Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major coh...Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major cohort for stem cell-based therapies.However,the regenerative potential of stem cells significantly decreases with advanced age and deteriorating health status of the donor.Therefore,the efficacy of autologous stem cell therapy is significantly compromised in older patients.To overcome these limitations,alternative strategies have been used to restore the age-and disease-depleted function of stem cells.These methods aim to restore the therapeutic efficacy of aged stem cells for autologous use.This article explores the effect of donor age and health status on the regenerative potential of stem cells.It further highlights the limitations of stem cell-based therapy for autologous treatment in the elderly.A comprehensive insight into the potential strategies to address the“age”and“disease”compromised regenerative potential of autologous stem cells is also presented.The information provided here serves as a valuable resource for physicians and patients for optimization of stem cellbased autologous therapy for aged patients.展开更多
Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of n...Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of neurodegenerative diseases with glaucoma being highly prevalent.Glaucoma is characterized by the progressive dysfunction and loss of retinal ganglion cells and their axons which make up the optic nerve.It is the leading cause of irreversible vision loss and affects an estimated 80 million people.The mammalian central nervous system is non-regenerative and,once lost or injured,retinal ganglion cells cannot regenerate an axon into the optic nerve under basal conditions.Thus,strategies that provide neuroprotection to stressed,dysfunctional,or dying retinal ganglion cells are likely to be of high therapeutic and translational value.Advancing age,genetics,and elevated intraocular pressure are all major risk factors for glaucoma,however,all clinically available glaucoma treatments focus on intraocular pressure management and do not directly address the neurodegenerative component of glaucoma.展开更多
Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived mi...Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived miRNAs may play a key role in modulating embryonic oxidation-reduction process,whereas the exact function and mechanism were still poorly understood.The present study aimed to investigate the roles of egg exosome miRNAs in maintaining dynamic equilibrium of free radicals and peroxide agents in embryonic liver,as well as demonstrate the specific mechanism using oxidative stress-challenged hepatocytes.Results Compared to 36-week-old breeding hens,decreased hatchability and increased embryonic mortality were observed in 65-week-old breeding hens.Meanwhile,the older group showed the increased MDA levels and decreased SOD and GSH-Px activities in embryonic liver,muscle and serum.Embryonic mortality was significantly positively correlated with MDA level and negatively correlated with GSH-Px activity in embryonic liver.In addition,363 differentially expressed genes(DEGs)were identified in embryonic liver,13 differentially expressed miRNAs(DE-miRNAs)were identified in egg exosomes.These DEGs and DE-miRNAs were involved in oxidoreductase activity,glutathione metabolic process,MAPK signaling pathway,apoptosis and autophagy.miRNA-mRNA network analysis further found that DEGs targeted by DE-miRNAs were mainly enriched in programmed cell death,such as apoptosis and autophagy.Wherein,MAPK10 with highest MCC and AUC values was significantly related to GSH-Px activity and MDA level,and served as the target gene of miR-145-5p based on dual luciferase reporter experiment and correlation analysis.Bioinformatics analysis found that miR-145-5p/MAPK10 axis might alleviate peroxide generation and apoptosis.In primary hepatocytes of chick embryos,miR-145-5p transfection significantly reversed H_(2)O_(2)-induced mitochondrial ROS increase,MAPK10,BAX and CASP3 overexpression and excessive apoptosis.Conclusion Exosome miR-145-5p in eggs could target MAPK10 and decrease mitochondrial ROS,attenuating oxidative damage and apoptosis in hepatocytes of chick embryos.These findings may provide new theoretical basis for the improvement of maternal physiological status to maintain embryonic redox homeostasis by nutritional or genetic modifications.展开更多
Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted ...Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted on problems in middle-aged people have focused on visual disorders without taking into account all the ocular morbidities that may affect this segment of the population, hence the present study, the aim of which is to determine the proportions of different eye diseases in people aged 40 and over. Materials and Methods: This was a descriptive cross-sectional study carried out in the ophthalmology department covering the period from January 1 to December 31, 2020. Results: In total, we collected 828 patients aged 40 and over out of 1811 patients who received ophthalmological consultation during the study period, representing 45.72%. The most represented age group was 40 - 50 years, with an average age of 58.84 years and a maximum of 93 years. There were slightly more women (62.3%) than men (37.7%). The main reasons for consultation were decreased visual acuity (26.4%) and pruritus (19.9%). The main eye diseases diagnosed were cataracts (23%), allergic conjunctivitis (21.1%), and bacterial conjunctivitis (14.2%). Discussions: The predominance of cataracts in the diagnosed diseases confirms the literature data, according to which the main eye morbidities in middle-aged and elderly people are cataracts, glaucoma, and age-related macular degeneration. Conclusion: It is crucial to have a mastery of these epidemiological data of eye diseases in order to adapt the technical platforms of eye care structures to the needs of different segments of the population.展开更多
Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mR...Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mRNA transcriptomics was used to analyze differentially expressed genes and ferroptosis markers.Next,a weighted correlation network analysis was conducted on these genes.Then,common genes among ferroptosis,Yinyanghuo,and differentially expressed genes were identified as target genes.Single-cell and spatial transcriptomics were analyzed to evaluate expression changes of target genes in bone marrow.Furthermore,to validate the results of bioinformatics analysis,MC3T3-E1 cells were used to model the preventive effects of icariin in vitro,and ferroptosis markers and mitochondrial function were both examined.In vivo,4-month-old mice were fed a diet containing icariin for 14 months,following which bone proteomics was assessed to identify essential proteins.Hematoxylin-eosin staining and immunohistochemistry assays were performed on mouse femurs.Finally,Western blot and polymerase chain reaction(PCR)analyses were performed to analyze the effects of icariin on ferroptosis target biomarkers.Ptgs2 and Hmox1 were identified as target genes related to both icariin and ferroptosis.The expression of ferroptosis-related genes was up-regulated with age.Moreover,single-cell and spatial transcriptomics analyses revealed that up-regulation of these two genes inhibited osteogenic capability.The results of the in vitro experiments indicated that icariin mitigated the accumulation of reactive oxygen species and β-galactosidase.Similarly,icariin prevented aberrant changes in the levels of ferroptosis-related proteins,consistent with the results of the in vivo experiments.Specifically,10 mg/(kg·day)of icariin inhibited ferroptosis and osteoporosis in aged mice.Overall,this study revealed that icariin could serve as a dietary supplement to prevent age-induced osteoporosis.Furthermore,Ptgs2 and Hmox1 were identified as ferroptosis-related targets through which icariin exerts its protective effects.展开更多
Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.H...Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.However,the status and influencing factors of these behaviors among community-dwelling older adults in China remain poorly understood.This study aimed to investigate fall prevention self-management behaviors and their influencing factors among community-dwelling older adults in Fuzhou,China.Methods:From June to December 2022,we used multi-stage cluster random sampling and invited 675 community-dwelling older adults from Fujian as participants.We conducted a questionnaire survey using the general information questionnaire,Fall Prevention Self-management Behavior among Older Adults Questionnaire,Questionnaire on Knowledge,Belief,and Practice of Falls Prevention among Older Adults in Community,Modified Falls Efficacy Scale,Social Support Rating Scale,and Chinese Version of the Self-Assessment Fall Risk Scale for Older Adults.Multivariate linear regression was used to identify the factors influencing fall prevention self-management behavior among older adults.Results:The overall score of fall prevention self-management behaviors was 146.81±34.22,indicating a moderate level.Key influencing factors included education level,pre-retirement occupation,income,medication type,self-assessed physical condition,fear of falling,knowledge and practices in fall prevention,social support,and fall risk.These factors collectively explained 34.3%of the variance in self-management behaviors.Conclusion:The findings highlight the need for targeted interventions to enhance fall prevention self-management among community-dwelling older adults.Interventions should focus on improving knowledge,strengthening social support,and addressing psychological barriers such as fear of falling.This study provides valuable insights for developing community-based strategies to reduce fall risks and promote healthy aging.展开更多
BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of t...BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of the gut.Loss of miR-10b-5p in ICC im-pairs intestinal motility and contributes to constipation,a common condition in the elderly.Notably,miR-10b-5p is co-expressed with its paralog,miR-10a-5p,in ICC.AIM To investigate the roles of miR-10a-5p and miR-10b-5p in age-associated intestinal dysmotility and assess the therapeutic potential of restoring their expression.METHODS We employed aged mice,mir-10a and mir-10b single and double knockout(KO)models,and human plasma and colon samples across age groups.GI and colonic transit,ICC network integrity,and expression levels of miR-10a/b-5p were eva-luated.Additionally,we tested whether treatment with their microRNA mimics could restore GI motility in aged mice.RESULTS Aged mice exhibited delayed GI and colonic transit,reduced fecal output,and diminished expression of miR-10a-5p and miR-10b-5p,which peaked during late embryonic and early postnatal stages and declined with age.This decline para-lleled ICC network deterioration in the colon.All KO models exhibited impaired motility and ICC loss,with mir-10a KO mice displaying more severe phenotypes than mir-10b KO mice.Double KO mice demonstrated growth retardation and reduced survival,with homozygous mutants living only up to 3 months.Treatment of aged mice with miR-10a-5p and miR-10b-5p mimics encapsu-lated in jetPEI significantly improved GI and colonic motility.Successful delivery to the gut,including the colon,was confirmed.In human samples,both miR-10a/b-5p and KIT expression decreased with age.CONCLUSION miR-10a-5p and miR-10b-5p are essential for ICC maintenance and colonic motility,and their age-related decline contributes to GI dysmotility in both mice and humans.Restoring their levels offers a promising therapeutic stra-tegy for treating age-related constipation and other motility disorders.展开更多
The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,p...The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,potentially affecting their interactions with pollutants.This study aims to compare the combined toxicity effects of virgin and aged MFs with the common heavy metal copper(Cu).Gibel carp Carassius auratus gibelio were exposed to 1 mg/L of virgin and aged polyacrylonitrile MFs(PanMfs)separately and in combination with Cu(0.5-mg/L copper sulfate)for 21 d.Results show that both aged and virgin PanMfs significantly reduced Cu accumulation in gibel carp,with no notable difference.Cu adversely affected the carp's intestine and liver,while PanMfs alone had minimal histological impact but notably mitigated intestinal damage caused by Cu.Enzyme activity and gene expression analysis indicated that both PanMfs and Cu exposure,alone or combined,compromised gibel carp's antioxidant capacity,digestive and liver functions,induced inflammation,and affected liver metabolism and intestinal immune response.Integrated biomarker response analysis of enzyme activity and gene expression showed that combined Cu and PanMfs exposure had a stronger impact on gibel carp than individual exposure.However,whether exposed individually or in combination,aged PanMfs had a slightly greater impact than virgin PanMfs.This study showed that PanMfs alone exhibit minimal toxic effects on gibel carp but significantly modify Cu toxicity,with negligible differences between aged and virgin PanMfs except for slight disparities in enzyme activity and gene expression.展开更多
