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Long-term manure amendment enhances N_(2)O emissions from acidic soil by alleviating acidification and increasing nitrogen mineralization
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作者 Lei Wu Jing Hu +4 位作者 Muhammad Shaaban Jun Wang Kailou Liu Minggang Xu Wenju Zhang 《Journal of Integrative Agriculture》 2026年第1期262-272,共11页
Long-term manure application has the potential to alleviate soil acidification, and increase carbon sequestration and nutrient availability, thus improving cropland fertility. However, the mechanisms behind greenhouse... Long-term manure application has the potential to alleviate soil acidification, and increase carbon sequestration and nutrient availability, thus improving cropland fertility. However, the mechanisms behind greenhouse gas N_(2)O emissions from acidic soil mediated by long-term manure application remain poorly understood. Herein, we investigated N_(2)O emission and its linkage with gross N mineralization and nitrification rates, as well as nitrifying and denitrifying microbes in an acidic upland soil subjected to 36-year fertilization treatments, including an unfertilized control(CK), inorganic fertilizer(F), 2× rate of inorganic fertilizer(2F), manure(M), and the combination of inorganic fertilizer and manure(FM) treatments. Compared to the CK treatment(1.34 μg N kg^(-1) d^(-1)), fertilization strongly increased N_(2)O emissions by 34-fold on average, with more pronounced increases in the manure-amendment(10.6-169 μg N kg^(-1) d^(-1)) than those in the inorganic fertilizer treatments(3.26-5.51 μg N kg^(-1) d^(-1)). The manure amendment-stimulated N_(2)O emissions were highly associated with increased soil pH, mean weight diameter of soil aggregates, substrate availability(e.g., particulate organic carbon, NO_(3)^(-)and available phosphorus), gross N mineralization rates, denitrifier abundances and the(nirK+nirS)/nosZ ratio. These findings suggest that the increased N_(2)O emissions primarily resulted from alleviated acidification, increased substrate availability and improved soil structure, thus enhancing microbial N mineralization and favoring N_(2)O^(-)producing denitrifiers over N_(2)O consumers. Moreover, ammonia-oxidizing bacteria(AOB) rather than ammonia-oxidizing archaea(AOA) positively correlated with soil NO_(3)^(-)concentration and N_(2)O emissions, indicating that nitrification indirectly contributed to N_(2)O production by supplying NO_(3)^(-)for denitrification. Collectively, manure amendment potentially stimulates N_(2)O emissions, primarily resulting from alleviated soil acidification and increased substrate availability, thus enhancing N mineralization and denitrifier-mediated N_(2)O production. Our findings suggest that consideration should be given to the greenhouse gas budgets of agricultural ecosystems when applying manure for managing the pH and fertility of acidic soils. 展开更多
关键词 long-term manure amendment N_(2)O emission nitrogen mineralization denitrification (nirK+nirS)/nosZ
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Impacts of Fertilization and Soil Amendments on Rhizosphere Microbiota and Growth of Panax:A Meta-Analysis
