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Fractal Method for Statistical Analysis Geological Data 被引量:2
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作者 Meng Xianguo Zhao PengdaChina University of Geosciences , Wuhan 430074 《Journal of Earth Science》 SCIE CAS CSCD 1991年第1期114-119,共6页
This paper establishes the phase space in the light of spacial series data , discusses the fractal structure of geological data in terms of correlated functions and studies the chaos of these data . In addition , it i... This paper establishes the phase space in the light of spacial series data , discusses the fractal structure of geological data in terms of correlated functions and studies the chaos of these data . In addition , it introduces the R/S analysis for time series analysis into spacial series to calculate the structural fractal dimensions of ranges and standard deviation for spacial series data -and to establish the fractal dimension matrix and the procedures in plotting the fractal dimension anomaly diagram with vector distances of fractal dimension . At last , it has examples of its application . 展开更多
关键词 geological data fractal method fractal dimension space series R/S analysis .
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3D slope stability analysis considering strength anisotropy by a microstructure tensor enhanced elasto-plastic finite element method 被引量:1
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作者 Wencheng Wei Hongxiang Tang +1 位作者 Xiaoyu Song Xiangji Ye 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1664-1684,共21页
This article presents a micro-structure tensor enhanced elasto-plastic finite element(FE)method to address strength anisotropy in three-dimensional(3D)soil slope stability analysis.The gravity increase method(GIM)is e... This article presents a micro-structure tensor enhanced elasto-plastic finite element(FE)method to address strength anisotropy in three-dimensional(3D)soil slope stability analysis.The gravity increase method(GIM)is employed to analyze the stability of 3D anisotropic soil slopes.The accuracy of the proposed method is first verified against the data in the literature.We then simulate the 3D soil slope with a straight slope surface and the convex and concave slope surfaces with a 90turning corner to study the 3D effect on slope stability and the failure mechanism under anisotropy conditions.Based on our numerical results,the end effect significantly impacts the failure mechanism and safety factor.Anisotropy degree notably affects the safety factor,with higher degrees leading to deeper landslides.For concave slopes,they can be approximated by straight slopes with suitable boundary conditions to assess their stability.Furthermore,a case study of the Saint-Alban test embankment A in Quebec,Canada,is provided to demonstrate the applicability of the proposed FE model. 展开更多
关键词 Strength anisotropy Elasto-plastic finite element method(FEM) Three-dimensional(3D)soil slope Gravity increase method(GIM) Stability analysis Case study
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Method of reverberation ray matrix for static analysis of planar framed structures composed of anisotropic Timoshenko beam members 被引量:2
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作者 Jiao ZHANG Guohua NIE 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2015年第2期233-242,共10页
Based on the method of reverberation ray matrix(MRRM), a reverberation matrix for planar framed structures composed of anisotropic Timoshenko(T) beam members containing completely hinged joints is developed for st... Based on the method of reverberation ray matrix(MRRM), a reverberation matrix for planar framed structures composed of anisotropic Timoshenko(T) beam members containing completely hinged joints is developed for static analysis of such structures.In the MRRM for dynamic analysis, amplitudes of arriving and departing waves for joints are chosen as unknown quantities. However, for the present case of static analysis, displacements and rotational angles at the ends of each beam member are directly considered as unknown quantities. The expressions for stiffness matrices for anisotropic beam members are developed. A corresponding reverberation matrix is derived analytically for exact and unified determination on the displacements and internal forces at both ends of each member and arbitrary cross sectional locations in the structure. Numerical examples are given and compared with the finite element method(FEM) results to validate the present model. The characteristic parameter analysis is performed to demonstrate accuracy of the present model with the T beam theory in contrast with errors in the usual model based on the Euler-Bernoulli(EB) beam theory. The resulting reverberation matrix can be used for exact calculation of anisotropic framed structures as well as for parameter analysis of geometrical and material properties of the framed structures. 展开更多
关键词 planar framed structure ANISOTROPIC Timenshenko(T) beam stiffness matrix method of reverberation ray matrix(MRRM) static analysis
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Cracking analysis of fracture mechanics by the finite element method of lines(FEMOL) 被引量:2
