At Beijing’s large restaurants, the table talk recently has all been about seafood. Some believe that not only are fish from Japan polluted, but that seafood from the Chinese coast is also not safe.Maybe it’s still ...At Beijing’s large restaurants, the table talk recently has all been about seafood. Some believe that not only are fish from Japan polluted, but that seafood from the Chinese coast is also not safe.Maybe it’s still too early to say how serious the Fukushima nuclear accident is,but it can be certain that its influence has moved beyond Japan, helped along withthe Pacific air and ocean current, as traces展开更多
With trade of over $100 billion in 2009, the importance of China and Africa bilateral exchanges are growing daily. This blooming relationship increases the need of understanding the ways of doing business.This regular...With trade of over $100 billion in 2009, the importance of China and Africa bilateral exchanges are growing daily. This blooming relationship increases the need of understanding the ways of doing business.This regular column will provide a practical insight of doing business in China, including tips and advice based on experience.展开更多
Daily separation of rat pups from their mothers while the preweaning period has an impact on behavior and stress response of pups. We hypothesized that maternal separation may create a depression-like state in mother ...Daily separation of rat pups from their mothers while the preweaning period has an impact on behavior and stress response of pups. We hypothesized that maternal separation may create a depression-like state in mother rats from which we separate the rat pups and decrease their reproductive function and fertility. Also, we aimed to evaluate the transmission degree of depression and anxiety across generations. Females aged 3 months were divided in two groups of 10 rats each. After a first mating and a first parturition, we conducted maternal separation 3 h during 22 days for the experienced group while the control group mothers kept their pups. At 4 months of age, females underwent behavioral tests and a second mating. Second generation’s pups were also subjected to behavioral tests. Behavioral tests showed that mothers which experienced separation were more depressive and anxious than control ones, also they had a lower litter size. Rats pups of the second generation whose mothers experienced maternal separation also revealed behavior changes akin to depression and anxiety. Thus, maternal separation causes depressive and anxious-likestates on mother rats which experienced separation and has an impact on their litter size. Also, consequences of maternal separation seem to last throughout generations.展开更多
Dear Editor,Primary bladder neck obstruction(PBNO)affects approximately 28%–54%of men[1].Its etiology remains unclear.Symptoms of PBNO present as voiding(e.g.,decreased force of stream,hesitancy,intermittent stream,a...Dear Editor,Primary bladder neck obstruction(PBNO)affects approximately 28%–54%of men[1].Its etiology remains unclear.Symptoms of PBNO present as voiding(e.g.,decreased force of stream,hesitancy,intermittent stream,and incomplete emptying),storage(e.g.,frequency,urgency,urge incontinence,and nocturia),or a combination of both[2].Untreated PBNO can lead to bladder dysfunction,bladder diverticula,hydronephrosis,and impaired renal function[3].展开更多
RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and ...RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and lethal seedlings. Dek42 was cloned by Mutator tag isolation and further confirmed by an independent mutant allele and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 materials. Dek42 encodes an RRM_RBM48 type RNA-binding protein that localizes to the nucleus. Dek42 is constitutively expressed in various maize tissues. The dek42 mutation caused a significant reduction in the accumulation of DEK42 protein in mutant kernels. RNA-seq analysis showed that the dek42 mutation significantly disturbed the expression of thousands of genes during maize kernel development. Sequence analysis also showed that the dek42 mutation significantly changed alternative splicing in expressed genes, which were especially enriched for the U12-type intron-retained type. Yeast two-hybrid screening identified SF3a1 as a DEK42-interacting protein. DEK42 also interacts with the spliceosome component U1-70K. These results suggested that DEK42 participates in the regulation of pre-messenger RNA splicing through its interaction with other spliceosome components. This study showed the function of a newly identified RBP and provided insights into alternative splicing regulation during maize kernel development.展开更多
Plant species exhibit substantial variation in leaf morphology.VWe isolated a recessive mutant gene termed small and cordate leaf 1（sclh）that causes alteration in both leaf size and shape of cucumber.Compared to wil...Plant species exhibit substantial variation in leaf morphology.VWe isolated a recessive mutant gene termed small and cordate leaf 1（sclh）that causes alteration in both leaf size and shape of cucumber.Compared to wild type leaves,the sclh mutant had fewer numbers of epidermal pavement cells.A single nucleotide polymorphism was associated with this leaf phenotype,which occurred in a putative nucleoside bisphosphate phosphatase.RNA-seq analysis of the wild type and sclh mutant leaves suggested that SCL;regulation may not involve known hormonal pathways.Our work identified a candidate gene for SCL;that may play a role in leaf development.展开更多
Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in ...Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpα1, which encodes a plastidic isoform of PK （PKp）. OsPKpα1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpα1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein- protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.展开更多
