Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)ha...Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)has been proven to enhance antioxidant capacity,regulate lipid metabolism and gut microbiota in mammals,but its efficacy in hens remains unclear.Hence,this study aimed to investigate whether dietary GL supplementation improves reproductive performance in hens during the late laying stage by modulating intestinal microbiota composition,hepatic lipid metabolism and ovarian antioxidant status.Results Dietary supplementation with 100 mg/kg GL significantly improved the egg production rate,egg quality,and hatching rate in aged breeder hens(P<0.05).GL supplementation also increased the serum levels of HDLC,TP and ALB,and enhanced the antioxidant capacity in both serum and ovary(P<0.05).In addition,dietary GL elevated the serum progesterone(P4)levels by enhancing the transcription level of steroid synthesis key enzymes(CYP11A1 and 3β-HSD)in the ovary(P<0.05).Dietary GL also promoted the synthesis and transport of vitellogenin(VTG)by upregulating the VTG-Ⅱ(P<0.05)and APOV1(P=0.077)expression levels in the liver,thereby increasing the number of grade follicles and small yellow follicles.Moreover,dietary GL enhanced hepatic fatty acidβ-oxidation by upregulating PPARαand CPT-I(P<0.05),and downregulating ACC expression levels(P<0.05).In agreement,liver metabolomics analysis revealed that dietary GL supplementation significantly altered hepatic metabolism,with 389 differentially identified metabolites(P<0.05).The key metabolites(e.g.,taurocholic acid,tauroursodeoxycholic acid,nicotinuric acid,glycodeoxycholic acid(hydrate))were identified,and they were mainly functionally enriched in betaalanine metabolism nicotinate,taurine and hypotaurine metabolism(P<0.05).Finally,16S rRNA gene sequencing revealed that dietary GL reversed age-induced changes in gut microbiota composition,characterized by a significant increase in Lactobacillus abundance and a decrease in Bacteroides(P<0.05).Conclusions These results collectively demonstrate that dietary supplementation with 100 mg/kg GL improved reproductive performance by reversing age-induced changes in gut microbiota,enhancing hepatic vitellogenin synthesis,and ameliorating ovarian function in aged breeder hens.This study suggests that dietary GL is a potential strategy to improve reproductive performance in broiler breeder hens during the late laying period.展开更多
Understanding how aging influences the thermal hazards of lithium-ion batteries(LIBs)is critical for enhancing their safety across a wide range of applications.This study systematically investigates the thermal runawa...Understanding how aging influences the thermal hazards of lithium-ion batteries(LIBs)is critical for enhancing their safety across a wide range of applications.This study systematically investigates the thermal runaway(TR)behavior of LIBs,with particular emphasis on combined-pathway aging,evaluated in terms of normalized usable capacity(U_(E)).Key thermal safety parameters,i.e.,TR triggering temperature,mass loss,and heat generation under diverse aging conditions,are quantified.To enable a fair comparison,thermal hazards are evaluated based on equivalent usable capacity,revealing that aged cells exhibit lower TR triggering temperatures and higher heat generation than fresh cells under thermal abuse with elevated thermal risks.Mechanistic analysis identifies lithium plating,solid electrolyte interphase(SEI)formation,and lithium depletion,particularly under high-temperature charging,as the dominant contributors to increased hazard.Using an aging-stressor matrix,a trade-off between high-C-rateinduced thermal instability and high-temperature-induced thermal stability is discovered and quantified,underscoring the strong dependence of thermal hazards on specific aging pathways.This work advances the fundamental understanding of aging-induced safety risks in LIBs and offers practical guidance for the development of safer battery systems,optimized charging protocols,and improved battery management strategies across applications in electric vehicles,consumer electronics,and grid-scale energy storage.展开更多
Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major coh...Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major cohort for stem cell-based therapies.However,the regenerative potential of stem cells significantly decreases with advanced age and deteriorating health status of the donor.Therefore,the efficacy of autologous stem cell therapy is significantly compromised in older patients.To overcome these limitations,alternative strategies have been used to restore the age-and disease-depleted function of stem cells.These methods aim to restore the therapeutic efficacy of aged stem cells for autologous use.This article explores the effect of donor age and health status on the regenerative potential of stem cells.It further highlights the limitations of stem cell-based therapy for autologous treatment in the elderly.A comprehensive insight into the potential strategies to address the“age”and“disease”compromised regenerative potential of autologous stem cells is also presented.The information provided here serves as a valuable resource for physicians and patients for optimization of stem cellbased autologous therapy for aged patients.展开更多
Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of n...Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of neurodegenerative diseases with glaucoma being highly prevalent.Glaucoma is characterized by the progressive dysfunction and loss of retinal ganglion cells and their axons which make up the optic nerve.It is the leading cause of irreversible vision loss and affects an estimated 80 million people.The mammalian central nervous system is non-regenerative and,once lost or injured,retinal ganglion cells cannot regenerate an axon into the optic nerve under basal conditions.Thus,strategies that provide neuroprotection to stressed,dysfunctional,or dying retinal ganglion cells are likely to be of high therapeutic and translational value.Advancing age,genetics,and elevated intraocular pressure are all major risk factors for glaucoma,however,all clinically available glaucoma treatments focus on intraocular pressure management and do not directly address the neurodegenerative component of glaucoma.展开更多
Delayed neurocognitive recovery following anesthesia and surgery is a common complication in older adult patients.Synapses are fundamental to cognitive function.The activity of synapses heavily depends on the energy s...Delayed neurocognitive recovery following anesthesia and surgery is a common complication in older adult patients.Synapses are fundamental to cognitive function.The activity of synapses heavily depends on the energy supplied by synaptic mitochondria,which are significantly influenced by oxidative stress.Sirtuin 3 is a histone deacetylase located in the mitochondrial matrix that plays a pivotal role in regulating mitochondrial function.However,it remains unclear whether and how sirtuin 3 is involved in the development of delayed cognitive recovery.Therefore,in this study,we investigated the potential role of sirtuin 3 in synapses during delayed neurocognitive recovery.Our results showed that anesthesia and surgery induced cognitive impairment in mice and reduced sirtuin 3 protein expression.Overexpression of sirtuin 3 inhibited opening of the mitochondrial permeability transition pore by reducing acetylation of K166 on cyclophilin D and also rescued cognitive impairment.Aged mice carrying the cyclophilin D-K166R mutation exhibited significantly reduced cognitive impairment.Similarly,administering the mitochondrial permeability transition pore blocker,cyclosporine A,effectively alleviated the decline in synaptic mitochondrial function and cognitive impairment caused by anesthesia and surgery in aged mice.These results indicate that the sirtuin 3/cyclophilin D-K166/mPTP signaling pathway in hippocampal synaptic mitochondria is involved in delayed neurocognitive recovery of aged mice,suggesting this pathway could serve as a potential target for treatment.展开更多
