that bony fish-specific Gig1 gene family has been undergoing diversification in expression and function toward virus infection in a given species.展开更多
The immediate post-weaning period poses a major challenge on the survival of piglets. Similarly, newly hatched chicks face life threatening challenges due to enteric infections. In the past several years, in-feed anti...The immediate post-weaning period poses a major challenge on the survival of piglets. Similarly, newly hatched chicks face life threatening challenges due to enteric infections. In the past several years, in-feed antibiotics have been used to reduce these production problems and improve growth. However, in-feed antibiotics have been banned in many jurisdictions and therefore the most effective alternatives to infeed antibiotics must be developed. To date, several studies have been conducted to develop alternatives to antibiotics. One of the potential candidates as alternatives to in-feed antibiotics is resistant starch(RS). Resistance starch is a type of starch that resists enzymatic digestion in the upper parts of the gastrointestinal tract and therefore passes to hindgut where it can be fermented by resident microorganisms. Microbial fermentation of RS in the hindgut results in the production of short chain fatty acids(SCFA). Production of SCFA in turn results in growth and proliferation of colonic and cecal cells, increased expression of genes involved in gut development, and creation of an acidic environment. The acidic environment suppresses the growth of pathogenic microorganisms while selectively promoting the growth of beneficial microbes. Thus, RS has the potential to improve gut health and function by modifying and stabilising gut microbial community and by improving the immunological status of the host. In this review, we discussed the roles of RS in modifying and stabilising gut microbiota, gut health and function, carcass quality, and energy metabolism and growth performance in pigs and poultry.展开更多
Mountain and polar glaciers cover 10%of the Earth’s surface and are typically extreme environments that challenge life of all forms.Viruses are abundant and active in supraglacial ecosystems and play a crucial role i...Mountain and polar glaciers cover 10%of the Earth’s surface and are typically extreme environments that challenge life of all forms.Viruses are abundant and active in supraglacial ecosystems and play a crucial role in controlling the supraglacial microbial communities.However,our understanding of virus ecology on glacier surfaces and their potential impacts on downstream ecosystems remains limited.Here,we present the supraglacial virus genome(SgVG)catalog,a 15-fold expanded genomic inventory of 10,840 DNAvirus species from 38 mountain and polar glaciers,spanning habitats such as snow,ice,meltwater,and cryoconite.Supraglacial DNA-viruses were highly specific compared to viruses in other ecosystems yet exhibited low public health risks.Supraglacial viral communities were primarily constrained by habitat,with cryoconite displaying the highest viral activity levels.We observed a prevalence of lytic viruses in all habitats,especially in cryoconite,but a high level of lysogenic viruses in snow and ice.Additionally,we found that supraglacial viruses could be linked to~83%of obtained prokaryotic phyla/classes and possessed the genetic potential to promote metabolism and increase cold adaptation,cell mobility,and phenolic carbon use of hosts in hostile environmental conditions using diverse auxiliary metabolic genes.Our results provide the first systematic characterization of the diversity,function,and public health risks evaluation of mountain and polar supraglacial DNA viruses.This understanding of glacial viruses is crucial for function assessments and ecological modeling of glacier ecosystems,especially for the Tibetan Plateau’s Mountain glaciers,which support~20%of the human populations on Earth.展开更多
Dear Editor,Antibody-dependent enhancement（ADE）has long been recognized for dengue virus（DENV）in vitro and in vivo.It is now clear that antibodies to DENV can also enhance Zika virus（ZIKV）infection in vitro,and ...Dear Editor,Antibody-dependent enhancement（ADE）has long been recognized for dengue virus（DENV）in vitro and in vivo.It is now clear that antibodies to DENV can also enhance Zika virus（ZIKV）infection in vitro,and vice versa（Dejnirattisai et al.,2016;Stettler et al.,2016）.The characteristics of enhancing antibodies,however,remain elusive.展开更多
The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rD...The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach,and functional characteristics at community levels by using BIOLOG^(GN) system analysis method as well as two conventional methods(DHA and SIR).All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNA V_(3) high variable region universal primer)indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos,copper,especially some of their combinations had worse effects than their corresponding single factors.In addition,the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community.The effects of the single factors with lower concentrations on the communiy structure were weaker than those with higher concentrations.Moreover,the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors.The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOG^(GN)system were two relatively sensitive directors corresponding to the stress presented in this study.Between methamodophos and copper,there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities.The DHA of soil under the combined stresses was lower than that of the control and that under the single factors,and the BIOLOG^(GN) substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors.From all of above,the methamidophos,copper,especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure,richness,and the functional characteristics of microbial community.展开更多
