With the continuous progress on affinity peptide research,it has become more and more popular in pharmacology and medicine.lt is promising to study these viruses affinity peptide to treat infectious diseases.And the a...With the continuous progress on affinity peptide research,it has become more and more popular in pharmacology and medicine.lt is promising to study these viruses affinity peptide to treat infectious diseases.And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection,treatment as wel as the study on the molecular mechanism of virus affinity peptide.Therefore,we reviewed the bioinformatics pre-diction technologies of computer simulation,molecular docking and homology model-ing,as wel as the research method on analyzing and screening virus affinity pep-tide,such as Phage display technology.展开更多
In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via ...In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via biosensor system . The results obtained in this study indicate that the affinity of immobilised Ni(Ⅱ) ion affinity ligand for these peptides appear to be related to the arrangement of the histidine residues in the peptides. This study first documents the application of biosensor technique for paptide screening.展开更多
Thymidylate synthase(TS),an essential enzyme for catalyzing the biosynthesisof thymidylate,is a critical therapeutic target in cancer therapy.Recent studies have shown that TSfunctions as an RNA-binding protein by int...Thymidylate synthase(TS),an essential enzyme for catalyzing the biosynthesisof thymidylate,is a critical therapeutic target in cancer therapy.Recent studies have shown that TSfunctions as an RNA-binding protein by interacting with two different sequences on its ownmRNA,thus,repressing translational efficiency.In this study,peptides binding TS RNA with highaffinity were isolated using mRNA display from a large peptide library(】10^(13)differentsequences).The randomized library was subjected up to twelve rounds of in vitro selection andamplification.Comparing the amino acid composition of the selected peptides(12th round,R12)withthose from the initial random library(round zero,R0),the basic and aromatic residues in theselected peptides were enriched significantly,suggesting that these peptide regions might beimportant in the peptide-TS mRNA interaction.Categorizing the amino acids at each random positionbased on their physicochemical properties and comparing the distributions with those of the initialrandom pool,an obvious basic charge characteristic was found at positions 1,12,17 and 18,suggestingthat basic side chains participate in RNA binding.Secondary structure prediction showed that theselected peptides of R12 pool represented a helical propensity compared with R0 pool,and the regionswere rich in basic residues.The electrophoretic gel mobility shift and in vitro translation assaysshowed that the peptides selected using mRNA display could bind TS RNA specifically and inhibit thetranslation of TS mRNA.Our results suggested that the identified peptides could be used as new TSinhibitors and developed to a novel class of anticancer agents.展开更多
基金Supported by the Key Science and Technology Program of Henan Province(162102110136)the Science and Technology Fund for Outstanding Young People of Henan Academy of Agricultural Sciences(2016YQ28)
文摘With the continuous progress on affinity peptide research,it has become more and more popular in pharmacology and medicine.lt is promising to study these viruses affinity peptide to treat infectious diseases.And the analysis on the virus affinity peptide with high selectivity and high sensitivity could provide valuable means for disease detection,treatment as wel as the study on the molecular mechanism of virus affinity peptide.Therefore,we reviewed the bioinformatics pre-diction technologies of computer simulation,molecular docking and homology model-ing,as wel as the research method on analyzing and screening virus affinity pep-tide,such as Phage display technology.
文摘In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via biosensor system . The results obtained in this study indicate that the affinity of immobilised Ni(Ⅱ) ion affinity ligand for these peptides appear to be related to the arrangement of the histidine residues in the peptides. This study first documents the application of biosensor technique for paptide screening.
基金Supported by the National Natural Science Foundation of China(Grant No.30472043)the Department of Science and Technology of Shandong Province(Grant No.2004C07)
文摘Thymidylate synthase(TS),an essential enzyme for catalyzing the biosynthesisof thymidylate,is a critical therapeutic target in cancer therapy.Recent studies have shown that TSfunctions as an RNA-binding protein by interacting with two different sequences on its ownmRNA,thus,repressing translational efficiency.In this study,peptides binding TS RNA with highaffinity were isolated using mRNA display from a large peptide library(】10^(13)differentsequences).The randomized library was subjected up to twelve rounds of in vitro selection andamplification.Comparing the amino acid composition of the selected peptides(12th round,R12)withthose from the initial random library(round zero,R0),the basic and aromatic residues in theselected peptides were enriched significantly,suggesting that these peptide regions might beimportant in the peptide-TS mRNA interaction.Categorizing the amino acids at each random positionbased on their physicochemical properties and comparing the distributions with those of the initialrandom pool,an obvious basic charge characteristic was found at positions 1,12,17 and 18,suggestingthat basic side chains participate in RNA binding.Secondary structure prediction showed that theselected peptides of R12 pool represented a helical propensity compared with R0 pool,and the regionswere rich in basic residues.The electrophoretic gel mobility shift and in vitro translation assaysshowed that the peptides selected using mRNA display could bind TS RNA specifically and inhibit thetranslation of TS mRNA.Our results suggested that the identified peptides could be used as new TSinhibitors and developed to a novel class of anticancer agents.