The actinomycete Streptomyces lydicus A_(01) promotes tomato seedling growth; however, the underlying mechanism is unclear. In this study, we investigated whether changes in soil microbial diversity, following Strepto...The actinomycete Streptomyces lydicus A_(01) promotes tomato seedling growth; however, the underlying mechanism is unclear. In this study, we investigated whether changes in soil microbial diversity, following Streptomyces lydicus A_(01) treatment, were responsible for the increased tomato seedling growth. Eukaryotic 18 S ribosomal DNA(rDNA) sequencing showed that S. lydicus A_(01)-treated and untreated soil shared 193 operational taxonomic units(OTUs), whereas bacterial 16 S rDNA sequencing identified 1,219 shared OTUs between the treated and untreated soil. Of the 42 dominant eukaryotic OTUs, eight were significantly increased and six were significantly decreased after A_(01) treatment. Of the 25 dominant bacterial OTUs, 12 were significantly increased and eight were significantly decreased after A_(01) treatment.Most of the eukaryotes and bacteria that increased in abundance exhibited growth promoting characteristics,which were mainly predicted to be associated with mineralization of nitrogen and phosphorus, phosphate solubilization, nutrient accumulation, and secretion of auxin, whereas some were related to plant protection,such as the degradation of toxic and hazardous substances. Soil composition tests showed that S. lydicus A_(01) treatment enhanced the utilization of nitrogen, phosphorus, and potassium in tomato seedlings. Thus, microbial fertilizers based on S. lydicus A_(01) may improve plant growth, without the detriment effects of chemical fertilizers.展开更多
Summary Epigenetic gene variants, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Here, we identify a natural epiallele of OsAK1, which encodes a rice adenylate kinase. The Epiak1 plants...Summary Epigenetic gene variants, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Here, we identify a natural epiallele of OsAK1, which encodes a rice adenylate kinase. The Epiak1 plants show albino in young leaf and panicle with abnormal chloroplast structures.展开更多
Ocean energy has progressively gained considerable interest due to its sufficient potential to meet the world’s energy demand,and the blade is the core component in electricity generation from the ocean current.Howev...Ocean energy has progressively gained considerable interest due to its sufficient potential to meet the world’s energy demand,and the blade is the core component in electricity generation from the ocean current.However,the widened hydraulic excitation frequency may satisfy the blade resonance due to the time variation in the velocity and angle of attack of the ocean current,even resulting in blade fatigue and destructively interfering with grid stability.A key parameter that determines the resonance amplitude of the blade is the hydrodynamic damping ratio(HDR).However,HDR is difficult to obtain due to the complex fluid-structure interaction(FSI).Therefore,a literature review was conducted on the hydrodynamic damping characteristics of blade-like structures.The experimental and simulation methods used to identify and obtain the HDR quantitatively were described,placing emphasis on the experimental processes and simulation setups.Moreover,the accuracy and efficiency of different simulation methods were compared,and the modal work approach was recommended.The effects of key typical parameters,including flow velocity,angle of attack,gap,rotational speed,and cavitation,on the HDR were then summarized,and the suggestions on operating conditions were presented from the perspective of increasing the HDR.Subsequently,considering multiple flow parameters,several theoretical derivations and semi-empirical prediction formulas for HDR were introduced,and the accuracy and application were discussed.Based on the shortcomings of the existing research,the direction of future research was finally determined.The current work offers a clear understanding of the HDR of blade-like structures,which could improve the evaluation accuracy of flow-induced vibration in the design stage.展开更多
1.A.Mertha,“‘Stressing Out’:Cadre Calibration and Affective Proximity to the CCP in Reform-Era China”,The China Quarterly,Vol.229,2017,pp.64-85.2.B.L.McCormick,“Book Review of‘The Chinese Communist Party's C...1.A.Mertha,“‘Stressing Out’:Cadre Calibration and Affective Proximity to the CCP in Reform-Era China”,The China Quarterly,Vol.229,2017,pp.64-85.2.B.L.McCormick,“Book Review of‘The Chinese Communist Party's Capacity to Rule:Ideology,Legitimacy and Party Cohesion’”,The China Journal,Vol.77,2017,pp.161-163.展开更多
This paper presents a study of the effect of cultur-al background on TOEFL scores.It proceeds from therelation between culture and language,then illus-trates with actual questions from various sections ofTOEFL tests h...This paper presents a study of the effect of cultur-al background on TOEFL scores.It proceeds from therelation between culture and language,then illus-trates with actual questions from various sections ofTOEFL tests how American cultural background exertsa remarkable influence on TOEFL scores,and con-cludes with revelations with regard to English teachingin this country.展开更多