The effect of parathyroid hormone (PTH) (0.01 nM-10 nM) and 17 -estradiol (E2, 1 nmol-10 nM) alone or in combination on 3H thymidine incorporation, alkaline phosphatase and adenylate cyclase activities were investigat...The effect of parathyroid hormone (PTH) (0.01 nM-10 nM) and 17 -estradiol (E2, 1 nmol-10 nM) alone or in combination on 3H thymidine incorporation, alkaline phosphatase and adenylate cyclase activities were investigated in human fetal osteoblasts using serum-free monolayer primary cultures. The results showed that PTH inhibited cell proliferation while E, promoted it. On alkaline phosphatase activity, PTH showed a complex results while E, were slightly inhibitory. PHT-E2 combination suggested that E2 could alter the effect of PTH alone, also potentiated the anabolic and antagonize the catabolic effects of PTH on bone formation.展开更多
Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism tha...Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism that regulates the germinal center reaction,aphysiological process that underlies high-quality展开更多
1. The focus is on private foundations, which are engaged in advancing science, technology, and the humanities. In this context the Volkswagen Foundationis a prominent example for Germany. 2. Accordingly, the paper do...1. The focus is on private foundations, which are engaged in advancing science, technology, and the humanities. In this context the Volkswagen Foundationis a prominent example for Germany. 2. Accordingly, the paper does not look at the management within firms , but at the systemic level of innovation management. Though innovation has a broadermeaning, including also economic, social, and political aspects. research and higher education are central arenas and factors of change. 3. Contemporary research and higher education are confronted with new challenges, which require new responses and new forms of innovation management: science and technology has become an integral part of the knowledge- based society; the boundaries between basic and applied research are vanishing, problem --oriented research is increasing, as a consequence the demand for inter展开更多
This article analyses different characteristics of scale,site selection and function of the current economic andtehnological development zones in our country.It further proposes that it is inappropriate for the econom...This article analyses different characteristics of scale,site selection and function of the current economic andtehnological development zones in our country.It further proposes that it is inappropriate for the economic andtechnological development zones to follow the“pattern of the special zones”,but a new pattern of“small scale,unitaryfunction and closeness to old city”should be adopted to fit in with the practical conditions in our country.The scale,landuse structure and the relations between the development zone and the old city are the basic issues in planning forthe development zones.These three essential elements constitute the pattern of planning which determines to a certainextent the success or failure of the development zones.展开更多
基金This work was supported by the National Natural Science Foundation of China(31772042)Ramóny Cajal grant(RYC2020-030365-I)+1 种基金Xunta de Galicia for supporting the program(Excelencia-ED431F2022/01)the Key Research&Development Program of Zhejiang Province(2021C02015).
文摘Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly composed of very long-chain fatty acids(VLCFAs);their derivatives,including esters,primary alcohols,secondary alcohols,aldehydes,and ketones;and triterpenoids.This complex mixture of lipids is probably biosynthesized in the epidermal cells of most plants and exuded onto the surface.Cuticular wax not only makes the fruit less susceptible to microbial infection but also reduces mechanical damage to the fruit,thereby maintaining the fruit’s commodity value.To date,research has mostly focused on the changes,function,and regulation of fruit wax before harvest,while ignoring the changes and functions of wax in fruit storage.This paper reviews on the composition,structure,and metabolic regulation of cuticular wax in fruits.It also focuses on postharvest factors affecting wax composition,such as storage temperature,relative humidity(RH),gas atmosphere,and as exogenous hormones;and the effects of wax on fruit postharvest quality,including water dispersion,fruit softening,physiological disorders,and disease resistance.These summaries may be of assistance in better understanding the changes in cuticular wax in postharvest fruit and the resulting effects on fruit quality.
基金the National Natural Science Foundation of China(Nos.41620104001 and 41806131)the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology(No.2016 ASKJ02).
