The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communicat...The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communication distance and signal strength gradually increased with the data transfer rate reducing in multiples; better communication effects were achieved in the data transfer rate of 60 kbps or 10 kbps; the active tags could be recognized accurately within 50 m, greatly improving the security of swine food safety traceability system.展开更多
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants...About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.展开更多
Lipids perform a diverse and unique array of functions in insects.Lipases are key enzymes in lipid metabolism,and their metabolic products are crucial for development and reproduction of insects.Here,a total of 110 li...Lipids perform a diverse and unique array of functions in insects.Lipases are key enzymes in lipid metabolism,and their metabolic products are crucial for development and reproduction of insects.Here,a total of 110 lipase genes were identified in the genome of Spodoptera frugiperda.Cluster analysis indicated that neutral lipases constitute the majority of lipases.Tissue expression profile analysis displayed that most lipase genes were highly expressed in the larval gut of S.frugiperda.Some lipases exhibited a diet-specific expression pattern,which implied their roles in host adaptation.Key domain analysis proved that none of the neutral lipases highly expressed in the gut has an integrated lid domain,while most lipases highly expressed in the fat body contained both the integrated lid domain andβ9 loop,indicating the activity loss of neutral lipases in guts.The assay of triacylglycerol(TAG)hydrolytic activity confirmed that the gut had the lowest activity when compared to that of fat body and epidermis.Interestingly,the opposite TAG hydrolytic activity trends across mating were observed between adult males and females,implying that lipase played different roles in the reproduction of both sexes.In conclusion,neutral lipases lost TAG hydrolytic activity in S.frugiperda guts,but retained the activity in fat body.Neutral lipases would play vital roles in many physiological processes in insects,especially in insect reproduction,which provides palpable targets for novel insecticide development to control insect population growth.展开更多
文摘The study aimed at introducing the design scheme of the active tag in 2.4 GHz frequency band for pig based on RFID technology. And through the actual circuit board test, the following results were come up： communication distance and signal strength gradually increased with the data transfer rate reducing in multiples; better communication effects were achieved in the data transfer rate of 60 kbps or 10 kbps; the active tags could be recognized accurately within 50 m, greatly improving the security of swine food safety traceability system.
基金supported by the National High Technology Research and Development Program of China (No.2002AAZ2001)the National Natural Sciences Foundation of China (No.30270758 and 30621001)
文摘About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
基金supported by the National Natural Science Foundation of China(32402373)National Key R&D Program of China(2021YFD1400700 and 2022YFD1400604).
文摘Lipids perform a diverse and unique array of functions in insects.Lipases are key enzymes in lipid metabolism,and their metabolic products are crucial for development and reproduction of insects.Here,a total of 110 lipase genes were identified in the genome of Spodoptera frugiperda.Cluster analysis indicated that neutral lipases constitute the majority of lipases.Tissue expression profile analysis displayed that most lipase genes were highly expressed in the larval gut of S.frugiperda.Some lipases exhibited a diet-specific expression pattern,which implied their roles in host adaptation.Key domain analysis proved that none of the neutral lipases highly expressed in the gut has an integrated lid domain,while most lipases highly expressed in the fat body contained both the integrated lid domain andβ9 loop,indicating the activity loss of neutral lipases in guts.The assay of triacylglycerol(TAG)hydrolytic activity confirmed that the gut had the lowest activity when compared to that of fat body and epidermis.Interestingly,the opposite TAG hydrolytic activity trends across mating were observed between adult males and females,implying that lipase played different roles in the reproduction of both sexes.In conclusion,neutral lipases lost TAG hydrolytic activity in S.frugiperda guts,but retained the activity in fat body.Neutral lipases would play vital roles in many physiological processes in insects,especially in insect reproduction,which provides palpable targets for novel insecticide development to control insect population growth.