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作者 Shaowei Hu Brian Moran 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2005年第5期495-502,共8页
The Finite Element Method of Lines (FEMOL) is a semi-analytic approach and takes a position between FEM and analytic methods. First, FEMOL in Fracture Mechanics is presented in detail. Then, the method is applied to... The Finite Element Method of Lines (FEMOL) is a semi-analytic approach and takes a position between FEM and analytic methods. First, FEMOL in Fracture Mechanics is presented in detail. Then, the method is applied to a set of examples such as edge-crack plate, the central-crack plate, the plate with cracks emanating from a hole under tensile or under combination loads of tensile and bending. Their dimensionless stress distribution, the stress intensify factor (SIF) and crack opening displacement (COD) are obtained, and comparison with known solutions by other methods are reported. It is found that a good accuracy is achieved by FEMOL. The method is successfully modified to remarkably increase the accuracy and reduce convergence difficulties. So it is a very useful and new tool in studying fracture mechanics problems. 展开更多
关键词 The finite element method of lines (FEMOL) .Cracking analysis . Application
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Antibiotic-Resistant Bacterial Group in Field Soil Evaluated by a Newly Developed Method Based on Restriction Fragment Length Polymorphism Analysis 被引量:1
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作者 Katsuji Watanabe Naoto Horinishi Kunimasa Matumoto 《Advances in Microbiology》 2015年第12期807-816,共10页
Spreading of antibiotic resistant bacteria into environment is becoming a major public health problem, implicating affair of the indirect transmission of antibiotic resistant bacteria to human through drinking water, ... Spreading of antibiotic resistant bacteria into environment is becoming a major public health problem, implicating affair of the indirect transmission of antibiotic resistant bacteria to human through drinking water, or vegetables, or daily products. Until now, the risk of nosocomial infection of antibiotic resistant bacteria has mainly been evaluated using clinical isolates by phenotypic method. To evaluate a risk of community-acquired infection of antibiotic resistant bacteria, a new method has been developed based on PCR-RFLP without isolation. By comparing restriction fragment lengths of the 16S rDNA gene from bacterial mixture grown under antibiotic treatment to those simulated from the DNA sequence, bacterial taxonomies were elucidated using the method of Okuda and Watanabe [1] [2]. In this study, taxonomies of polymyxin B resistant bacteria group in field soils, paddy field with organic manure and upland field without organic manure were estimated without isolation. In the both field soils, the major bacteria grown under the antibiotic were B. cereus group, which had natural resistance to this antibiotic. In field applied with organic manure, Prevotella spp., and the other Cytophagales, which were suggested to be of feces origin and to acquire resistance to the antibiotic, were detected. When numbers of each bacterial group were roughly estimated by the most probable number method, B. cereus group was enumerated to be 3.30 × 106 MPN/g dry soil in paddy field soil and 1.32 × 106 MPN/g dry soil in upland filed. Prevotella spp. and the other Cytophagales in paddy field were enumerated to be 1.31 × 106 MPN, and 1.07 × 106 MPN·g-1 dry soil. 展开更多
关键词 POLYMYXIN B Resistant Bacteria Field Soil Microchip ELECTROPHORESIS Multiple Enzyme RESTRICTION fraGMENT Length Polymorphism analysis the Most PROBABLE Number method
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Hydraulic Fracture Parameter Inversion Method for Shale Gas Wells Based on Transient Pressure-Drop Analysis during Hydraulic Fracturing Shut-in Period 被引量:1
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作者 Shangjun Gao Yang Yang +4 位作者 Man Chen Jian Zheng Luqi Qin Xiangyu Liu Jianying Yang 《Energy Engineering》 EI 2024年第11期3305-3329,共25页
Horizontal well drilling and multi-stage hydraulic fracturing are key technologies for the development of shale gas reservoirs.Instantaneous acquisition of hydraulic fracture parameters is crucial for evaluating fract... Horizontal well drilling and multi-stage hydraulic fracturing are key technologies for the development of shale gas reservoirs.Instantaneous acquisition of hydraulic fracture parameters is crucial for evaluating fracturing effectiveness,optimizing processes,and predicting gas productivity.This paper establishes a transient flow model for shale gas wells based on the boundary element method,achieving the characterization of stimulated reservoir volume for a single stage.By integrating pressure monitoring data following the pumping shut-in period of hydraulic fracturing for well testing interpretation,a workflow for inverting fracture parameters of shale gas wells is established.This new method eliminates the need for prolonged production testing and can interpret parameters of individual hydraulic fracture segments,offering significant advantages over the conventional pressure transient analysismethod.The practical application of thismethodology was conducted on 10 shale gaswellswithin the Changning shale gas block of Sichuan,China.The results show a high correlation between the interpreted single-stage total length and surface area of hydraulic fractures and the outcomes of gas production profile tests.Additionally,significant correlations are observed between these parameters and cluster number,horizontal stress difference,and natural fracture density.This demonstrates the effectiveness of the proposed fracture parameter inversion method and the feasibility of field application.The findings of this study aim to provide solutions and references for the inversion of fracture parameters in shale gas wells. 展开更多