The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of b...The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of bio-oils and their component correspondence with petroleum asphalt were compared.The diffusion and fusion effects of various bio-oils in aged asphalt were introduced.Bio-oil cannot be used as a direct alternative of petroleum asphalt,but it has the potential to effectively rejuvenate aged asphalt binders due to the component similarity with petroleum asphalt and good diffusion properties.For the asphalt rejuvenation,the functionalization treatment methods of bio-oil were discussed such as purification,composition modification and component conversion.The active groups and derivatives in bio-oil can be converted into the missing components of the aged binder through phenolate,grafting,polycondensation,resinifying,but the conversion process and mechanism are still unclear.From the perspectives of diffusion behavior,components regulation,dissolving asphaltene and micro-rejuvenation effect,the rejuvenation behavior and mechanism of bio-oil on aged asphalt were elaborated,and the effects of various types of bio-oil and aged asphalt on rejuvenation behavior were analyzed.The preparation process and dosage of bio-rejuvenator were summarized.The rejuvenation effects of bio-oil on aged asphalt were comprehensively investigated from the aspects of high and low temperature performances,rheological properties,microstructure and chemical composition of bio-rejuvenated asphalt binders.Finally,the limitations of bio-oil used as asphalt rejuvenators were discussed,and future research directions were prospected,which can provide reference and theoretical basis for the development of high-performance bio-oil rejuvenating agents and the engineering application of bio-oil to improve the properties of aged asphalt materials.展开更多
The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack r...The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack resistance of mixture.To address the issue,dry-method styrene-butadiene-styrene(DSBS)and epoxy resin were mixed with aged asphalt to prepare SBS-modified epoxy reclaimed asphalt(SERA).The micro fusion characteristics and mechanical properties of SERA were evaluated,and the optimal DSBS dosage was determined based on various tests.The results show that adding DSBS can enable the tensile toughness and low-temperature performance of SERA with less EP content to reach or exceed the performance level of epoxy reclaimed asphalt(ERA)with higher EP content.At 30%EP content,the recommended dry-method SBS content is 9%;At 40%EP content,the recommended dry-method SBS content is 5%;When the EP content is 50%,the recommended dry-method SBS content is 7%.展开更多
Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yunga...Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yungang Grottoes,with their solemn Buddhas,stand as timeless sentinels,whispering tales of a millennium's passage.On the sacred slopes of Mount Wutai,the air is scented with incense,and the tranquil spirit of Zen envelops the misty landscape.Journey back to the heart of the Three Jins,where the aroma of aged vinegar lingers in the air,and hidden vistas reveal their profound beauty.Here,you will discover a charm and character like no other,as the spirit of the land unveils its unique poetic elegance and grace.展开更多
Hengyang City faces challenges from rapid aging,including weakened family care and a shortage of resources.The"three social linkage"mechanism integrates community of practice,social organizations,and profess...Hengyang City faces challenges from rapid aging,including weakened family care and a shortage of resources.The"three social linkage"mechanism integrates community of practice,social organizations,and professional support to improve health services for the aged.However,issues like low smart device use and talent shortages persist.This study aims to optimize resource allocation and service efficiency through a"chain+virtual elderly care"model.This article explores the"three social linkage"mechanism and the integration of chain services with virtual elderly care models in Hengyang City.It examines three core elements:Resource integration,service collaboration,and professional support.The community coordinates resources and maintains databases of elderly care needs,while social organizations provide services and social workers design care plans.Data was gathered through case studies,interviews,and observations,focusing on smart technology integration and service outcomes.The study also evaluates crossdepartmental data sharing,social worker training,and funding mechanisms,using performance indicators like service delivery time,user satisfaction,and resource use.The analysis of elderly care services in Hengyang City reveals key issues and improvements within the"three social linkage"mechanism.The three social linkage mechanism has facilitated better resource integration and service collaboration.Communities have created resource lists and service demand databases,improving coordination.Social organizations provide specialized services,and social workers conduct home visits to create personalized care plans.The virtual elderly care platform has enhanced service efficiency,with smart devices like bracelets enabling real-time health monitoring.The"chain+virtual elderly care"model in Hengyang has effectively addressed issues of scattered and outdated resources by integrating services through community hubs,standardized stations,and dynamic resource databases.Smart elderly care platforms,especially devices like wristbands,have improved service efficiency and emergency response.展开更多
Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducte...Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducted from October to December 2022 using a convenience sampling method.A total of 312 older adults were selected from 13 day service centers for older adults in Macao,China.The Advance Care Planning Acceptance Questionnaire and the Family Adaptation,Partnership,Growth,Affection,Resolve(APGAR)Scale were used to survey the older adults.Results:A total of 306 older adults completed the survey.The score for advance care planning readiness was 65.55±10.69,and 59.5%of participants(n=182)were willing to participate in ACP.The family function score was 7.24±2.51,while 70.3%of participants were from a highly functional family.The higher family function indicating a higher readiness for advance care planning(r=0.396,P<0.001).The multiple linear regression analysis indicated that the variables“age,”“knowledge of ACP,”“experience with ACP,”and“received resuscitation of yourself,relatives or friends”combined with“family function”can influence advance care planning readiness among older adults(R^(2)=0.317,F=27.898,P<0.001).Conclusions:Older adults in Macao’s day service centers were willing to engage in ACP.The importance of family involvement is highlighted in the ACP readiness.Health education and improved family communication are vital for promoting ACP,which ensures individuals receive care when they lack the capacity to make that choice.Additionally,healthcare professionals should enhance communication and education with older adults during the medical care process.展开更多