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作者 Hong Chen Runze Yang +4 位作者 Jing Tian Boyuan Xu Qiang Chen Yuzong Chen Ming-Xiao Zhao 《Phyton-International Journal of Experimental Botany》 2026年第1期187-202,共16页
Panax species are globally recognized for their high medicinal and economic value,yet large-scale cultivation is constrained by high production costs,progressive soil acidification,and persistent soil-borne diseases.A... Panax species are globally recognized for their high medicinal and economic value,yet large-scale cultivation is constrained by high production costs,progressive soil acidification,and persistent soil-borne diseases.Although various soil improvement strategies have been tested,a comprehensive synthesis of their comparative effectiveness has been lacking.Here,we conducted a meta-analysis of 1381 observations from 54 independent studies to evaluate the effects of conventional fertilizers,microbial fertilizers,organic amendments,and inorganic amendments on Panax cultivation.Our results demonstrate that microbial fertilizers,organic amendments,and inorganic amendments significantly increased soil pH,thereby ameliorating soil acidification.Among them,organic amendments significantly enhanced the content of soil organic carbon,available nitrogen,and available phosphorus,alongside a notable increase in microbial diversity(Chao1 and ACE indices,which increased by 9%and 17%,respectively).Moreover,our analysis revealed that while microbial fertilizers,organic amendments,and inorganic amendments(except conventional fertilizers)reduced the disease index of Panax plants,organic amendments demonstrated absolute superiority in promoting plant height,root dry weight,root fresh weight,and root length.By quantitatively integrating multi-source evidence,this study provides novel mechanistic insights and practical recommendations that extend beyond local practices,offering guidance for sustainable ginseng cultivation and broader medicinal plant production systems worldwide. 展开更多
关键词 Panax cultivation soil amendments fertilizers soil bacteria
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Different extractable pools of Cd and Pb in agricultural soil under amendments:Water-soluble concentration sensitively indicates metal availability 被引量:1
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作者 Zidi Wang Wenyao Tang +8 位作者 Xiaodong Ding Qiang Dong Yingying Guo Guangliang Liu Yanwei Liu Yong Liang Yongguang Yin Yong Cai Guibin Jiang 《Journal of Environmental Sciences》 2025年第4期297-308,共12页
Identification of the most appropriate chemically extractable pool for evaluating Cd and Pb availability remains elusive,hindering accurate assessment on environmental risks and effectiveness of remediation strategies... Identification of the most appropriate chemically extractable pool for evaluating Cd and Pb availability remains elusive,hindering accurate assessment on environmental risks and effectiveness of remediation strategies.This study evaluated the feasibility of European Community Bureau of Reference(BCR)sequential extraction,Ca(NO_(3))_(2)extraction,and water extraction on assessing Cd and Pb availability in agricultural soil amended with slaked lime,magnesium hydroxide,corn stover biochar,and calcium dihydrogen phosphate.Moreover,the enriched isotope tracing technique(^(112)Cd and^(206)Pb)was