Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to all...Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to alleviate obesity by modulating the microbiota.In this study,we found that oral administration of Bifidobacterium adolescentis CCFM8630 to obese mice inhibited high-fat diet(HFD)-induced changes in body weight and adipose tissue and alleviated hepatic oxidative stress.Furthermore,B.adolescentis CCFM8630 treatment primarily affected the relative abundances of the phyla Proteobacteria and Actinobacteria,and thereby decreased the production of lipopolysaccharide(LPS)and the occurrence of LPS related diseases.A high fiber intake increased the abundance of Lactobacillus and the concentrations of short chain fatty acids in obese mice,but these changes were reversed by B.adolescentis CCFM8630 treatment.In addition,targeted metabolomic analysis and microbiota relationship analysis revealed that B.adolescentis CCFM8630 treatment modified the microbiota of obese mice by promoting the conversion of tryptophan(Trp)to xanthurenic acid,kynurenic acid,tryptamine,indole-3-acetic acid,and indole-3-carboxaldehyde;facilitated the expression of interleukin-17A and the aryl hydrocarbon receptor to generate interleukin-22 in the colon;and upregulated the expression of tight junction proteins,thereby strengthening intestinal barriers.In summary,our findings suggest that the intake of B.adolescentis CCFM8630 may alleviate obesity by modulating the gut microbiota and related Trp metabolism.展开更多
In order to study the structure of lotus （Nelumbo nucifera Gaertn） seed oligosaccharides and their effect on the proliferation ofBifidobacterium adolescentis, we extracted the oligosaccharides from seeds collected f...In order to study the structure of lotus （Nelumbo nucifera Gaertn） seed oligosaccharides and their effect on the proliferation ofBifidobacterium adolescentis, we extracted the oligosaccharides from seeds collected from Jianning County, China. We preliminarily characterized the groups, molecular weights, molecular formulae, component monosaccharides and glycosidic bonds using mass spectrometry （MS） and nuclear magnetic resonance （NMR） after isolation and purification. The lotus seed oligosaccharides contained glycosidic bonds Manp-（1→）, Galp-（1→）, α（1→6）-Glup and α（1→6）-Manp; and mannose was the chief component monosaccharide. NMR analyses showed that ~t-glycosidic bonds and pyranoid rings were predominant in the oligosaccharides. The MS analyses showed that lotus seed oligosaccharides consisted of three oligosaccharides of different polymerization degree, with relative molecular weights of 342, 504 and 666 Da, and corresponding molecular formulae C12H22O11, C18H32O16 and C24H42O21. Research on the effect of lotus seed oligosaccharides on the proliferation orB. adolescentis showed that they effectively promoted the production of acetic, propionic and butyric acids by B. adolescentis through fermentation, and their effect was stronger than that of fructo-, xylo- and isomalto-oligosaccharides. Lotus seed oligosaccharides have potential as a new functional probiotic and lotus seeds should be further explored and utilized as a source of oligosaccharides.展开更多
The survey＇s results showed a significant percentage of women and especially men having an above normal cholesterol. In order to help reduce excessive rate of blood cholesterol, we used a local strain of Spirulina （...The survey＇s results showed a significant percentage of women and especially men having an above normal cholesterol. In order to help reduce excessive rate of blood cholesterol, we used a local strain of Spirulina （Tamanrasset, in south of Algeria） associated with probiotic bacteria （Bf adolescentis）. Experiments in vitro showed a significant degradation of total cholesterol by the combination of Spirulina and Bf adolescentis （74.5%） after 72 hours incubation at 37 ~C. A cholesterol is added to the standard diet mice in order to increase the total cholesterol for three lots. However, the rates of total cholesterol in mice receiving lower fermented milk with Bf adolescentis enriched by dry Spirulina. This shows that the decrease of cholesterol rate is closely related to the presence of Bf adolescentis and Spirulina. In vitro and in vivo results show, however, that it is possible to obtain a natural product （Spirulina） and a pseudo-strain lactic （Bf adolescentis） and to participate in the prevention of cardiovascular disease risk factor whose hand is cholesterol.展开更多
Background The interplay between gut microbiota and tumor microenvironment(TME)in the pathogenesis of colorectal cancer(CRC)is not well explored.Here,we elucidated the functional role of Bifidobacterium adolescentis(B...Background The interplay between gut microbiota and tumor microenvironment(TME)in the pathogenesis of colorectal cancer(CRC)is not well explored.Here,we elucidated the functional role of Bifidobacterium adolescentis(B.a)on CRC and investigated its possible mechanism on the manipulation of cancer-associated fibroblasts(CAFs)in CRC.Methods Different CRC animal models and various cell line models were established to explore the function of B.a on CRC.The single-cell RNA sequencing(scRNA-seq)or flow cytometry was used to detect the cell subsets in the TME of CRC.Western blot,quantitative real-time polymerase chain reaction(qRT-PCR),or immunofluorescence staining were performed to examine the activation of Wnt signaling and growth arrest specific 1(GAS1)on CD143+CAFs.Chromatin immunoprecipitation quantitative real-time PCR(CHIP-qPCR)was performed to investigate the regulation of transcription factor 4(TCF4)on GAS1.Multi-immunofluorescence assay examined the expression level of CD143 and GAS1 on tissue microarray.Results We found that B.a abundance was