关键词 Well test analysis shale gas hydraulic fracturing boundary element method fracturing effect evaluation
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Chemical Analysis Method for Carbon Bearing Refractory Products——Determination of the Total Carbon by Combustion Gravimetric Method 被引量:1
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作者 Shen Keyin 《China's Refractories》 CAS 2007年第1期34-36,共3页
GB/T 13245-91 1 Theme and Scope This standard specifies the method abstract, reagents, apparatus, specimen, analyzing procedure, result calculation and permissible tolerance used for determination of the total carbon ... GB/T 13245-91 1 Theme and Scope This standard specifies the method abstract, reagents, apparatus, specimen, analyzing procedure, result calculation and permissible tolerance used for determination of the total carbon with combustion gravimetric method. 展开更多
关键词 Determination of the Total Carbon by Combustion Gravimetric method Chemical analysis method for Carbon Bearing Refractory Products
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Chemical Analysis Method for Magnesia and Magnesia-Alumina Refractories——Gravimetric-Molybdenum Blue Photometric Method for Determination of Silicon Dioxide Content 被引量:1
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作者 Wang Jing 《China's Refractories》 CAS 2007年第1期39-41,共3页
This standard specifies the gravimetric-molybdenum blue photometric method for determination of silicon dioxide content.
关键词 Chemical analysis method for Magnesia and Magnesia-Alumina Refractories Gravimetric-Molybdenum Blue Photometric method for Determination of Silicon Dioxide Content
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Public Service Satisfaction of Huilongguan Sports and Cultural Park Based on the Importance-Performance Analysis(IPA)Method
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作者 ZHANG Xinti CHEN Xi HAN Juejia 《Journal of Landscape Research》 2025年第4期44-50,共7页
With Beijing Huilongguan Sports and Cultural Park as the research object,this study was conducted to investigate public service satisfaction in the park by the Importance-Performance Analysis(IPA)method.A questionnair... With Beijing Huilongguan Sports and Cultural Park as the research object,this study was conducted to investigate public service satisfaction in the park by the Importance-Performance Analysis(IPA)method.A questionnaire covering six dimensions,including public transportation,sanitation and environment,and supporting facility construction,was designed.A total of 208 valid samples were collected,and SPSS was employed for reliability and validity tests as well as IPA analysis.The findings were as follows:①Visitors were generally quite satisfied with the overall public services in Huilongguan Sports and Cultural Park.②The highest satisfaction levels were observed in sanitation and environment services and the sports and cultural atmosphere,while lower satisfaction was noted for supporting facility construction and public information services.③The advantage enhancement zone includes sanitation and environment services and sports and cultural atmosphere;and the continuous maintenance zone includes public transportation services and security management amd maintenance;the subsequent opportunity zone includes supporting facility construction and public information services;and there are no dimensions in the urgent improvement zone.The study recommends strengthening the service connotations from three aspects:enhancing facilities with sports as the core,optimizing services with a people-centered approach,and upgrading the information platform through technological efficiency.Additionally,a multi-stakeholder collaborative mechanism involving the government in coordinating policy resources,the operator in improving implementation efficiency,and the public participating in supervision and evaluation is proposed to drive the enhancement of public service quality at Huilongguan Sports and Cultural Park. 展开更多
关键词 Importance-Performance analysis (IPA) method SPORTS PARK PUBLIC service SATISFACTION
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Bayesian analysis of Gamow resonances with reduced basis methods:from eigenvector continuation to post-emulation corrections
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作者 Ruo-Yu Cheng Zhi-Cheng Xu 《Nuclear Science and Techniques》 2025年第12期233-243,共11页
