Vibrational imaging,comprised of Raman scattering and infrared(IR)absorptionmicroscopy,is undergoing a transformative evolution,driven by codevelopments in physical principles,instrumentation,vibrational probes,sample...Vibrational imaging,comprised of Raman scattering and infrared(IR)absorptionmicroscopy,is undergoing a transformative evolution,driven by codevelopments in physical principles,instrumentation,vibrational probes,sample prep-aration,and computational analysis.By directly visualizing chemical bonds in situ,bond-selectiveimaging reveals biochemical complexity that is often inaccessible to traditional fluorescence-based approaches.A recent Nature Methods focus issue highlighted the conceptual and technological advances that have propelled bond-selective imaging from its roots in physical chemistry into the forefront of life science and biomedicine.Vibrational imaging techniques leverage the intrinsic vibrational contrast of chemical bonds to probe biological systemswith molecular specifcity.展开更多
基金the National Natural Science Foundation of China(Grant No.22377016)the National Key R&D Program of China(Grant No.2024YFC3406500)+1 种基金the Fund of Fudan University and Cao’ejiang Basic Research(Grant No.24FCA08)start-up funding from Fudan University and Shanghai Xuhui Central Hospital.
文摘Vibrational imaging,comprised of Raman scattering and infrared(IR)absorptionmicroscopy,is undergoing a transformative evolution,driven by codevelopments in physical principles,instrumentation,vibrational probes,sample prep-aration,and computational analysis.By directly visualizing chemical bonds in situ,bond-selectiveimaging reveals biochemical complexity that is often inaccessible to traditional fluorescence-based approaches.A recent Nature Methods focus issue highlighted the conceptual and technological advances that have propelled bond-selective imaging from its roots in physical chemistry into the forefront of life science and biomedicine.Vibrational imaging techniques leverage the intrinsic vibrational contrast of chemical bonds to probe biological systemswith molecular specifcity.