文摘This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and archaeal communities varied greatly with depth.Proteobacteria in bacterial communities and Nitrososphaeria and Woesearchaeota in archaeal communities were dominant in the shallow sediments(1-40 cm),while Chloroflexi in bacterial communities and Bathyarchaeia in archaeal communities were dominant in the deep sediments(50-200 cm).Regarding ecological functions based on the metatranscriptomic data,genes involved in various pathways of nitrogen metabolism and sulfur metabolism were observed in the tested sediment samples.Metagenomic analysis revealed that Proteobacteria contribute the most to nearly all genes involved in nitrogen and sulfur metabolism.Moreover,Thaumarchaeota contribute the most to certain genes involved in nitrification,denitrification and assimilatory sulfate reduction pathways.The most abundant bacterial genus,Candidatus Scalindua,is crucial for nitrification,dissimilatory nitrate reduction,denitrification and assimilatory sulfate reduction pathways.
基金supported by grants from the National Natural Science Foundation of China(91132303,30830035)the National Basic Research Development Program of China (2011CBA00408)the China Postdoctoral Science Foundation(2012M511105)
文摘Ion channels,as membrane proteins,are the sensors of the cell.They act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into unique electrical signals to shape the responses of excitable cells.Because of their importance in cellular communication,ion channels have been intensively studied at the structural and functional levels.Here,we summarize the diverse approaches,including molecular and cellular,chemical,optical,biophysical,and computational,used to probe the structural and functional rearrangements that occur during channel activation（or sensitization）,inactivation（or desensitization）,and various forms of modulation.The emerging insights into the structure and function of ion channels by multidisciplinary approaches allow the development of new pharmacotherapies as well as new tools useful in controlling cellular activity.
文摘As the world’s first country to launch industrial revolution,the UK had once been at the peak of global power for a long term and exerted great inf luence on the shaping of modern
基金Supported by the National Natural Science Foundation of China (31172375)
文摘To investigate the effects of aluminum trichloride （AlCl3） on renal structure and function in chickens, the chickens were injected continuously with different gradient AlCl3 for 60 days to establish sub-chronic aluminum （Al） intoxication model. The AI concentration in serum and kidney, contents of creatinine （Cr） and blood urea nitrogen （BUN） in serum were detected, and the renal structure was also observed by optical microscope. The results showed that the A1 concentration in serum and kidney, the contents of Cr and BUN in serum were significantly higher in Al-treated chickens than those in the control group （P〈0.05; P〈0.01） and there was an AlCl3 dose-depended manner. Histopathologically, in the low dose group, acinus renis swelled mildly and there were no apparent pathological changes in nephric tubule and proximal convoluted tubule. In the middle and high dose group, acinus renis swelled, glomerular cells increased, nephric tubule cloudy swelled, and epithelial cells of proximal convoluted tubule swelled. The results indicated that sub-chronic Al exposure impaired the renal structure and function in chickens.
文摘BACKGROUND： Ultraviolet blood irradiation and oxygenation （UBIO） has obtained better clinical effect in treating acute cerebral infarction, but the mechanism underlying this effect remains unclear. OBJECTIVE： To observe the effect of UBIO on the nerve function and activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase activities on the red blood cell （RBC） membrane of patients with acute cerebral infarction. DESIGN： A randomized and controlled study.SETTING： Department of Neurology, Xiangfan Central Hospital.PARTICIPANTS： From January 2000 to December 2001, excluding those above 70 years old, 58 cases of 700 patients with acute cerebral infarction admitted in the Department of Neurology, Xiangfan Central Hospital, were recruited and divided into two groups according to the random number table： UBIO treated group （n=28）, including 17 males and 11 females, aged 40-68 years; and control group （n=30）, including 20 males and 10 females, aged 44-69 years. All the patients agreed to participate in the therapeutic program and detected items. The general informations were comparable without obvious differences between the two groups （P 〉 0.05）.METHODS： ① The patients in both groups received routine treatments, besides, those in the UBIO treated group were given UBIO treatment by using the XL-200 type therapeutic apparatus produced in Shijiazhuang, whose ultraviolet wave was set at 253.7 nm with the energy density of 0.568 J/m^2 per second, UBIO treatment started from the second day after admission, once every other day, with a single course consisting of 5-7 treatments. ② In the UBIO treated group, the venous blood was sampled before and after the first, third and the completion of the treatment course respectively, the venous blood was taken at each corresponding time point in the control group. After centrifugation of the blood at 10 000 rounds per minute, the RBC membrane was separated and then the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase were detected by means of phosphorus determination.