employed to evaluate the aging process of newly introduced Cd and Pbwithin 56 days’incubation.Results demonstrated that extractable pools by BCR and Ca(NO_(3))_(2)extraction were little impacted by amendments and showed little correlation with soil pH.This is notable because soil pH is closely linked to metal availability,indicating these extraction methods may not adequately reflect metal availability.Conversely,water-soluble concentrations of Cd and Pb were markedly influenced by amendments and exhibited strong correlations with pH(Pearson’s r:-0.908 to-0.825,P<0.001),suggesting water extraction as a more sensitive approach.Furthermore,newly introduced metals underwent a more evident aging process as demonstrated by acid-soluble and water-soluble pools.Additionally,water-soluble concentrations of essential metals were impacted by soil amendments,raising caution on their potential effects on plant growth.These findings suggest water extraction as a promising and attractive method to evaluate Cd and Pb availability,which will help provide assessment guidance for environmental risks caused by heavy metals and develop efficient remediation strategies. 展开更多
关键词 Heavy metals Water-soluble concentrations Enriched stable isotopes Soil amendments Sequential extraction Soil pH
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Mechanistic insights into N_(2)O emission mitigation by nitrification inhibitor dicyandiamide(DCD)in a tropical sandy soil after six years of manure amendment
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作者 Changhua FAN Danfeng WANG +7 位作者 Pengpeng DUAN Wenlong GAO Yuqin LIU Xiaolong WU Huiran LIU Ziyu NING Qinfen LI Miao CHEN 《Pedosphere》 2025年第3期449-461,共13页
Organic amendments(OM)can profoundly affect soil nitrous oxide(N_(2)O)emissions via changing nitrogen(N)cycles.However,mechanistic insights into how nitrification inhibitors modulate the responses of soil N_(2)O emiss... Organic amendments(OM)can profoundly affect soil nitrous oxide(N_(2)O)emissions via changing nitrogen(N)cycles.However,mechanistic insights into how nitrification inhibitors modulate the responses of soil N_(2)O emissions to successive applications of OM are currently insufficient.In this study,we performed a laboratory experiment to examine N_(2)O emissions from a tropical vegetable soil subjected to six years of chemical fertilization(CF)and chemical fertilization combined with manure application(CFM)and evaluate the mitigation effectiveness of nitrification inhibitor dicyandiamide(DCD)under each management regime.Isotopocule mapping showed that bacterial nitrification and/or fungal denitrification accounted for 77.4%–88.5%of total N_(2)O production across treatments during the emission peak.The cumulative N_(2)O emissions from the CFM-treated soil were nearly 8-fold of those from the CF-treated soil.The CFM treatment stimulated N_(2)O production from bacterial nitrification and denitrification by increasing the abundance of genes linked to nitrifiers(ammonia-oxidizing bacterial(AOB)amoA and total comammox amoA)and denitrifiers(nirK,nirS,and qnorB),respectively.Importantly,DCD decreased cumulative N_(2)O emissions by an average of 73.3%,with better mitigation performance observed in the CFM-treated soil than in the CF-treated soil due