Alzheimer's disease(AD),the most prevalent form of dementia,disproportionately affects the elderly population.While aging is widely recognized as a major risk factor for AD,the precise mechanisms by which aging co...Alzheimer's disease(AD),the most prevalent form of dementia,disproportionately affects the elderly population.While aging is widely recognized as a major risk factor for AD,the precise mechanisms by which aging contributes to the pathogenesis of AD remain poorly understood.In our previous work,the neuropathological changes in the brains of aged cynomolgus monkeys(≥18 years old)following parenchymal cerebral injection of amyloid-β oligomers(AβOs)have been characterized.Here,we extend our investigation to middle-aged cynomolgus monkeys(≤15 years old)to establish an AD model.Surprisingly,immunohistochemical analysis reveals no detectable AD-related pathology in the brains of middle-aged monkeys,even after AβOs injection.In a comprehensive pathological analysis of 38 monkeys,we observe that the amyloid-β(Aβ)burden increases significantly with advancing age.Notably,the density of Aβ plaques is markedly higher in the ventral regions compared with the dorsal regions of aged monkey brains.Furthermore,we demonstrate that tau phosphorylation coincides with the accumulation of extensive Aβplaques and exhibits a positive correlation with Aβ burden in aged monkeys.Collectively,these findings underscore the critical role of the aged brain in providing the necessary conditions for AβO-induced AD pathologies in cynomolgus monkeys.展开更多
The Japanese encephalitis virus(JEV)causes Japanese encephalitis(JE),a severe disease that primarily affects children and induces significant central nervous system complications.With the widespread adoption of vaccin...The Japanese encephalitis virus(JEV)causes Japanese encephalitis(JE),a severe disease that primarily affects children and induces significant central nervous system complications.With the widespread adoption of vaccination in children,the incidence among older individuals has increased substantially.Despite this epidemiological shift,research on JEV infection in the elderly remains limited.We established JEV infection models using both aged and young mice to explore age-related differences in pathology and underlying mechanisms.Brain tissue samples were analyzed for pathological changes and viral tropism in major cell types.To further characterize immune response variations,we conducted transcriptomic sequencing on the brain tissues following JEV infection.Aged mice exhibited lower mortality,delayed disease progression,and milder brain pathology compared to young mice after JEV infection.Viral titers and infection rates of major brain cell types were similar in both groups.Transcriptomic analysis revealed diminished immune activation and weaker inflammatory responses in aged mice.Additionally,microglial activation and CD8^(+) T cell function were significantly reduced.Interestingly,JEV infection induced the selective recruitment of B cells in the brains of aged mice.These B cells may modulate the effects of CD8^(+) T cells in the disease process.Compared to young mice,aged mice showed enhanced resistance to JEV progression and reduced brain pathology.This resistance was associated with a weakened immune response in the aged brain,rather than differences in viral infection.The specific recruitment of B cells in the brains of aged mice may play a crucial role in limiting disease progression.展开更多
There is no way around it.Regular physical activity(PA)and a high-quality diet are essential to promoting good health and prolonging life in middle-aged and older adults.In the November 2024 Issue of the Journal of Sp...There is no way around it.Regular physical activity(PA)and a high-quality diet are essential to promoting good health and prolonging life in middle-aged and older adults.In the November 2024 Issue of the Journal of Sport and Health Science,Ding et al.展开更多
In 2019,China had over 13.14 million dementia cases,with incidence rates of(56.47–207.08)/100,000[1].Early cognitive impairment—a key dementia symptom—reduces quality of life,increases care dependence,and lowers su...In 2019,China had over 13.14 million dementia cases,with incidence rates of(56.47–207.08)/100,000[1].Early cognitive impairment—a key dementia symptom—reduces quality of life,increases care dependence,and lowers survival in older adults[2].A decline in physical function can also be observed in older adults with increasing age.Grip strength has been shown to be a marker of overall physiological function in older adults.展开更多
Background It remains unclear whether sleep duration and physical activity(PA)trajectories in middle-aged and older adults are associated with different risks of cardiovascular diseases(CVDs).This study aimed to explo...Background It remains unclear whether sleep duration and physical activity(PA)trajectories in middle-aged and older adults are associated with different risks of cardiovascular diseases(CVDs).This study aimed to explore the trajectories of total sleep duration and PA among middle-aged and older Chinese adults and their impact on CVD risk.Methods This study was based on the China Health and Retirement Longitudinal Study.12009 adults aged 45 years and older from five waves were included.CVD events were measured by self-reports of heart disease and stroke.We first used groupbased trajectory modeling to identify total sleep duration and PA trajectories from 2011 to 2020,and then employed logistic regression models to analyze their risk for CVD.Results We identified three sleep duration and PA trajectories.The risk of heart disease increased by 33%(OR=1.31,95%CI:1.12-1.53)for the short sleep duration trajectory(vs.moderate sleep duration trajectory),by 40%(OR=1.40,95%CI:1.06-1.84)for the high decreasing PA trajectory,and by 20%(OR=1.20,95%CI:1.01-1.42)for the low stable PA trajectory(vs.high stable PA trajectory),respectively.Similar results for stroke and CVD as the outcomes were also observed,but the higher risk of stroke in the high decreasing PA trajectory group was not statistically significant.The joint effects of sleep and PA showed lower risks of heart disease and stroke in trajectories with moderate or long sleep duration and high stable PA compared with short sleep duration and a low stable PA trajectory.Conclusions Short total sleep duration,high decreasing PA,and low stable PA trajectories could increase the risk of CVDs among middle-aged and older adults.Long-term moderate to long total sleep durations and high stable PA trajectories might be optimal for preventing CVDs.展开更多
文摘Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major cohort for stem cell-based therapies.However,the regenerative potential of stem cells significantly decreases with advanced age and deteriorating health status of the donor.Therefore,the efficacy of autologous stem cell therapy is significantly compromised in older patients.To overcome these limitations,alternative strategies have been used to restore the age-and disease-depleted function of stem cells.These methods aim to restore the therapeutic efficacy of aged stem cells for autologous use.This article explores the effect of donor age and health status on the regenerative potential of stem cells.It further highlights the limitations of stem cell-based therapy for autologous treatment in the elderly.A comprehensive insight into the potential strategies to address the“age”and“disease”compromised regenerative potential of autologous stem cells is also presented.The information provided here serves as a valuable resource for physicians and patients for optimization of stem cellbased autologous therapy for aged patients.