③ The nerve function was scored before and after treatment in both groups with European stroke scale, which included 13 items, the total score was 0-100 points, the higher the score, the better the nerve function. MAIN OUTCOME MEASURES ：①Score of European stroke scale before and after treatment in both groups.② Comparison of the activities of K^＋-Na^＋-ATPase and Ca2^＋-Mg2^＋-ATPase on RBC membrane between the two groups before treatment and after the first, third and the completion of the treatment. RESULTS： All the 58 patients with cerebral infarction were involved in the analysis of results.① The score of European stroke scale had no obvious difference between the two groups [（49.31±11.48）, （50.58±12.63）, P 〉 0.05], and it was obviously higher in the UBIO treated group than in the control group after treatment [84.66±13.75）, （77.05±11.17）, P 〈 0.05].②The activity of K^＋-Na^＋-ATPase on RBC membrane in the UBIO treated group was significantly increased after the first and third treatment as compared with before treatment [（31.56±19.25）, （27.64±15.83）, （17.67±13.83）, P 〈 0.01], it was still higher after the completion of the treatment than before treatment without obvious difference [（20.86±14.53）, P 〉 0.05]. After the first and third treatment, it was obviously higher in the UBIO treated group than in the control group [19.31±11.88）, （17.44±10.42）, P 〈 0.01]. ③ In the UBIO treated group, Ca2^＋-Mg2^＋-ATPase activity on RBC membrane significantly increased after the first treatment and remained higher than the pre-treatment level throughout the treatment [（27.49±14.72）, （17.41±4.82）, P 〈 0.01]. The activity of Ca2^＋-Mg2^＋-ATPase on RBC membrane was markedly higher in the UBIO treated group than in the control group after after the first, third and the completion of treatment respectively [（24.83±12.88）, （17.70±5.69）; （28.08±13.44）, （16.32±5.29）; （17.42±6.04）, P〈 0.05-0.01]. CONCLUSION： The effect of UBIO treatment against acute cerebral infarction may be mediated by the increased K^＋-Na^＋ ATPase and Ca2^＋-Mg2^＋-ATPase activities on RBC membrane, which enhances the RBC transformation ability so as to lower RBC aggregation and correct high blood viscosity.
文摘Pre-mRNA splicing is a dynamic process. It is catalyzed by the spliceosome which is a large machine formed by an ordered interactions of several small nuclear ribonucleoproteins, U1,
文摘China's comprehensive national strength has developed rapidly. In the life cycle of modern enterprises, the construction of standardization system is an extremely important work content, which affects the management quality and work order of enterprises to a great extent and plays a decisive role in the healthy development of enterprises. At the same time, the quality of standardization system construction is also a key indicator to measure the core competitiveness and development potential of enterprises. Therefore, it is necessary for us to explore and discuss the related topics of improving the efficiency of enterprise standardization system construction.
基金supported by the National Natural Science Foundation of China[32260844]Tianshan Talent Project of Xinjiang[2024]+1 种基金Bingtuan Science and Technology Program[2023AB078]International Partnership Program of the Chinese Academy of Sciences[092GJHZ2023062GC].
文摘Growth retardation affects the health and production of livestock,while overexertion can cause sudden cardiac arrest.Both cases are considered to be metabolic disorders and are detrimental to livestock production.Effective measures for relieving or treating these disorders are scarce.However,Pimpinella thellungiana H.Wolff(P.thellungiana),a medicinal herb,has been reported to relieve growth retardation and overexertion in ethnopharmacological clinical trials.This paper summarizes and classifies a total of 106 bioactive compounds that were isolated and identified from P.thellungiana,including flavonoids,simple phenylpropanoids,coumarins,volatile compounds,and simple polyphenols,and discusses its pharmaceutical benefits,including its growth-promoting,antioxidant,anti-inflammatory,anti-atherosclerotic,and hepatoprotective properties.The nutrition,metabolism,biological activities,and pharmacological effects of the principal compounds of P.thellungiana in livestock are reviewed,as well as their potential molecular targets and metabolic signaling pathways in which these compounds are involved.However,the pharmacological and toxicological effects of some compounds have not been well documented,and further investigations of the bioactive compounds are needed.Such studies are crucial for the development of natural drugs or feed additives from P.thellungiana to alleviate growth retardation and mitigate injuries from overexertion in livestock.