to stronger inhibited nitrification and increased abundance of the nosZ gene,and altered bacterial community composition.The 16S rRNA sequencing further revealed that adding DCD to the CFM-treated soil resulted in declines in the abundances of bacterial phylum Actinobacteria and Chloroflexi that positively affected N_(2)O emissions;the opposite pattern prevailed for Gemmatimonadetes that negatively affected N_(2)O emissions.This study highlights the potential of manure application,when coupled with nitrification inhibitors,to achieve the dual goals of enhancing soil fertility and reducing environmental risk associated with N_(2)O emissions in tropical agricultural soils. 展开更多
关键词 greenhouse gas high-throughput sequencing isotopocule mapping tropical vegetable soil organic amendment
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Response of abundant and rare microbial taxa to three iron-carbon composite amendments in metal-contaminated agricultural soil
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作者 Ting Yang Jiacan Li +6 位作者 Ying Yuan Xin Zheng Yifei Liu Bing Zhang Tan Chen Jun Jin Linlan Zhuang 《Journal of Environmental Sciences》 2025年第11期170-185,共16页
Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundan... Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies. 展开更多
关键词 Metal-contaminated farmland Iron-carbon composite amendments Abundant taxa Rare taxa Community assembly Ecological functions
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Effects of Lignite Humic Acid and Lignite Humic Acid-based Combined Amendment on Soil Quality in Saline-sodic Farmlands in the West Liaohe Plain,China
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作者 NIE Zhaoyang ZHANG Lu +6 位作者 ZHANG Tieyi GUO Liangliang ZHOU Jie AN Fenghua MA Hongyuan WANG Zhichun YANG Fan 《Chinese Geographical Science》 2025年第2期401-414,共14页
Water scarcity and soil salinization pose significant challenges to agriculture in the West Liaohe Plain,eastern Inner Mongolia,China.Shallow-buried drip irrigation can improve soil water use efficiency to alleviate w... Water scarcity and soil salinization pose significant challenges to agriculture in the West Liaohe Plain,eastern Inner Mongolia,China.Shallow-buried drip irrigation can improve soil water use efficiency to alleviate water shortage in agriculture and the application of lignite humic acid reduces the adverse effects of soil salinization.However,further research is needed to investigate the effects of different application rates of lignite humic acid and humic acid-based combined amendment on soil physicochemical properties,nutrient contents,and crop yield in saline-sodic farmlands under shallow-buried drip irrigation.A two-year field experiment was conducted with control without any amendment(CK),three treatments amended with 3 t/ha(H1),6 t/ha(H2),and 12 t/ha(H3)lignite humic acid,and three application rates with 15 t/ha(T1),22.5 t/ha(T2),and 30 t/ha(T3)lignite humic acid-based combined amendment in 2021 and2022.The results showed that H3 reduced soil bulk density,p H,electrical conductivity,and total alkalinity,while increasing the contents of soil organic matter,total nitrogen,and available potassium in the two-year experiment.Moreover,the maize yield in H3 increased by an average of 35.5%.T2 