The Brief Interactive Optimism Scale (BIOS-G), recently published, was developed to measure optimism based on the interactive personality styles theory in the general population of Mexican adults. It has been validate...The Brief Interactive Optimism Scale (BIOS-G), recently published, was developed to measure optimism based on the interactive personality styles theory in the general population of Mexican adults. It has been validated in both the general population in Mexico and other Latin American countries. This study aims to further consolidate the validity of the BIOS-G in a specific population. The objectives are to assess the psychometric properties of its items, validate its unidimensional structure, describe its distribution, verify its internal consistency, and examine its direct relationship with affective balance and positive affect, as well as its inverse relationship with emotional exhaustion and negative affect among Mexican university teachers. The BIOS-G, the CESQT emotional exhaustion subscale, and Scale of Positive and Negative Experience (SPANE) were administered to a non-probability sample of 213 Mexican university teachers. All items demonstrated discriminative power and internal consistency. The scale showed internal consistency (Green-Yang ordinal ω = 0.74 and McDonald ordinal ω = 0.88). The one-factor model presented good to acceptable fit to the data, with an average variance extracted of 0.65. Its distribution was negatively skewed and mesokurtic. The BIOS-G correlated directly with SPANE’s affective balance and positive emotional experiences and inversely with negative emotional experiences and emotional exhaustion, with moderate strengths of association. Its mean score was higher in men than in women and correlated positively with age. It is concluded that the BIOS-G demonstrates internal consistency and validity evidence among Mexican university teachers, and its use in this specific population is recommended.展开更多
The dynamics of student engagement and emotional states significantly influence learning outcomes.Positive emotions resulting from successful task completion stand in contrast to negative affective states that arise f...The dynamics of student engagement and emotional states significantly influence learning outcomes.Positive emotions resulting from successful task completion stand in contrast to negative affective states that arise from learning struggles or failures.Effective transitions to engagement occur upon problem resolution,while unresolved issues lead to frustration and subsequent boredom.This study proposes a Convolutional Neural Networks(CNN)based approach utilizing the Multi⁃source Academic Affective Engagement Dataset(MAAED)to categorize facial expressions into boredom,confusion,frustration,and yawning.This method provides an efficient and objective way to assess student engagement by extracting features from facial images.Recognizing and addressing negative affective states,such as confusion and boredom,is fundamental in creating supportive learning environments.Through automated frame extraction and model comparison,this study demonstrates reduced loss values with improving accuracy,showcasing the effectiveness of this method in objectively evaluating student engagement.Monitoring facial engagement with CNN using the MAAED dataset is essential for gaining insights into human behaviour and improving educational experiences.展开更多
For the following decades after the American Civil War,most American literature works presented the war in a romantic or sentimental manner.However,Stephen Crane’s The Red Badge of Courage gave it a naturalistic touc...For the following decades after the American Civil War,most American literature works presented the war in a romantic or sentimental manner.However,Stephen Crane’s The Red Badge of Courage gave it a naturalistic touch to tell the story of Henry Fleming,the main character,overcoming cowardice and becoming a hero.Through its revolutionary criticism of psychoanalysis and capitalism,Deleuze and Guattari contributed a legacy to postmodern philosophy with their affect philosophy,with virtuality at its pivot.Using the Deleuzo-Guattarian affective philosophy as a scope,it is clear that the maturing of Henry is in fact a sequence of two attempts of deterritorialization in the war,one failed and the other successful.The heroism Henry acquires is demonstrated in the form of virtual death and disconnection with meaning;nonetheless,his return to the society makes it an incomplete destratification,which,at the same time,indicates the opportunity to be a true hero is forever lost to Henry,lost on the battlefield.展开更多
Under the background of Artificial Intelligence(AI)and medical data informationization,intelligent Traditional Chinese Medicine(TCM)emerges as a new medical model.In this study,we introduce Affective Computing into th...Under the background of Artificial Intelligence(AI)and medical data informationization,intelligent Traditional Chinese Medicine(TCM)emerges as a new medical model.In this study,we introduce Affective Computing into the diagnosis and treatment process of TCM FiveTone Therapy.And proposes an intelligent diagnosis and treatment system including Five-tone diagnosis,monitoring and treatment,to achieve intelligent music matching.This system based on sound-image database for standardized collection and diagnosis of the Five-tone.The assessment and monitoring process are then visually presented,and an emotion analysis model is established by using affective computing.On the other hand,the system employs virtual reality(VR)visual media music and somatosensory music to play and intelligently match the Five-tone,solving issues in the clinical treatment of Five-tone therapy,such as the difficulties of quantifying emotional data,limited ability for generalization feature extraction,and relatively insufficient research on the intelligence of Five-tone therapy.This study can promote the integration of technology in the fields of healthcare,information,and music,thereby enhancing the accuracy of diagnosis and treatment.It holds significant importance in advancing the intelligent development of Traditional Chinese Medicine diagnosis and treatment.展开更多