基金supported by grants from the National Key R&D Program of China(2022YFF1000302)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0730100)+1 种基金the National Natural Science Foundation of China(32473197,32403073,32202978)the Ministry of Science and Technology of China(grant number:2023ZD04065).
文摘Grass carp reovirus(GCRV)-induced gene 1(Gig1)is identified initially in crucian carp C.auratus as a novel interferon-stimulated gene(ISG)encoding an antiviral protein and subsequently as the founder member of Gig1 gene family.However,the evolution and function of Gig1 family members remain largely unknown.In this study,genome-wide searchs of 100 vertebrate species representing the main taxonomic lineages revealed that the Gig1 family originated from a common ancestor of bony fish but was completely lost in tetrapods.The Gig1 family is divided into three subfamilies,each with its own characteristics in gain and loss.Compared to subfamily Ⅰ that originated the earliest and showed no significant change in gene copies,subfamilies Ⅱ and Ⅲ were expanded robustly with teleost radiation.Despite no known domains having been identified,motif prediction revealed a conserved motif arrangement in all Gig1 family proteins.Functionally,both zebrafish and grass carp have subfamilies Ⅰ and Ⅱ but not subfamily Ⅲ.However,only certain subfamily Ⅱ genes were highly induced by viral infection and thus capable of inhibiting viral replication by overexpression and knockout assays.The Japanese rice fish medaka has no subfamily Ⅱ and,strikingly,one subfamily Ⅲ gene acquired antiviral feature.Site-directed mutation showed that three leucine residues conserved in the motif 1 were essential for the antiviral role of subfamily Ⅱ genes in zebrafish.In summary,our results indicate that bony fish-specific Gig1 gene family has been undergoing diversification in expression and function toward virus infection in a given species.
文摘The immediate post-weaning period poses a major challenge on the survival of piglets. Similarly, newly hatched chicks face life threatening challenges due to enteric infections. In the past several years, in-feed antibiotics have been used to reduce these production problems and improve growth. However, in-feed antibiotics have been banned in many jurisdictions and therefore the most effective alternatives to infeed antibiotics must be developed. To date, several studies have been conducted to develop alternatives to antibiotics. One of the potential candidates as alternatives to in-feed antibiotics is resistant starch(RS). Resistance starch is a type of starch that resists enzymatic digestion in the upper parts of the gastrointestinal tract and therefore passes to hindgut where it can be fermented by resident microorganisms. Microbial fermentation of RS in the hindgut results in the production of short chain fatty acids(SCFA). Production of SCFA in turn results in growth and proliferation of colonic and cecal cells, increased expression of genes involved in gut development, and creation of an acidic environment. The acidic environment suppresses the growth of pathogenic microorganisms while selectively promoting the growth of beneficial microbes. Thus, RS has the potential to improve gut health and function by modifying and stabilising gut microbial community and by improving the immunological status of the host. In this review, we discussed the roles of RS in modifying and stabilising gut microbiota, gut health and function, carcass quality, and energy metabolism and growth performance in pigs and poultry.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2021QZKK0100)the National Key Research and Development Plans(2021YFC0863400)+2 种基金the National Key Research and Development Plans(2021YFC2300904)the National Natural Science Foundation of China General Program(42171144)the National Natural Science Foundation of China for Excellent Young Scientists Fund Program(42222105)。
文摘Mountain and polar glaciers cover 10%of the Earth’s surface and are typically extreme environments that challenge life of all forms.Viruses are abundant and active in supraglacial ecosystems and play a crucial role in controlling the supraglacial microbial communities.However,our understanding of virus ecology on glacier surfaces and their potential impacts on downstream ecosystems remains limited.Here,we present the supraglacial virus genome(SgVG)catalog,a 15-fold expanded genomic inventory of 10,840 DNAvirus species from 38 mountain and polar glaciers,spanning habitats such as snow,ice,meltwater,and cryoconite.Supraglacial DNA-viruses were highly specific compared to viruses in other ecosystems yet exhibited low public health risks.Supraglacial viral communities were primarily constrained by habitat,with cryoconite displaying the highest viral activity levels.We observed a prevalence of lytic viruses in all habitats,especially in cryoconite,but a high level of lysogenic viruses in snow and ice.Additionally,we found that supraglacial viruses could be linked to~83%of obtained prokaryotic phyla/classes and possessed the genetic potential to promote metabolism and increase cold adaptation,cell mobility,and phenolic carbon use of hosts in hostile environmental conditions using diverse auxiliary metabolic genes.Our results provide the first systematic characterization of the diversity,function,and public health risks evaluation of mountain and polar supraglacial DNA viruses.This understanding of glacial viruses is crucial for function assessments and ecological modeling of glacier ecosystems,especially for the Tibetan Plateau’s Mountain glaciers,which support~20%of the human populations on Earth.
