Triphenylamine(TPA)-based aggregation-induced emission luminogens(TPA-AIEgens),a type of photoactive material utilizing the typical TPA moiety,has recently attracted increasing attention for the diagnostics and treatm...Triphenylamine(TPA)-based aggregation-induced emission luminogens(TPA-AIEgens),a type of photoactive material utilizing the typical TPA moiety,has recently attracted increasing attention for the diagnostics and treatment of tumors due to their remarkable chemo-physical performance in optoelectronic research.TPA-AIEgens are distinguished from other photoactive agents by their strong fluorescence,good sensitivity,high signal-to-noise ratio,resistance to photobleaching,and lack of high concentration or aggregation-caused fluoresce quenching effects.In this review,we summarize the current advancements and the biomedical progress of TPA-AIEgens in tumor theranostics.First,the design principles of TPAAIEgens photoactive agents as well as the advanced targeting strategies for nuclei,cell membranes,cell organelle and tumors were introduced,respectively.Next,the applications of TPA-AIEgens in tumor diagnosis and therapeutic techniques were reviewed.Last,the challenges and prospects of TPA-AIEgens for cancer therapy were performed.The given landscape of the TPA-AIEgens hereby is meaningful for the further design and utilization of the novel photoactive material,which could be beneficial for the development of clinic applications.展开更多
An AIEgen decorated porphyrin(TPETPyP)was easily obtained through a one-step reaction.The bulky TPE in TPETPyP greatly impeded the intermolecularπ-πstacking of the porphyrin core,which significantly suppressed aggre...An AIEgen decorated porphyrin(TPETPyP)was easily obtained through a one-step reaction.The bulky TPE in TPETPyP greatly impeded the intermolecularπ-πstacking of the porphyrin core,which significantly suppressed aggregation-caused quenching(ACQ)effect of TPETPyP in aqueous solution.The four pyridinium salts formed in TPETPyP also render the whole molecule water solubility,which eliminated its aggregation.TPETPyP exhibited 1O2 quantum yield as high as 0.85 in PBS.Moreover,it also showed high binding affinity to proteins,the major biotarget of 1O2.The high 1O2 quantum yield plus the great binding ability of TPETPyP toward proteins makes it a highly-efficient protein photocleaving agent.Protein electrophoresis experiments demonstrated that TPETPyP can photocleave BSA upon visible light irradiation,indicating that TPETPyP can act as a promising photosensitizer(PS)in PDT.The work here will provide a facile strategy to utilize AIEgens modified traditional PSs for photodynamic therapy(PDT).展开更多
In recent twenty years,aggregation-induced emission(AIE),due to its excellent application prospect,has aroused widespread interests.The development of novel and easy to make AIE luminogens(AIEgens)is an attractive sub...In recent twenty years,aggregation-induced emission(AIE),due to its excellent application prospect,has aroused widespread interests.The development of novel and easy to make AIE luminogens(AIEgens)is an attractive subject.For this purpose,it is very important to study the structure-property relationship of AIEgens.Because azine derivatives are easy to synthesis and some of them have nice AIE properties,herein,a series of azine derivatives(ADs)were employed as models to study the influence of different functional groups,electronic effects and structures on the AIE properties of azine derivatives.The AIE mechanism were studied by single crystal analysis,density functional theory(DFT)calculations and so on.The results indicated that the o-hydroxyl aryl substituted azine compounds could show good AIE properties.Meanwhile,the AIE properties of o-hydroxyl aryl substituted azine compounds were also influenced by the electronic effects of the aryl groups in the azine compounds.The o-hydroxyl groups could form intramolecular hydrogen bond with imine group,which play key role to restrict the intramolecular rotation of the aryl groups and act as base stone for the AIE process of this kind compounds.The HOMO-LUMO energy gaps of o-hydroxyl substituted azine are smaller than other homologous compounds,which is agree with the proposed AIE mechanism.Finally,thanks to the AIE properties,the o-hydroxy-substituted azines could be used as efficient Al^(3+)and Cu^(2+)fluorescent chemosensors in different conditions.In addition,test strips based on AD10 has been prepared,which can conveniently detect Cu^(2+)in industrial wastewater.This research supplied a way for the design of novel easy to make AIEgens through simple azine derivatives.展开更多
Aggregation-induced emission luminogens(AIEgens) have been used in biomacromolecules detection.Herein,TPE-dC and TPE-dU acted as the nucleoside-based AIEgens sensors in the first case,which can be used to detect ctDNA...Aggregation-induced emission luminogens(AIEgens) have been used in biomacromolecules detection.Herein,TPE-dC and TPE-dU acted as the nucleoside-based AIEgens sensors in the first case,which can be used to detect ctDNA and rRNA in vitro and light up the nucleus in vivo depending on the intermolecular binding affinity.This AIE process enables the quantitative analysis or visualization of nucleic acids in solution or gels state,respectively.Furthermore,confocal laser scanning microscopy(CLSM) images of L929 cells stained with TPE-dC or TPE-dU clearly shows that nucleoside-based AIEgens bio-probes can pass the cell membranes to reach the cell nucleus,without cytotoxicity at the imaging condition(incubation time> 12 h,and 10 μmol/L of concentration).Since the nucleus is rich in DNA/RNA,fluorescence turn-on mode has a great potential in nucleus imaging and clinical diagnosis.展开更多