To study the uncertainty quantification of resonant states in open quantum systems,we developed a Bayesian framework by integrating a reduced basis method(RBM)emulator with the Gamow coupled-channel(GCC)approach.The R... To study the uncertainty quantification of resonant states in open quantum systems,we developed a Bayesian framework by integrating a reduced basis method(RBM)emulator with the Gamow coupled-channel(GCC)approach.The RBM,constructed via eigenvector continuation and trained on both bound and resonant configurations,enables the fast and accurate emulation of resonance properties across the parameter space.To identify the physical resonant states from the emulator’s output,we introduce an overlap-based selection technique that effectively isolates true solutions from background artifacts.By applying this framework to unbound nucleus ^(6)Be,we quantified the model uncertainty in the predicted complex energies.The results demonstrate relative errors of 17.48%in the real part and 8.24%in the imaginary part,while achieving a speedup of four orders of magnitude compared with the full GCC calculations.To further investigate the asymptotic behavior of the resonant-state wavefunctions within the RBM framework,we employed a Lippmann–Schwinger(L–S)-based correction scheme.This approach not only improves the consistency between eigenvalues and wavefunctions but also enables a seamless extension from real-space training data to the complex energy plane.By bridging the gap between bound-state and continuum regimes,the L–S correction significantly enhances the emulator’s capability to accurately capture continuum structures in open quantum systems. 展开更多
关键词 Uncertainty quantification Reduced basis method Resonance emulator Bayesian analysis Gamow coupled-channel model
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Benefit Analysis and Discussion of the Same Disease in Different Departments of Public Hospitals under the DIP Payment Method
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作者 Shanlan Song Shengshan Zhu Heng Wang 《Journal of Clinical and Nursing Research》 2025年第11期61-67,共7页
Objective:This study aims to explore the benefit analysis of the same disease in different departments of public hospitals under the DIP payment method.Methods:This study is a retrospective analysis that selected clin... Objective:This study aims to explore the benefit analysis of the same disease in different departments of public hospitals under the DIP payment method.Methods:This study is a retrospective analysis that selected clinical data from patients who received treatment in the Department of Orthopedics and the Department of Acupuncture and Moxibustion at our hospital from January 1,2023,to December 31,2023.The study compared the costs of medications,examinations,treatments,laboratory tests,nursing and other expenses,and total treatment costs between the two departments.It analyzed the cost structure of the two departments and proposed further improvement suggestions.Results:The study results indicated that the total costs in the Department of Acupuncture and Moxibustion were significantly higher than those in the Department of Orthopedics.Among medication costs,the total medication costs in the Department of Orthopedics were higher than those in the Department of Acupuncture and Moxibustion,with costs for Western medicine,proprietary Chinese medicine,and herbal medicine all being higher(p<0.05).Regarding examination costs,consultation fees in the Department of Orthopedics were lower than those in the Department of Acupuncture and Moxibustion,while examination costs were higher(p<0.05).In terms of treatment costs,orthopedic treatment and surgical fees were higher than those in the Department of Acupuncture and Moxibustion(p<0.05).For laboratory test costs,orthopedic laboratory fees were significantly higher than those in the Department of Acupuncture and Moxibustion(p<0.05).Among nursing and other expenses,orthopedic blood transfusion,bed fees,and other expenses were higher than those in the Department of Acupuncture and Moxibustion,while nursing fees were lower(p<0.05).Conclusion:Treatment fees in the Department of Acupuncture and Moxibustion are the core and account for a relatively high proportion of the total costs.The benefits generated by the Department of Orthopedics are primarily derived from medication,examination,and laboratory fees,aligning with the characteristics of combining diagnosis,medication,and surgical intervention in orthopedic treatment.Consultation fees,nursing fees,and bed fees in the Department of Acupuncture and Moxibustion are higher than those in the Department of Orthopedics,indicating a longer treatment cycle in acupuncture,which warrants clinical attention. 展开更多
关键词 DIP payment method Public hospitals The same disease Different departments Benefit analysis APPLICATION
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A method to address the challenges of charging conditions on incremental capacity analysis:An ICA-compensation technique incorporating current interrupt methods
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作者 Jinghua Sun Josef Kainz 《Journal of Energy Chemistry》 2025年第9期65-80,I0004,共17页