decreased soil bulk density,p H,total alkalinity,and increased maize yield by 16.2%,compared to the first year.These results suggest that T2 consistently improved both soil quality and crop yield.Correlation analyses showed that lignite humic acid and its complexes promote maize growth and increase yield by increasing soil organic matter and total nitrogen while reducing soil salinity and total alkalinity.Based on the comprehensive analysis of the field data and the results of the comprehensive evaluation of soil quality,it was determined that the appropriate improvement measures for saline-sodic farmlands under shallow-buried drip irrigation are the application of 12 t/ha of lignite humic acid and 22.5 t/ha of lignite humic acid-based combined amendment.This study demonstrates the effectiveness of lignite humic acid and its combined amendment in mitigating the constraints of saline-sodic farmlands and enhancing crop yields,providing a sustainable solution for improving saline-sodic farmlands in the West Liaohe Plain. 展开更多
关键词 lignite humic acid combined amendment soil quality the West Liaohe Plain China
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有机肥复合聚丙烯酰胺对铁尾矿的改良效果
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作者 海龙 许毅 +3 位作者 魏驰文 姜永丰 孙殿兴 孙瑞珩 《水土保持通报》 北大核心 2026年第1期22-28,114,共8页
[目的]探究有机肥和聚丙烯酰胺(PAM)按不同比例配制的改良剂对铁尾矿改良效果的影响,为铁尾矿的改良提供科学依据。[方法]通过室内土培试验和盆栽试验,设计有机肥(5%,6%,7%)和聚丙烯酰胺添加比例(0.05%,0.10%,0.15%)结合室内理化性质分... [目的]探究有机肥和聚丙烯酰胺(PAM)按不同比例配制的改良剂对铁尾矿改良效果的影响,为铁尾矿的改良提供科学依据。[方法]通过室内土培试验和盆栽试验,设计有机肥(5%,6%,7%)和聚丙烯酰胺添加比例(0.05%,0.10%,0.15%)结合室内理化性质分析,同时选用狗牙根(Cynodon dactylon)作为先锋植物,分析植株的生长指标,探究不同有机肥和聚丙烯酰胺添加比例下对铁尾矿的改良效果。[结果](1)添加改良剂能够显著提高尾矿的养分,改善尾矿的pH值,相较于对照组有机质提高71%~179%。(2)不同配比改良剂的加入均降低了尾矿的渗透系数,改善了尾矿的结构,提高了尾矿的保水保肥能力。(3)改良剂的加入显著提升植株发芽率,最高增幅达60%。并且促进株高与根长生长,株高与根长分别增加140%~290%和98%~247%。[结论]有机肥和聚丙烯酰胺的加入能够改善尾矿的理化性质,研究推荐的最优配比方案为:铁尾矿∶有机肥∶聚丙烯酰胺=100∶6∶0.1。 展开更多
关键词 土壤改良剂 有机肥 铁尾矿 聚丙烯酰胺 理化性质
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《民事案件案由规定》(2025年)的理解与适用
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作者 周加海 司艳丽 +1 位作者 贾玉慧 邓楠 《中国应用法学》 2026年第1期23-34,共12页
为贯彻落实党的二十届四中全会精神,聚焦“十五五”时期经济社会发展主要目标,落实《人民法院第六个五年改革纲要(2024—2028年)》第29条“健全完善案件、案号、案由体系以及精准识别工作机制”的要求,最高人民法院对2020年12月29日第... 为贯彻落实党的二十届四中全会精神,聚焦“十五五”时期经济社会发展主要目标,落实《人民法院第六个五年改革纲要(2024—2028年)》第29条“健全完善案件、案号、案由体系以及精准识别工作机制”的要求,最高人民法院对2020年12月29日第二次修正的《民事案件案由规定》作了修改。本次修改遵循严格依法原则、必要性原则、实用性原则。修改后的《民事案件案由规定》共有第一级案由12个,第二级案由59个,第三级案由514个,第四级案由470个,总计1055个案由。本文对《最高人民法院关于修改〈民事案件案由规定〉的决定》《最高人民法院关于印发〈民事案件案由规定〉的通知》(以下统称《修改决定》)的制定背景、基本原则、主要内容、案由规则以及适用时需要注意的问题作一介绍,以便广大法院干警准确理解和适用。 展开更多
关键词 民事案件 案由 修改决定 适用规则 设计思路
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Effect of amendments on growth and metal uptake of giant reed(Arundo donax L.) grown on soil contaminated by arsenic,cadmium and lead 被引量:6
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作者 杨淼 肖细元 +2 位作者 苗旭峰 郭朝晖 王凤永 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第6期1462-1469,共8页
The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown o... The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic (As), cadmium (Cd) and lead (Pb) were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system. 展开更多
关键词 PHYTOREMEDIATION giant reed soil amendments heavy metal contaminated soil metal uptake