BACKGROUND Solid fuel use for cooking and heating is a major environmental risk factor,yet its association with new-onset heart disease(HD)remains unclear.The aim of this study was to investigate the relationship betw...BACKGROUND Solid fuel use for cooking and heating is a major environmental risk factor,yet its association with new-onset heart disease(HD)remains unclear.The aim of this study was to investigate the relationship between solid fuel exposure and new-onset HD in a large cohort.METHODS Multivariable logistic regression models assessed associations between cooking/heating fuel types(coal,crop residue/wood,liquefied petroleum gas,natural gas,and others)and new-onset HD.Subgroup analyses explored effect modification by age,sex,education,smoking,alcohol use,and region.RESULTS A prospective cohort study included 5915 participants,with 781 participants(13.2%)developing new-onset HD.Coal use for cooking showed an initial association with new-onset HD risk(OR=1.41,95%CI:1.06–1.86,P=0.02),which attenuated after full adjustment(OR=1.28,95%CI:0.96–1.72,P=0.10).Coal use for heating demonstrated robust associations across all models(OR=1.86,95%CI:1.42–2.43,P<0.001).Crop residue/wood burning for heating was also significant(Model 2:OR=1.40,95%CI:1.06–1.86,P=0.02).Subgroup analyses revealed stronger associations among females,non-smokers,non-drinkers,and less-educated participants.Geographic stratification showed significant associations in southern but not northern regions.CONCLUSIONS Solid fuel use,particularly coal for heating,is associated with increased new-onset HD risk.Reducing solid fuel exposure is crucial for HD prevention in low-resource settings.展开更多
AIM:To evaluate the visual function and optical quality in adults aged 80y and older following the bilateral implantation of zonal refractive multifocal intraocular lens(IOL,LS-313 MF30).METHODS:A single-center,non-ra...AIM:To evaluate the visual function and optical quality in adults aged 80y and older following the bilateral implantation of zonal refractive multifocal intraocular lens(IOL,LS-313 MF30).METHODS:A single-center,non-randomized,prospective clinical trial was conducted,involving cataract patients aged 80 y and older.Patients received bilateral implantation of the LS-313 MF30 or CT Asphina 409MP,based on personal preference.Postoperative assessments included uncorrected and corrected visual acuity at distance,intermediate,and near ranges,as well as defocus curve.Subjective evaluations were performed using the visual function(VF-14)questionnaire,spectacle independence rates,and patient satisfaction surveys.Photic phenomena such as glare,halos,and starbursts were also analyzed.RESULTS:The MF30 group(16 eyes from 8 participants,85.38±2.56 y)exhibited superior uncorrected and corrected intermediate and near visual acuity compared to the 409MP group(26 eyes from 13 participants,85.77±2.20 y),while distance visual acuity was comparable between groups.The defocus curve of the MF30 group revealed two peaks at 0.00 D and-3.00 D,indicating a broader depth of focus.Patients in the MF30 group reported higher rates of spectacle independence and greater satisfaction.While photic phenomena such as glare(28.6%vs 18.5%,P=0.584),starburst(9.5%vs 3.7%,P=0.567)and halos(23.8%vs 11.11%,P=0.438)were more prevalent in the MF30 group,they were generally mild and did not significantly impact daily activities.CONCLUSION:Zonal refractive multifocal IOLs provide elderly patients with improved distance and near vision,greater spectacle independence,and greater satisfaction.Although photic phenomena were slightly more frequent with MF30,they are generally reported as non-disruptive and do not affect their daily life compared to monofocal IOLs.展开更多
Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hep...Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hepatic ApoE expression(ΔApoE),24-month-oldΔApoE mice displayed a 95%reduction in circulating ApoE levels and significantly improved fracture healing.ApoE treatment of aged BMSCs inhibited osteoblast differentiation in tissue culture models;RNA-seq,Western blot,immunofluorescence,and RT-PCR analyses indicated that the Wnt/β-catenin pathway is the target of this inhibition.Indeed,we showed that ApoE had no effect on cultures with stabilizedβ-catenin levels.Next,we determined that Lrp4 serves as the osteoblast cell surface receptor to ApoE,as expression of Lrp4 is required in ApoE-based inhibition of Wnt/β-catenin signaling and osteoblast differentiation.Importantly,we validated this ApoE-Lrp4-Wnt/β-catenin molecular mechanism in human osteoblast differentiation.Finally,we identified an ApoE-neutralizing antibody(NAb)and used it to treat aged,wildtype mice 3 days after fracture surgery resulting in fracture calluses with 35%more bone deposition.Our work here identifies novel liver-to-bone cross-talk and a noninvasive,translatable therapeutic intervention for aged bone regeneration.展开更多
Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducte...Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducted from October to December 2022 using a convenience sampling method.A total of 312 older adults were selected from 13 day service centers for older adults in Macao,China.The Advance Care Planning Acceptance Questionnaire and the Family Adaptation,Partnership,Growth,Affection,Resolve(APGAR)Scale were used to survey the older adults.Results:A total of 306 older adults completed the survey.The score for advance care planning readiness was 65.55±10.69,and 59.5%of participants(n=182)were willing to participate in ACP.The family function score was 7.24±2.51,while 70.3%of participants were from a highly functional family.The higher family function indicating a higher readiness for advance care planning(r=0.396,P<0.001).The multiple linear regression analysis indicated that the variables“age,”“knowledge of ACP,”“experience with ACP,”and“received resuscitation of yourself,relatives or friends”combined with“family function”can influence advance care planning readiness among older adults(R^(2)=0.317,F=27.898,P<0.001).Conclusions:Older adults in Macao’s day service centers were willing to engage in ACP.The importance of family involvement is highlighted in the ACP readiness.Health education and improved family communication are vital for promoting ACP,which ensures individuals receive care when they lack the capacity to make that choice.Additionally,healthcare professionals should enhance communication and education with older adults during the medical care process.展开更多
Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived mi...Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived miRNAs may play a key role in modulating embryonic oxidation-reduction process,whereas the exact function and mechanism were still poorly understood.The present study aimed to investigate the roles of egg exosome miRNAs in maintaining dynamic equilibrium of free radicals and peroxide agents in embryonic liver,as well as demonstrate the specific mechanism using oxidative stress-challenged hepatocytes.Results Compared to 36-week-old breeding hens,decreased hatchability and increased embryonic mortality were observed in 65-week-old breeding hens.Meanwhile,the older group showed the increased MDA levels and decreased SOD and GSH-Px activities in embryonic liver,muscle and serum.Embryonic mortality was significantly positively correlated with MDA level and negatively correlated with GSH-Px activity in embryonic liver.In addition,363 differentially expressed genes(DEGs)were identified in embryonic liver,13 differentially expressed miRNAs(DE-miRNAs)were identified in egg exosomes.These DEGs and DE-miRNAs were involved in oxidoreductase activity,glutathione metabolic process,MAPK signaling pathway,apoptosis and autophagy.miRNA-mRNA network analysis further found that DEGs targeted by DE-miRNAs were mainly enriched in programmed cell death,such as apoptosis and autophagy.Wherein,MAPK10 with highest MCC and AUC values was significantly related to GSH-Px activity and MDA level,and served as the target gene of miR-145-5p based on dual luciferase reporter experiment and correlation analysis.Bioinformatics analysis found that miR-145-5p/MAPK10 axis might alleviate peroxide generation and apoptosis.In primary hepatocytes of chick embryos,miR-145-5p transfection significantly reversed H_(2)O_(2)-induced mitochondrial ROS increase,MAPK10,BAX and CASP3 overexpression and excessive apoptosis.Conclusion Exosome miR-145-5p in eggs could target MAPK10 and decrease mitochondrial ROS,attenuating oxidative damage and apoptosis in hepatocytes of chick embryos.These findings may provide new theoretical basis for the improvement of maternal physiological status to maintain embryonic redox homeostasis by nutritional or genetic modifications.展开更多
Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted ...Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted on problems in middle-aged people have focused on visual disorders without taking into account all the ocular morbidities that may affect this segment of the population, hence the present study, the aim of which is to determine the proportions of different eye diseases in people aged 40 and over. Materials and Methods: This was a descriptive cross-sectional study carried out in the ophthalmology department covering the period from January 1 to December 31, 2020. Results: In total, we collected 828 patients aged 40 and over out of 1811 patients who received ophthalmological consultation during the study period, representing 45.72%. The most represented age group was 40 - 50 years, with an average age of 58.84 years and a maximum of 93 years. There were slightly more women (62.3%) than men (37.7%). The main reasons for consultation were decreased visual acuity (26.4%) and pruritus (19.9%). The main eye diseases diagnosed were cataracts (23%), allergic conjunctivitis (21.1%), and bacterial conjunctivitis (14.2%). Discussions: The predominance of cataracts in the diagnosed diseases confirms the literature data, according to which the main eye morbidities in middle-aged and elderly people are cataracts, glaucoma, and age-related macular degeneration. Conclusion: It is crucial to have a mastery of these epidemiological data of eye diseases in order to adapt the technical platforms of eye care structures to the needs of different segments of the population.展开更多
Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mR...Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mRNA transcriptomics was used to analyze differentially expressed genes and ferroptosis markers.Next,a weighted correlation network analysis was conducted on these genes.Then,common genes among ferroptosis,Yinyanghuo,and differentially expressed genes were identified as target genes.Single-cell and spatial transcriptomics were analyzed to evaluate expression changes of target genes in bone marrow.Furthermore,to validate the results of bioinformatics analysis,MC3T3-E1 cells were used to model the preventive effects of icariin in vitro,and ferroptosis markers and mitochondrial function were both examined.In vivo,4-month-old mice were fed a diet containing icariin for 14 months,following which bone proteomics was assessed to identify essential proteins.Hematoxylin-eosin staining and immunohistochemistry assays were performed on mouse femurs.Finally,Western blot and polymerase chain reaction(PCR)analyses were performed to analyze the effects of icariin on ferroptosis target biomarkers.Ptgs2 and Hmox1 were identified as target genes related to both icariin and ferroptosis.The expression of ferroptosis-related genes was up-regulated with age.Moreover,single-cell and spatial transcriptomics analyses revealed that up-regulation of these two genes inhibited osteogenic capability.The results of the in vitro experiments indicated that icariin mitigated the accumulation of reactive oxygen species and β-galactosidase.Similarly,icariin prevented aberrant changes in the levels of ferroptosis-related proteins,consistent with the results of the in vivo experiments.Specifically,10 mg/(kg·day)of icariin inhibited ferroptosis and osteoporosis in aged mice.Overall,this study revealed that icariin could serve as a dietary supplement to prevent age-induced osteoporosis.Furthermore,Ptgs2 and Hmox1 were identified as ferroptosis-related targets through which icariin exerts its protective effects.展开更多
Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.H...Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.However,the status and influencing factors of these behaviors among community-dwelling older adults in China remain poorly understood.This study aimed to investigate fall prevention self-management behaviors and their influencing factors among community-dwelling older adults in Fuzhou,China.Methods:From June to December 2022,we used multi-stage cluster random sampling and invited 675 community-dwelling older adults from Fujian as participants.We conducted a questionnaire survey using the general information questionnaire,Fall Prevention Self-management Behavior among Older Adults Questionnaire,Questionnaire on Knowledge,Belief,and Practice of Falls Prevention among Older Adults in Community,Modified Falls Efficacy Scale,Social Support Rating Scale,and Chinese Version of the Self-Assessment Fall Risk Scale for Older Adults.Multivariate linear regression was used to identify the factors influencing fall prevention self-management behavior among older adults.Results:The overall score of fall prevention self-management behaviors was 146.81±34.22,indicating a moderate level.Key influencing factors included education level,pre-retirement occupation,income,medication type,self-assessed physical condition,fear of falling,knowledge and practices in fall prevention,social support,and fall risk.These factors collectively explained 34.3%of the variance in self-management behaviors.Conclusion:The findings highlight the need for targeted interventions to enhance fall prevention self-management among community-dwelling older adults.Interventions should focus on improving knowledge,strengthening social support,and addressing psychological barriers such as fear of falling.This study provides valuable insights for developing community-based strategies to reduce fall risks and promote healthy aging.展开更多
BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of t...BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of the gut.Loss of miR-10b-5p in ICC im-pairs intestinal motility and contributes to constipation,a common condition in the elderly.Notably,miR-10b-5p is co-expressed with its paralog,miR-10a-5p,in ICC.AIM To investigate the roles of miR-10a-5p and miR-10b-5p in age-associated intestinal dysmotility and assess the therapeutic potential of restoring their expression.METHODS We employed aged mice,mir-10a and mir-10b single and double knockout(KO)models,and human plasma and colon samples across age groups.GI and colonic transit,ICC network integrity,and expression levels of miR-10a/b-5p were eva-luated.Additionally,we tested whether treatment with their microRNA mimics could restore GI motility in aged mice.RESULTS Aged mice exhibited delayed GI and colonic transit,reduced fecal output,and diminished expression of miR-10a-5p and miR-10b-5p,which peaked during late embryonic and early postnatal stages and declined with age.This decline para-lleled ICC network deterioration in the colon.All KO models exhibited impaired motility and ICC loss,with mir-10a KO mice displaying more severe phenotypes than mir-10b KO mice.Double KO mice demonstrated growth retardation and reduced survival,with homozygous mutants living only up to 3 months.Treatment of aged mice with miR-10a-5p and miR-10b-5p mimics encapsu-lated in jetPEI significantly improved GI and colonic motility.Successful delivery to the gut,including the colon,was confirmed.In human samples,both miR-10a/b-5p and KIT expression decreased with age.CONCLUSION miR-10a-5p and miR-10b-5p are essential for ICC maintenance and colonic motility,and their age-related decline contributes to GI dysmotility in both mice and humans.Restoring their levels offers a promising therapeutic stra-tegy for treating age-related constipation and other motility disorders.展开更多
The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of b...The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of bio-oils and their component correspondence with petroleum asphalt were compared.The diffusion and fusion effects of various bio-oils in aged asphalt were introduced.Bio-oil cannot be used as a direct alternative of petroleum asphalt,but it has the potential to effectively rejuvenate aged asphalt binders due to the component similarity with petroleum asphalt and good diffusion properties.For the asphalt rejuvenation,the functionalization treatment methods of bio-oil were discussed such as purification,composition modification and component conversion.The active groups and derivatives in bio-oil can be converted into the missing components of the aged binder through phenolate,grafting,polycondensation,resinifying,but the conversion process and mechanism are still unclear.From the perspectives of diffusion behavior,components regulation,dissolving asphaltene and micro-rejuvenation effect,the rejuvenation behavior and mechanism of bio-oil on aged asphalt were elaborated,and the effects of various types of bio-oil and aged asphalt on rejuvenation behavior were analyzed.The preparation process and dosage of bio-rejuvenator were summarized.The rejuvenation effects of bio-oil on aged asphalt were comprehensively investigated from the aspects of high and low temperature performances,rheological properties,microstructure and chemical composition of bio-rejuvenated asphalt binders.Finally,the limitations of bio-oil used as asphalt rejuvenators were discussed,and future research directions were prospected,which can provide reference and theoretical basis for the development of high-performance bio-oil rejuvenating agents and the engineering application of bio-oil to improve the properties of aged asphalt materials.展开更多