Considering the comprehensive morphology and genesis of Podzols of the Stolowe Mountains,and the still-possible impact of frost actions and other processes related to cold climate on these soils,the main aims of this ...Considering the comprehensive morphology and genesis of Podzols of the Stolowe Mountains,and the still-possible impact of frost actions and other processes related to cold climate on these soils,the main aims of this study were to determine whether(i)the heterogeneous Podzols in the Stolowe Mountains underwent a phase of development in a cold climate,resulting in frost action features visible on the micromorphological level,and whether(ii)contemporary cryopedogenic traces are masked by the translocation of organic matter due to the podzolisation process.Four soil profiles were investigated,revealing distinct layers corresponding to different periods of soil formation.Under field observation,no explicit frost-related characteristics were observed.Nevertheless,micromorphological analysis revealed cappings of fine materials on grains or peds,as well as development of granostriated(or any striated)b-fabric that resulted from the alternating effects of thawing and freezing processes.Moreover,micromorphological analysis revealed the presence of microstructures that could be the result of cryogenic processes,such as platy,angular blocky and lenticular features,as well as plane,vugh and star-shaped void types.The translocation of organic matter during podzolisation modified or concealed the frost-related features that developed during the late Pleistocene and early Holocene.This is evident,for instance,in the accumulation of organic matter on cappings and within soil voids,which further hinders the identification of frost-related characteristics and the interpretation of the soil's evolution.Macromorphological observations enhanced with micromorphological analyses revealed three distinct layers:(i)a young upper layer composed of loose,sandy material;(ii)a deeper layer containing a spodic horizon with frost actions,involving pedofeatures associated with the Pleistocene cold climate and(iii)a deeper subsoil basal layer.The abovementioned microstructures,combined with lithological discontinuity,support the hypothesised polygenetic origin of Podzols in the studied region.展开更多
This paper is dedicated to constructing a theoretical framework for the identification and treatment of affective disorders in traditional Chinese medicine based on the“five-organ view”.Through in-depth analysis of ...This paper is dedicated to constructing a theoretical framework for the identification and treatment of affective disorders in traditional Chinese medicine based on the“five-organ view”.Through in-depth analysis of the theoretical connotation of the“five-organ concept”,we discussed the characteristics of the five-organ mechanism of affective-philosophical disorders in detail,systematically constructed a system of identification based on the association of the five organs,and proposed a comprehensive and holistic treatment strategy.The results of the study clearly show that the theoretical framework can provide systematic theoretical guidance for the clinical diagnosis and treatment of affective-philosophical disorders in Chinese medicine,help to improve the diagnostic and therapeutic effects of affective-philosophical disorders,and provide new ideas and methods for the theoretical development and clinical practice of affective-philosophical disorders in Chinese medicine,which is of important theoretical and practical significance,and can further promote the modernization of the development of affective-philosophical disorders in Chinese medicine.展开更多
Objectives:Recent research has shown that college students experience high levels of stress from various sources,which contribute to heightened rates of psychological distress and make them particularly vulnerable to ...Objectives:Recent research has shown that college students experience high levels of stress from various sources,which contribute to heightened rates of psychological distress and make them particularly vulnerable to mental health issues.Hence,we conducted a study,aimed to analyze the influence of a mobile-based walking routine on mental health outcomes,such as perceived stress and positive and negative affect.Methods:The participants were divided into two groups:the walking group(N=23)and the control group(N=24).The walking group engaged in an approximately 25-min walking session for at least 3 days per week,which was carefully monitored and facilitated with the help of the Nike Run Club application.The statistical analysis of the quantitative data was carried out with the help of SPSS 25.0.In addition to calculating descriptive statistics,we conducted an independent samples t-test,a repeated-measures ANOVA,and a paired sample t-test to analyze the effectiveness of the intervention.Results:A repeated-measures ANOVA revealed that the walking group exhibited significant reductions in perceived stress(t=2.03,p=0.049,Cohen’s D=0.60)and negative affect(t=2.19,p=0.033,Cohen’s D=0.65)after the intervention,whereas the control group showed no significant changes across time in any of the proposed variables.Conclusion:The results of the study continue to be indicative of the positive effects of regular walking on perceived stress and negative affect,preserving its role as a straightforward and accessible tool for reducing stress and negative affect.展开更多
文摘At Beijing’s large restaurants, the table talk recently has all been about seafood. Some believe that not only are fish from Japan polluted, but that seafood from the Chinese coast is also not safe.Maybe it’s still too early to say how serious the Fukushima nuclear accident is,but it can be certain that its influence has moved beyond Japan, helped along withthe Pacific air and ocean current, as traces
文摘With trade of over $100 billion in 2009, the importance of China and Africa bilateral exchanges are growing daily. This blooming relationship increases the need of understanding the ways of doing business.This regular column will provide a practical insight of doing business in China, including tips and advice based on experience.
