Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT...Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT116 cells were the control group.The mRNA expression of the two groupsswas detected by transcriptome sequencing.The differentially expressed genes were screened,and the functions and signaling pathways which differentially expressed genes were involved in were preliminarily analyzed through Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KECG)pathway enrichment analysis,and effect of DHHC7 on the invasive capacity of tumor cells was verified by Transwell invasion assay.Results There were 2099 differentially expressed genes between the knockout group and the control group,of which 1190 were downregulated and 909 were upregulated.Significantly enriched GO items included cell adhesion,cell membrane composition,etc.The results of KECC pathway enrichment analysis suggested that DHHC7 might be involved in extracelluar matrix-receptor interaction and cell adhesion molecular pathway.The result of transwell assay confirmed that ZDHHC7 knockout could inhibit the invasion of tumor cells.Conclusion DHHC7 may promote the invasiveness of colorectal cancer by regulating the destruction of extracellular matrix and the expression of cell adhesion molecules.展开更多
目的：观察国产紫杉醇联合顺铂治疗Ⅲ～Ⅳ期非小细胞肺癌（non-small cell lung cancer，NSCLC）的近期疗效、毒副反应及恶性肿瘤相关物质（tumor supplied group of factors，TSGF）的变化。方法：初治的Ⅲ～Ⅳ期NSCLC患者40例，以国...目的：观察国产紫杉醇联合顺铂治疗Ⅲ～Ⅳ期非小细胞肺癌（non-small cell lung cancer，NSCLC）的近期疗效、毒副反应及恶性肿瘤相关物质（tumor supplied group of factors，TSGF）的变化。方法：初治的Ⅲ～Ⅳ期NSCLC患者40例，以国产紫杉醇135ms／m^2，静脉滴注，d1，顺铂30ms／m^2，静脉滴注，d1～d3，连用2个周期后评价疗效，TSGF应用生化比色法检测。结果：全组35例可评价，部分缓解（PR）15例，稳定（SD）12例，进展（PD）8例，有效率（CR＋PR）为42．9％。主要毒副反应为胃肠反应（Ⅲ～Ⅳ级为11．4％）和血液学毒性（Ⅲ-Ⅳ级白细胞下降为14．2％）。在治疗有效的患者中治疗后TSGF明显下降（P〈0．001），而在治疗无效的患者中治疗后TSGF无明显下降（P〉0．05）。结论：国产紫杉醇联合顺铂治疗NSCLC有较好疗效，毒性较小，TSGF可作为肺癌治疗疗效的评价依据之一。展开更多
文摘Objective To explore the role and mechanism of palmitoyltransferase DHHC7 in colorectal cancer invasiveness.Methods Colorectacl ancer cell lines HCT116 with ZDHHC7 knocked out were the knockout group,and wild-type HCT116 cells were the control group.The mRNA expression of the two groupsswas detected by transcriptome sequencing.The differentially expressed genes were screened,and the functions and signaling pathways which differentially expressed genes were involved in were preliminarily analyzed through Gene Ontology(GO)function analysis and Kyoto Encyclopedia of Genes and Genomes(KECG)pathway enrichment analysis,and effect of DHHC7 on the invasive capacity of tumor cells was verified by Transwell invasion assay.Results There were 2099 differentially expressed genes between the knockout group and the control group,of which 1190 were downregulated and 909 were upregulated.Significantly enriched GO items included cell adhesion,cell membrane composition,etc.The results of KECC pathway enrichment analysis suggested that DHHC7 might be involved in extracelluar matrix-receptor interaction and cell adhesion molecular pathway.The result of transwell assay confirmed that ZDHHC7 knockout could inhibit the invasion of tumor cells.Conclusion DHHC7 may promote the invasiveness of colorectal cancer by regulating the destruction of extracellular matrix and the expression of cell adhesion molecules.
文摘目的：观察国产紫杉醇联合顺铂治疗Ⅲ～Ⅳ期非小细胞肺癌（non-small cell lung cancer，NSCLC）的近期疗效、毒副反应及恶性肿瘤相关物质（tumor supplied group of factors，TSGF）的变化。方法：初治的Ⅲ～Ⅳ期NSCLC患者40例，以国产紫杉醇135ms／m^2，静脉滴注，d1，顺铂30ms／m^2，静脉滴注，d1～d3，连用2个周期后评价疗效，TSGF应用生化比色法检测。结果：全组35例可评价，部分缓解（PR）15例，稳定（SD）12例，进展（PD）8例，有效率（CR＋PR）为42．9％。主要毒副反应为胃肠反应（Ⅲ～Ⅳ级为11．4％）和血液学毒性（Ⅲ-Ⅳ级白细胞下降为14．2％）。在治疗有效的患者中治疗后TSGF明显下降（P〈0．001），而在治疗无效的患者中治疗后TSGF无明显下降（P〉0．05）。结论：国产紫杉醇联合顺铂治疗NSCLC有较好疗效，毒性较小，TSGF可作为肺癌治疗疗效的评价依据之一。