Supramolecular architectures exhibiting cascade energy transfer characteristics represent pivotal model systems for advancing biomimetic light-harvesting systems(LHS)that emulate the natural photosynthesis.To now,the ...Supramolecular architectures exhibiting cascade energy transfer characteristics represent pivotal model systems for advancing biomimetic light-harvesting systems(LHS)that emulate the natural photosynthesis.To now,the engineering of aqueous-phase artificial LHS with optimized energy transfer cascades is still a challenge.In this study,we designed and synthesized two tetraphenylethylene(TPE)-based macrocyclic compounds(namely TPE-1 and TPE-2)with different cavity sizes as supramolecular scaffolds to study their energy transfer behaviors.As a control model,a linear molecule TPE-3 was also prepared.The bigger-cavity macrocycle TPE-1 can emit green fluorescence and self-assemble into nanospherical structures in aqueous media,acting as an energy donor.Through self-assembly with eosin Y(EY)and a red-emitting fluorophore(TPE-Se),a sequential Forster resonance energy transfer(FRET)cascade:TPE-1→EY→TPE-Se was achieved thanks to their excellent spectral overlap and proximity between the donor and acceptors.The optimized ternary system(TPE-1/EY/TPE-Se)with a ratio of 1000:90:60 afforded a high energy transfer efficiency(Φ_(ET))of 95%.Then,the artificial LHS platform catalyzed the oxidative coupling of benzylamines with 93%yield in aqueous media.Moreover,the system demonstrated broad catalytic utility oxidation reactions,the good conversion of methylthiobenzyl ester to methylbenzene sulfoxide and the aerobic cross-dehydrogenation coupling reaction of N-phenyltetrahydroisoquinoline with indole.These results robustly demonstrate the promising potential of this artificial LHS in the field of aqueous photocatalysis.展开更多
Theranostics,possessing both imaging and therapeutic functions,has emerged as a promising strategy for the efficient treatment of cancer.Aggregation-induced emission luminogen(AIEgen)functionalized inorganic-organic h...Theranostics,possessing both imaging and therapeutic functions,has emerged as a promising strategy for the efficient treatment of cancer.Aggregation-induced emission luminogen(AIEgen)functionalized inorganic-organic hybrid nanomaterials have attracted great interest in biomedicine due to their distinct advantages,such as good biocompatibility,excellent fluorescence properties,facile functionalization,etc.展开更多
Inorganic bioactive nanomaterials play a significant role in biomedical fields due to their excellent biocompatibility.AIEgen-functionalised mesoporous bioactive glass(FMBG)nanoparticles with sizes of 60-100 nm are pr...Inorganic bioactive nanomaterials play a significant role in biomedical fields due to their excellent biocompatibility.AIEgen-functionalised mesoporous bioactive glass(FMBG)nanoparticles with sizes of 60-100 nm are prepared by post-grafting tetraphenylethene onto bioactive glass.The obtained nanoparticles emit strong blue light at 482 nm upon UV irradiation,showing potential application in cell imaging.Furthermore,the FMBG,with a high DOX loading capacity of 250.74μg mg^(-1)and good degradability in an acid environment.展开更多
Aggregation-induced emission luminogen(AIEgen)-functionalised mesoporous silica nanoparticles were synthesized by post-grafting two types of tetraphenylethene derivatives on mesoporous materials.The fluorescent chemic...Aggregation-induced emission luminogen(AIEgen)-functionalised mesoporous silica nanoparticles were synthesized by post-grafting two types of tetraphenylethene derivatives on mesoporous materials.The fluorescent chemical sensors,denoted as FMSN-1 and FMSN-2,emitted strong blue fluorescence under UV irradiation.Both of them exhibited quick and sensitive responses to 2,4,6-trinitrophenol(PA)and 2,4-dinitrophenol(2,4-DNP)via a fluorescence quenching process.In particular,the obtained materials also showed remarkable sensing ability for the antibiotics furazolidone(FZD)and nitrofurazone(NF)with low detection limits.The highly efficient sensing abilities of FMSNs for PA,2,4-DNP,FZD,and NF can be attributed to fluorescence resonance energy transfer(FRET)from AIEgens to analytes.These results demonstrate the potential value of AIEgen-functionalised mesoporous materials in environmental protection.展开更多
Aggregation-induced emission luminogens(AIEgens),tetraphenylethene,were post grafted onto mesoporous silica nanoparticles to form AIEgen-functionalised mesoporous materials.The obtained blue-emitting materials can ser...Aggregation-induced emission luminogens(AIEgens),tetraphenylethene,were post grafted onto mesoporous silica nanoparticles to form AIEgen-functionalised mesoporous materials.The obtained blue-emitting materials can serve as a fluorescence resonance energy transfer(FRET)donor for monitoring the release process of doxorubicin hydrochloride(DOX)as an acceptor by the change of the fluorescent signal,showing potential application in imaging-guided therapy.展开更多