The incremental capacity analysis(ICA)technique is notably limited by its sensitivity to variations in charging conditions,which constrains its practical applicability in real-world scenarios.This paper introduces an ... The incremental capacity analysis(ICA)technique is notably limited by its sensitivity to variations in charging conditions,which constrains its practical applicability in real-world scenarios.This paper introduces an ICA-compensation technique to address this limitation and propose a generalized framework for assessing the state of health(SOH)of batteries based on ICA that is applicable under differing charging conditions.This novel approach calculates the voltage profile under quasi-static conditions by subtracting the voltage increase attributable to the additional polarization effects at high currents from the measured voltage profile.This approach's efficacy is contingent upon precisely acquiring the equivalent impedance.To obtain the equivalent impedance throughout the batteries'lifespan while minimizing testing costs,this study employs a current interrupt technique in conjunction with a long short-term memory(LSTM)network to develop a predictive model for equivalent impedance.Following the derivation of ICA curves using voltage profiles under quasi-static conditions,the research explores two scenarios for SOH estimation:one utilizing only incremental capacity(IC)features and the other incorporating both IC features and IC sampling.A genetic algorithm-optimized backpropagation neural network(GABPNN)is employed for the SOH estimation.The proposed generalized framework is validated using independent training and test datasets.Variable test conditions are applied for the test set to rigorously evaluate the methodology under challenging conditions.These evaluation results demonstrate that the proposed framework achieves an estimation accuracy of 1.04%for RMSE and 0.90%for MAPE across a spectrum of charging rates ranging from 0.1 C to 1 C and starting SOCs between 0%and 70%,which constitutes a major advancement compared to established ICA methods.It also significantly enhances the applicability of conventional ICA techniques in varying charging conditions and negates the necessity for separate testing protocols for each charging scenario. 展开更多
关键词 Lithium-ion batteries Incremental capacity analysis Charging conditions State of health Current interrupt method
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Sensitivity Analysis of Structural Dynamic Behavior Based on the Sparse Polynomial Chaos Expansion and Material Point Method
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作者 Wenpeng Li Zhenghe Liu +4 位作者 Yujing Ma Zhuxuan Meng Ji Ma Weisong Liu Vinh Phu Nguyen 《Computer Modeling in Engineering & Sciences》 2025年第2期1515-1543,共29页
This paper presents a framework for constructing surrogate models for sensitivity analysis of structural dynamics behavior.Physical models involving deformation,such as collisions,vibrations,and penetration,are devel-... This paper presents a framework for constructing surrogate models for sensitivity analysis of structural dynamics behavior.Physical models involving deformation,such as collisions,vibrations,and penetration,are devel-oped using the material point method.To reduce the computational cost of Monte Carlo simulations,response surface models are created as surrogate models for the material point system to approximate its dynamic behavior.An adaptive randomized greedy algorithm is employed to construct a sparse polynomial chaos expansion model with a fixed order,effectively balancing the accuracy and computational efficiency of the surrogate model.Based on the sparse polynomial chaos expansion,sensitivity analysis is conducted using the global finite difference and Sobol methods.Several examples of structural dynamics are provided to demonstrate the effectiveness of the proposed method in addressing structural dynamics problems. 展开更多
关键词 Structural dynamics DEFORMATION material point method sparse polynomial chaos expansion adaptive randomized greedy algorithm sensitivity analysis
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The Homotopy Analysis Method for Approximating of Giving Up Smoking Model in Fractional Order
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作者 Anwar Zeb M. Ikhlaq Chohan Gul Zaman 《Applied Mathematics》 2012年第8期914-919,共6页
In this paper, we consider the giving up smoking model. First, we present the giving up smoking model in fractional order. Then the homotopy analysis method (HAM) is employed to compute an approximate and analytical s... In this paper, we consider the giving up smoking model. First, we present the giving up smoking model in fractional order. Then the homotopy analysis method (HAM) is employed to compute an approximate and analytical solution of the model in fractional order. The obtained results are compaired with those obtained by forth order Runge-Kutta method and nonstandard numerical method in the integer case. Finally, we present some numerical results. 展开更多
关键词 fraCTIONAL Differential Equations EPIDEMIC MODEL HOMOTOPY analysis method
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Homotopy Analysis Method for a Conservative Nonlinear Oscillator with Fractional Power
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作者 Huaxiong Chen Yanyan Wang 《Journal of Applied Mathematics and Physics》 2021年第1期31-40,共10页