基金supported by St.Erik Eye Hospital philanthropic donations,Vetenskapsrådet 2022-00799(to PAW).
文摘Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of neurodegenerative diseases with glaucoma being highly prevalent.Glaucoma is characterized by the progressive dysfunction and loss of retinal ganglion cells and their axons which make up the optic nerve.It is the leading cause of irreversible vision loss and affects an estimated 80 million people.The mammalian central nervous system is non-regenerative and,once lost or injured,retinal ganglion cells cannot regenerate an axon into the optic nerve under basal conditions.Thus,strategies that provide neuroprotection to stressed,dysfunctional,or dying retinal ganglion cells are likely to be of high therapeutic and translational value.Advancing age,genetics,and elevated intraocular pressure are all major risk factors for glaucoma,however,all clinically available glaucoma treatments focus on intraocular pressure management and do not directly address the neurodegenerative component of glaucoma.
基金supported by China Agriculture Research System of MOF and MARA(CARS-40)the National Natural Science Foundation of China(32302776)。
文摘Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived miRNAs may play a key role in modulating embryonic oxidation-reduction process,whereas the exact function and mechanism were still poorly understood.The present study aimed to investigate the roles of egg exosome miRNAs in maintaining dynamic equilibrium of free radicals and peroxide agents in embryonic liver,as well as demonstrate the specific mechanism using oxidative stress-challenged hepatocytes.Results Compared to 36-week-old breeding hens,decreased hatchability and increased embryonic mortality were observed in 65-week-old breeding hens.Meanwhile,the older group showed the increased MDA levels and decreased SOD and GSH-Px activities in embryonic liver,muscle and serum.Embryonic mortality was significantly positively correlated with MDA level and negatively correlated with GSH-Px activity in embryonic liver.In addition,363 differentially expressed genes(DEGs)were identified in embryonic liver,13 differentially expressed miRNAs(DE-miRNAs)were identified in egg exosomes.These DEGs and DE-miRNAs were involved in oxidoreductase activity,glutathione metabolic process,MAPK signaling pathway,apoptosis and autophagy.miRNA-mRNA network analysis further found that DEGs targeted by DE-miRNAs were mainly enriched in programmed cell death,such as apoptosis and autophagy.Wherein,MAPK10 with highest MCC and AUC values was significantly related to GSH-Px activity and MDA level,and served as the target gene of miR-145-5p based on dual luciferase reporter experiment and correlation analysis.Bioinformatics analysis found that miR-145-5p/MAPK10 axis might alleviate peroxide generation and apoptosis.In primary hepatocytes of chick embryos,miR-145-5p transfection significantly reversed H_(2)O_(2)-induced mitochondrial ROS increase,MAPK10,BAX and CASP3 overexpression and excessive apoptosis.Conclusion Exosome miR-145-5p in eggs could target MAPK10 and decrease mitochondrial ROS,attenuating oxidative damage and apoptosis in hepatocytes of chick embryos.These findings may provide new theoretical basis for the improvement of maternal physiological status to maintain embryonic redox homeostasis by nutritional or genetic modifications.
文摘Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted on problems in middle-aged people have focused on visual disorders without taking into account all the ocular morbidities that may affect this segment of the population, hence the present study, the aim of which is to determine the proportions of different eye diseases in people aged 40 and over. Materials and Methods: This was a descriptive cross-sectional study carried out in the ophthalmology department covering the period from January 1 to December 31, 2020. Results: In total, we collected 828 patients aged 40 and over out of 1811 patients who received ophthalmological consultation during the study period, representing 45.72%. The most represented age group was 40 - 50 years, with an average age of 58.84 years and a maximum of 93 years. There were slightly more women (62.3%) than men (37.7%). The main reasons for consultation were decreased visual acuity (26.4%) and pruritus (19.9%). The main eye diseases diagnosed were cataracts (23%), allergic conjunctivitis (21.1%), and bacterial conjunctivitis (14.2%). Discussions: The predominance of cataracts in the diagnosed diseases confirms the literature data, according to which the main eye morbidities in middle-aged and elderly people are cataracts, glaucoma, and age-related macular degeneration. Conclusion: It is crucial to have a mastery of these epidemiological data of eye diseases in order to adapt the technical platforms of eye care structures to the needs of different segments of the population.
基金funded by the Natural Science Foundation of Hubei Province(2021CFB414)Scientific Research Program of Traditional Chinese Medicine of Hubei Provincial Health and Wellness Commission(ZY2021M074).
