Porous microneedles have attracted considerable attention as minimally invasive tools for interstitial fluid samplingand biomarker analyses.However,existing porous microneedle fabrication methods often suffer from low...Porous microneedles have attracted considerable attention as minimally invasive tools for interstitial fluid samplingand biomarker analyses.However,existing porous microneedle fabrication methods often suffer from low extractionefficiency,primarily because of the inherent trade-off between increasing porosity and maintaining sufficientmechanical strength.Herein,we present a novel approach for fabricating porous microneedles with controllable poresizes and enhanced extraction performance.Monodisperse polylactic acid microspheres,produced via microfluidictechniques,are thermally bonded to form porous microneedles with interconnected pore networks originating fromthe connected voids between the microspheres.By precisely adjusting the microsphere diameter,we optimize thepore size to achieve high extraction efficiency while preserving structural integrity.Following surface treatment andbonding parameter optimization,the resulting porous microneedles exhibit sufficient mechanical strength topenetrate human skin and achieve an in vitro extraction rate of 0.95μL/min per needle—the highest reported to date.Furthermore,porous microneedles are integrated with a colorimetric paper-based sensor for glucose detection,demonstrating a linear correlation between glucose concentration and the colorimetric response of the sensor.Thiswork provides a promising tool for high-speed interstitial fluid extraction and expands the fabrication strategy forporous structures in biosensing applications.展开更多
基金funded and supported by Japan Science and Technology Agency SPRING(Grant number:JPMJSP2108)Japan,and Japan Society for the Promotion of Science Core-to-Core Program A(Grant number:JSPSCCA20190006)supported by 2025 Hyper-Convergence Research Support Program(0681-20250036)at Seoul National University.
文摘Porous microneedles have attracted considerable attention as minimally invasive tools for interstitial fluid samplingand biomarker analyses.However,existing porous microneedle fabrication methods often suffer from low extractionefficiency,primarily because of the inherent trade-off between increasing porosity and maintaining sufficientmechanical strength.Herein,we present a novel approach for fabricating porous microneedles with controllable poresizes and enhanced extraction performance.Monodisperse polylactic acid microspheres,produced via microfluidictechniques,are thermally bonded to form porous microneedles with interconnected pore networks originating fromthe connected voids between the microspheres.By precisely adjusting the microsphere diameter,we optimize thepore size to achieve high extraction efficiency while preserving structural integrity.Following surface treatment andbonding parameter optimization,the resulting porous microneedles exhibit sufficient mechanical strength topenetrate human skin and achieve an in vitro extraction rate of 0.95μL/min per needle—the highest reported to date.Furthermore,porous microneedles are integrated with a colorimetric paper-based sensor for glucose detection,demonstrating a linear correlation between glucose concentration and the colorimetric response of the sensor.Thiswork provides a promising tool for high-speed interstitial fluid extraction and expands the fabrication strategy forporous structures in biosensing applications.