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Effects of Soil Amendment on Growth,Yield and Output of Flue-cured Tobacco in South Anhui Province 被引量:2
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作者 潘金华 庄舜尧 +3 位作者 史学正 曹志洪 蔡宪杰 程森 《Agricultural Science & Technology》 CAS 2015年第12期2682-2687,2702,共7页
[Objective] The aim of this study was to evaluate the effects of soil amend- ment composed of biochar and inorganic mineral material on growth and develop- ment, yield and output value of flue-cured tobacco in South A... [Objective] The aim of this study was to evaluate the effects of soil amend- ment composed of biochar and inorganic mineral material on growth and develop- ment, yield and output value of flue-cured tobacco in South Anhui Province. [Method] A field plot experiment was conducted. The agronomic traits, yield and output value, as well as appearance quality and flavor of flue-cured tobacco were evaluated. [Re- suit] The soil amendments composed of biochars and inorganic mineral materials could significantly increase plant height, stem diameter, leaf size, yield and output of flue-cured tobacco, as well as proportion of first-grade tobacco leaves at the middle and late growth period. The yield in the X3 treatment group (70% T20 + 30% ZC) was highest, and it was higher than that in the control group by 398 kg/hm2. Com- pared with that in the control group, the output of flue-cured tobacco in the X3 treatment group was increased by 10 290 yuan/hm2. In terms of appearance quality and flavor, the flue-cured tobacco leaves in the soil amendment treatment groups were all better than those in the control group. [Conclusion] The application of soil amendment composed of biochar and inorganic mineral material is an effective mea- sure to improve tobacco-growing soil, promote tobacco growth and development, im- prove tobacco yield and output and improve tobacco leaf quality in South Anhui re- gion. 展开更多
关键词 Flue-cured tobacco Soil amendment Agronomic traits Yield and output Appearance quality Smoke panel test
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餐余垃圾与玉米淀粉基餐盒共热解特性及产物性质研究
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作者 解海卫 秦雨豪 +2 位作者 张艳 赵伟 闫文弢 《节能与环保》 2026年第2期56-63,共8页
针对餐余垃圾(KW)与玉米淀粉基餐盒(CSB)混合废弃后处理难度大、资源利用效率低的问题,本研究采用热重分析与管式热解炉,系统研究了餐余垃圾与玉米淀粉基餐盒在不同掺混比下的热解失重行为与三相产物特性,并表征所得生物炭的理化性质。... 针对餐余垃圾(KW)与玉米淀粉基餐盒(CSB)混合废弃后处理难度大、资源利用效率低的问题,本研究采用热重分析与管式热解炉,系统研究了餐余垃圾与玉米淀粉基餐盒在不同掺混比下的热解失重行为与三相产物特性,并表征所得生物炭的理化性质。结果表明,餐余垃圾中的碱金属元素具备显著催化作用,使起始分解温度从151.3℃降至142.3℃。随着餐余垃圾比例增加,固、液相产率分别升至26.6%和35.98%,实现了碳元素定向固存;制备的生物炭表面粗糙多孔,富含石英、方解石等矿物,pH值呈强碱性,达10-13,电导率显著升高。该技术实现了矿物元素在生物炭中的富集,所得碱性生物炭具有优良的酸性土壤改良潜力,为城市固废资源化提供了理论依据。 展开更多
关键词 餐余垃圾 玉米淀粉基餐盒 共热解 生物炭 土壤改良
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新治安管理处罚法中与刑法有关的若干问题
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作者 黄京平 《北京联合大学学报(人文社会科学版)》 2026年第1期62-78,共17页
广义的治安管理处罚法规制的违反治安管理行为,是构建“治安违法—刑事犯罪”二元制裁制度的规范基础。在“治安处罚—刑罚处罚”的二元制裁制度中,治安管理处罚法的集中修订和分散修订,都是刑法修正的特别方式,作用在于落实宽严相济刑... 广义的治安管理处罚法规制的违反治安管理行为,是构建“治安违法—刑事犯罪”二元制裁制度的规范基础。在“治安处罚—刑罚处罚”的二元制裁制度中,治安管理处罚法的集中修订和分散修订,都是刑法修正的特别方式,作用在于落实宽严相济刑事政策和终止积极刑法观的立法实践进程。法定违法行为与法定犯对接的最典型规定,实际是分层前置法和双层保障法构成的严控法律追责程度的制度。新治安管理处罚法的实施,要求重新定位治安管理处罚法与刑法的关系,应由“入罪优先、违法兜底”转变为“违法认定优先、严格限制入罪”。新治安管理处罚法,以社会治安综合治理方针、“教惩结合”政策和广义治安调解处理制度等为基础,初步形成了犯罪治理规范体系,具有了“犯罪治理法”的特性;治罪与治理并重,是刑法作为“犯罪惩治法”和治安管理处罚法作为“犯罪治理法”的并重。入罪判断必须为治安管理处罚法预留法定的适用空间,是统一公检法机关执法司法办案标准的一般规则。 展开更多