The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,p...The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,potentially affecting their interactions with pollutants.This study aims to compare the combined toxicity effects of virgin and aged MFs with the common heavy metal copper(Cu).Gibel carp Carassius auratus gibelio were exposed to 1 mg/L of virgin and aged polyacrylonitrile MFs(PanMfs)separately and in combination with Cu(0.5-mg/L copper sulfate)for 21 d.Results show that both aged and virgin PanMfs significantly reduced Cu accumulation in gibel carp,with no notable difference.Cu adversely affected the carp's intestine and liver,while PanMfs alone had minimal histological impact but notably mitigated intestinal damage caused by Cu.Enzyme activity and gene expression analysis indicated that both PanMfs and Cu exposure,alone or combined,compromised gibel carp's antioxidant capacity,digestive and liver functions,induced inflammation,and affected liver metabolism and intestinal immune response.Integrated biomarker response analysis of enzyme activity and gene expression showed that combined Cu and PanMfs exposure had a stronger impact on gibel carp than individual exposure.However,whether exposed individually or in combination,aged PanMfs had a slightly greater impact than virgin PanMfs.This study showed that PanMfs alone exhibit minimal toxic effects on gibel carp but significantly modify Cu toxicity,with negligible differences between aged and virgin PanMfs except for slight disparities in enzyme activity and gene expression.展开更多
The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack r...The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack resistance of mixture.To address the issue,dry-method styrene-butadiene-styrene(DSBS)and epoxy resin were mixed with aged asphalt to prepare SBS-modified epoxy reclaimed asphalt(SERA).The micro fusion characteristics and mechanical properties of SERA were evaluated,and the optimal DSBS dosage was determined based on various tests.The results show that adding DSBS can enable the tensile toughness and low-temperature performance of SERA with less EP content to reach or exceed the performance level of epoxy reclaimed asphalt(ERA)with higher EP content.At 30%EP content,the recommended dry-method SBS content is 9%;At 40%EP content,the recommended dry-method SBS content is 5%;When the EP content is 50%,the recommended dry-method SBS content is 7%.展开更多
Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yunga...Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yungang Grottoes,with their solemn Buddhas,stand as timeless sentinels,whispering tales of a millennium's passage.On the sacred slopes of Mount Wutai,the air is scented with incense,and the tranquil spirit of Zen envelops the misty landscape.Journey back to the heart of the Three Jins,where the aroma of aged vinegar lingers in the air,and hidden vistas reveal their profound beauty.Here,you will discover a charm and character like no other,as the spirit of the land unveils its unique poetic elegance and grace.展开更多
Objectives:This prospective cohort study examined the change trajectories of cognitive and physical functions of individuals with motoric cognitive risk(MCR)syndrome,as well as the longitudinal associations between MC...Objectives:This prospective cohort study examined the change trajectories of cognitive and physical functions of individuals with motoric cognitive risk(MCR)syndrome,as well as the longitudinal associations between MCR syndrome and changes in cognitive and physical functions,to provide a new perspective on preventing dementia.Methods:Participants were selected from the China Health and Retirement Longitudinal Study(CHARLS).Demographic characteristics,health status,and lifestyle variables were assessed in 2011.MCR syndrome was defined as the presence of subjective cognitive complaints and objective slow gait,with preserved activities of daily living and absence of dementia,and assessed in 2011.Cognitive function,including orientation,attention and calculation,episodic memory,and visuospatial ability,was measured from 2011 to 2018.Physical function,including grip strength,balance ability,and repeated chair stand tests,was measured from 2011 to 2015.Generalized estimating equation was employed to analyze the longitudinal associations between MCR syndrome in 2011 and changes in cognitive functions over 7 years and physical functions over 4 years.Results:Among 4,217 participants,475 had MCR syndrome in 2011.Both participants with MCR syndrome and those without exhibited a decline in both cognitive and physical function over 7 years and 4 years of follow-up,except for fluctuations in visuospatial ability.Non-MCR syndrome participants demonstrated significantly better overall cognitive function in 2018 compared to 2011(Group×Time:B=0.44,P=0.035)than those in the MCR syndrome group.However,participants with non-MCR syndrome demonstrated significantly worse visuospatial ability in 2013(Group×time:B=−0.44,P=0.002)and 2018(Group×time:B=−0.34,P=0.016)compared to those in the MCR syndrome group.Non-MCR syndrome participants demonstrated significantly better performance in repeated chair stand tests in 2013(Group×time:B=0.31,P<0.001)and 2015(Group×time:B=0.37,P<0.001)compared to those in the MCR syndrome group in 2011.Conclusions:Older adults with MCR syndrome experience worse overall cognitive and physical function performance,especially in repeated chair stand tests,than individuals without MCR syndrome over 7-year and 4-year follow-up periods.It is suggested that future interventional studies will target both physical and cognitive functions in MCR syndrome individuals,providing insights for the prevention of dementia.展开更多
基金supported and funded by the National Key Research and Development Program of China(2023YFD1300801)the Agricultural Science and Technology Innovation Program in Chinese Academy of Agricultural Sciences(ASTIP-IAS-08)。
文摘Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)has been proven to enhance antioxidant capacity,regulate lipid metabolism and gut microbiota in mammals,but its efficacy in hens remains unclear.Hence,this study aimed to investigate whether dietary GL supplementation improves reproductive performance in hens during the late laying stage by modulating intestinal microbiota composition,hepatic lipid metabolism and ovarian antioxidant status.Results Dietary supplementation with 100 mg/kg GL significantly improved the egg production rate,egg quality,and hatching rate in aged breeder hens(P<0.05).GL supplementation also increased the serum levels of HDLC,TP and ALB,and enhanced the antioxidant capacity in both serum and ovary(P<0.05).In addition,dietary GL elevated the serum progesterone(P4)levels by enhancing the transcription level of steroid synthesis key enzymes(CYP11A1 and 3β-HSD)in the ovary(P<0.05).Dietary GL also promoted the synthesis and transport of vitellogenin(VTG)by upregulating the VTG-Ⅱ(P<0.05)and APOV1(P=0.077)expression levels in the liver,thereby increasing the number of grade follicles and small yellow follicles.Moreover,dietary GL enhanced hepatic fatty acidβ-oxidation by upregulating PPARαand CPT-I(P<0.05),and downregulating ACC expression levels(P<0.05).In agreement,liver metabolomics analysis revealed that dietary GL supplementation significantly altered hepatic metabolism,with 389 differentially identified metabolites(P<0.05).The key metabolites(e.g.,taurocholic acid,tauroursodeoxycholic acid,nicotinuric acid,glycodeoxycholic acid(hydrate))were identified,and they were mainly functionally enriched in betaalanine metabolism nicotinate,taurine and hypotaurine metabolism(P<0.05).Finally,16S rRNA gene sequencing revealed that dietary GL reversed age-induced changes in gut microbiota composition,characterized by a significant increase in Lactobacillus abundance and a decrease in Bacteroides(P<0.05).Conclusions These results collectively demonstrate that dietary supplementation with 100 mg/kg GL improved reproductive performance by reversing age-induced changes in gut microbiota,enhancing hepatic vitellogenin synthesis,and ameliorating ovarian function in aged breeder hens.This study suggests that dietary GL is a potential strategy to improve reproductive performance in broiler breeder hens during the late laying period.