significantly reduced in CRC patients from two independent cohorts and the bacteria database of GMrepo.Supplementation with B.a suppressed ApcMin/+spontaneous or AOM/DSS-induced tumorigenesis in mice.scRNA-seq revealed that B.a facilitated a subset of CD143+CAFs by inhibiting the infiltration of Th2 cells,while promoting the TNF-alpha+B cells in TME.CD143+CAFs highly expressed GAS1 and exhibited tumor suppressive effect.Mechanistically,GAS1 was activated by the Wnt/β-catenin signaling in CD143+CAFs.B.a abundance was correlated with the expression level of CD143 and GAS1.The level of CD143+CAFs predicted the better survival outcome in CRC patients.Conclusions These results highlighted that B.a induced a new subset of CD143+CAFs by Wnt signaling-regulated GAS1 to suppress tumorigenesis and provided a novel therapeutic target for probiotic-based modulation of TME in CRC.展开更多
Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Pe...Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Penicillium expansum and patulin production;however,its modes of action remain unclear.RNA-seq analysis was used to investigate the underlying antifungal mechanism of metabolites produced by B .adolescents CCFM1108.The expression of genes in the control group and three treatment groups (1/2 MIC CFS,1/4 MIC CFS,and acid treatment groups) was determined and further compared using GO and KEGG analyses.The inhibitory metabolites were found to participate in the dysfunction of the mitochondrial membrane,affecting energy metabolism,reactive oxidative species production,and patulin biosynthesis.Compared with the mixture of multiple metabolites of Bifidobacterium ,the acid mixture affected gene expression in a more significant manner by inhibiting all critical genes included in the patulin biosynthetic pathway.The changes in the patulin biosynthetic were verified by qRT-PCR in the presence of acids.Collectively,the present study provides a clear perspective on the antifungal mechanism of metabolites produced by a B.adolescents strain through transcriptome analysis and suggests that acid mixtures could be potential candidates for the postharvest control of P .expansum .展开更多
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金supported by the National Natural Science Foundation of China(32272332,32021005,31820103010)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP22006,JUSRP51501)the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘Obesity is a major focus of researchers due to its increasing prevalence and relationship with other diseases,such as cancer and cardiovascular diseases.Probiotics are active microorganisms and have been proven to alleviate obesity by modulating the microbiota.In this study,we found that oral administration of Bifidobacterium adolescentis CCFM8630 to obese mice inhibited high-fat diet(HFD)-induced changes in body weight and adipose tissue and alleviated hepatic oxidative stress.Furthermore,B.adolescentis CCFM8630 treatment primarily affected the relative abundances of the phyla Proteobacteria and Actinobacteria,and thereby decreased the production of lipopolysaccharide(LPS)and the occurrence of LPS related diseases.A high fiber intake increased the abundance of Lactobacillus and the concentrations of short chain fatty acids in obese mice,but these changes were reversed by B.adolescentis CCFM8630 treatment.In addition,targeted metabolomic analysis and microbiota relationship analysis revealed that B.adolescentis CCFM8630 treatment modified the microbiota of obese mice by promoting the conversion of tryptophan(Trp)to xanthurenic acid,kynurenic acid,tryptamine,indole-3-acetic acid,and indole-3-carboxaldehyde;facilitated the expression of interleukin-17A and the aryl hydrocarbon receptor to generate interleukin-22 in the colon;and upregulated the expression of tight junction proteins,thereby strengthening intestinal barriers.In summary,our findings suggest that the intake of B.adolescentis CCFM8630 may alleviate obesity by modulating the gut microbiota and related Trp metabolism.
基金Supported by the Natural Science Foundation of Fujian Province(No.2011J05123)Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province([2012]03)the Scientific and Technological Innovation Team Support Plan of Fujian Agriculture and Forestry University(cxtd12009)
文摘In order to study the structure of lotus （Nelumbo nucifera Gaertn） seed oligosaccharides and their effect on the proliferation ofBifidobacterium adolescentis, we extracted the oligosaccharides from seeds collected from Jianning County, China. We preliminarily characterized the groups, molecular weights, molecular formulae, component monosaccharides and glycosidic bonds using mass spectrometry （MS） and nuclear magnetic resonance （NMR） after isolation and purification. The lotus seed oligosaccharides contained glycosidic bonds Manp-（1→）, Galp-（1→）, α（1→6）-Glup and α（1→6）-Manp; and mannose was the chief component monosaccharide. NMR analyses showed that ~t-glycosidic bonds and pyranoid rings were predominant in the oligosaccharides. The MS analyses showed that lotus seed oligosaccharides consisted of three oligosaccharides of different polymerization degree, with relative molecular weights of 342, 504 and 666 Da, and corresponding molecular formulae C12H22O11, C18H32O16 and C24H42O21. Research on the effect of lotus seed oligosaccharides on the proliferation orB. adolescentis showed that they effectively promoted the production of acetic, propionic and butyric acids by B. adolescentis through fermentation, and their effect was stronger than that of fructo-, xylo- and isomalto-oligosaccharides. Lotus seed oligosaccharides have potential as a new functional probiotic and lotus seeds should be further explored and utilized as a source of oligosaccharides.
