Devices supporting work in multi-physical environments present new challenges for material design.Due to the wavelength difference,waves from multi-field are difficult to modulate simultaneously,limiting the multi-fie...Devices supporting work in multi-physical environments present new challenges for material design.Due to the wavelength difference,waves from multi-field are difficult to modulate simultaneously,limiting the multi-field functions integration.Inspired by characteristic scale analysis,in this work,a devisable metasurface with characteristic scale compatibility is proposed.Under the reduced characteristic scale,waves in microwave,infrared,and acoustic fields can be modulated simultaneously,which can realize the multi-physics functions compatibility.In the microwave field,the far-field performance can be modulated by designing wavefront phase distribution.In the infrared field,the infrared radiation characteristic can be spatially modulated through noninvasive insetting of infrared devices in the microwave layer.In the acoustic field,the sound wave entering the metasurface can realize high-efficiency loss under the action of the Helmholtz cavity.To verify the design method,a functional sample is simulated and experimented.Three typical functions are effectively verified,which can realize 10 dB backward scattering reduction at 8-10 GHz,digital infrared camouflage with infrared emissivity modulation from 0.4 to 0.8 at 3-14μm,and sound absorptivity of more than 60%at 160-410 Hz,respectively.The comparable characteristic scale design method paves a new way for individually devisable metasurfaces in multi-physical field integration.展开更多
Objective To investigate the mechanism of benzyl isothiocyanate(BITC)in the treatment of anaplastic thyroid cancer(ATC).Methods Using network pharmacological analysis,key targets of BITC and ATC were screened,followed...Objective To investigate the mechanism of benzyl isothiocyanate(BITC)in the treatment of anaplastic thyroid cancer(ATC).Methods Using network pharmacological analysis,key targets of BITC and ATC were screened,followed by CO and KEGG enrichment analysis.In order to validate the findings,AutoDock software was used to dock BITC and ATC key targets.BITC was applied to two ATC cell lines(8505C and CAL-62).Flow cytometry was used to analyze cell apoptosis.Autophagy inhibitors hydroxychloroquine sulfate(HCQ)and 3-methyladenine(3MA)were used in combination with BITC.Real-time quantitative PCR was conducted to detect the gene level of LC3B,while Western blotting was utilized to examine the expression of NF-kB,LC3B I,Beclin-1,and Bcl-2.In animal experiments,a mouse tumor model was constructed using CAL-62 cells,treated with intraperitoneal injections of BITC(100 mg/kg)and normal saline respectively,administered every other day for a total of 21 days.Immunoblotting of tumor tissue was performed to detect the expression of LC3B II,Bcl-2,Beclin-1,and NFkB.Results A total of 10 key targets with binding energies≤-4.0 kcal/mol were identified.KECG analysis showed that these genes are mainly involved in NF-kB signaling pathway and apoptosis.BITC inhibited ATC cells with IC50 values of 27.56μmol/L for 8505C and 28.30μmol/L for CAL-62.The expression levels of NF-kB,Beclin-1,and Bcl-2 decreased,while LC3B I and LC3B gene expression increased.Combining 3MA with BITC enhanced cell inhibition LC3B II expression.HCQ increased LC3B II expression without enhancing cell and viability inhibition.In the mouse tumor model,compared to the control group,the treatment group had higher LC3B II and lower Bcl-2,Beclin-1,and NF-kB levels.Conclusion BITC could inhibit the growth of ATC cells in vitro and in vivo,disrupt the autophagy degradation,and inhibit the NF-kB pathway.展开更多
基金Natural Science Foundation for Young Scientists of Shaanxi Province(2024JC-YBMS-504)Ministry of Science and Technology of the People's Republic of China(2022YFB3806200)+1 种基金Shaanxi Key Science and Technology Innovation Team Project(2023-CX-TD-48)National Natural Science Foundation of China(62401614)。
文摘Devices supporting work in multi-physical environments present new challenges for material design.Due to the wavelength difference,waves from multi-field are difficult to modulate simultaneously,limiting the multi-field functions integration.Inspired by characteristic scale analysis,in this work,a devisable metasurface with characteristic scale compatibility is proposed.Under the reduced characteristic scale,waves in microwave,infrared,and acoustic fields can be modulated simultaneously,which can realize the multi-physics functions compatibility.In the microwave field,the far-field performance can be modulated by designing wavefront phase distribution.In the infrared field,the infrared radiation characteristic can be spatially modulated through noninvasive insetting of infrared devices in the microwave layer.In the acoustic field,the sound wave entering the metasurface can realize high-efficiency loss under the action of the Helmholtz cavity.To verify the design method,a functional sample is simulated and experimented.Three typical functions are effectively verified,which can realize 10 dB backward scattering reduction at 8-10 GHz,digital infrared camouflage with infrared emissivity modulation from 0.4 to 0.8 at 3-14μm,and sound absorptivity of more than 60%at 160-410 Hz,respectively.The comparable characteristic scale design method paves a new way for individually devisable metasurfaces in multi-physical field integration.
文摘Objective To investigate the mechanism of benzyl isothiocyanate(BITC)in the treatment of anaplastic thyroid cancer(ATC).Methods Using network pharmacological analysis,key targets of BITC and ATC were screened,followed by CO and KEGG enrichment analysis.In order to validate the findings,AutoDock software was used to dock BITC and ATC key targets.BITC was applied to two ATC cell lines(8505C and CAL-62).Flow cytometry was used to analyze cell apoptosis.Autophagy inhibitors hydroxychloroquine sulfate(HCQ)and 3-methyladenine(3MA)were used in combination with BITC.Real-time quantitative PCR was conducted to detect the gene level of LC3B,while Western blotting was utilized to examine the expression of NF-kB,LC3B I,Beclin-1,and Bcl-2.In animal experiments,a mouse tumor model was constructed using CAL-62 cells,treated with intraperitoneal injections of BITC(100 mg/kg)and normal saline respectively,administered every other day for a total of 21 days.Immunoblotting of tumor tissue was performed to detect the expression of LC3B II,Bcl-2,Beclin-1,and NFkB.Results A total of 10 key targets with binding energies≤-4.0 kcal/mol were identified.KECG analysis showed that these genes are mainly involved in NF-kB signaling pathway and apoptosis.BITC inhibited ATC cells with IC50 values of 27.56μmol/L for 8505C and 28.30μmol/L for CAL-62.The expression levels of NF-kB,Beclin-1,and Bcl-2 decreased,while LC3B I and LC3B gene expression increased.Combining 3MA with BITC enhanced cell inhibition LC3B II expression.HCQ increased LC3B II expression without enhancing cell and viability inhibition.In the mouse tumor model,compared to the control group,the treatment group had higher LC3B II and lower Bcl-2,Beclin-1,and NF-kB levels.Conclusion BITC could inhibit the growth of ATC cells in vitro and in vivo,disrupt the autophagy degradation,and inhibit the NF-kB pathway.