基金supported by National Key Program Project Grant of Ministry of Science and Technology of China(2016YFC1201000)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDBP 030405)grants from the Municipal Science and Technology Bureau Foundation of Guangzhou(2014Y2-00550,201508020263)
文摘Dear Editor,Antibody-dependent enhancement（ADE）has long been recognized for dengue virus（DENV）in vitro and in vivo.It is now clear that antibodies to DENV can also enhance Zika virus（ZIKV）infection in vitro,and vice versa（Dejnirattisai et al.,2016;Stettler et al.,2016）.The characteristics of enhancing antibodies,however,remain elusive.
基金This work was supported in part by the Knowledge Innovation Engineering Action,the Chinese Academy of Sciences(KZCX2-SW-416)the National Natural Science Foundation of China(Grant No.20225722).
文摘The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach,and functional characteristics at community levels by using BIOLOG^(GN) system analysis method as well as two conventional methods(DHA and SIR).All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNA V_(3) high variable region universal primer)indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos,copper,especially some of their combinations had worse effects than their corresponding single factors.In addition,the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community.The effects of the single factors with lower concentrations on the communiy structure were weaker than those with higher concentrations.Moreover,the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors.The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOG^(GN)system were two relatively sensitive directors corresponding to the stress presented in this study.Between methamodophos and copper,there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities.The DHA of soil under the combined stresses was lower than that of the control and that under the single factors,and the BIOLOG^(GN) substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors.From all of above,the methamidophos,copper,especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure,richness,and the functional characteristics of microbial community.
文摘The effect of parathyroid hormone (PTH) (0.01 nM-10 nM) and 17 -estradiol (E2, 1 nmol-10 nM) alone or in combination on 3H thymidine incorporation, alkaline phosphatase and adenylate cyclase activities were investigated in human fetal osteoblasts using serum-free monolayer primary cultures. The results showed that PTH inhibited cell proliferation while E, promoted it. On alkaline phosphatase activity, PTH showed a complex results while E, were slightly inhibitory. PHT-E2 combination suggested that E2 could alter the effect of PTH alone, also potentiated the anabolic and antagonize the catabolic effects of PTH on bone formation.
文摘Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism that regulates the germinal center reaction,aphysiological process that underlies high-quality
文摘1. The focus is on private foundations, which are engaged in advancing science, technology, and the humanities. In this context the Volkswagen Foundationis a prominent example for Germany. 2. Accordingly, the paper does not look at the management within firms , but at the systemic level of innovation management. Though innovation has a broadermeaning, including also economic, social, and political aspects. research and higher education are central arenas and factors of change. 3. Contemporary research and higher education are confronted with new challenges, which require new responses and new forms of innovation management: science and technology has become an integral part of the knowledge- based society; the boundaries between basic and applied research are vanishing, problem --oriented research is increasing, as a consequence the demand for inter
文摘This article analyses different characteristics of scale,site selection and function of the current economic andtehnological development zones in our country.It further proposes that it is inappropriate for the economic andtechnological development zones to follow the“pattern of the special zones”,but a new pattern of“small scale,unitaryfunction and closeness to old city”should be adopted to fit in with the practical conditions in our country.The scale,landuse structure and the relations between the development zone and the old city are the basic issues in planning forthe development zones.These three essential elements constitute the pattern of planning which determines to a certainextent the success or failure of the development zones.