Ruthenium polypyridyl complexes are known for their excellent photophysical properties and rich electrochemical behavior.Thus,developing complexes with multifunctional behavior showing potential applications in multip...Ruthenium polypyridyl complexes are known for their excellent photophysical properties and rich electrochemical behavior.Thus,developing complexes with multifunctional behavior showing potential applications in multiple fields is of great importance.Herein,we report a new aggregation-induced emission(AIE)active bis-heteroleptic ruthenium(Ⅱ)complex(Ru-4)of 4,7-diamino 1,10-phenanthroline along with a series of other bis-heteroleptic Ru(Ⅱ)polypyridyl complexes(Ru-1,Ru-2 and Ru-3)containing 4,7-disubstituted 1,10-phenanthroline and two homoleptic complexes(Ru-5 and Ru-6)of 2,2’-bipyridine and 1,10-phenanthroline.展开更多
Apoptotic vesicles(ApoVs)are membrane structures formed during cell apoptosis and play crucial roles in homeostasis maintenance,signal transduction,and immune regulation.Importantly,ApoVs inherit the properties and co...Apoptotic vesicles(ApoVs)are membrane structures formed during cell apoptosis and play crucial roles in homeostasis maintenance,signal transduction,and immune regulation.Importantly,ApoVs inherit the properties and contents of parental cells that show great potential in the diagnosis and treatment of diseases.Monitoring the formation process of ApoVs(such as quantity,morphological changes,release rules,etc.)can reveal the regulatory mechanism of apoptosis,and is also helpful for optimizing the preparation and application of ApoVs.However,due to the limitations of existing technologies,the formation processes of ApoVs have been challenging to precisely and entirely capture.Herein,we subtly constructed a versatile AIEgen(ADTP)that could induce ApoVs production and in situ monitor the formation process,and it was successfully applied to explore the formation mechanism of ApoVs.ADTP specifically targeted the plasma membrane,and it could effectively induce apoptosis under laser irradiation,so it was able to dynamically monitor the entire formation process of ApoVs and had validated ApoVs formation from membrane protrusions(including filopodia,tunneling nanotubes,and retraction fibers).Further investigation revealed that ApoVs derived from membrane protrusions with different components exhibited significant heterogeneity.Additionally,the nearinfrared emission characteristic of ADTP was compatible with the stimulated emission depletion(STED)microscopy equipped with a 775nmdepletion laser,enabling high-resolution visualization of detailed dynamic changes inmembrane protrusions during ApoVs formation.This work provided powerful tools for tracking the entire ApoVs formation process and also offered crucial scientific evidence for revealing the ApoVs formation mechanism.展开更多
Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious...Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.展开更多
Luminogens with aggregation-induced emission(AIEgens)have a wide range of biomedical applications in bioimaging,photodynamic anticancer,antibacterial therapy,and other fields,owing to their unique photophysical proper...Luminogens with aggregation-induced emission(AIEgens)have a wide range of biomedical applications in bioimaging,photodynamic anticancer,antibacterial therapy,and other fields,owing to their unique photophysical properties.The precise structural design and modification of AIE molecules have aroused great interest in the past years.As peptides-AIE hybrid materials,peptide-based AIEgens generally have better solubility,biocompatibility,and lower systemic toxicity.The functional diversity,modularity,and portability of peptides provide more possibilities for the intelligent structure and functional design of AIEgens.This review summarizes the recent research progress of peptide-based AIEgens nanomaterials,from molecular design,stimuli responsiveness to biomedical application,focusing on the advantages of peptides and AIE molecules as conjugates.Finally,a summary of the challenges and opportunities of peptide-based AIEgens nanomaterials for future clinical biomedical applications is presented.展开更多
Molecular interactions are crucial in diverse fields of protein folding,material science,nanotechnology,and life origins.Although mounting experimental research controls luminescent behavior by adjusting molecular int...Molecular interactions are crucial in diverse fields of protein folding,material science,nanotechnology,and life origins.Although mounting experimental research controls luminescent behavior by adjusting molecular interactions in light-emitting materials,it remains elusive to correlate microscopic molecular interactions with macroscopic luminescent behavior directly.Here,we synthesized three red luminogens with subtle structural variation and investigated the influence of molecular interactions on their luminescent behavior in solution and aggregate states.Our results indicate that strongπ-πand D-A interactions in both dilute solution(between luminogen and solvent molecules)and aggregate(between luminogens)states cause the redshift in emission,while weak interactions(e.g.,Van der Waals,C–H…π,and C–H…F interactions)enhance the quantum yield.This work provides a thoughtful investigation into the complicated influence of various molecular interactions on luminescent behavior.展开更多
Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified ...Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.展开更多
A new class of near-infrared(NIR)fluorescent organoboron AIEgens was successfully developed for latent fingerprints(LFPs)imaging.They exhibit real-time and in situ high-resolution imaging performance at 1-3 levels of ...A new class of near-infrared(NIR)fluorescent organoboron AIEgens was successfully developed for latent fingerprints(LFPs)imaging.They exhibit real-time and in situ high-resolution imaging performance at 1-3 levels of LFPs by spraying method.In addition,we systematically elucidate the fingerprint imaging mechanism of these AIEgens.Significantly,the excellent level 3 structural imaging capabilities enable the application of them for analyzing incomplete LFPs and identifying individuals in different scenarios.展开更多
Aggregation-induced emission-based luminogens(AIEgens)have aroused enormous interest due to their unique high fluorescence in a condensed state.To further explore their potential applications,such as chemical monitori...Aggregation-induced emission-based luminogens(AIEgens)have aroused enormous interest due to their unique high fluorescence in a condensed state.To further explore their potential applications,such as chemical monitoring,immobilization of AIE molecules has been widely studied with a variety of supports.Crystalline porous materials,such as metal-organic frameworks,covalent organic frameworks,hydrogen-bonded organic framework,and organic cages,demonstrate well-controlled structures,large surface areas,and promising stabilities,thus providing a perfect platform for AIE agents loading.Outstanding chemical sensing performances are achieved based on these AIE-active crystalline porous materials,such as high sensitivity,short response time,selective identification,and high recyclability,which provide a new alternative to readily detect various hazardous molecules.Furthermore,precise structures of AIEgen-based crystalline porous materials offer an easy way to investigate detection mechanisms.This mini-review will provide a brief overview of AIEgen-based crystalline porous materials for detection and then address how to improve sensing performances remarkably.展开更多
Activity-based approaches for designing AIEgens possess prominent advantages including high selectivity,sensitivity,and signal-to-noise ratio,and they have received more attention in recent years.Excellent activatable...Activity-based approaches for designing AIEgens possess prominent advantages including high selectivity,sensitivity,and signal-to-noise ratio,and they have received more attention in recent years.Excellent activatable AIE probes have been reported for detecting toxic substances,imaging intracellular active molecules/biomolecules,as well as monitoring the activity of overexpressed enzymes in cancers.Moreover,the majority of activatable theranostic AIEgens can be specifically triggered in cancer cells and can kill these cells under light irradiation,while they have no distinct effect on normal cells,demonstrating satisfactory therapeutic selectivity that is superior to that of traditional chemotherapy.Thus,in this review,we systematically summarized the development of activatable AIE bioprobes in recent years from molecular design principles to biological applications.The challenges of activatable AIE probes and the corresponding solutions are described.We hope that the information provided in this review will facilitate the design of more activatable AIE probes to promote practical application of corresponding AIEgens.展开更多
基金funded by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.820LH027)the Hainan Provincial Natural Science Foundation of China(No.823RC472)+4 种基金the Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2021WNLOKF008)the Hainan University Scientific Research Foundation(No.KYQD(ZR)19107)Natural Science Research Talent Project of Hainan Medical University(No.JBGS202101)Hainan Province Clinical Medical Center(2021)Project for Functional Materials and Molecular Imaging Science Innovation Group of Hainan Medical University。
文摘Triphenylamine(TPA)-based aggregation-induced emission luminogens(TPA-AIEgens),a type of photoactive material utilizing the typical TPA moiety,has recently attracted increasing attention for the diagnostics and treatment of tumors due to their remarkable chemo-physical performance in optoelectronic research.TPA-AIEgens are distinguished from other photoactive agents by their strong fluorescence,good sensitivity,high signal-to-noise ratio,resistance to photobleaching,and lack of high concentration or aggregation-caused fluoresce quenching effects.In this review,we summarize the current advancements and the biomedical progress of TPA-AIEgens in tumor theranostics.First,the design principles of TPAAIEgens photoactive agents as well as the advanced targeting strategies for nuclei,cell membranes,cell organelle and tumors were introduced,respectively.Next,the applications of TPA-AIEgens in tumor diagnosis and therapeutic techniques were reviewed.Last,the challenges and prospects of TPA-AIEgens for cancer therapy were performed.The given landscape of the TPA-AIEgens hereby is meaningful for the further design and utilization of the novel photoactive material,which could be beneficial for the development of clinic applications.
