Coking wastewater,characterized by high biological toxicity,poses significant challenges for traditional biological treatment methods.This study developed a novel in-situ immobilized photocatalytic-algae-bacteria cons...Coking wastewater,characterized by high biological toxicity,poses significant challenges for traditional biological treatment methods.This study developed a novel in-situ immobilized photocatalytic-algae-bacteria consortia(P-ABC)system using a polyether polyurethane sponge as a carrier,aiming to enhance biological treatment efficiency for actual coking wastewater.Results showed a 16.8%increase in algal density(up to 1.51×10^(5) cells/mL)in the P-ABC system compared to non-coupled controls,with significantly improved microbial metabolic activity,confirming the carrier's exceptional biocompatibility.Compared to standalone algae-bacteria consortia systems,the P-ABC system achieved higher removal efficiencies for chemical oxygen demand(COD_(Cr),19.8%),total organic carbon(TOC,21.2%),and total nitrogen(TN,30.4%).These findings validate the system's potential for improving stable and efficient treatment of industrial wastewater.Furthermore,this study offers insights into bio-enhanced treatment technologies and provides a reference pathway for integrating advanced oxidation and biological processes.展开更多
Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the p...Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the process of ammonia nitrogen removal ,related data between ammonia removal and five factors was received through multi-factor orthogonal test,and established relations model between the five factor and nitrogen removal. The results show that five-factors had significant effect on AR,and the best combinations for removing AR was temperature 30 ℃,pH=7.0,light intensity 6 000 lux,dissolved oxygen 5.0 mg/L and the fill rate 10%. According to the experimental data,equation model was proposed and coefficient of determination R2 =0.864 8,P<0.05. Samples T-test was done between the model predictions and the actual measured values.Test results showed that the significant difference of overall mean value sig. (2-tailed) was 0.978 (P>0.05),it Shows that had no significant difference between model predictions and the actual measured value,and model had a high degree of fitting.展开更多
Microalgae can effectively degrade polycyclic aromatic hydrocarbons (PAHs) in water.However,the remediation mechanism of microalgae in PAH-contaminated soil remains unclear.In this study,the growth-promoting effects o...Microalgae can effectively degrade polycyclic aromatic hydrocarbons (PAHs) in water.However,the remediation mechanism of microalgae in PAH-contaminated soil remains unclear.In this study,the growth-promoting effects of wheat by Chlorella vulgaris in PAH-contaminated soil were studied.Structural changes in the rhizosphere bacterial community and the bacterial metabolism were further explored.It revealed that the addition of C.vulgaris promoted wheat’s dry weight and height by 10.22% and 122.15%,respectively.One explanation was the degradation and transformation of PAHs by C.vulgaris,which relieved the inhibitory effect on wheat growth.Compared with the blank control group,C.vulgaris addition enhanced the degradation efficiencies of phenanthrene (Phe) and pyrene (Pry) by 4.81% and 8.34%,respectively (with the initial concentrations in soil of 1.03×10^(4) and 2.21×10^(4)μg/g,respectively).The binding state of Phe and Pyr changed to a free state,which facilitated microbial degradation.The Phe and Pyr contents in wheat decreased by 22.23% and 18.54%,respectively.The presence of C.vulgaris increased the abundance of Sphingosinomonas bacteria capable of degrading PAHs by 95.24%.Enzyme activities related to the transport,oxidation,and dehydrogenation of PAHs in the bacterial community also increased.This study demonstrated C.vulgaris’multiple pathways for remediating PAH-polluted soil,including PAH degradation,nutrient and hormone release,and bacterial community adjustment.In conclusion,C.vulgaris addition enhanced the algae-bacteria symbiosis,which was of great significance for the removal of PAHs from the soil and the promotion of plant growth.展开更多
Macroalgae farming not only holds significant economic value but also contributes substantially to carbon sequestration,and therefore has gained intensified attention globally under climate change scenarios.However,it...Macroalgae farming not only holds significant economic value but also contributes substantially to carbon sequestration,and therefore has gained intensified attention globally under climate change scenarios.However,its sustainability is increasingly threatened by anthropogenic and environmental changes.The health and resilience of macroalgae are intrinsically linked to their associated microbiomes,offering an untapped opportunity to enhance macroalgal farming through microbiome manipulation.In this review,we have summarized the current understanding of macroalgal microbiomes,highlighting critically underexplored microbial components,such as overlooked taxa,host specificity,and the environmental factors influencing microbiome composition,which hinder the development of effective microbiome engineering strategies.We critically evaluate existing microbiome manipulation approaches and their applications in enhancing macroalgal growth,resilience,carbon fixation,and biomass yield and assess their potential for improving macroalgal carbon sequestration.Finally,we propose a holistic framework that integrates multi-omics and metabolic modeling,microbial functional and environmental compatibility,high-throughput rapid isolation,and in vivo validation to bridge critical knowledge gaps and unlock the full potential of macroalgal microbiome engineering for sustainable,large-scale macroalgal farming.展开更多