文摘Understanding how aging influences the thermal hazards of lithium-ion batteries(LIBs)is critical for enhancing their safety across a wide range of applications.This study systematically investigates the thermal runaway(TR)behavior of LIBs,with particular emphasis on combined-pathway aging,evaluated in terms of normalized usable capacity(U_(E)).Key thermal safety parameters,i.e.,TR triggering temperature,mass loss,and heat generation under diverse aging conditions,are quantified.To enable a fair comparison,thermal hazards are evaluated based on equivalent usable capacity,revealing that aged cells exhibit lower TR triggering temperatures and higher heat generation than fresh cells under thermal abuse with elevated thermal risks.Mechanistic analysis identifies lithium plating,solid electrolyte interphase(SEI)formation,and lithium depletion,particularly under high-temperature charging,as the dominant contributors to increased hazard.Using an aging-stressor matrix,a trade-off between high-C-rateinduced thermal instability and high-temperature-induced thermal stability is discovered and quantified,underscoring the strong dependence of thermal hazards on specific aging pathways.This work advances the fundamental understanding of aging-induced safety risks in LIBs and offers practical guidance for the development of safer battery systems,optimized charging protocols,and improved battery management strategies across applications in electric vehicles,consumer electronics,and grid-scale energy storage.
文摘Regenerative medicine is a promising therapeutic avenue for previously incurable diseases.As the risk of chronic and degenerative diseases significantly increases with age,the elderly population represents a major cohort for stem cell-based therapies.However,the regenerative potential of stem cells significantly decreases with advanced age and deteriorating health status of the donor.Therefore,the efficacy of autologous stem cell therapy is significantly compromised in older patients.To overcome these limitations,alternative strategies have been used to restore the age-and disease-depleted function of stem cells.These methods aim to restore the therapeutic efficacy of aged stem cells for autologous use.This article explores the effect of donor age and health status on the regenerative potential of stem cells.It further highlights the limitations of stem cell-based therapy for autologous treatment in the elderly.A comprehensive insight into the potential strategies to address the“age”and“disease”compromised regenerative potential of autologous stem cells is also presented.The information provided here serves as a valuable resource for physicians and patients for optimization of stem cellbased autologous therapy for aged patients.
基金supported by St.Erik Eye Hospital philanthropic donations,Vetenskapsrådet 2022-00799(to PAW).
文摘Neurodegenerative diseases account for a large and increasing health and economic burden worldwide.With an increasingly aged population,this burden is set to increase.Optic neuropathies make up a large proportion of neurodegenerative diseases with glaucoma being highly prevalent.Glaucoma is characterized by the progressive dysfunction and loss of retinal ganglion cells and their axons which make up the optic nerve.It is the leading cause of irreversible vision loss and affects an estimated 80 million people.The mammalian central nervous system is non-regenerative and,once lost or injured,retinal ganglion cells cannot regenerate an axon into the optic nerve under basal conditions.Thus,strategies that provide neuroprotection to stressed,dysfunctional,or dying retinal ganglion cells are likely to be of high therapeutic and translational value.Advancing age,genetics,and elevated intraocular pressure are all major risk factors for glaucoma,however,all clinically available glaucoma treatments focus on intraocular pressure management and do not directly address the neurodegenerative component of glaucoma.
基金supported by the National Natural Science Foundation of China,Nos.81701040(to HM),82071180(to HM),82271206(to TL),82171191(to YW),82371211(to YW)the Natural Science Foundation of Beijing,No.7212023(to HM)Key Subject of the Natural Science Foundation ofJiangsu Province for Colleges and Universities,No.23KJA320009(to YW).
文摘Delayed neurocognitive recovery following anesthesia and surgery is a common complication in older adult patients.Synapses are fundamental to cognitive function.The activity of synapses heavily depends on the energy supplied by synaptic mitochondria,which are significantly influenced by oxidative stress.Sirtuin 3 is a histone deacetylase located in the mitochondrial matrix that plays a pivotal role in regulating mitochondrial function.However,it remains unclear whether and how sirtuin 3 is involved in the development of delayed cognitive recovery.Therefore,in this study,we investigated the potential role of sirtuin 3 in synapses during delayed neurocognitive recovery.Our results showed that anesthesia and surgery induced cognitive impairment in mice and reduced sirtuin 3 protein expression.Overexpression of sirtuin 3 inhibited opening of the mitochondrial permeability transition pore by reducing acetylation of K166 on cyclophilin D and also rescued cognitive impairment.Aged mice carrying the cyclophilin D-K166R mutation exhibited significantly reduced cognitive impairment.Similarly,administering the mitochondrial permeability transition pore blocker,cyclosporine A,effectively alleviated the decline in synaptic mitochondrial function and cognitive impairment caused by anesthesia and surgery in aged mice.These results indicate that the sirtuin 3/cyclophilin D-K166/mPTP signaling pathway in hippocampal synaptic mitochondria is involved in delayed neurocognitive recovery of aged mice,suggesting this pathway could serve as a potential target for treatment.
基金supported by the Sichuan Provincial Cadre Health Research Project(ZH2024-101)the Key Natural Science Foundation of Sichuan Province(No.2025 ZNSFSC0053)。
文摘BACKGROUND Solid fuel use for cooking and heating is a major environmental risk factor,yet its association with new-onset heart disease(HD)remains unclear.The aim of this study was to investigate the relationship between solid fuel exposure and new-onset HD in a large cohort.METHODS Multivariable logistic regression models assessed associations between cooking/heating fuel types(coal,crop residue/wood,liquefied petroleum gas,natural gas,and others)and new-onset HD.Subgroup analyses explored effect modification by age,sex,education,smoking,alcohol use,and region.RESULTS A prospective cohort study included 5915 participants,with 781 participants(13.2%)developing new-onset HD.Coal use for cooking showed an initial association with new-onset HD risk(OR=1.41,95%CI:1.06–1.86,P=0.02),which attenuated after full adjustment(OR=1.28,95%CI:0.96–1.72,P=0.10).Coal use for heating demonstrated robust associations across all models(OR=1.86,95%CI:1.42–2.43,P<0.001).Crop residue/wood burning for heating was also significant(Model 2:OR=1.40,95%CI:1.06–1.86,P=0.02).Subgroup analyses revealed stronger associations among females,non-smokers,non-drinkers,and less-educated participants.Geographic stratification showed significant associations in southern but not northern regions.CONCLUSIONS Solid fuel use,particularly coal for heating,is associated with increased new-onset HD risk.Reducing solid fuel exposure is crucial for HD prevention in low-resource settings.