In this paper, the homotopy analysis method is applied to deduce the periodic solutions of a conservative nonlinear oscillator for which the elastic force term is proportional to<em> u</em><sup>1/3&l... In this paper, the homotopy analysis method is applied to deduce the periodic solutions of a conservative nonlinear oscillator for which the elastic force term is proportional to<em> u</em><sup>1/3</sup>. By introducing the auxiliary linear operator and the initial guess of solution, the homotopy analysis solving is set up. By choosing the suitable convergence-control parameter, the accurate high-order approximations of solution and frequency for the whole range of initial amplitudes can easily be obtained. Comparison of the results obtained using this method with those obtained by different methods reveals that the former is more accurate, effective and convenient for these types of nonlinear oscillators. 展开更多
关键词 Homotopy analysis method Approximate Solutions fractional Power Nonlinear Oscillation
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A novel box-counting method for quantitative fractal analysis of threedimensional pore characteristics in sandstone
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作者 Huiqing Liu Heping Xie +2 位作者 Fei Wu Cunbao Li Renbo Gao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第4期479-489,共11页
Fractal theory offers a powerful tool for the precise description and quantification of the complex pore structures in reservoir rocks,crucial for understanding the storage and migration characteristics of media withi... Fractal theory offers a powerful tool for the precise description and quantification of the complex pore structures in reservoir rocks,crucial for understanding the storage and migration characteristics of media within these rocks.Faced with the challenge of calculating the three-dimensional fractal dimensions of rock porosity,this study proposes an innovative computational process that directly calculates the three-dimensional fractal dimensions from a geometric perspective.By employing a composite denoising approach that integrates Fourier transform(FT)and wavelet transform(WT),coupled with multimodal pore extraction techniques such as threshold segmentation,top-hat transformation,and membrane enhancement,we successfully crafted accurate digital rock models.The improved box-counting method was then applied to analyze the voxel data of these digital rocks,accurately calculating the fractal dimensions of the rock pore distribution.Further numerical simulations of permeability experiments were conducted to explore the physical correlations between the rock pore fractal dimensions,porosity,and absolute permeability.The results reveal that rocks with higher fractal dimensions exhibit more complex pore connectivity pathways and a wider,more uneven pore distribution,suggesting that the ideal rock samples should possess lower fractal dimensions and higher effective porosity rates to achieve optimal fluid transmission properties.The methodology and conclusions of this study provide new tools and insights for the quantitative analysis of complex pores in rocks and contribute to the exploration of the fractal transport properties of media within rocks. 展开更多
关键词 3D fractal analysis fractal dimension Rock pore structure Box-counting method Permeability simulation Computational geosciences
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Q-homotopy analysis method for time-fractional Newell–Whitehead equation and time-fractional generalized Hirota–Satsuma coupled KdV system
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作者 Di Liu Qiongya Gu Lizhen Wang 《Communications in Theoretical Physics》 SCIE CAS CSCD 2024年第3期70-83,共14页
In this paper,two types of fractional nonlinear equations in Caputo sense,time-fractional Newell–Whitehead equation(FNWE)and time-fractional generalized Hirota–Satsuma coupled KdV system(HS-cKdVS),are investigated b... In this paper,two types of fractional nonlinear equations in Caputo sense,time-fractional Newell–Whitehead equation(FNWE)and time-fractional generalized Hirota–Satsuma coupled KdV system(HS-cKdVS),are investigated by means of the q-homotopy analysis method(q-HAM).The approximate solutions of the proposed equations are constructed in the form of a convergent series and are compared with the corresponding exact solutions.Due to the presence of the auxiliary parameter h in this method,just a few terms of the series solution are required in order to obtain better approximation.For the sake of visualization,the numerical results obtained in this paper are graphically displayed with the help of Maple. 展开更多
关键词 fractional Newell-Whitehead equation fractional generalized Hirota-Satsuma coupled KdV system approximate solution q-homotopy analysis method
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Bacterial Groups Concerned with Maturing Process in Manure Production Analyzed by a Method Based on Restriction Fragment Length Polymorphism Analysis
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作者 Katsuji Watanabe Naoto Horinishi +2 位作者 Kunimasa Matumoto Akihiro Tanaka Kenichi Yakushido 《Advances in Microbiology》 2015年第13期832-841,共10页
