[Objective] The study aimed at cloning and identifying the toll receptor gene 9（TLR9） of wild Ovis ammon in Xinjiang,and predicting its structure and function.[Method] The TLR9 complete sequence of wild Ovis ammon w...[Objective] The study aimed at cloning and identifying the toll receptor gene 9（TLR9） of wild Ovis ammon in Xinjiang,and predicting its structure and function.[Method] The TLR9 complete sequence of wild Ovis ammon was cloned from its peripheral blood by PCR technology.Then the PCR products were purified by agarose gel electrophoresis and then sequenced.Finally,the structure and function of TLR9 sequence were predicted by molecular biological software.[Result] The complete sequence of TLR9 gene was 3 192 bp in length,encoding 1 064 amino acids with a signal peptide composed of 30 amino acids;the leucine percentage reached as high as 18.5%.The TLR9 amino acid possibly contained three hydrophobic regions,at amino acids 455-475,740-760 and 780-800.The 3-D structure of TLR9 was constructed by the extracellular LRR domain and intracellular TIL domain（Toll/IL IR）.[Conclusion] The characteristics of TLR9 provided theoretical basis for further study on the TLR9 gene of wild Ovis ammon in Xinjiang.展开更多
Ammonium carnallite was synthesized by using MgCl2 and NH4Cl,which obtained by the saline lake bischofite and filtrate stock of deposit magnesium by ammonia respectively,at molar ratio of 1:1.Then the ammonium carnall...Ammonium carnallite was synthesized by using MgCl2 and NH4Cl,which obtained by the saline lake bischofite and filtrate stock of deposit magnesium by ammonia respectively,at molar ratio of 1:1.Then the ammonium carnallite had been dehydrated to some extent at 160℃ for 4h.The dehydrated ammonium carnallite was dissolved in methanol under low temperature by feeding ammonia,preparing ammoniation magnesium chloride.The kinetics of non-isothermal decomposition was studied for ammoniation magnesium chloride.The process of ammoniation magnesium chloride to eliminate ammonia in the temperature range 78～246℃ can be described by the model of Ginstling-Brounshtein {f(α)=1.5[(1-α)-1/3-1]-1,g(α)=(1-2α/3)-(1-α)2/3}.The kinetic equation is dα/dt= 1.5Ae-E/RT [(1-α)-1/3-1]-1.The processes of ammoniation magnesium chloride to eliminate ammonia within 246～299℃ can be described by the model of Zhuralev,Lesoki and Tempelman {f(α)= 1.5(1-α)4/3[(1-α)-1/3-1]-1,g(α)= [(1-α)-1/3-1]2}.The kinetic equation is dα/dt= 1.5Ae-E/RT(1-α)4/3[(1-α)-1/3-1]-1.The kinetics equations and parameters provided valuable data for ammonition magnesium chloride to produced anhydrous magnesium chloride.展开更多
文摘[Objective] The study aimed at cloning and identifying the toll receptor gene 9（TLR9） of wild Ovis ammon in Xinjiang,and predicting its structure and function.[Method] The TLR9 complete sequence of wild Ovis ammon was cloned from its peripheral blood by PCR technology.Then the PCR products were purified by agarose gel electrophoresis and then sequenced.Finally,the structure and function of TLR9 sequence were predicted by molecular biological software.[Result] The complete sequence of TLR9 gene was 3 192 bp in length,encoding 1 064 amino acids with a signal peptide composed of 30 amino acids;the leucine percentage reached as high as 18.5%.The TLR9 amino acid possibly contained three hydrophobic regions,at amino acids 455-475,740-760 and 780-800.The 3-D structure of TLR9 was constructed by the extracellular LRR domain and intracellular TIL domain（Toll/IL IR）.[Conclusion] The characteristics of TLR9 provided theoretical basis for further study on the TLR9 gene of wild Ovis ammon in Xinjiang.
文摘Ammonium carnallite was synthesized by using MgCl2 and NH4Cl,which obtained by the saline lake bischofite and filtrate stock of deposit magnesium by ammonia respectively,at molar ratio of 1:1.Then the ammonium carnallite had been dehydrated to some extent at 160℃ for 4h.The dehydrated ammonium carnallite was dissolved in methanol under low temperature by feeding ammonia,preparing ammoniation magnesium chloride.The kinetics of non-isothermal decomposition was studied for ammoniation magnesium chloride.The process of ammoniation magnesium chloride to eliminate ammonia in the temperature range 78～246℃ can be described by the model of Ginstling-Brounshtein {f(α)=1.5[(1-α)-1/3-1]-1,g(α)=(1-2α/3)-(1-α)2/3}.The kinetic equation is dα/dt= 1.5Ae-E/RT [(1-α)-1/3-1]-1.The processes of ammoniation magnesium chloride to eliminate ammonia within 246～299℃ can be described by the model of Zhuralev,Lesoki and Tempelman {f(α)= 1.5(1-α)4/3[(1-α)-1/3-1]-1,g(α)= [(1-α)-1/3-1]2}.The kinetic equation is dα/dt= 1.5Ae-E/RT(1-α)4/3[(1-α)-1/3-1]-1.The kinetics equations and parameters provided valuable data for ammonition magnesium chloride to produced anhydrous magnesium chloride.