基金Natural Science Foundation of Fujian Province(No.2024J011611)Natural Science Foundation of Nanping City(No.N2021J020)。
文摘AIM:To evaluate the visual function and optical quality in adults aged 80y and older following the bilateral implantation of zonal refractive multifocal intraocular lens(IOL,LS-313 MF30).METHODS:A single-center,non-randomized,prospective clinical trial was conducted,involving cataract patients aged 80 y and older.Patients received bilateral implantation of the LS-313 MF30 or CT Asphina 409MP,based on personal preference.Postoperative assessments included uncorrected and corrected visual acuity at distance,intermediate,and near ranges,as well as defocus curve.Subjective evaluations were performed using the visual function(VF-14)questionnaire,spectacle independence rates,and patient satisfaction surveys.Photic phenomena such as glare,halos,and starbursts were also analyzed.RESULTS:The MF30 group(16 eyes from 8 participants,85.38±2.56 y)exhibited superior uncorrected and corrected intermediate and near visual acuity compared to the 409MP group(26 eyes from 13 participants,85.77±2.20 y),while distance visual acuity was comparable between groups.The defocus curve of the MF30 group revealed two peaks at 0.00 D and-3.00 D,indicating a broader depth of focus.Patients in the MF30 group reported higher rates of spectacle independence and greater satisfaction.While photic phenomena such as glare(28.6%vs 18.5%,P=0.584),starburst(9.5%vs 3.7%,P=0.567)and halos(23.8%vs 11.11%,P=0.438)were more prevalent in the MF30 group,they were generally mild and did not significantly impact daily activities.CONCLUSION:Zonal refractive multifocal IOLs provide elderly patients with improved distance and near vision,greater spectacle independence,and greater satisfaction.Although photic phenomena were slightly more frequent with MF30,they are generally reported as non-disruptive and do not affect their daily life compared to monofocal IOLs.
基金supported by a Borden Scholars awardDuke Claude D.Pepper Older Americans Independence Center Pilot Award(P30AG028716)by the NIH/NIA(R01AG081393)。
文摘Advanced age impairs bone fracture healing;the underlying mechanism of this phenomenon remains unknown.We determined that apolipoprotein E(ApoE)increases with age and causes poor fracture healing.After deletion of hepatic ApoE expression(ΔApoE),24-month-oldΔApoE mice displayed a 95%reduction in circulating ApoE levels and significantly improved fracture healing.ApoE treatment of aged BMSCs inhibited osteoblast differentiation in tissue culture models;RNA-seq,Western blot,immunofluorescence,and RT-PCR analyses indicated that the Wnt/β-catenin pathway is the target of this inhibition.Indeed,we showed that ApoE had no effect on cultures with stabilizedβ-catenin levels.Next,we determined that Lrp4 serves as the osteoblast cell surface receptor to ApoE,as expression of Lrp4 is required in ApoE-based inhibition of Wnt/β-catenin signaling and osteoblast differentiation.Importantly,we validated this ApoE-Lrp4-Wnt/β-catenin molecular mechanism in human osteoblast differentiation.Finally,we identified an ApoE-neutralizing antibody(NAb)and used it to treat aged,wildtype mice 3 days after fracture surgery resulting in fracture calluses with 35%more bone deposition.Our work here identifies novel liver-to-bone cross-talk and a noninvasive,translatable therapeutic intervention for aged bone regeneration.
文摘Objective:This study aimed to explore the readiness for advance care planning(ACP)among older adults in Macao’s day service centers and investigate the influencing factors.Methods:A cross-sectional study was conducted from October to December 2022 using a convenience sampling method.A total of 312 older adults were selected from 13 day service centers for older adults in Macao,China.The Advance Care Planning Acceptance Questionnaire and the Family Adaptation,Partnership,Growth,Affection,Resolve(APGAR)Scale were used to survey the older adults.Results:A total of 306 older adults completed the survey.The score for advance care planning readiness was 65.55±10.69,and 59.5%of participants(n=182)were willing to participate in ACP.The family function score was 7.24±2.51,while 70.3%of participants were from a highly functional family.The higher family function indicating a higher readiness for advance care planning(r=0.396,P<0.001).The multiple linear regression analysis indicated that the variables“age,”“knowledge of ACP,”“experience with ACP,”and“received resuscitation of yourself,relatives or friends”combined with“family function”can influence advance care planning readiness among older adults(R^(2)=0.317,F=27.898,P<0.001).Conclusions:Older adults in Macao’s day service centers were willing to engage in ACP.The importance of family involvement is highlighted in the ACP readiness.Health education and improved family communication are vital for promoting ACP,which ensures individuals receive care when they lack the capacity to make that choice.Additionally,healthcare professionals should enhance communication and education with older adults during the medical care process.
基金supported by China Agriculture Research System of MOF and MARA(CARS-40)the National Natural Science Foundation of China(32302776)。
文摘Background Higher embryonic mortality,especially in aged breeding hens,is associated with insufficient hepatic functionality in maintaining redox homeostasis.Our previous study demonstrated that egg exosome-derived miRNAs may play a key role in modulating embryonic oxidation-reduction process,whereas the exact function and mechanism were still poorly understood.The present study aimed to investigate the roles of egg exosome miRNAs in maintaining dynamic equilibrium of free radicals and peroxide agents in embryonic liver,as well as demonstrate the specific mechanism using oxidative stress-challenged hepatocytes.Results Compared to 36-week-old breeding hens,decreased hatchability and increased embryonic mortality were observed in 65-week-old breeding hens.Meanwhile,the older group showed the increased MDA levels and decreased SOD and GSH-Px activities in embryonic liver,muscle and serum.Embryonic mortality was significantly positively correlated with MDA level and negatively correlated with GSH-Px activity in embryonic liver.In addition,363 differentially expressed genes(DEGs)were identified in embryonic liver,13 differentially expressed miRNAs(DE-miRNAs)were identified in egg exosomes.These DEGs and DE-miRNAs were involved in oxidoreductase activity,glutathione metabolic process,MAPK signaling pathway,apoptosis and autophagy.miRNA-mRNA network analysis further found that DEGs targeted by DE-miRNAs were mainly enriched in programmed cell death,such as apoptosis and autophagy.Wherein,MAPK10 with highest MCC and AUC values was significantly related to GSH-Px activity and MDA level,and served as the target gene of miR-145-5p based on dual luciferase reporter experiment and correlation analysis.Bioinformatics analysis found that miR-145-5p/MAPK10 axis might alleviate peroxide generation and apoptosis.In primary hepatocytes of chick embryos,miR-145-5p transfection significantly reversed H_(2)O_(2)-induced mitochondrial ROS increase,MAPK10,BAX and CASP3 overexpression and excessive apoptosis.Conclusion Exosome miR-145-5p in eggs could target MAPK10 and decrease mitochondrial ROS,attenuating oxidative damage and apoptosis in hepatocytes of chick embryos.These findings may provide new theoretical basis for the improvement of maternal physiological status to maintain embryonic redox homeostasis by nutritional or genetic modifications.