Composting is a biological aerobic decomposition process consisted from different phases. Although the Japanese Standards for manure recommended that it took at least 6 months to complete the maturing phase, there was... Composting is a biological aerobic decomposition process consisted from different phases. Although the Japanese Standards for manure recommended that it took at least 6 months to complete the maturing phase, there was no reliable ground. In order to find out shortening method of the maturing phase, the microorganisms concerned with a progress of the maturing was determined by using the most probable number method (MPN) and PCR-RFLP of the 16S rDNA, which was found effective to provide numbers and taxonomy of polymyxin B resistant bacterial groups in the former paper [1]. Compared to the numbers after thermophilic phase, those of Actinobacteria, δ-proteobacteria, and the other gram negative bacteria increased to 50 times, 20 times, and 105 times, respectively, after maturing phase, while those of Bacillus spp., and α and β-proteobacteria decreased to 1/10, and 1/105 after maturing phase. Numbers of the other Fumicutes, and γ-proteobacteria remained in the same revel. Actinobacteria, δ-proteobacteria, and the other gram negative bacteria might be concerned with a progress of the maturing phase, because these bacterial groups were detected and enumerated due to their proliferation ability. Although number of Acitinobacteria might be underestimated because of a PCR bias, the method was found effective for the purpose to monitor bacteria actively proliferated in culture medium. 展开更多
关键词 Maturing Phase MANURE PRODUCTION Microchip ELECTROPHORESIS Multiple Enzyme RESTRICTION fragment Length Polymorphism analysis The Most PROBABLE Number method
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Chemical Analysis Method for Carbon Bearing Refractory Products——Determination of Magnesium Oxide Content by CyDTA Volumetric Method
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作者 Zhang Xiaohui 《China's Refractories》 CAS 2007年第1期37-38,共2页
This standard specifies the method summary, reagents, apparatus, sampling, procedure, test results calculation and permissible tolerance of the determination of magnesium oxide by CyDTA volumetric method.
关键词 Determination of Magnesium Oxide Content by CyDTA Volumetric method TEST Chemical analysis method for Carbon Bearing Refractory Products
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Evaluation of the Method Based on Restriction Fragment Length Polymorphism Analysis as Simple Analysis Method of Lactic Acid Bacteria in Foods
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作者 Kunimasa Matsumoto Kouya Shimada +1 位作者 Naoto Horinishi Katsuji Watanabe 《Food and Nutrition Sciences》 2016年第3期163-172,共10页
Lactic acid bacteria have not only been used to produce various kinds of fermented food, but also used as probiotic products. As lactic acid bacterial group was consisted from diverse genera, a simple inspection metho... Lactic acid bacteria have not only been used to produce various kinds of fermented food, but also used as probiotic products. As lactic acid bacterial group was consisted from diverse genera, a simple inspection method by which numbers and contained microorganisms could be automatically analyzed without any preliminary information was required to use them more effectively. In this manuscript, lactic acid bacterial groups in commercial products of kimuchi, komekouji-miso, and yoghurt were identified and enumerated by our newly developed method [1]-[3], to evaluate whether the method could be used as an inspection method of various food samples. In kimuchi, numerically dominant bacteria were Lactobacillus sakei, and L. casei (1.4 × 104 MPN g<sup>-1</sup>) and Leuconostoc spp. (l.4 × 104 MPN). In kouji-miso, numerically dominant bacteria was Bacillus spp. (3 × 103 MPN), which mainly included B. subtilis group and B. cereus group. Lactic acid bacteria such as Lactobacillus spp., or Lactococcus spp., included in the komekouji-miso, could be enumerated after 3 days incubation (1.24 × 104 MPN), but not detected after 7 days incubation. In yoghurt A and C, Lactococcus lactis was detected as numerically dominant lactic acid bacteria (3.0 × 105 MPN). In yoghurt B, Lactobacillus spp., or Lactococcus spp., was detected not only by a culturebased method but also by an unculture-based method, although there was a difference between the both estimated numbers. The present results suggested that the method might become useful as a simple inspection method of food microorganisms, because time and labor of the analysis could be reduced by using an unculture-based method and MCE-202 MultiNA. In this study, Bifidobacteriium spp. was not detected in B and C yoghurt, in spite of indicating their existence, and numbers of lactic acid bacteria were lower than the level of the daily product regulation, because 16S rDNA of Bifidobacteriium spp. might not be amplified by the used PCR condition. The PCR condition must be changed so as to amplify Bifidobacterium spp., before the method will be used as an inspection method for lactic acid bacteria. 展开更多
关键词 Multiple Enzyme Restriction fragment Length Polymorphism analysis Most Probable Number method Lactic Acid Bacteria Komekouji-Miso Kimuchi YOGHURT
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