文摘Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mRNA transcriptomics was used to analyze differentially expressed genes and ferroptosis markers.Next,a weighted correlation network analysis was conducted on these genes.Then,common genes among ferroptosis,Yinyanghuo,and differentially expressed genes were identified as target genes.Single-cell and spatial transcriptomics were analyzed to evaluate expression changes of target genes in bone marrow.Furthermore,to validate the results of bioinformatics analysis,MC3T3-E1 cells were used to model the preventive effects of icariin in vitro,and ferroptosis markers and mitochondrial function were both examined.In vivo,4-month-old mice were fed a diet containing icariin for 14 months,following which bone proteomics was assessed to identify essential proteins.Hematoxylin-eosin staining and immunohistochemistry assays were performed on mouse femurs.Finally,Western blot and polymerase chain reaction(PCR)analyses were performed to analyze the effects of icariin on ferroptosis target biomarkers.Ptgs2 and Hmox1 were identified as target genes related to both icariin and ferroptosis.The expression of ferroptosis-related genes was up-regulated with age.Moreover,single-cell and spatial transcriptomics analyses revealed that up-regulation of these two genes inhibited osteogenic capability.The results of the in vitro experiments indicated that icariin mitigated the accumulation of reactive oxygen species and β-galactosidase.Similarly,icariin prevented aberrant changes in the levels of ferroptosis-related proteins,consistent with the results of the in vivo experiments.Specifically,10 mg/(kg·day)of icariin inhibited ferroptosis and osteoporosis in aged mice.Overall,this study revealed that icariin could serve as a dietary supplement to prevent age-induced osteoporosis.Furthermore,Ptgs2 and Hmox1 were identified as ferroptosis-related targets through which icariin exerts its protective effects.
基金the community of older adults who participated in this study.Ministry of Education Science and Technology Industry-University Co-operation Collaborative Education Project(230806630292310).
文摘Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.However,the status and influencing factors of these behaviors among community-dwelling older adults in China remain poorly understood.This study aimed to investigate fall prevention self-management behaviors and their influencing factors among community-dwelling older adults in Fuzhou,China.Methods:From June to December 2022,we used multi-stage cluster random sampling and invited 675 community-dwelling older adults from Fujian as participants.We conducted a questionnaire survey using the general information questionnaire,Fall Prevention Self-management Behavior among Older Adults Questionnaire,Questionnaire on Knowledge,Belief,and Practice of Falls Prevention among Older Adults in Community,Modified Falls Efficacy Scale,Social Support Rating Scale,and Chinese Version of the Self-Assessment Fall Risk Scale for Older Adults.Multivariate linear regression was used to identify the factors influencing fall prevention self-management behavior among older adults.Results:The overall score of fall prevention self-management behaviors was 146.81±34.22,indicating a moderate level.Key influencing factors included education level,pre-retirement occupation,income,medication type,self-assessed physical condition,fear of falling,knowledge and practices in fall prevention,social support,and fall risk.These factors collectively explained 34.3%of the variance in self-management behaviors.Conclusion:The findings highlight the need for targeted interventions to enhance fall prevention self-management among community-dwelling older adults.Interventions should focus on improving knowledge,strengthening social support,and addressing psychological barriers such as fear of falling.This study provides valuable insights for developing community-based strategies to reduce fall risks and promote healthy aging.
基金Supported by National Institutes of Health Grants,No.R01DK103055(to Ro S)RosVivo Therapeutics,No.AWD-01-00003158(to Ro S)the National Research Foundation of Korea Grant Funded by the Korean Government(MSIT),No.NRF-2021R1C1C2006743(to Kim MS)and No.NRF-2021R1A2C1095311(to Lee MY).
文摘BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of the gut.Loss of miR-10b-5p in ICC im-pairs intestinal motility and contributes to constipation,a common condition in the elderly.Notably,miR-10b-5p is co-expressed with its paralog,miR-10a-5p,in ICC.AIM To investigate the roles of miR-10a-5p and miR-10b-5p in age-associated intestinal dysmotility and assess the therapeutic potential of restoring their expression.METHODS We employed aged mice,mir-10a and mir-10b single and double knockout(KO)models,and human plasma and colon samples across age groups.GI and colonic transit,ICC network integrity,and expression levels of miR-10a/b-5p were eva-luated.Additionally,we tested whether treatment with their microRNA mimics could restore GI motility in aged mice.RESULTS Aged mice exhibited delayed GI and colonic transit,reduced fecal output,and diminished expression of miR-10a-5p and miR-10b-5p,which peaked during late embryonic and early postnatal stages and declined with age.This decline para-lleled ICC network deterioration in the colon.All KO models exhibited impaired motility and ICC loss,with mir-10a KO mice displaying more severe phenotypes than mir-10b KO mice.Double KO mice demonstrated growth retardation and reduced survival,with homozygous mutants living only up to 3 months.Treatment of aged mice with miR-10a-5p and miR-10b-5p mimics encapsu-lated in jetPEI significantly improved GI and colonic motility.Successful delivery to the gut,including the colon,was confirmed.In human samples,both miR-10a/b-5p and KIT expression decreased with age.CONCLUSION miR-10a-5p and miR-10b-5p are essential for ICC maintenance and colonic motility,and their age-related decline contributes to GI dysmotility in both mice and humans.Restoring their levels offers a promising therapeutic stra-tegy for treating age-related constipation and other motility disorders.
基金Supported by the High-level Talent Program of Yangzhou University to Yingying ZHANG。
文摘The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,potentially affecting their interactions with pollutants.This study aims to compare the combined toxicity effects of virgin and aged MFs with the common heavy metal copper(Cu).Gibel carp Carassius auratus gibelio were exposed to 1 mg/L of virgin and aged polyacrylonitrile MFs(PanMfs)separately and in combination with Cu(0.5-mg/L copper sulfate)for 21 d.Results show that both aged and virgin PanMfs significantly reduced Cu accumulation in gibel carp,with no notable difference.Cu adversely affected the carp's intestine and liver,while PanMfs alone had minimal histological impact but notably mitigated intestinal damage caused by Cu.Enzyme activity and gene expression analysis indicated that both PanMfs and Cu exposure,alone or combined,compromised gibel carp's antioxidant capacity,digestive and liver functions,induced inflammation,and affected liver metabolism and intestinal immune response.Integrated biomarker response analysis of enzyme activity and gene expression showed that combined Cu and PanMfs exposure had a stronger impact on gibel carp than individual exposure.However,whether exposed individually or in combination,aged PanMfs had a slightly greater impact than virgin PanMfs.This study showed that PanMfs alone exhibit minimal toxic effects on gibel carp but significantly modify Cu toxicity,with negligible differences between aged and virgin PanMfs except for slight disparities in enzyme activity and gene expression.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.52378432 and 52278426)the Key Research and Development Program of Shaanxi Province(Grant No.2022SF-169).
