Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflamm...Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflammatory activity of SA extracts by petroleum ether（PE）, ethyl acetate（EtOAc）, and H2O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF--α, and IL-6 production in RAW 264.7 cells（P0.01）, suggesting that this extract possessed potent anti--inflammatory activity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p--hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the Et -OAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti--inflammatory agent.展开更多
This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ra...This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.展开更多
Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a l...Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a lot relationships between CA125 level and liver cirrhosis,this case had a obvious increased CA125 level in the serum,hydrothorax and ascites,following by the liver cirrhosis in a short time,and then died of upper gastrointestinal bleeding.By now we guess that CA125 level could forecast the liver cirrhosis following by hepatic veno-occlusive,which will become the prognosis of the hepatic veno-occlusive.展开更多
Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(m...Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.展开更多
A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are ofte...A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.展开更多
Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal ag...Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.展开更多
基金Quality guarantee system of Chinese herbal medicines(Grant No.201507002)Key project of science and technology of Fujian province(Grant No.2014Y0053)youth project of Fujian Provincial Health and Family Planning Commission(Grant No.2015--1--77)
文摘Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflammatory activity of SA extracts by petroleum ether（PE）, ethyl acetate（EtOAc）, and H2O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF--α, and IL-6 production in RAW 264.7 cells（P0.01）, suggesting that this extract possessed potent anti--inflammatory activity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p--hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the Et -OAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti--inflammatory agent.
文摘This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.
文摘Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a lot relationships between CA125 level and liver cirrhosis,this case had a obvious increased CA125 level in the serum,hydrothorax and ascites,following by the liver cirrhosis in a short time,and then died of upper gastrointestinal bleeding.By now we guess that CA125 level could forecast the liver cirrhosis following by hepatic veno-occlusive,which will become the prognosis of the hepatic veno-occlusive.
基金The work was supported by the Natural Science Foundation Guidance Project of Liaoning(2018055455)the Young and Middle-aged Key Talent Training Program of Shenyang Pharmaceutical Universitythe Foundation of Education Department of Liaoning Province(2017LQN09).
文摘Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.
基金supported by Natural Science Foundation of Zhejiang Province[LY16C200003]Zhejiang Provincial Key Research and Development,Program[2020C02037].
文摘A traditional herb that is rich in active substances such as flavonoids, polyphenols and alkaloids, Sedum aizoon L . has antioxidant and preventive effects against chronic diseases. Circadian rhythm disorders are often accompanied by changes in intestinal flora, and flavonoids that can regulate intestinal flora may be an effective way to prevent and regulate circadian rhythm disorders. In this study, we established a humanized circadian rhythm mouse model to explore the regulatory mechanism of flavonoids from Sedum aizoon L .(FSAL) on circadian rhythm disorders in mice. The results after feeding FSAL for 4 weeks showed that FSAL improved the imbalance of intestinal microbial structure caused by circadian rhythm disorders, thus regulating alanine, aspartate and glutamate metabolism, histidine metabolism and other metabolites and substances related to metabolism. The analysis of liver transcriptome showed that FSAL significantly regulated retinol metabolism, peroxisome proliferator-activated receptors(PPARs) pathway, and hepatitis C pathway in mouse liver. Therefore, this study obtained the conclusion that FSAL can regulate intestinal flora, metabolites and liver genes to improve circadian rhythm disorders and maintain mouse health.
基金This research was kindly supported through funding from National Natural Science Foundation of China(31772030)Student Research and Innovation Program of Ningbo University(2021SRIP3607).
文摘Botrytis cinerea is a plant pathogen,which affects the quality of fruits.However,chemical antifungal agents are extremely harmful to the environment,hence it’s critical to create a natural plant-derived antifungal agent to replace chemical antifungal agents.The study explored the antifungal mechanisms of flavonoids from Sedum aizoon L.(FSAL)against B.cinerea.In the study,cell membrane served as an important breakthrough point.The minimum inhibitory concentration(MIC)of FSAL against B.cinerea was investigated.The propidium iodide(PI)staining observation of B.cinerea was explored.The activities of crucial enzymes involved in the metabolism were studied as well as the phospholipid and fatty acid changes.The expression of genes related to the membrane lipid metabolism was also measured.The results showed that FSAL had a certain inhibitory effect on B.cinerea and the MIC was 1.500 mg/mL.FSAL disrupted the cell membrane of B.cinerea,which decreased the contents of glutathione and proline at a low level as well as the membrane phospholipids.Compared with the control group,the activities of crucial enzymes were decreased,and the expression of key genes was reduced.It indicated that FSAL inhibited B.cinerea by disrupting its cell membrane,offering the possibility of a natural plant-derived antifungal agent for the preservation of fruits.