文摘The survey＇s results showed a significant percentage of women and especially men having an above normal cholesterol. In order to help reduce excessive rate of blood cholesterol, we used a local strain of Spirulina （Tamanrasset, in south of Algeria） associated with probiotic bacteria （Bf adolescentis）. Experiments in vitro showed a significant degradation of total cholesterol by the combination of Spirulina and Bf adolescentis （74.5%） after 72 hours incubation at 37 ~C. A cholesterol is added to the standard diet mice in order to increase the total cholesterol for three lots. However, the rates of total cholesterol in mice receiving lower fermented milk with Bf adolescentis enriched by dry Spirulina. This shows that the decrease of cholesterol rate is closely related to the presence of Bf adolescentis and Spirulina. In vitro and in vivo results show, however, that it is possible to obtain a natural product （Spirulina） and a pseudo-strain lactic （Bf adolescentis） and to participate in the prevention of cardiovascular disease risk factor whose hand is cholesterol.
基金National Natural Science Foundation of China,Grant/Award Numbers:82072623,82270573,82203618Zhejiang Province Natural Science Foundation,Grant/Award Number:LZ22H160002Zhejiang Province Medicine and Health Science and Technology Project,Grant/Award Numbers:2023KY722,2023KY785。
文摘Background The interplay between gut microbiota and tumor microenvironment(TME)in the pathogenesis of colorectal cancer(CRC)is not well explored.Here,we elucidated the functional role of Bifidobacterium adolescentis(B.a)on CRC and investigated its possible mechanism on the manipulation of cancer-associated fibroblasts(CAFs)in CRC.Methods Different CRC animal models and various cell line models were established to explore the function of B.a on CRC.The single-cell RNA sequencing(scRNA-seq)or flow cytometry was used to detect the cell subsets in the TME of CRC.Western blot,quantitative real-time polymerase chain reaction(qRT-PCR),or immunofluorescence staining were performed to examine the activation of Wnt signaling and growth arrest specific 1(GAS1)on CD143+CAFs.Chromatin immunoprecipitation quantitative real-time PCR(CHIP-qPCR)was performed to investigate the regulation of transcription factor 4(TCF4)on GAS1.Multi-immunofluorescence assay examined the expression level of CD143 and GAS1 on tissue microarray.Results We found that B.a abundance was significantly reduced in CRC patients from two independent cohorts and the bacteria database of GMrepo.Supplementation with B.a suppressed ApcMin/+spontaneous or AOM/DSS-induced tumorigenesis in mice.scRNA-seq revealed that B.a facilitated a subset of CD143+CAFs by inhibiting the infiltration of Th2 cells,while promoting the TNF-alpha+B cells in TME.CD143+CAFs highly expressed GAS1 and exhibited tumor suppressive effect.Mechanistically,GAS1 was activated by the Wnt/β-catenin signaling in CD143+CAFs.B.a abundance was correlated with the expression level of CD143 and GAS1.The level of CD143+CAFs predicted the better survival outcome in CRC patients.Conclusions These results highlighted that B.a induced a new subset of CD143+CAFs by Wnt signaling-regulated GAS1 to suppress tumorigenesis and provided a novel therapeutic target for probiotic-based modulation of TME in CRC.
基金supported by National Natural Science Foundation of China Key Program(31772090,32001665,31820103010,U1903205)Natural Science Foundation of Jiangsu Province(BE2021623)+1 种基金the Key Scientific and Technological Research Projects(2018AB010)BBSRC Newton Fund Joint Centre Award.
文摘Penicillium expansum is the primary postharvest pathogen that attacks fruits and vegetables and threatens human health.Bifidobacterium adolescentis CCFM1108 has been previously demonstrated to inhibit the growth of Penicillium expansum and patulin production;however,its modes of action remain unclear.RNA-seq analysis was used to investigate the underlying antifungal mechanism of metabolites produced by B .adolescents CCFM1108.The expression of genes in the control group and three treatment groups (1/2 MIC CFS,1/4 MIC CFS,and acid treatment groups) was determined and further compared using GO and KEGG analyses.The inhibitory metabolites were found to participate in the dysfunction of the mitochondrial membrane,affecting energy metabolism,reactive oxidative species production,and patulin biosynthesis.Compared with the mixture of multiple metabolites of Bifidobacterium ,the acid mixture affected gene expression in a more significant manner by inhibiting all critical genes included in the patulin biosynthetic pathway.The changes in the patulin biosynthetic were verified by qRT-PCR in the presence of acids.Collectively,the present study provides a clear perspective on the antifungal mechanism of metabolites produced by a B.adolescents strain through transcriptome analysis and suggests that acid mixtures could be potential candidates for the postharvest control of P .expansum .