A comprehensive kinetic model called anaerobic digestion bacteria algae(ADBA)was developed to describe and predict the complex algae-bacterial system in anaerobic digestion(AD)wastew-ater under mixotrophic growth cond...A comprehensive kinetic model called anaerobic digestion bacteria algae(ADBA)was developed to describe and predict the complex algae-bacterial system in anaerobic digestion(AD)wastew-ater under mixotrophic growth conditions.The model was calibrated and validated using the experimental growth data from cultivating the algae(Chlorella vulgaris CA1)with its indigenous bacteria in Blue Green 11(BG-11)media and different combinations of sterilized,diluted,and raw AD effluent.Key parameters were obtained,including the distinct maximum growth rate of algae on CO_(2)(μ_(a,CO_(2)),1.23 per day)and organic carbon(μ_(a,OC),3.30 per day),the maximum growth rate of bacteria(μ_(b),1.20 per day),along with two noble parameters,i.e.,the algae-bacteria in-teraction exponent(n,0.03)and the growth inhibition coefficient(a_(e)=30000 mg/L per AU)due to effluent turbidity.The model showed a good fit with an average R^(2)=0.90 in all cases adjusted with 25 kinetic parameters.This was the first model capable of predicting algal and bac-terial growth in AD effluent with their competitive interactions,assuming shifting growth modes of algae on organic and inorganic carbon under light.It could also predict the removal rate of substrate and nutrients from effluent,light inhibition due to biomass shading and effluent turbid-ity,mass transfer rate of O_(2) and CO_(2)from gas phase to liquid phase,and pH-driven equilibrium between dissolved inorganic carbon components(CO_(2),HCO_(3)^(-),and CO_(3)^(2-)).Algal growth in the strongly buffered AD effluent resulted in odor removal,turbidity reduction,and the removal of∼80%of total ammonium-nitrogen and 90%of organic carbon.In addition to process parame-ter prediction,this study offered a practical solution to wastewater treatment,air pollution,and nutrient recycling,ensuring a holistic and practical approach to ecological balance.展开更多
The existence of continually increasing concentrations of antibiotics in the environment is a serious potential hazard due to their toxicity and persistence.Unfortunately,conventional treatment techniques,such as thos...The existence of continually increasing concentrations of antibiotics in the environment is a serious potential hazard due to their toxicity and persistence.Unfortunately,conventional treatment techniques,such as those utilized in wastewater treatment plants,are not efficient for the treatment of wastewater containing antibiotic.Recently,algae-based technologies have been found to be a sustainable and promising technique for antibiotic removal.Therefore,this review aims to provide a critical summary of algae-based technologies and their important role in antibiotic wastewater treatment.Algal removal mechanisms including bioadsorption,bioaccumulation,and biodegradation are discussed in detail,with using algae-bacteria consortia for antibiotic treatment,integration of algae with other microorganisms(fungi and multiple algal species),hybrid algae-based treatment and constructed wetlands,and the factors affecting algal antibiotic degradation comprehensively described and assessed.In addition,the use of algae as a precursor for the production of biochar is highlighted,along with the modification of biochar with other materials to improve its antibiotic removal capacity and hybrid algae-based treatment with advanced oxidation processes.Furthermore,recent novel approaches for enhancing antibiotic removal,such as the use of genetic engineering to enhance the antibiotic degradation capacity of algae and the integration of algal antibiotic removal with bioelectrochemical systems are discussed.Finally,some based on the critical review,key future research perspectives are proposed.Overall,this review systematically presents the current progress in algae-mediated antibiotic removal technologies,providing some novel insights for improved alleviation of antibiotic pollution in aquatic environments。展开更多
基金supported by the National Natural Science Foundation of China(No.22076113)Shaanxi Province Key R&D Program Project(No.2020NY-235)。
文摘Coking wastewater,characterized by high biological toxicity,poses significant challenges for traditional biological treatment methods.This study developed a novel in-situ immobilized photocatalytic-algae-bacteria consortia(P-ABC)system using a polyether polyurethane sponge as a carrier,aiming to enhance biological treatment efficiency for actual coking wastewater.Results showed a 16.8%increase in algal density(up to 1.51×10^(5) cells/mL)in the P-ABC system compared to non-coupled controls,with significantly improved microbial metabolic activity,confirming the carrier's exceptional biocompatibility.Compared to standalone algae-bacteria consortia systems,the P-ABC system achieved higher removal efficiencies for chemical oxygen demand(COD_(Cr),19.8%),total organic carbon(TOC,21.2%),and total nitrogen(TN,30.4%).These findings validate the system's potential for improving stable and efficient treatment of industrial wastewater.Furthermore,this study offers insights into bio-enhanced treatment technologies and provides a reference pathway for integrating advanced oxidation and biological processes.