关键词 治安管理处罚法 二元制裁 刑法修正 限制入罪 社会治安综合治理 教育与处罚相结合 犯罪治理 危险作业 盗窃
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两种典型环境改良剂作用下河蟹肠道菌群的动态变化
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作者 陈友明 李建刚 +1 位作者 周军 董元华 《生态与农村环境学报》 北大核心 2026年第1期102-112,共11页
为评价过硫酸氢钾复合盐(potassium monopersulfate compound)和枯草芽孢杆菌(Bacillus subtilis)两种环境改良剂在河蟹(Eriocheir sinensis)养殖中的生态作用,本研究设置4个实验组,分别为对照组(未添加环境改良剂)、处理A(添加过硫酸... 为评价过硫酸氢钾复合盐(potassium monopersulfate compound)和枯草芽孢杆菌(Bacillus subtilis)两种环境改良剂在河蟹(Eriocheir sinensis)养殖中的生态作用,本研究设置4个实验组,分别为对照组(未添加环境改良剂)、处理A(添加过硫酸氢钾复合盐)、处理B(添加枯草芽孢杆菌)和处理AB(过硫酸氢钾复合盐和枯草芽孢杆菌均添加),采用16S rRNA基因高通量测序技术,分析了不同环境改良剂使用对河蟹肠道微生物群落的组成、结构和功能的影响。结果表明,环境改良剂处理时间延长对河蟹养殖水体总氮(TN)、总磷(TP)和铵态氮(NH_(4)^(+)-N)浓度及化学需氧量(COD_(Mn))的影响显著(P<0.05),TN、TP浓度及COD_(Mn)均表现为先升高后降低趋势,NH_(4)^(+)-N浓度表现为先平缓变化后升高的趋势,其中处理AB的4个水质指标值均显著低于对照组。虽然施用的不同环境改良剂对4种水质指标值未产生显著影响(P>0.05),但显著影响肠道细菌群落的组成,主要体现在厚壁菌门、变形菌门和拟杆菌门相对丰度变化上,以及枸橼酸杆菌属(Citrobacter)和Clostridium_sensu_stricto_1等特定菌属丰度的差异。群落多样性分析显示,施用时间对群落多样性具有显著影响(P<0.05),尤其在Chao1指数和PD指数上,而不同环境改良剂对群落多样性没有显著影响(P>0.05)。群落结构分析表明,施用时间和环境改良剂的交互作用对群落结构具有显著影响(P<0.05),但处理AB在维持群落稳定性方面效果较弱。功能预测分析结果显示,环境改良剂的使用显著影响肠道微生物群落功能,尤其在氨基酸代谢、脂质代谢、次级代谢产物合成和信号传导方面,且随着使用时间的延长,功能差异逐渐增大。因此,环境改良剂可通过改善养殖环境调节肠道微生物群落的组成、结构及功能,进而可能改善河蟹的免疫与代谢健康,本研究结果可为水产养殖环境优化提供理论依据。 展开更多
关键词 环境改良剂 河蟹养殖 肠道细菌 功能预测
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花生饼和蚯蚓粪配施对烤烟产量、品质及土壤特性的影响
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作者 程培军 徐敏 +11 位作者 李亮 索炎炎 郝浩浩 张翔 唐培培 王迅 郭传滨 许晓敬 徐凤丹 吴俊林 陈中尚 苗森 《河南农业科学》 北大核心 2026年第2期70-79,共10页
为了探讨花生饼和蚯蚓粪配施对烤烟产量、品质及土壤特性的影响,明确改良烟田土壤和提高烤烟产量、品质适宜的施肥模式,设置4个花生饼水平(0、300、600、900 kg/hm^(2),依次编号P_(0)、P_(300)、P_(600)、P_(900))、3个蚯蚓粪水平(0、50... 为了探讨花生饼和蚯蚓粪配施对烤烟产量、品质及土壤特性的影响,明确改良烟田土壤和提高烤烟产量、品质适宜的施肥模式,设置4个花生饼水平(0、300、600、900 kg/hm^(2),依次编号P_(0)、P_(300)、P_(600)、P_(900))、3个蚯蚓粪水平(0、500、1000 kg/hm^(2),依次编号V_(0)、V_(500)、V_(1000)),共12个处理开展大田试验,在采收结束时测定土壤微生物数量、酶活性和养分含量,烤烟经济性状、化学成分和外观质量。结果表明,蚯蚓粪相同用量下,在花生饼用量为0~900 kg/hm^(2)时,随着花生饼施用量增加,土壤细菌、真菌和放线菌数量,土壤蔗糖酶、脲酶、蛋白酶和酸性磷酸酶活性,土壤碱解氮、有效磷和速效钾含量显著增加,施花生饼较不施花生饼分别平均显著提高了26.0%、32.8%、24.7%,32.2%、75.8%、50.6%、69.2%,17.6%、13.1%、19.1%。花生饼相同用量下,随蚯蚓粪施用量增加,土壤细菌、真菌和放线菌数量,土壤蔗糖酶、脲酶、蛋白酶和酸性磷酸酶活性,土壤碱解氮、有效磷和速效钾含量亦呈现增加趋势,施蚯蚓粪较不施蚯蚓粪分别平均显著提高了14.9%、10.3%、16.9%,7.1%、14.1%、36.0%、39.5%,7.6%、6.4%、6.2%。施花生饼和蚯蚓粪均能改善烤烟化学成分和外观质量,提高烤烟产量,但施用效果受蚯蚓粪施用量的影响。在不施蚯蚓粪(V_(0))条件下,施花生饼较不施花生饼烤烟平均增产15.8%;在施蚯蚓粪(V_(500)、V_(1000))条件下,施花生饼较不施花生饼烤烟平均增产为22.2%。综上,施花生饼和蚯蚓粪均能提高土壤微生物数量、酶活性和养分含量,改善烤烟化学成分和外观质量,提高烤烟产量,以蚯蚓粪1000 kg/hm^(2)+花生饼600 kg/hm^(2)配施效果最佳。 展开更多
关键词 烤烟 花生饼 蚯蚓粪 产量 品质 土壤改良
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不同商用钝化剂对镉-汞复合污染麦田的修复效应及根际微生物群落影响
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作者 张欢欢 张心明 +2 位作者 彭俊伟 董元华 李建刚 《中国环境科学》 北大核心 2026年第1期269-278,共10页
选取6种无机商用钝化剂宁粮(NL)、天象(TX)、隆昌(LC)、沃农(WN)、格丰(GF)、百金惠(BJH),结合16S rRNA基因高通量测序技术进行根际微生物组学分析,探究了施用土壤钝化剂对镉、汞复合污染土壤中,小麦各部位镉汞积累转运特征及根际细菌... 选取6种无机商用钝化剂宁粮(NL)、天象(TX)、隆昌(LC)、沃农(WN)、格丰(GF)、百金惠(BJH),结合16S rRNA基因高通量测序技术进行根际微生物组学分析,探究了施用土壤钝化剂对镉、汞复合污染土壤中,小麦各部位镉汞积累转运特征及根际细菌群落结构的影响.田间试验结果表明,钝化剂处理使小麦籽粒镉生物富集系数(BCF_(Cd))降低20.93%~64.87%,汞生物富集系数(BCF_(Hg))下降13.44%~34.66%,土壤有效镉占比减少25.06%~39.58%.其中LC与WN处理对土壤镉固定及小麦镉转运抑制效果最优,且WN处理同步显著降低小麦汞转运.微生物分析显示,钝化剂通过富集耐镉菌群(如变形菌门、芽单胞菌门),改变根际细菌群落结构,进一步促进土壤重金属生物有效性下降.PICRUSt2功能预测表明,根际细菌群落通过富集氨基酸代谢与碳水化合物代谢,协同缓解重金属毒性.综上所述,钝化剂可直接降低土壤中重金属生物有效性,并间接富集根际耐受重金属菌群,协同阻控小麦中镉、汞的富集. 展开更多