基金National Natural Science Foundation of China(Nos.21663005,21871060,21804022 and 21702016)the Natural Science Foundation of Jiangxi Province(Nos.2018ACB21009,20181BAB213007)+2 种基金the Science and Technology Project of the Education Department of Jiangxi Province of China(No.GJJ170846)the Special Graduate Student Innovation Fund of Jiangxi Province(No.YCX18B007)Beijing National Laboratory for Molecular Sciences(No.BNLMS201813)for the financial support
文摘An AIEgen decorated porphyrin(TPETPyP)was easily obtained through a one-step reaction.The bulky TPE in TPETPyP greatly impeded the intermolecularπ-πstacking of the porphyrin core,which significantly suppressed aggregation-caused quenching(ACQ)effect of TPETPyP in aqueous solution.The four pyridinium salts formed in TPETPyP also render the whole molecule water solubility,which eliminated its aggregation.TPETPyP exhibited 1O2 quantum yield as high as 0.85 in PBS.Moreover,it also showed high binding affinity to proteins,the major biotarget of 1O2.The high 1O2 quantum yield plus the great binding ability of TPETPyP toward proteins makes it a highly-efficient protein photocleaving agent.Protein electrophoresis experiments demonstrated that TPETPyP can photocleave BSA upon visible light irradiation,indicating that TPETPyP can act as a promising photosensitizer(PS)in PDT.The work here will provide a facile strategy to utilize AIEgens modified traditional PSs for photodynamic therapy(PDT).
基金supported by the National Natural Science Foundation of China(NSFC,No.22065031)the Key R&D Program of Gansu Province(No.21YF5GA066)+4 种基金Gansu Province College Industry Support Plan Project(No.2022CYZC-18)Natural Science Foundation of Gansu Province(Nos.2020-0405-JCC-630,20JR10RA088)Fundamental Research Funds for the Central Universities(Nos.31920190041,31920200002,31920190018,31920190013)Young Doctor Foundation of Gansu Province(No.2021QB-148)The Science and Technology Project Funded by Social Capital in Longnan City of Gansu Province(No.2021-SZ-01)。
文摘In recent twenty years,aggregation-induced emission(AIE),due to its excellent application prospect,has aroused widespread interests.The development of novel and easy to make AIE luminogens(AIEgens)is an attractive subject.For this purpose,it is very important to study the structure-property relationship of AIEgens.Because azine derivatives are easy to synthesis and some of them have nice AIE properties,herein,a series of azine derivatives(ADs)were employed as models to study the influence of different functional groups,electronic effects and structures on the AIE properties of azine derivatives.The AIE mechanism were studied by single crystal analysis,density functional theory(DFT)calculations and so on.The results indicated that the o-hydroxyl aryl substituted azine compounds could show good AIE properties.Meanwhile,the AIE properties of o-hydroxyl aryl substituted azine compounds were also influenced by the electronic effects of the aryl groups in the azine compounds.The o-hydroxyl groups could form intramolecular hydrogen bond with imine group,which play key role to restrict the intramolecular rotation of the aryl groups and act as base stone for the AIE process of this kind compounds.The HOMO-LUMO energy gaps of o-hydroxyl substituted azine are smaller than other homologous compounds,which is agree with the proposed AIE mechanism.Finally,thanks to the AIE properties,the o-hydroxy-substituted azines could be used as efficient Al^(3+)and Cu^(2+)fluorescent chemosensors in different conditions.In addition,test strips based on AD10 has been prepared,which can conveniently detect Cu^(2+)in industrial wastewater.This research supplied a way for the design of novel easy to make AIEgens through simple azine derivatives.
基金This work is supported by the Science and Technology Innovation Commission of Shenzhen,China(Nos.KQJSCX20180328095517269 and JCYJ20170818143131729).