文摘Daily separation of rat pups from their mothers while the preweaning period has an impact on behavior and stress response of pups. We hypothesized that maternal separation may create a depression-like state in mother rats from which we separate the rat pups and decrease their reproductive function and fertility. Also, we aimed to evaluate the transmission degree of depression and anxiety across generations. Females aged 3 months were divided in two groups of 10 rats each. After a first mating and a first parturition, we conducted maternal separation 3 h during 22 days for the experienced group while the control group mothers kept their pups. At 4 months of age, females underwent behavioral tests and a second mating. Second generation’s pups were also subjected to behavioral tests. Behavioral tests showed that mothers which experienced separation were more depressive and anxious than control ones, also they had a lower litter size. Rats pups of the second generation whose mothers experienced maternal separation also revealed behavior changes akin to depression and anxiety. Thus, maternal separation causes depressive and anxious-likestates on mother rats which experienced separation and has an impact on their litter size. Also, consequences of maternal separation seem to last throughout generations.
文摘Dear Editor,Primary bladder neck obstruction(PBNO)affects approximately 28%–54%of men[1].Its etiology remains unclear.Symptoms of PBNO present as voiding(e.g.,decreased force of stream,hesitancy,intermittent stream,and incomplete emptying),storage(e.g.,frequency,urgency,urge incontinence,and nocturia),or a combination of both[2].Untreated PBNO can lead to bladder dysfunction,bladder diverticula,hydronephrosis,and impaired renal function[3].
基金supported by the National Key Research and Development Program of China (2016YFD0101003)the National Natural Science Foundation of China (91635303 and 31425019)
文摘RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and lethal seedlings. Dek42 was cloned by Mutator tag isolation and further confirmed by an independent mutant allele and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 materials. Dek42 encodes an RRM_RBM48 type RNA-binding protein that localizes to the nucleus. Dek42 is constitutively expressed in various maize tissues. The dek42 mutation caused a significant reduction in the accumulation of DEK42 protein in mutant kernels. RNA-seq analysis showed that the dek42 mutation significantly disturbed the expression of thousands of genes during maize kernel development. Sequence analysis also showed that the dek42 mutation significantly changed alternative splicing in expressed genes, which were especially enriched for the U12-type intron-retained type. Yeast two-hybrid screening identified SF3a1 as a DEK42-interacting protein. DEK42 also interacts with the spliceosome component U1-70K. These results suggested that DEK42 participates in the regulation of pre-messenger RNA splicing through its interaction with other spliceosome components. This study showed the function of a newly identified RBP and provided insights into alternative splicing regulation during maize kernel development.
基金supported by grants from National Natural Science Foundation of China(31601773)
文摘Plant species exhibit substantial variation in leaf morphology.VWe isolated a recessive mutant gene termed small and cordate leaf 1（sclh）that causes alteration in both leaf size and shape of cucumber.Compared to wild type leaves,the sclh mutant had fewer numbers of epidermal pavement cells.A single nucleotide polymorphism was associated with this leaf phenotype,which occurred in a putative nucleoside bisphosphate phosphatase.RNA-seq analysis of the wild type and sclh mutant leaves suggested that SCL;regulation may not involve known hormonal pathways.Our work identified a candidate gene for SCL;that may play a role in leaf development.
基金supported by grants from the National Key Research and Development Program of China(2016YFD0100101-08)the National Transformation Science and Technology Program(2016ZX08001006)+3 种基金the Jiangsu Science and Technology Development Program(BE2015363)the Agricultural Science and Technology Innovation Fund project of Jiangsu Province(CX(16)1029)the Key Laboratory of Biology,Genetics and Breeding of Japonica Rice in the Mid-lower Yangtze River,Ministry of Agriculture,Chinathe Jiangsu Collaborative Innovation Center for Modern Crop Production
文摘Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpα1, which encodes a plastidic isoform of PK （PKp）. OsPKpα1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpα1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein- protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.
