Strain Z35,isolated from the rhizosphere of healthy Panax pseudoginseng under long-term continuous cropping,exhibits strong antagonistic activity against Fusarium oxysporum and shows potential as a biocontrol agent.Ho...Strain Z35,isolated from the rhizosphere of healthy Panax pseudoginseng under long-term continuous cropping,exhibits strong antagonistic activity against Fusarium oxysporum and shows potential as a biocontrol agent.However,the genetic and metabolic basis of its antimicrobial and plant growth-promoting functions remain unclear.In this study,plate confrontation assays confirmed that Z35 inhibited F.oxysporum by up to 75%.Whole-genome sequencing using the PacBio platform revealed a 38.65 Mb genome with seven contigs and one scaffold,a GC content of 47.48%,and 99.9%completeness(BUSCO).Phylogenetic analysis identified Z35 as Trichoderma koningiopsis.Genome annotation uncovered 49 secondary metabolite biosynthetic gene clusters and 717 functionally relevant genes,including clusters related to nonribosomal peptide synthetases(NRPS),polyketide synthases(PKS),and terpenes.These clusters are potentially responsible for producing bioactive compounds such as clavaric acid,the metachelin series,dimerumic acid,ascochlorin,and trichoxide.Non-targeted metabolomic profiling validated the presence of these metabolites in Z35 fermentation products,supporting their role in antifungal activity and plant-beneficial effects.This study provides insights into the genetic and metabolic mechanisms underlying Z35's biocontrol potential,offering a theoretical basis for its further development and application in sustainable agriculture.展开更多
基金supported by the National Key Research and Development(2021YFD1601003)the Project of The Sino-Vietnamese International Joint Laboratory for Characteristic&Cash Crops Green Development of Yunnan Province(202403AP140013)Development and Application of Personalized Cigarette Products'(2023CP02).
文摘Strain Z35,isolated from the rhizosphere of healthy Panax pseudoginseng under long-term continuous cropping,exhibits strong antagonistic activity against Fusarium oxysporum and shows potential as a biocontrol agent.However,the genetic and metabolic basis of its antimicrobial and plant growth-promoting functions remain unclear.In this study,plate confrontation assays confirmed that Z35 inhibited F.oxysporum by up to 75%.Whole-genome sequencing using the PacBio platform revealed a 38.65 Mb genome with seven contigs and one scaffold,a GC content of 47.48%,and 99.9%completeness(BUSCO).Phylogenetic analysis identified Z35 as Trichoderma koningiopsis.Genome annotation uncovered 49 secondary metabolite biosynthetic gene clusters and 717 functionally relevant genes,including clusters related to nonribosomal peptide synthetases(NRPS),polyketide synthases(PKS),and terpenes.These clusters are potentially responsible for producing bioactive compounds such as clavaric acid,the metachelin series,dimerumic acid,ascochlorin,and trichoxide.Non-targeted metabolomic profiling validated the presence of these metabolites in Z35 fermentation products,supporting their role in antifungal activity and plant-beneficial effects.This study provides insights into the genetic and metabolic mechanisms underlying Z35's biocontrol potential,offering a theoretical basis for its further development and application in sustainable agriculture.