文摘Aggregation-induced emission luminogens(AIEgens) have been used in biomacromolecules detection.Herein,TPE-dC and TPE-dU acted as the nucleoside-based AIEgens sensors in the first case,which can be used to detect ctDNA and rRNA in vitro and light up the nucleus in vivo depending on the intermolecular binding affinity.This AIE process enables the quantitative analysis or visualization of nucleic acids in solution or gels state,respectively.Furthermore,confocal laser scanning microscopy(CLSM) images of L929 cells stained with TPE-dC or TPE-dU clearly shows that nucleoside-based AIEgens bio-probes can pass the cell membranes to reach the cell nucleus,without cytotoxicity at the imaging condition(incubation time> 12 h,and 10 μmol/L of concentration).Since the nucleus is rich in DNA/RNA,fluorescence turn-on mode has a great potential in nucleus imaging and clinical diagnosis.
基金supported by the National Natural Science Foundation of China(No.52173152)the Fund of the Rising Stars of Shaanxi Province(No.2021KJXX-48)+2 种基金Scientific and Technological Innovation Team of Shaanxi Province(No.2022TD-36)The Natural Science Basic Research Plan in Shaanxi Province of China(No.2024JC-YBMS-123)Industrial Science and Technology Plan in Shaanxi Provincial Education Department(No.23JC001)。
文摘Supramolecular architectures exhibiting cascade energy transfer characteristics represent pivotal model systems for advancing biomimetic light-harvesting systems(LHS)that emulate the natural photosynthesis.To now,the engineering of aqueous-phase artificial LHS with optimized energy transfer cascades is still a challenge.In this study,we designed and synthesized two tetraphenylethylene(TPE)-based macrocyclic compounds(namely TPE-1 and TPE-2)with different cavity sizes as supramolecular scaffolds to study their energy transfer behaviors.As a control model,a linear molecule TPE-3 was also prepared.The bigger-cavity macrocycle TPE-1 can emit green fluorescence and self-assemble into nanospherical structures in aqueous media,acting as an energy donor.Through self-assembly with eosin Y(EY)and a red-emitting fluorophore(TPE-Se),a sequential Forster resonance energy transfer(FRET)cascade:TPE-1→EY→TPE-Se was achieved thanks to their excellent spectral overlap and proximity between the donor and acceptors.The optimized ternary system(TPE-1/EY/TPE-Se)with a ratio of 1000:90:60 afforded a high energy transfer efficiency(Φ_(ET))of 95%.Then,the artificial LHS platform catalyzed the oxidative coupling of benzylamines with 93%yield in aqueous media.Moreover,the system demonstrated broad catalytic utility oxidation reactions,the good conversion of methylthiobenzyl ester to methylbenzene sulfoxide and the aerobic cross-dehydrogenation coupling reaction of N-phenyltetrahydroisoquinoline with indole.These results robustly demonstrate the promising potential of this artificial LHS in the field of aqueous photocatalysis.
基金supported by the National Natural Science Foundation of China(No.21501063)the Science and Technology Development Program of Jilin Province(No.20180520015JH)the Open Funds of National Engineering Research Center of Near-Net-Shape Forming for Metallic Materials(2018011)。
文摘Theranostics,possessing both imaging and therapeutic functions,has emerged as a promising strategy for the efficient treatment of cancer.Aggregation-induced emission luminogen(AIEgen)functionalized inorganic-organic hybrid nanomaterials have attracted great interest in biomedicine due to their distinct advantages,such as good biocompatibility,excellent fluorescence properties,facile functionalization,etc.
基金supported by the State Basic Research Project of China(Grant No:2014CB931802)the National Natural Science Foundation of China(Grant No.:21320102001 and 21501063)the 111 project.
文摘Inorganic bioactive nanomaterials play a significant role in biomedical fields due to their excellent biocompatibility.AIEgen-functionalised mesoporous bioactive glass(FMBG)nanoparticles with sizes of 60-100 nm are prepared by post-grafting tetraphenylethene onto bioactive glass.The obtained nanoparticles emit strong blue light at 482 nm upon UV irradiation,showing potential application in cell imaging.Furthermore,the FMBG,with a high DOX loading capacity of 250.74μg mg^(-1)and good degradability in an acid environment.
基金supported by the State Basic Research Project of China(Grant No.:2014CB931802)the National Natural Science Foundation of China(Grant No.:51627805,21320102001,21621001,and 21501063)the 111 project(B17020).
文摘Aggregation-induced emission luminogen(AIEgen)-functionalised mesoporous silica nanoparticles were synthesized by post-grafting two types of tetraphenylethene derivatives on mesoporous materials.The fluorescent chemical sensors,denoted as FMSN-1 and FMSN-2,emitted strong blue fluorescence under UV irradiation.Both of them exhibited quick and sensitive responses to 2,4,6-trinitrophenol(PA)and 2,4-dinitrophenol(2,4-DNP)via a fluorescence quenching process.In particular,the obtained materials also showed remarkable sensing ability for the antibiotics furazolidone(FZD)and nitrofurazone(NF)with low detection limits.The highly efficient sensing abilities of FMSNs for PA,2,4-DNP,FZD,and NF can be attributed to fluorescence resonance energy transfer(FRET)from AIEgens to analytes.These results demonstrate the potential value of AIEgen-functionalised mesoporous materials in environmental protection.