基金supported by the National Key Research and Development Program of China (Synergism of Live Bio-pesticides and Related Mechanism of Ecological Regulation on Pest, No.2017YFD0200403 The new highly efficient bio-fungicide, No.2017YFD0201108)+4 种基金Beijing Municipal Science and Technology Plan Projects (No.D151100003915002, D151100003915003)National Natural Science Foundation of China (No.31270155)Beijing Natural Science Foundation (No.6152007)Special Project for Innovation Ability Construction of BAAFS (No.KJCX20170415)the National Special Project of Basic Work Project for Science and Technology (No.2014FY120900)
文摘The actinomycete Streptomyces lydicus A_(01) promotes tomato seedling growth; however, the underlying mechanism is unclear. In this study, we investigated whether changes in soil microbial diversity, following Streptomyces lydicus A_(01) treatment, were responsible for the increased tomato seedling growth. Eukaryotic 18 S ribosomal DNA(rDNA) sequencing showed that S. lydicus A_(01)-treated and untreated soil shared 193 operational taxonomic units(OTUs), whereas bacterial 16 S rDNA sequencing identified 1,219 shared OTUs between the treated and untreated soil. Of the 42 dominant eukaryotic OTUs, eight were significantly increased and six were significantly decreased after A_(01) treatment. Of the 25 dominant bacterial OTUs, 12 were significantly increased and eight were significantly decreased after A_(01) treatment.Most of the eukaryotes and bacteria that increased in abundance exhibited growth promoting characteristics,which were mainly predicted to be associated with mineralization of nitrogen and phosphorus, phosphate solubilization, nutrient accumulation, and secretion of auxin, whereas some were related to plant protection,such as the degradation of toxic and hazardous substances. Soil composition tests showed that S. lydicus A_(01) treatment enhanced the utilization of nitrogen, phosphorus, and potassium in tomato seedlings. Thus, microbial fertilizers based on S. lydicus A_(01) may improve plant growth, without the detriment effects of chemical fertilizers.
基金supported by Genetically Modified Breeding Major Projects (grant no.2016ZX08009001005 to X.Song)The National Key Research Program of China (2016YFD0100904 to X.Cao)+1 种基金the National Natural Science Foundation of China (No.31161140348 to P.Hu, 31521064 to S.Tang and 31300987 to X.Song)the State Key Laboratory of Plant Genomics
文摘Summary Epigenetic gene variants, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Here, we identify a natural epiallele of OsAK1, which encodes a rice adenylate kinase. The Epiak1 plants show albino in young leaf and panicle with abnormal chloroplast structures.
基金Supported by the National Natural Science Foundation of China(Nos.52222904 and 52309117)China Postdoctoral Science Foundation(Nos.2022TQ0168 and 2023M731895).
文摘Ocean energy has progressively gained considerable interest due to its sufficient potential to meet the world’s energy demand,and the blade is the core component in electricity generation from the ocean current.However,the widened hydraulic excitation frequency may satisfy the blade resonance due to the time variation in the velocity and angle of attack of the ocean current,even resulting in blade fatigue and destructively interfering with grid stability.A key parameter that determines the resonance amplitude of the blade is the hydrodynamic damping ratio(HDR).However,HDR is difficult to obtain due to the complex fluid-structure interaction(FSI).Therefore,a literature review was conducted on the hydrodynamic damping characteristics of blade-like structures.The experimental and simulation methods used to identify and obtain the HDR quantitatively were described,placing emphasis on the experimental processes and simulation setups.Moreover,the accuracy and efficiency of different simulation methods were compared,and the modal work approach was recommended.The effects of key typical parameters,including flow velocity,angle of attack,gap,rotational speed,and cavitation,on the HDR were then summarized,and the suggestions on operating conditions were presented from the perspective of increasing the HDR.Subsequently,considering multiple flow parameters,several theoretical derivations and semi-empirical prediction formulas for HDR were introduced,and the accuracy and application were discussed.Based on the shortcomings of the existing research,the direction of future research was finally determined.The current work offers a clear understanding of the HDR of blade-like structures,which could improve the evaluation accuracy of flow-induced vibration in the design stage.
文摘1.A.Mertha,“‘Stressing Out’:Cadre Calibration and Affective Proximity to the CCP in Reform-Era China”,The China Quarterly,Vol.229,2017,pp.64-85.2.B.L.McCormick,“Book Review of‘The Chinese Communist Party's Capacity to Rule:Ideology,Legitimacy and Party Cohesion’”,The China Journal,Vol.77,2017,pp.161-163.