基金Supported by the National Natural Science Foundation of China(No.30972260)~~
文摘Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the process of ammonia nitrogen removal ,related data between ammonia removal and five factors was received through multi-factor orthogonal test,and established relations model between the five factor and nitrogen removal. The results show that five-factors had significant effect on AR,and the best combinations for removing AR was temperature 30 ℃,pH=7.0,light intensity 6 000 lux,dissolved oxygen 5.0 mg/L and the fill rate 10%. According to the experimental data,equation model was proposed and coefficient of determination R2 =0.864 8,P<0.05. Samples T-test was done between the model predictions and the actual measured values.Test results showed that the significant difference of overall mean value sig. (2-tailed) was 0.978 (P>0.05),it Shows that had no significant difference between model predictions and the actual measured value,and model had a high degree of fitting.
基金supported by the National Natural Science Foundation of China (Nos.U24A20613 and 52370043)China National Funds for Distinguished Young Scientists (No.51925803)。
文摘Microalgae can effectively degrade polycyclic aromatic hydrocarbons (PAHs) in water.However,the remediation mechanism of microalgae in PAH-contaminated soil remains unclear.In this study,the growth-promoting effects of wheat by Chlorella vulgaris in PAH-contaminated soil were studied.Structural changes in the rhizosphere bacterial community and the bacterial metabolism were further explored.It revealed that the addition of C.vulgaris promoted wheat’s dry weight and height by 10.22% and 122.15%,respectively.One explanation was the degradation and transformation of PAHs by C.vulgaris,which relieved the inhibitory effect on wheat growth.Compared with the blank control group,C.vulgaris addition enhanced the degradation efficiencies of phenanthrene (Phe) and pyrene (Pry) by 4.81% and 8.34%,respectively (with the initial concentrations in soil of 1.03×10^(4) and 2.21×10^(4)μg/g,respectively).The binding state of Phe and Pyr changed to a free state,which facilitated microbial degradation.The Phe and Pyr contents in wheat decreased by 22.23% and 18.54%,respectively.The presence of C.vulgaris increased the abundance of Sphingosinomonas bacteria capable of degrading PAHs by 95.24%.Enzyme activities related to the transport,oxidation,and dehydrogenation of PAHs in the bacterial community also increased.This study demonstrated C.vulgaris’multiple pathways for remediating PAH-polluted soil,including PAH degradation,nutrient and hormone release,and bacterial community adjustment.In conclusion,C.vulgaris addition enhanced the algae-bacteria symbiosis,which was of great significance for the removal of PAHs from the soil and the promotion of plant growth.
基金supported by the National Key Research and Development Program of China(2020YFA0608304,2020YFA0607603)the Natural Science Foundation of China(42176050,42276105,U1906216)+4 种基金the China Postdoctoral Science Foundation(2024M753347)the Shandong Province Postdoctoral Fund Project(SDBX2022030,SDCX-ZG-202400160)the Youth Innovation Promotion Association of CAS(2023220)the Taishan Scholar Foundation of Shandong Province(No.tsqn2024)the Ocean Negative Carbon Emissions(ONCE)Project.
