Observations of the micro world,especially the structures of organelles,have been attractive topics since the 17th century.As a powerful detection tool,the fluorescence technique has played a significant role in bioim...Observations of the micro world,especially the structures of organelles,have been attractive topics since the 17th century.As a powerful detection tool,the fluorescence technique has played a significant role in bioimaging to provide more details and enhance the signal-to-noise ratio compared to that of traditional optical microscopes.The boom of aggregate-induced emission luminogens(AIEgens)in the last two decades has revolutionized the design strategy of luminescent materials for biological applications.This Review summarizes the advantages and recent progress of AIEgens in imaging and tracking.Different imaging strategies of AIEgens including turn-on imaging,stimuli-response sensing,and longterm tracking are presented.NIR AIEgens used for in-depth bioimaging via different methods are also discussed.Finally,we propose several potential development directions for AIEgens in bioimaging.展开更多
基金supported by the National Natural Science Foundation of China(52003228,52273197,and 21788102)Shenzhen Key Laboratory of Functional Aggregate Materials(ZDSYS20211021111400001),the Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20220818103007014,JCYJ2021324134613038,JCYJ20220530143805012,KQTD20210811090142053,JSGG20220606141800001,GJHZ20210705141810031)+3 种基金the China Postdoctoral Science Foundation(2022M713022)the Open Fund of Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates(2021-kllma-08)Guangzhou 510640,China(South China University of Technology)Guangdong Basic and Applied Basic Research Foundation(2023A1515011342).
文摘Observations of the micro world,especially the structures of organelles,have been attractive topics since the 17th century.As a powerful detection tool,the fluorescence technique has played a significant role in bioimaging to provide more details and enhance the signal-to-noise ratio compared to that of traditional optical microscopes.The boom of aggregate-induced emission luminogens(AIEgens)in the last two decades has revolutionized the design strategy of luminescent materials for biological applications.This Review summarizes the advantages and recent progress of AIEgens in imaging and tracking.Different imaging strategies of AIEgens including turn-on imaging,stimuli-response sensing,and longterm tracking are presented.NIR AIEgens used for in-depth bioimaging via different methods are also discussed.Finally,we propose several potential development directions for AIEgens in bioimaging.