文摘Aggregation-induced emission luminogens(AIEgens),tetraphenylethene,were post grafted onto mesoporous silica nanoparticles to form AIEgen-functionalised mesoporous materials.The obtained blue-emitting materials can serve as a fluorescence resonance energy transfer(FRET)donor for monitoring the release process of doxorubicin hydrochloride(DOX)as an acceptor by the change of the fluorescent signal,showing potential application in imaging-guided therapy.
文摘Ruthenium polypyridyl complexes are known for their excellent photophysical properties and rich electrochemical behavior.Thus,developing complexes with multifunctional behavior showing potential applications in multiple fields is of great importance.Herein,we report a new aggregation-induced emission(AIE)active bis-heteroleptic ruthenium(Ⅱ)complex(Ru-4)of 4,7-diamino 1,10-phenanthroline along with a series of other bis-heteroleptic Ru(Ⅱ)polypyridyl complexes(Ru-1,Ru-2 and Ru-3)containing 4,7-disubstituted 1,10-phenanthroline and two homoleptic complexes(Ru-5 and Ru-6)of 2,2’-bipyridine and 1,10-phenanthroline.
基金supported by the National Natural Science Foundation of China(52350002,52150222,U2330106,and 52073163)the Special Fund of Taishan Scholars Project of Shandong Province(tsqnz20231253)+1 种基金Natural Science Foundation of Shandong Province(ZR2024QB200,ZR2023ZD41)Fundamental Research Funds for the Central Universities(2022JC003).
文摘Apoptotic vesicles(ApoVs)are membrane structures formed during cell apoptosis and play crucial roles in homeostasis maintenance,signal transduction,and immune regulation.Importantly,ApoVs inherit the properties and contents of parental cells that show great potential in the diagnosis and treatment of diseases.Monitoring the formation process of ApoVs(such as quantity,morphological changes,release rules,etc.)can reveal the regulatory mechanism of apoptosis,and is also helpful for optimizing the preparation and application of ApoVs.However,due to the limitations of existing technologies,the formation processes of ApoVs have been challenging to precisely and entirely capture.Herein,we subtly constructed a versatile AIEgen(ADTP)that could induce ApoVs production and in situ monitor the formation process,and it was successfully applied to explore the formation mechanism of ApoVs.ADTP specifically targeted the plasma membrane,and it could effectively induce apoptosis under laser irradiation,so it was able to dynamically monitor the entire formation process of ApoVs and had validated ApoVs formation from membrane protrusions(including filopodia,tunneling nanotubes,and retraction fibers).Further investigation revealed that ApoVs derived from membrane protrusions with different components exhibited significant heterogeneity.Additionally,the nearinfrared emission characteristic of ADTP was compatible with the stimulated emission depletion(STED)microscopy equipped with a 775nmdepletion laser,enabling high-resolution visualization of detailed dynamic changes inmembrane protrusions during ApoVs formation.This work provided powerful tools for tracking the entire ApoVs formation process and also offered crucial scientific evidence for revealing the ApoVs formation mechanism.
基金supported by the National Natural Science Foundation of China(21525523,21574048,21375042,21405054)the National Basic Research Program of China(2015CB932600,2013CB933000)+1 种基金the Special Fund for Strategic New Indus-try Development of Shenzhen,China(JCYJ20150616144425376)1000 Young Talent(to Fan Xia)
文摘Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.
基金supported by the National Key R&D Program of China(no.2018YFE0205400)the National Natural Science Foundation of China(nos.51725302 and 31870998)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(no.XDB36000000)the Beijing Nova Program of Science and Technology(no.Z191100001119091).
文摘Luminogens with aggregation-induced emission(AIEgens)have a wide range of biomedical applications in bioimaging,photodynamic anticancer,antibacterial therapy,and other fields,owing to their unique photophysical properties.The precise structural design and modification of AIE molecules have aroused great interest in the past years.As peptides-AIE hybrid materials,peptide-based AIEgens generally have better solubility,biocompatibility,and lower systemic toxicity.The functional diversity,modularity,and portability of peptides provide more possibilities for the intelligent structure and functional design of AIEgens.This review summarizes the recent research progress of peptide-based AIEgens nanomaterials,from molecular design,stimuli responsiveness to biomedical application,focusing on the advantages of peptides and AIE molecules as conjugates.Finally,a summary of the challenges and opportunities of peptide-based AIEgens nanomaterials for future clinical biomedical applications is presented.