文摘Macroalgae farming not only holds significant economic value but also contributes substantially to carbon sequestration,and therefore has gained intensified attention globally under climate change scenarios.However,its sustainability is increasingly threatened by anthropogenic and environmental changes.The health and resilience of macroalgae are intrinsically linked to their associated microbiomes,offering an untapped opportunity to enhance macroalgal farming through microbiome manipulation.In this review,we have summarized the current understanding of macroalgal microbiomes,highlighting critically underexplored microbial components,such as overlooked taxa,host specificity,and the environmental factors influencing microbiome composition,which hinder the development of effective microbiome engineering strategies.We critically evaluate existing microbiome manipulation approaches and their applications in enhancing macroalgal growth,resilience,carbon fixation,and biomass yield and assess their potential for improving macroalgal carbon sequestration.Finally,we propose a holistic framework that integrates multi-omics and metabolic modeling,microbial functional and environmental compatibility,high-throughput rapid isolation,and in vivo validation to bridge critical knowledge gaps and unlock the full potential of macroalgal microbiome engineering for sustainable,large-scale macroalgal farming.
基金supported by the Applied BioEnergy Research Program Internal Competitive Grant from the Agricultural Research Center at Washington State University,College of Agricultural,Human,and Natural Resource Sciences.
文摘A comprehensive kinetic model called anaerobic digestion bacteria algae(ADBA)was developed to describe and predict the complex algae-bacterial system in anaerobic digestion(AD)wastew-ater under mixotrophic growth conditions.The model was calibrated and validated using the experimental growth data from cultivating the algae(Chlorella vulgaris CA1)with its indigenous bacteria in Blue Green 11(BG-11)media and different combinations of sterilized,diluted,and raw AD effluent.Key parameters were obtained,including the distinct maximum growth rate of algae on CO_(2)(μ_(a,CO_(2)),1.23 per day)and organic carbon(μ_(a,OC),3.30 per day),the maximum growth rate of bacteria(μ_(b),1.20 per day),along with two noble parameters,i.e.,the algae-bacteria in-teraction exponent(n,0.03)and the growth inhibition coefficient(a_(e)=30000 mg/L per AU)due to effluent turbidity.The model showed a good fit with an average R^(2)=0.90 in all cases adjusted with 25 kinetic parameters.This was the first model capable of predicting algal and bac-terial growth in AD effluent with their competitive interactions,assuming shifting growth modes of algae on organic and inorganic carbon under light.It could also predict the removal rate of substrate and nutrients from effluent,light inhibition due to biomass shading and effluent turbid-ity,mass transfer rate of O_(2) and CO_(2)from gas phase to liquid phase,and pH-driven equilibrium between dissolved inorganic carbon components(CO_(2),HCO_(3)^(-),and CO_(3)^(2-)).Algal growth in the strongly buffered AD effluent resulted in odor removal,turbidity reduction,and the removal of∼80%of total ammonium-nitrogen and 90%of organic carbon.In addition to process parame-ter prediction,this study offered a practical solution to wastewater treatment,air pollution,and nutrient recycling,ensuring a holistic and practical approach to ecological balance.
基金the National Natural Science Foundation of China(No.52070057)the National Key Research and Development Program(No.2019YFC0408503)the National Natural Science Foundation of China(No.51961165104).
文摘The existence of continually increasing concentrations of antibiotics in the environment is a serious potential hazard due to their toxicity and persistence.Unfortunately,conventional treatment techniques,such as those utilized in wastewater treatment plants,are not efficient for the treatment of wastewater containing antibiotic.Recently,algae-based technologies have been found to be a sustainable and promising technique for antibiotic removal.Therefore,this review aims to provide a critical summary of algae-based technologies and their important role in antibiotic wastewater treatment.Algal removal mechanisms including bioadsorption,bioaccumulation,and biodegradation are discussed in detail,with using algae-bacteria consortia for antibiotic treatment,integration of algae with other microorganisms(fungi and multiple algal species),hybrid algae-based treatment and constructed wetlands,and the factors affecting algal antibiotic degradation comprehensively described and assessed.In addition,the use of algae as a precursor for the production of biochar is highlighted,along with the modification of biochar with other materials to improve its antibiotic removal capacity and hybrid algae-based treatment with advanced oxidation processes.Furthermore,recent novel approaches for enhancing antibiotic removal,such as the use of genetic engineering to enhance the antibiotic degradation capacity of algae and the integration of algal antibiotic removal with bioelectrochemical systems are discussed.Finally,some based on the critical review,key future research perspectives are proposed.Overall,this review systematically presents the current progress in algae-mediated antibiotic removal technologies,providing some novel insights for improved alleviation of antibiotic pollution in aquatic environments。