文摘The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of bio-oils and their component correspondence with petroleum asphalt were compared.The diffusion and fusion effects of various bio-oils in aged asphalt were introduced.Bio-oil cannot be used as a direct alternative of petroleum asphalt,but it has the potential to effectively rejuvenate aged asphalt binders due to the component similarity with petroleum asphalt and good diffusion properties.For the asphalt rejuvenation,the functionalization treatment methods of bio-oil were discussed such as purification,composition modification and component conversion.The active groups and derivatives in bio-oil can be converted into the missing components of the aged binder through phenolate,grafting,polycondensation,resinifying,but the conversion process and mechanism are still unclear.From the perspectives of diffusion behavior,components regulation,dissolving asphaltene and micro-rejuvenation effect,the rejuvenation behavior and mechanism of bio-oil on aged asphalt were elaborated,and the effects of various types of bio-oil and aged asphalt on rejuvenation behavior were analyzed.The preparation process and dosage of bio-rejuvenator were summarized.The rejuvenation effects of bio-oil on aged asphalt were comprehensively investigated from the aspects of high and low temperature performances,rheological properties,microstructure and chemical composition of bio-rejuvenated asphalt binders.Finally,the limitations of bio-oil used as asphalt rejuvenators were discussed,and future research directions were prospected,which can provide reference and theoretical basis for the development of high-performance bio-oil rejuvenating agents and the engineering application of bio-oil to improve the properties of aged asphalt materials.
基金Funded by the National Natural Science Foundation of China(Nos.52378444,52078130)the Natural Science Foundation of Shandong Province(No.ZR2021QE250)。
文摘The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack resistance of mixture.To address the issue,dry-method styrene-butadiene-styrene(DSBS)and epoxy resin were mixed with aged asphalt to prepare SBS-modified epoxy reclaimed asphalt(SERA).The micro fusion characteristics and mechanical properties of SERA were evaluated,and the optimal DSBS dosage was determined based on various tests.The results show that adding DSBS can enable the tensile toughness and low-temperature performance of SERA with less EP content to reach or exceed the performance level of epoxy reclaimed asphalt(ERA)with higher EP content.At 30%EP content,the recommended dry-method SBS content is 9%;At 40%EP content,the recommended dry-method SBS content is 5%;When the EP content is 50%,the recommended dry-method SBS content is 7%.
文摘Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yungang Grottoes,with their solemn Buddhas,stand as timeless sentinels,whispering tales of a millennium's passage.On the sacred slopes of Mount Wutai,the air is scented with incense,and the tranquil spirit of Zen envelops the misty landscape.Journey back to the heart of the Three Jins,where the aroma of aged vinegar lingers in the air,and hidden vistas reveal their profound beauty.Here,you will discover a charm and character like no other,as the spirit of the land unveils its unique poetic elegance and grace.
基金Supported by Hengyang Social Science Foundation Project,No.2024C027.
文摘Hengyang City faces challenges from rapid aging,including weakened family care and a shortage of resources.The"three social linkage"mechanism integrates community of practice,social organizations,and professional support to improve health services for the aged.However,issues like low smart device use and talent shortages persist.This study aims to optimize resource allocation and service efficiency through a"chain+virtual elderly care"model.This article explores the"three social linkage"mechanism and the integration of chain services with virtual elderly care models in Hengyang City.It examines three core elements:Resource integration,service collaboration,and professional support.The community coordinates resources and maintains databases of elderly care needs,while social organizations provide services and social workers design care plans.Data was gathered through case studies,interviews,and observations,focusing on smart technology integration and service outcomes.The study also evaluates crossdepartmental data sharing,social worker training,and funding mechanisms,using performance indicators like service delivery time,user satisfaction,and resource use.The analysis of elderly care services in Hengyang City reveals key issues and improvements within the"three social linkage"mechanism.The three social linkage mechanism has facilitated better resource integration and service collaboration.Communities have created resource lists and service demand databases,improving coordination.Social organizations provide specialized services,and social workers conduct home visits to create personalized care plans.The virtual elderly care platform has enhanced service efficiency,with smart devices like bracelets enabling real-time health monitoring.The"chain+virtual elderly care"model in Hengyang has effectively addressed issues of scattered and outdated resources by integrating services through community hubs,standardized stations,and dynamic resource databases.Smart elderly care platforms,especially devices like wristbands,have improved service efficiency and emergency response.
文摘Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducted from October to December 2022 using a convenience sampling method.A total of 312 older adults were selected from 13 day service centers for older adults in Macao,China.The Advance Care Planning Acceptance Questionnaire and the Family Adaptation,Partnership,Growth,Affection,Resolve(APGAR)Scale were used to survey the older adults.Results:A total of 306 older adults completed the survey.The score for advance care planning readiness was 65.55±10.69,and 59.5%of participants(n=182)were willing to participate in ACP.The family function score was 7.24±2.51,while 70.3%of participants were from a highly functional family.The higher family function indicating a higher readiness for advance care planning(r=0.396,P<0.001).The multiple linear regression analysis indicated that the variables“age,”“knowledge of ACP,”“experience with ACP,”and“received resuscitation of yourself,relatives or friends”combined with“family function”can influence advance care planning readiness among older adults(R^(2)=0.317,F=27.898,P<0.001).Conclusions:Older adults in Macao’s day service centers were willing to engage in ACP.The importance of family involvement is highlighted in the ACP readiness.Health education and improved family communication are vital for promoting ACP,which ensures individuals receive care when they lack the capacity to make that choice.Additionally,healthcare professionals should enhance communication and education with older adults during the medical care process.