文摘Although eye problems can occur at any age, they are often common from the age of 40. Eye diseases with a prevalence associated with age and aging will continue to increase in the coming years. Most studies conducted on problems in middle-aged people have focused on visual disorders without taking into account all the ocular morbidities that may affect this segment of the population, hence the present study, the aim of which is to determine the proportions of different eye diseases in people aged 40 and over. Materials and Methods: This was a descriptive cross-sectional study carried out in the ophthalmology department covering the period from January 1 to December 31, 2020. Results: In total, we collected 828 patients aged 40 and over out of 1811 patients who received ophthalmological consultation during the study period, representing 45.72%. The most represented age group was 40 - 50 years, with an average age of 58.84 years and a maximum of 93 years. There were slightly more women (62.3%) than men (37.7%). The main reasons for consultation were decreased visual acuity (26.4%) and pruritus (19.9%). The main eye diseases diagnosed were cataracts (23%), allergic conjunctivitis (21.1%), and bacterial conjunctivitis (14.2%). Discussions: The predominance of cataracts in the diagnosed diseases confirms the literature data, according to which the main eye morbidities in middle-aged and elderly people are cataracts, glaucoma, and age-related macular degeneration. Conclusion: It is crucial to have a mastery of these epidemiological data of eye diseases in order to adapt the technical platforms of eye care structures to the needs of different segments of the population.
基金funded by the Natural Science Foundation of Hubei Province(2021CFB414)Scientific Research Program of Traditional Chinese Medicine of Hubei Provincial Health and Wellness Commission(ZY2021M074).
文摘Icariin is a natural product that possesses numerous pharmaceutical properties.Thus,this study aimed to investigate the molecular mechanism by which icariin prevents ferroptosis in aged-related osteoporosis.Firstly,mRNA transcriptomics was used to analyze differentially expressed genes and ferroptosis markers.Next,a weighted correlation network analysis was conducted on these genes.Then,common genes among ferroptosis,Yinyanghuo,and differentially expressed genes were identified as target genes.Single-cell and spatial transcriptomics were analyzed to evaluate expression changes of target genes in bone marrow.Furthermore,to validate the results of bioinformatics analysis,MC3T3-E1 cells were used to model the preventive effects of icariin in vitro,and ferroptosis markers and mitochondrial function were both examined.In vivo,4-month-old mice were fed a diet containing icariin for 14 months,following which bone proteomics was assessed to identify essential proteins.Hematoxylin-eosin staining and immunohistochemistry assays were performed on mouse femurs.Finally,Western blot and polymerase chain reaction(PCR)analyses were performed to analyze the effects of icariin on ferroptosis target biomarkers.Ptgs2 and Hmox1 were identified as target genes related to both icariin and ferroptosis.The expression of ferroptosis-related genes was up-regulated with age.Moreover,single-cell and spatial transcriptomics analyses revealed that up-regulation of these two genes inhibited osteogenic capability.The results of the in vitro experiments indicated that icariin mitigated the accumulation of reactive oxygen species and β-galactosidase.Similarly,icariin prevented aberrant changes in the levels of ferroptosis-related proteins,consistent with the results of the in vivo experiments.Specifically,10 mg/(kg·day)of icariin inhibited ferroptosis and osteoporosis in aged mice.Overall,this study revealed that icariin could serve as a dietary supplement to prevent age-induced osteoporosis.Furthermore,Ptgs2 and Hmox1 were identified as ferroptosis-related targets through which icariin exerts its protective effects.
基金the community of older adults who participated in this study.Ministry of Education Science and Technology Industry-University Co-operation Collaborative Education Project(230806630292310).
文摘Background:Falls are a significant public health concern among older adults,often leading to severe injuries and decreased quality of life.Fall prevention self-management behaviors are critical in reducing fall risk.However,the status and influencing factors of these behaviors among community-dwelling older adults in China remain poorly understood.This study aimed to investigate fall prevention self-management behaviors and their influencing factors among community-dwelling older adults in Fuzhou,China.Methods:From June to December 2022,we used multi-stage cluster random sampling and invited 675 community-dwelling older adults from Fujian as participants.We conducted a questionnaire survey using the general information questionnaire,Fall Prevention Self-management Behavior among Older Adults Questionnaire,Questionnaire on Knowledge,Belief,and Practice of Falls Prevention among Older Adults in Community,Modified Falls Efficacy Scale,Social Support Rating Scale,and Chinese Version of the Self-Assessment Fall Risk Scale for Older Adults.Multivariate linear regression was used to identify the factors influencing fall prevention self-management behavior among older adults.Results:The overall score of fall prevention self-management behaviors was 146.81±34.22,indicating a moderate level.Key influencing factors included education level,pre-retirement occupation,income,medication type,self-assessed physical condition,fear of falling,knowledge and practices in fall prevention,social support,and fall risk.These factors collectively explained 34.3%of the variance in self-management behaviors.Conclusion:The findings highlight the need for targeted interventions to enhance fall prevention self-management among community-dwelling older adults.Interventions should focus on improving knowledge,strengthening social support,and addressing psychological barriers such as fear of falling.This study provides valuable insights for developing community-based strategies to reduce fall risks and promote healthy aging.
基金Supported by National Institutes of Health Grants,No.R01DK103055(to Ro S)RosVivo Therapeutics,No.AWD-01-00003158(to Ro S)the National Research Foundation of Korea Grant Funded by the Korean Government(MSIT),No.NRF-2021R1C1C2006743(to Kim MS)and No.NRF-2021R1A2C1095311(to Lee MY).
文摘BACKGROUND We previously identified miR-10b-5p as a key regulator of gastrointestinal(GI)motility through its essential role in the development and function of interstitial cells of Cajal(ICC),the pacemaker cells of the gut.Loss of miR-10b-5p in ICC im-pairs intestinal motility and contributes to constipation,a common condition in the elderly.Notably,miR-10b-5p is co-expressed with its paralog,miR-10a-5p,in ICC.AIM To investigate the roles of miR-10a-5p and miR-10b-5p in age-associated intestinal dysmotility and assess the therapeutic potential of restoring their expression.METHODS We employed aged mice,mir-10a and mir-10b single and double knockout(KO)models,and human plasma and colon samples across age groups.GI and colonic transit,ICC network integrity,and expression levels of miR-10a/b-5p were eva-luated.Additionally,we tested whether treatment with their microRNA mimics could restore GI motility in aged mice.RESULTS Aged mice exhibited delayed GI and colonic transit,reduced fecal output,and diminished expression of miR-10a-5p and miR-10b-5p,which peaked during late embryonic and early postnatal stages and declined with age.This decline para-lleled ICC network deterioration in the colon.All KO models exhibited impaired motility and ICC loss,with mir-10a KO mice displaying more severe phenotypes than mir-10b KO mice.Double KO mice demonstrated growth retardation and reduced survival,with homozygous mutants living only up to 3 months.Treatment of aged mice with miR-10a-5p and miR-10b-5p mimics encapsu-lated in jetPEI significantly improved GI and colonic motility.Successful delivery to the gut,including the colon,was confirmed.In human samples,both miR-10a/b-5p and KIT expression decreased with age.CONCLUSION miR-10a-5p and miR-10b-5p are essential for ICC maintenance and colonic motility,and their age-related decline contributes to GI dysmotility in both mice and humans.Restoring their levels offers a promising therapeutic stra-tegy for treating age-related constipation and other motility disorders.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.52378432 and 52278426)the Key Research and Development Program of Shaanxi Province(Grant No.2022SF-169).