基金supported by the National Natural Science Foundation of China(21788102,52003228)the Innovation and Technology Commission(ITC-CNERC14SC01)+1 种基金the Research Grants Council of Hong Kong(16307020,C6009-17G,C6014-20W,and N-HKUST609/19)the Natural Science Foundation of Guangdong Province(2019B121205012)。
文摘Molecular interactions are crucial in diverse fields of protein folding,material science,nanotechnology,and life origins.Although mounting experimental research controls luminescent behavior by adjusting molecular interactions in light-emitting materials,it remains elusive to correlate microscopic molecular interactions with macroscopic luminescent behavior directly.Here,we synthesized three red luminogens with subtle structural variation and investigated the influence of molecular interactions on their luminescent behavior in solution and aggregate states.Our results indicate that strongπ-πand D-A interactions in both dilute solution(between luminogen and solvent molecules)and aggregate(between luminogens)states cause the redshift in emission,while weak interactions(e.g.,Van der Waals,C–H…π,and C–H…F interactions)enhance the quantum yield.This work provides a thoughtful investigation into the complicated influence of various molecular interactions on luminescent behavior.
基金supported by the National Natural Science Foundation of China(21375042,21405054,21525523,21574048,and21404028)the National Basic Research Program of China(2015CB932600,2013CB933000,and 2016YFF0100800)+1 种基金the Special Fund for Strategic New Industry Development of Shenzhen,China(JCYJ20150616144425376)1000 Young Talent Program(to F.Xia)
文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.
基金supported by the Topnotch Talents Program of Henan Agricultural University(30501032)the National Natural Science Foundation of China(52003228 and 52273197)+2 种基金the Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ2021324134613038)the Shenzhen Key Laboratory of Functional Aggregate Materials(ZDSYS20211021111400001)Shenzhen Peacock Team Project(KQTD20210811090142053).
文摘A new class of near-infrared(NIR)fluorescent organoboron AIEgens was successfully developed for latent fingerprints(LFPs)imaging.They exhibit real-time and in situ high-resolution imaging performance at 1-3 levels of LFPs by spraying method.In addition,we systematically elucidate the fingerprint imaging mechanism of these AIEgens.Significantly,the excellent level 3 structural imaging capabilities enable the application of them for analyzing incomplete LFPs and identifying individuals in different scenarios.
基金National Natural Science Foundation of China,Grant/Award Numbers:21571079,21621001,21390394,21571076,21571078″111″project,Grant/Award Numbers:B07016,B17020JLU Science and Technology Innovative Research Team。
文摘Aggregation-induced emission-based luminogens(AIEgens)have aroused enormous interest due to their unique high fluorescence in a condensed state.To further explore their potential applications,such as chemical monitoring,immobilization of AIE molecules has been widely studied with a variety of supports.Crystalline porous materials,such as metal-organic frameworks,covalent organic frameworks,hydrogen-bonded organic framework,and organic cages,demonstrate well-controlled structures,large surface areas,and promising stabilities,thus providing a perfect platform for AIE agents loading.Outstanding chemical sensing performances are achieved based on these AIE-active crystalline porous materials,such as high sensitivity,short response time,selective identification,and high recyclability,which provide a new alternative to readily detect various hazardous molecules.Furthermore,precise structures of AIEgen-based crystalline porous materials offer an easy way to investigate detection mechanisms.This mini-review will provide a brief overview of AIEgen-based crystalline porous materials for detection and then address how to improve sensing performances remarkably.
基金National Research Foundation of Korea,Grant/Award Number:2012R1A3A2048814National Natural Science Foundation of China,Grant/Award Number:22090011。
文摘Activity-based approaches for designing AIEgens possess prominent advantages including high selectivity,sensitivity,and signal-to-noise ratio,and they have received more attention in recent years.Excellent activatable AIE probes have been reported for detecting toxic substances,imaging intracellular active molecules/biomolecules,as well as monitoring the activity of overexpressed enzymes in cancers.Moreover,the majority of activatable theranostic AIEgens can be specifically triggered in cancer cells and can kill these cells under light irradiation,while they have no distinct effect on normal cells,demonstrating satisfactory therapeutic selectivity that is superior to that of traditional chemotherapy.Thus,in this review,we systematically summarized the development of activatable AIE bioprobes in recent years from molecular design principles to biological applications.The challenges of activatable AIE probes and the corresponding solutions are described.We hope that the information provided in this review will facilitate the design of more activatable AIE probes to promote practical application of corresponding AIEgens.
