Metagenomic next-generation sequencing(mNGS)is a culture-independent technique that directly extracts and sequences all nucleic acids from clinical specimens.By leveraging high-throughput sequencing and bioinformatic ...Metagenomic next-generation sequencing(mNGS)is a culture-independent technique that directly extracts and sequences all nucleic acids from clinical specimens.By leveraging high-throughput sequencing and bioinformatic analysis,it characterizes the entire microbial landscape,including bacteria,fungi,viruses,and parasites.This approach significantly broadens detection coverage,improves sensitivity,and reduces turnaround time compared with conventional diagnostic methods.Since its initial application to suspected infectious uveitis by Doan et al.in 2006,mNGS has been increasingly integrated into ophthalmic practice to facilitate pathogen identification or exclusion[1].展开更多
This paper reviews the history and lessons of global oil crises while exploring the establishment of a quantitative evaluation model for oil security with Chinese characteristics.Using principal component analysis,it ...This paper reviews the history and lessons of global oil crises while exploring the establishment of a quantitative evaluation model for oil security with Chinese characteristics.Using principal component analysis,it constructs an oil security evaluation indicator system for China with two main-level indicators:foreign oil dependency and its impacts,and market intervention and security assurance.展开更多
Impact statementThis study identifies avian tuberculosis as a potential cause of mass mortality in wild migratory birds in Inner Mongolia,China.Combining meta-transcriptomic sequencing and histopathological analysis,it...Impact statementThis study identifies avian tuberculosis as a potential cause of mass mortality in wild migratory birds in Inner Mongolia,China.Combining meta-transcriptomic sequencing and histopathological analysis,it reveals one of the rare instances oftuberculosis-associated outbreaks in avian populations.Thesefindings underscore the importance of surveillance on wildlifediseases to mitigate the risk of interspecies transmission of the disease associated pathogens and their broader implicationsfor biodiversity and public health.展开更多
基金supported by the Beijing Hospitals Authority’s Ascent Programme(DFL20220301)Beijing Nova Program(20230484445)+2 种基金Capital Health Development Scientific Research Project Grant(SF 2022-2-2035 to Y.T.)Excellent Young Talent Innovation Project of Chinese Institutes for Medical Research(CX23YQA02)the Chinese Institute for Medical Research(CX23YQ03).
文摘Metagenomic next-generation sequencing(mNGS)is a culture-independent technique that directly extracts and sequences all nucleic acids from clinical specimens.By leveraging high-throughput sequencing and bioinformatic analysis,it characterizes the entire microbial landscape,including bacteria,fungi,viruses,and parasites.This approach significantly broadens detection coverage,improves sensitivity,and reduces turnaround time compared with conventional diagnostic methods.Since its initial application to suspected infectious uveitis by Doan et al.in 2006,mNGS has been increasingly integrated into ophthalmic practice to facilitate pathogen identification or exclusion[1].
文摘This paper reviews the history and lessons of global oil crises while exploring the establishment of a quantitative evaluation model for oil security with Chinese characteristics.Using principal component analysis,it constructs an oil security evaluation indicator system for China with two main-level indicators:foreign oil dependency and its impacts,and market intervention and security assurance.
基金approved by the Animal Ethics Committee ofthe Institute of Microbiology,Chinese Academy of Sciences(Approval Number:APIMCAS2018034 and APIMCAS2021112).
文摘Impact statementThis study identifies avian tuberculosis as a potential cause of mass mortality in wild migratory birds in Inner Mongolia,China.Combining meta-transcriptomic sequencing and histopathological analysis,it reveals one of the rare instances oftuberculosis-associated outbreaks in avian populations.Thesefindings underscore the importance of surveillance on wildlifediseases to mitigate the risk of interspecies transmission of the disease associated pathogens and their broader implicationsfor biodiversity and public health.
