Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro...Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.展开更多
Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'...Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.展开更多
Urban air quality degradation from rising CO_(2) is acute in rapidly developing tropical cities such as Makassar,Indonesia.We deploy a drone-based Internet of Things(IoT)platform for real-time CO_(2) monitoring,integr...Urban air quality degradation from rising CO_(2) is acute in rapidly developing tropical cities such as Makassar,Indonesia.We deploy a drone-based Internet of Things(IoT)platform for real-time CO_(2) monitoring,integrating low-cost sensors(NDIR,MQ135,MG811)on a DJI Phantom 4 with cloud streaming to Firebase.Measurements were collected at five sites,namely Jl.AP.Pettarani,Jl.Ahmad Yani,Jl.Sultan Hasanuddin,Jl.Nusantara,and KIMA at 08:00,12:00,and 16:00 in September 2024 while vertically profiling 1-20 m with three repeat flights per site and time.Descriptive statistics and one-way ANOVA with Tukey HSD assessed spatio-temporal differences;Pearson correlation quantified cross-sensor agreement.Results show marked spatial and diurnal variability:Jl.AP.Pettarani exhibits the highest mean concentration(442.5 ppm),likely due to flyover-induced trapping,whereas Jl.Ahmad Yani records the lowest(390.0 ppm).Vertical profiles reveal mid-altitude peaks in street-canyon and industrial settings,and dilution with height in greener areas,indicating ventilation contrasts.Preprocessing removed outliers and applied temperature-humidity corrections to low-cost sensors.Differences across locations and times are statistically significant(p<0.05),and cross-sensor correlations are strong(r≈0.88-0.96)after correction.Compared with fixed ground stations,the system provides fine-scale three-dimensional coverage and real-time visualization useful for field decisions.Limitations include payload-constrained endurance and intermittent data loss in obstructed areas.Findings support targeted interventions,improving canyon ventilation around flyovers and expanding urban greenery relevant to Makassar and similar tropical cities.展开更多
基金supported by Qingdao Key Medical and Health Discipline ProjectThe Intramural Research Program of the Affiliated Hospital of Qingdao University,No. 4910Qingdao West Coast New Area Science and Technology Project,No. 2020-55 (all to SW)。
文摘Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.
文摘Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.
基金supported by the Directorate of Research,Technology,and Community Service(DRTPM),Ministry of Education,Culture,Research,and Technology,grant number 2817/UN36.11/LP2M/2024.
文摘Urban air quality degradation from rising CO_(2) is acute in rapidly developing tropical cities such as Makassar,Indonesia.We deploy a drone-based Internet of Things(IoT)platform for real-time CO_(2) monitoring,integrating low-cost sensors(NDIR,MQ135,MG811)on a DJI Phantom 4 with cloud streaming to Firebase.Measurements were collected at five sites,namely Jl.AP.Pettarani,Jl.Ahmad Yani,Jl.Sultan Hasanuddin,Jl.Nusantara,and KIMA at 08:00,12:00,and 16:00 in September 2024 while vertically profiling 1-20 m with three repeat flights per site and time.Descriptive statistics and one-way ANOVA with Tukey HSD assessed spatio-temporal differences;Pearson correlation quantified cross-sensor agreement.Results show marked spatial and diurnal variability:Jl.AP.Pettarani exhibits the highest mean concentration(442.5 ppm),likely due to flyover-induced trapping,whereas Jl.Ahmad Yani records the lowest(390.0 ppm).Vertical profiles reveal mid-altitude peaks in street-canyon and industrial settings,and dilution with height in greener areas,indicating ventilation contrasts.Preprocessing removed outliers and applied temperature-humidity corrections to low-cost sensors.Differences across locations and times are statistically significant(p<0.05),and cross-sensor correlations are strong(r≈0.88-0.96)after correction.Compared with fixed ground stations,the system provides fine-scale three-dimensional coverage and real-time visualization useful for field decisions.Limitations include payload-constrained endurance and intermittent data loss in obstructed areas.Findings support targeted interventions,improving canyon ventilation around flyovers and expanding urban greenery relevant to Makassar and similar tropical cities.