关键词 复合污染 土壤钝化剂 根际微生物
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有机改良剂施用对风沙土胞外酶活性和土壤肥力的影响
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作者 刘天鸿 徐万里 +4 位作者 汤水荣 邬磊 孟磊 徐明岗 张文菊 《环境科学》 北大核心 2026年第1期592-599,共8页
为探究不同有机改良剂施用对风沙土理化性质和胞外酶活性的长期影响,于2010年在新疆维吾尔自治区和田县灌耕风沙土区设置:不施用有机改良剂(CK)、施用1%生物质炭(22.5 t·hm^(-2),B1)、10%生物质炭(225.0 t·hm^(-2),B2)和1%有... 为探究不同有机改良剂施用对风沙土理化性质和胞外酶活性的长期影响,于2010年在新疆维吾尔自治区和田县灌耕风沙土区设置:不施用有机改良剂(CK)、施用1%生物质炭(22.5 t·hm^(-2),B1)、10%生物质炭(225.0 t·hm^(-2),B2)和1%有机肥(22.5t·hm^(-2),M)这4个处理,试验期间种植春玉米,化学肥料施用量和农艺措施与当地农田管理模式保持一致. 2023年采集0~20 cm土壤样品,测定风沙土理化性质和胞外酶活性.有机改良剂有效改善风沙土理化性质,其中有机肥处理提升效果最佳.与CK相比,有机肥处理显著提高风沙土有机碳(43%,P<0.05)、全氮(55%,P<0.05)、田间持水量(14%,P<0.05)和微生物生物量碳(154%,P<0.05).有机肥施用通过提供可利用碳和有效态氮,缓解微生物碳限制并平衡氮磷养分需求.生物质炭由于高碳氮比和氮的吸附作用,加剧微生物的氮限制.基于现有观测结果,有机肥施用改善风沙土理化性质、酶活性和微生物养分平衡.生物质炭施用需结合氮素施用,避免氮限制.综上,通过阐明有机改良剂调节土壤酶活性和微生物养分限制的机制,丰富风沙土改良理论,可为提高风沙土土壤肥力和农业可持续发展提供科学依据和技术支持. 展开更多
关键词 风沙土 有机改良剂 胞外酶活性 微生物养分限制 土壤肥力
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药物临床试验“修正案”伦理审查的管理与实践
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作者 李星颐 陈仲林 +2 位作者 瞿幸池 冯钰 韩慧慧 《中国医学伦理学》 北大核心 2026年第1期58-63,共6页
近年来,随着加快药品研发的现实需求,以及试验设计的不断创新,临床试验进行期间的方案修订逐渐增多,且变更内容趋于灵活复杂,从而提升了修正案伦理审查的难度。在这一背景下,如何充分发挥伦理委员会的作用和职责,有效控制临床试验风险... 近年来,随着加快药品研发的现实需求,以及试验设计的不断创新,临床试验进行期间的方案修订逐渐增多,且变更内容趋于灵活复杂,从而提升了修正案伦理审查的难度。在这一背景下,如何充分发挥伦理委员会的作用和职责,有效控制临床试验风险并保障受试者安全有着重要意义。通过分析近年方案修订的发展趋势,梳理中国法规和指导原则中对方案修订的要求,结合实际工作中修正案伦理审查的难点,最终提出针对性的对策与建议,包括与科学性审查联动、强化形式审查、根据批准通知书调整审查范围、采用适当的审查方式,以期为药物临床试验修正案伦理审查的管理提供参考与借鉴。 展开更多
关键词 药物临床试验 伦理审查 修正案
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复合肥配施土壤改良剂对玉米生长和滨海盐碱地的影响
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作者 陈悦 薛志忠 +3 位作者 冯薇 孟然 吴哲 鲁雪林 《浙江农业科学》 2026年第1期211-215,共5页
为了改良河北省滨海盐碱地土壤理化特性并提升其耕地质量,本研究以河北省唐山市滨海盐碱地为研究对象,通过田间试验探究复合肥配施不同土壤改良剂对玉米生长及盐碱地改良效果的影响。试验设置7个处理,包含对照CK和6种不同用量的土壤改... 为了改良河北省滨海盐碱地土壤理化特性并提升其耕地质量,本研究以河北省唐山市滨海盐碱地为研究对象,通过田间试验探究复合肥配施不同土壤改良剂对玉米生长及盐碱地改良效果的影响。试验设置7个处理,包含对照CK和6种不同用量的土壤改良剂处理,分析了玉米农艺性状、土壤含盐量、pH值及土壤养分含量的变化。结果表明,复合肥配施土壤改良剂能够有效降低土壤含盐量、调节pH值、提高土壤养分含量,并促进玉米生长和增产。不同土壤改良剂的效果不尽相同,其中常规复合肥600 kg·hm^(-2)配施良田葆375 kg·hm^(-2)的综合效果最佳,适合在河北省滨海盐碱地推广应用。本研究为盐碱地改良和农业可持续发展提供了科学依据和实践参考。 展开更多
关键词 土壤改良剂 滨海盐碱地 玉米 土壤养分
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Newly Approved National Standards of P.R.China(No.29,2025)
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《China Standardization》 2026年第1期I0006-I0054,共49页
State Administration for Market Regulation and National Standardization Administration of China have approved the following 758 voluntary national standards and 6 voluntary national standards with amendment.
关键词 amendment standardization administration national standards voluntary national standards
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土壤改良剂在沙化草地治理中的应用研究
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作者 朱习雯 王英 +2 位作者 赵洁 巴音查汗 王采娥 《中国农机装备》 2026年第2期92-94,共3页
为提高沙化草地治理效果、加强生态修复能力、促进草地生态系统可持续发展,对土壤改良剂在沙化草地治理中的应用进行研究,具有十分重要的意义和应用价值。从土壤改良剂的作用机理出发,系统探讨了土壤改良剂在沙化草地治理中的应用效果,... 为提高沙化草地治理效果、加强生态修复能力、促进草地生态系统可持续发展,对土壤改良剂在沙化草地治理中的应用进行研究,具有十分重要的意义和应用价值。从土壤改良剂的作用机理出发,系统探讨了土壤改良剂在沙化草地治理中的应用效果,并结合牧草生产和饲料开发,为沙化草地的生态恢复和可持续利用提供理论依据和实践参考。 展开更多
关键词 土壤改良剂 沙化草地治理 应用研究
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