基金supported in part by the National Key Basic Research and Development Program of China(2019YFA0801402,2018YFA0107200,2018YFA0801402,2018YFA0800100)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020501 and XDA16020404)the National Natural Science Foundation of China(32130030,31900454,32470866,32471010,32100800)。
文摘Alzheimer's disease(AD),the most prevalent form of dementia,disproportionately affects the elderly population.While aging is widely recognized as a major risk factor for AD,the precise mechanisms by which aging contributes to the pathogenesis of AD remain poorly understood.In our previous work,the neuropathological changes in the brains of aged cynomolgus monkeys(≥18 years old)following parenchymal cerebral injection of amyloid-β oligomers(AβOs)have been characterized.Here,we extend our investigation to middle-aged cynomolgus monkeys(≤15 years old)to establish an AD model.Surprisingly,immunohistochemical analysis reveals no detectable AD-related pathology in the brains of middle-aged monkeys,even after AβOs injection.In a comprehensive pathological analysis of 38 monkeys,we observe that the amyloid-β(Aβ)burden increases significantly with advancing age.Notably,the density of Aβ plaques is markedly higher in the ventral regions compared with the dorsal regions of aged monkey brains.Furthermore,we demonstrate that tau phosphorylation coincides with the accumulation of extensive Aβplaques and exhibits a positive correlation with Aβ burden in aged monkeys.Collectively,these findings underscore the critical role of the aged brain in providing the necessary conditions for AβO-induced AD pathologies in cynomolgus monkeys.
基金the National Natural Science Foundation of China(82172266to P.G.W.)Natural Science Foundation of Beijing(7232002 to N.G.).
文摘The Japanese encephalitis virus(JEV)causes Japanese encephalitis(JE),a severe disease that primarily affects children and induces significant central nervous system complications.With the widespread adoption of vaccination in children,the incidence among older individuals has increased substantially.Despite this epidemiological shift,research on JEV infection in the elderly remains limited.We established JEV infection models using both aged and young mice to explore age-related differences in pathology and underlying mechanisms.Brain tissue samples were analyzed for pathological changes and viral tropism in major cell types.To further characterize immune response variations,we conducted transcriptomic sequencing on the brain tissues following JEV infection.Aged mice exhibited lower mortality,delayed disease progression,and milder brain pathology compared to young mice after JEV infection.Viral titers and infection rates of major brain cell types were similar in both groups.Transcriptomic analysis revealed diminished immune activation and weaker inflammatory responses in aged mice.Additionally,microglial activation and CD8^(+) T cell function were significantly reduced.Interestingly,JEV infection induced the selective recruitment of B cells in the brains of aged mice.These B cells may modulate the effects of CD8^(+) T cells in the disease process.Compared to young mice,aged mice showed enhanced resistance to JEV progression and reduced brain pathology.This resistance was associated with a weakened immune response in the aged brain,rather than differences in viral infection.The specific recruitment of B cells in the brains of aged mice may play a crucial role in limiting disease progression.
文摘There is no way around it.Regular physical activity(PA)and a high-quality diet are essential to promoting good health and prolonging life in middle-aged and older adults.In the November 2024 Issue of the Journal of Sport and Health Science,Ding et al.
基金supported by the Shanghai New Three-year Action Plan for Public Health(Grant No.GWV-10.1-XK16)the US National Institute on Aging(RO1-AGO34479).
文摘In 2019,China had over 13.14 million dementia cases,with incidence rates of(56.47–207.08)/100,000[1].Early cognitive impairment—a key dementia symptom—reduces quality of life,increases care dependence,and lowers survival in older adults[2].A decline in physical function can also be observed in older adults with increasing age.Grip strength has been shown to be a marker of overall physiological function in older adults.
基金supported by Peking University,the National Natural Science Foundation of China,the National Institute on Aging and the World Bank。
文摘Background It remains unclear whether sleep duration and physical activity(PA)trajectories in middle-aged and older adults are associated with different risks of cardiovascular diseases(CVDs).This study aimed to explore the trajectories of total sleep duration and PA among middle-aged and older Chinese adults and their impact on CVD risk.Methods This study was based on the China Health and Retirement Longitudinal Study.12009 adults aged 45 years and older from five waves were included.CVD events were measured by self-reports of heart disease and stroke.We first used groupbased trajectory modeling to identify total sleep duration and PA trajectories from 2011 to 2020,and then employed logistic regression models to analyze their risk for CVD.Results We identified three sleep duration and PA trajectories.The risk of heart disease increased by 33%(OR=1.31,95%CI:1.12-1.53)for the short sleep duration trajectory(vs.moderate sleep duration trajectory),by 40%(OR=1.40,95%CI:1.06-1.84)for the high decreasing PA trajectory,and by 20%(OR=1.20,95%CI:1.01-1.42)for the low stable PA trajectory(vs.high stable PA trajectory),respectively.Similar results for stroke and CVD as the outcomes were also observed,but the higher risk of stroke in the high decreasing PA trajectory group was not statistically significant.The joint effects of sleep and PA showed lower risks of heart disease and stroke in trajectories with moderate or long sleep duration and high stable PA compared with short sleep duration and a low stable PA trajectory.Conclusions Short total sleep duration,high decreasing PA,and low stable PA trajectories could increase the risk of CVDs among middle-aged and older adults.Long-term moderate to long total sleep durations and high stable PA trajectories might be optimal for preventing CVDs.