文摘The objective of this paper is to comprehensively review the research progress of bio-oil properties and hot rejuvenation behavior and mechanism to aged asphalt.The preparation process,composition characteristics of bio-oils and their component correspondence with petroleum asphalt were compared.The diffusion and fusion effects of various bio-oils in aged asphalt were introduced.Bio-oil cannot be used as a direct alternative of petroleum asphalt,but it has the potential to effectively rejuvenate aged asphalt binders due to the component similarity with petroleum asphalt and good diffusion properties.For the asphalt rejuvenation,the functionalization treatment methods of bio-oil were discussed such as purification,composition modification and component conversion.The active groups and derivatives in bio-oil can be converted into the missing components of the aged binder through phenolate,grafting,polycondensation,resinifying,but the conversion process and mechanism are still unclear.From the perspectives of diffusion behavior,components regulation,dissolving asphaltene and micro-rejuvenation effect,the rejuvenation behavior and mechanism of bio-oil on aged asphalt were elaborated,and the effects of various types of bio-oil and aged asphalt on rejuvenation behavior were analyzed.The preparation process and dosage of bio-rejuvenator were summarized.The rejuvenation effects of bio-oil on aged asphalt were comprehensively investigated from the aspects of high and low temperature performances,rheological properties,microstructure and chemical composition of bio-rejuvenated asphalt binders.Finally,the limitations of bio-oil used as asphalt rejuvenators were discussed,and future research directions were prospected,which can provide reference and theoretical basis for the development of high-performance bio-oil rejuvenating agents and the engineering application of bio-oil to improve the properties of aged asphalt materials.
基金Supported by the High-level Talent Program of Yangzhou University to Yingying ZHANG。
文摘The widespread production and use of plastic products have highlighted the ecological risks of microplastics(MPs).MPs,mainly as microfibers(MFs),can carry various pollutants,and their properties may change over time,potentially affecting their interactions with pollutants.This study aims to compare the combined toxicity effects of virgin and aged MFs with the common heavy metal copper(Cu).Gibel carp Carassius auratus gibelio were exposed to 1 mg/L of virgin and aged polyacrylonitrile MFs(PanMfs)separately and in combination with Cu(0.5-mg/L copper sulfate)for 21 d.Results show that both aged and virgin PanMfs significantly reduced Cu accumulation in gibel carp,with no notable difference.Cu adversely affected the carp's intestine and liver,while PanMfs alone had minimal histological impact but notably mitigated intestinal damage caused by Cu.Enzyme activity and gene expression analysis indicated that both PanMfs and Cu exposure,alone or combined,compromised gibel carp's antioxidant capacity,digestive and liver functions,induced inflammation,and affected liver metabolism and intestinal immune response.Integrated biomarker response analysis of enzyme activity and gene expression showed that combined Cu and PanMfs exposure had a stronger impact on gibel carp than individual exposure.However,whether exposed individually or in combination,aged PanMfs had a slightly greater impact than virgin PanMfs.This study showed that PanMfs alone exhibit minimal toxic effects on gibel carp but significantly modify Cu toxicity,with negligible differences between aged and virgin PanMfs except for slight disparities in enzyme activity and gene expression.
基金Funded by the National Natural Science Foundation of China(Nos.52378444,52078130)the Natural Science Foundation of Shandong Province(No.ZR2021QE250)。
文摘The use of epoxy resin(EP)to prepare epoxy recycled asphalt mixture can achieve the reuse of 100%reclaimed asphalt pavement(RAP).However,the high stiffness and brittleness of epoxy resin result in insufficient crack resistance of mixture.To address the issue,dry-method styrene-butadiene-styrene(DSBS)and epoxy resin were mixed with aged asphalt to prepare SBS-modified epoxy reclaimed asphalt(SERA).The micro fusion characteristics and mechanical properties of SERA were evaluated,and the optimal DSBS dosage was determined based on various tests.The results show that adding DSBS can enable the tensile toughness and low-temperature performance of SERA with less EP content to reach or exceed the performance level of epoxy reclaimed asphalt(ERA)with higher EP content.At 30%EP content,the recommended dry-method SBS content is 9%;At 40%EP content,the recommended dry-method SBS content is 5%;When the EP content is 50%,the recommended dry-method SBS content is 7%.
文摘Shanxi,where the essence of its mountains and rivers converge,is a treasure trove of cultural and travel marvels.In the timeless embrace of ancient Pingyao,the legacy of Jin merchants echoes through the ages.The Yungang Grottoes,with their solemn Buddhas,stand as timeless sentinels,whispering tales of a millennium's passage.On the sacred slopes of Mount Wutai,the air is scented with incense,and the tranquil spirit of Zen envelops the misty landscape.Journey back to the heart of the Three Jins,where the aroma of aged vinegar lingers in the air,and hidden vistas reveal their profound beauty.Here,you will discover a charm and character like no other,as the spirit of the land unveils its unique poetic elegance and grace.
基金supported by the National Natural Science Foundation of China(grant number:72304153)the Supporting Project of National Natural Science Foundation of China from Nanjing University of Chinese Medicine,China,(grant number:XPT72304153)the University Philosophy and Social Science Research in Jiangsu Province Jiangsu Province,China(grant number:2023SJYB0318)。
文摘Objectives:This prospective cohort study examined the change trajectories of cognitive and physical functions of individuals with motoric cognitive risk(MCR)syndrome,as well as the longitudinal associations between MCR syndrome and changes in cognitive and physical functions,to provide a new perspective on preventing dementia.Methods:Participants were selected from the China Health and Retirement Longitudinal Study(CHARLS).Demographic characteristics,health status,and lifestyle variables were assessed in 2011.MCR syndrome was defined as the presence of subjective cognitive complaints and objective slow gait,with preserved activities of daily living and absence of dementia,and assessed in 2011.Cognitive function,including orientation,attention and calculation,episodic memory,and visuospatial ability,was measured from 2011 to 2018.Physical function,including grip strength,balance ability,and repeated chair stand tests,was measured from 2011 to 2015.Generalized estimating equation was employed to analyze the longitudinal associations between MCR syndrome in 2011 and changes in cognitive functions over 7 years and physical functions over 4 years.Results:Among 4,217 participants,475 had MCR syndrome in 2011.Both participants with MCR syndrome and those without exhibited a decline in both cognitive and physical function over 7 years and 4 years of follow-up,except for fluctuations in visuospatial ability.Non-MCR syndrome participants demonstrated significantly better overall cognitive function in 2018 compared to 2011(Group×Time:B=0.44,P=0.035)than those in the MCR syndrome group.However,participants with non-MCR syndrome demonstrated significantly worse visuospatial ability in 2013(Group×time:B=−0.44,P=0.002)and 2018(Group×time:B=−0.34,P=0.016)compared to those in the MCR syndrome group.Non-MCR syndrome participants demonstrated significantly better performance in repeated chair stand tests in 2013(Group×time:B=0.31,P<0.001)and 2015(Group×time:B=0.37,P<0.001)compared to those in the MCR syndrome group in 2011.Conclusions:Older adults with MCR syndrome experience worse overall cognitive and physical function performance,especially in repeated chair stand tests,than individuals without MCR syndrome over 7-year and 4-year follow-up periods.It is suggested that future interventional studies will target both physical and cognitive functions in MCR syndrome individuals,providing insights for the prevention of dementia.