文摘This paper presents a study of the effect of cultur-al background on TOEFL scores.It proceeds from therelation between culture and language,then illus-trates with actual questions from various sections ofTOEFL tests how American cultural background exertsa remarkable influence on TOEFL scores,and con-cludes with revelations with regard to English teachingin this country.
文摘The Brief Interactive Optimism Scale (BIOS-G), recently published, was developed to measure optimism based on the interactive personality styles theory in the general population of Mexican adults. It has been validated in both the general population in Mexico and other Latin American countries. This study aims to further consolidate the validity of the BIOS-G in a specific population. The objectives are to assess the psychometric properties of its items, validate its unidimensional structure, describe its distribution, verify its internal consistency, and examine its direct relationship with affective balance and positive affect, as well as its inverse relationship with emotional exhaustion and negative affect among Mexican university teachers. The BIOS-G, the CESQT emotional exhaustion subscale, and Scale of Positive and Negative Experience (SPANE) were administered to a non-probability sample of 213 Mexican university teachers. All items demonstrated discriminative power and internal consistency. The scale showed internal consistency (Green-Yang ordinal ω = 0.74 and McDonald ordinal ω = 0.88). The one-factor model presented good to acceptable fit to the data, with an average variance extracted of 0.65. Its distribution was negatively skewed and mesokurtic. The BIOS-G correlated directly with SPANE’s affective balance and positive emotional experiences and inversely with negative emotional experiences and emotional exhaustion, with moderate strengths of association. Its mean score was higher in men than in women and correlated positively with age. It is concluded that the BIOS-G demonstrates internal consistency and validity evidence among Mexican university teachers, and its use in this specific population is recommended.
文摘The dynamics of student engagement and emotional states significantly influence learning outcomes.Positive emotions resulting from successful task completion stand in contrast to negative affective states that arise from learning struggles or failures.Effective transitions to engagement occur upon problem resolution,while unresolved issues lead to frustration and subsequent boredom.This study proposes a Convolutional Neural Networks(CNN)based approach utilizing the Multi⁃source Academic Affective Engagement Dataset(MAAED)to categorize facial expressions into boredom,confusion,frustration,and yawning.This method provides an efficient and objective way to assess student engagement by extracting features from facial images.Recognizing and addressing negative affective states,such as confusion and boredom,is fundamental in creating supportive learning environments.Through automated frame extraction and model comparison,this study demonstrates reduced loss values with improving accuracy,showcasing the effectiveness of this method in objectively evaluating student engagement.Monitoring facial engagement with CNN using the MAAED dataset is essential for gaining insights into human behaviour and improving educational experiences.
文摘For the following decades after the American Civil War,most American literature works presented the war in a romantic or sentimental manner.However,Stephen Crane’s The Red Badge of Courage gave it a naturalistic touch to tell the story of Henry Fleming,the main character,overcoming cowardice and becoming a hero.Through its revolutionary criticism of psychoanalysis and capitalism,Deleuze and Guattari contributed a legacy to postmodern philosophy with their affect philosophy,with virtuality at its pivot.Using the Deleuzo-Guattarian affective philosophy as a scope,it is clear that the maturing of Henry is in fact a sequence of two attempts of deterritorialization in the war,one failed and the other successful.The heroism Henry acquires is demonstrated in the form of virtual death and disconnection with meaning;nonetheless,his return to the society makes it an incomplete destratification,which,at the same time,indicates the opportunity to be a true hero is forever lost to Henry,lost on the battlefield.
基金Project of Jiangsu Province Technology Engineering Research Center of Traditional Chinese Medicine Health PreservationScientists Fund of the Natural Science Foundation of Nanjing University of Chinese Medicine:to Study the Mechanism of Five Elements Music in the Treatment of Post-stroke Depression based on Metabolomics-based Study(NZY81804161)Major Project of Philosophy and Social Sciences Fund for Universities in Jiangsu Province:Research on Ancient Chinese Music Therapy Thought(2024SJZD150)。
文摘Under the background of Artificial Intelligence(AI)and medical data informationization,intelligent Traditional Chinese Medicine(TCM)emerges as a new medical model.In this study,we introduce Affective Computing into the diagnosis and treatment process of TCM FiveTone Therapy.And proposes an intelligent diagnosis and treatment system including Five-tone diagnosis,monitoring and treatment,to achieve intelligent music matching.This system based on sound-image database for standardized collection and diagnosis of the Five-tone.The assessment and monitoring process are then visually presented,and an emotion analysis model is established by using affective computing.On the other hand,the system employs virtual reality(VR)visual media music and somatosensory music to play and intelligently match the Five-tone,solving issues in the clinical treatment of Five-tone therapy,such as the difficulties of quantifying emotional data,limited ability for generalization feature extraction,and relatively insufficient research on the intelligence of Five-tone therapy.This study can promote the integration of technology in the fields of healthcare,information,and music,thereby enhancing the accuracy of diagnosis and treatment.It holds significant importance in advancing the intelligent development of Traditional Chinese Medicine diagnosis and treatment.
基金financed by the Wroclaw University of Environmental and Life Sciences(Poland)。
文摘Considering the comprehensive morphology and genesis of Podzols of the Stolowe Mountains,and the still-possible impact of frost actions and other processes related to cold climate on these soils,the main aims of this study were to determine whether(i)the heterogeneous Podzols in the Stolowe Mountains underwent a phase of development in a cold climate,resulting in frost action features visible on the micromorphological level,and whether(ii)contemporary cryopedogenic traces are masked by the translocation of organic matter due to the podzolisation process.Four soil profiles were investigated,revealing distinct layers corresponding to different periods of soil formation.Under field observation,no explicit frost-related characteristics were observed.Nevertheless,micromorphological analysis revealed cappings of fine materials on grains or peds,as well as development of granostriated(or any striated)b-fabric that resulted from the alternating effects of thawing and freezing processes.Moreover,micromorphological analysis revealed the presence of microstructures that could be the result of cryogenic processes,such as platy,angular blocky and lenticular features,as well as plane,vugh and star-shaped void types.The translocation of organic matter during podzolisation modified or concealed the frost-related features that developed during the late Pleistocene and early Holocene.This is evident,for instance,in the accumulation of organic matter on cappings and within soil voids,which further hinders the identification of frost-related characteristics and the interpretation of the soil's evolution.Macromorphological observations enhanced with micromorphological analyses revealed three distinct layers:(i)a young upper layer composed of loose,sandy material;(ii)a deeper layer containing a spodic horizon with frost actions,involving pedofeatures associated with the Pleistocene cold climate and(iii)a deeper subsoil basal layer.The abovementioned microstructures,combined with lithological discontinuity,support the hypothesised polygenetic origin of Podzols in the studied region.
文摘This paper is dedicated to constructing a theoretical framework for the identification and treatment of affective disorders in traditional Chinese medicine based on the“five-organ view”.Through in-depth analysis of the theoretical connotation of the“five-organ concept”,we discussed the characteristics of the five-organ mechanism of affective-philosophical disorders in detail,systematically constructed a system of identification based on the association of the five organs,and proposed a comprehensive and holistic treatment strategy.The results of the study clearly show that the theoretical framework can provide systematic theoretical guidance for the clinical diagnosis and treatment of affective-philosophical disorders in Chinese medicine,help to improve the diagnostic and therapeutic effects of affective-philosophical disorders,and provide new ideas and methods for the theoretical development and clinical practice of affective-philosophical disorders in Chinese medicine,which is of important theoretical and practical significance,and can further promote the modernization of the development of affective-philosophical disorders in Chinese medicine.
基金supported by the Bio&Medical Technology Development Program of the National Research Foundation(NRF)&funded by the Korean government(MSIT)(NRF-2021M3A9E4080780)Hankuk University of Foreign Studies(2024).
文摘Objectives:Recent research has shown that college students experience high levels of stress from various sources,which contribute to heightened rates of psychological distress and make them particularly vulnerable to mental health issues.Hence,we conducted a study,aimed to analyze the influence of a mobile-based walking routine on mental health outcomes,such as perceived stress and positive and negative affect.Methods:The participants were divided into two groups:the walking group(N=23)and the control group(N=24).The walking group engaged in an approximately 25-min walking session for at least 3 days per week,which was carefully monitored and facilitated with the help of the Nike Run Club application.The statistical analysis of the quantitative data was carried out with the help of SPSS 25.0.In addition to calculating descriptive statistics,we conducted an independent samples t-test,a repeated-measures ANOVA,and a paired sample t-test to analyze the effectiveness of the intervention.Results:A repeated-measures ANOVA revealed that the walking group exhibited significant reductions in perceived stress(t=2.03,p=0.049,Cohen’s D=0.60)and negative affect(t=2.19,p=0.033,Cohen’s D=0.65)after the intervention,whereas the control group showed no significant changes across time in any of the proposed variables.Conclusion:The results of the study continue to be indicative of the positive effects of regular walking on perceived stress and negative affect,preserving its role as a straightforward and accessible tool for reducing stress and negative affect.
