Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their fu...Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their functions have not yet been confirmed,especially for SULTR3 in rice.In this study,we analyzed the expression patterns,subcellular localization,and inorganic phosphate(Pi)transporter activity of SULTR3 proteins in yeast.Except for OsSULTR3.4,which localized to the plasma membrane,other OsSULTR3 members were localized to the endoplasmic reticulum(ER)membrane in rice protoplast cells.展开更多
Lead （Pb） chemical fixation is an important environmental aspect for human health. Phosphate rocks （PRs） were utilized as an adsorbent to remove Pb from aqueous solution. Raw PRs and oxalic acid-activated PRs （A...Lead （Pb） chemical fixation is an important environmental aspect for human health. Phosphate rocks （PRs） were utilized as an adsorbent to remove Pb from aqueous solution. Raw PRs and oxalic acid-activated PRs （APRs） were used to investigate the effect of chemical modification on the Pb-binding capacity in the pH range 2.0-5.0. The Pb adsorption rate of all treatments above pH 3.0 reached 90%. The Pb binding on PRs and APRs was pH-independent, except at pH 2.0 in activated treatments. The X-ray diffraction analysis confirmed that the raw PRs formed cerussite after reacting with the Pb solution, whereas the APRs formed pyromorphite. The Fourier Transform Infrared spectroscopy analysis indicated that carbonate （CO32-） in raw PRs and phosphate （PO43-） groups in APRs played an important role in the Pb-binding process. After adsorption, anomalous block-shaped particles were observed by scanning electron microscopy with energy dispersive spectroscopy. The X-ray photoelectron spectroscopy data further indicated that both chemical and physical reactions occurred during the adsorption process according to the binding energy. Because of lower solubility of pyromorphite compared to cerussite, the APRs are more effective in immobilizing Pb than that of PRs.展开更多
The phylogenetic diversity of phosphate solubilizing bacteria （PSB） distributed in P-rich soils in the Dianchi Lake drainage area of China was characterized, and the tricalcium phosphate （TCP） solubilizing activit...The phylogenetic diversity of phosphate solubilizing bacteria （PSB） distributed in P-rich soils in the Dianchi Lake drainage area of China was characterized, and the tricalcium phosphate （TCP） solubilizing activities of isolated PSB were determined. Among 1 328 bacteria isolated from 100 P-rich soil samples, 377 isolates （28.39% of the total） that exhibited TCP solubilization activity were taken as PSB. These PSB showed different abilities to solubilize TCP, with the concentrations of solubilized P in bacterial cultures varying from 33.48 to 69.63 mg L^（-1）. A total of 123 PSB isolates, with relatively high TCP solubilization activity （〉 54.00 mg L^（-1））, were submitted for restriction fragment length polymorphism （RFLP） analysis, which revealed 32 unique RFLP patterns. Based on these patterns, 62 representative isolates, one to three from each RFLP pattern, were selected for 16S rRNA sequencing. Phylogenetic analysis placed the 123 PSB into three bacterial phyla, namely Proteobacteria, Aetinobacteria and Firmicutes. Members of Proteobacteria were the dominant PSB, where 107 isolates represented by 26 RFLP patterns were associated with the genera of Burkholderia, Pseudomonas, Acinetobacter, Enterobacter, Pantoea, Serratia, Klebsiella, Leclercia, Raoultella and Cedeeea. Firmicutes were the subdominant group, in which 13 isolates were affiliated with the genera of Bacillus and Brevibacterium. The remaining 3 isolates were identified as three species of the genus Arthrobacter. This research extends the knowledge on PSB in P-rich soils and broadens the spectrum of PSB for the development of environmentally friendly biophosphate fertilizers.展开更多
In this study,hierarchical Ag/La2 O2 CO3 micro/nanostructures(MNSs)were synthesized by in situ loading Ag nanoparticles(NPs)on the surface of the La2 O2 CO3 MNSs.The prepared La2 O2 CO3 MNSs present flower-like shape ...In this study,hierarchical Ag/La2 O2 CO3 micro/nanostructures(MNSs)were synthesized by in situ loading Ag nanoparticles(NPs)on the surface of the La2 O2 CO3 MNSs.The prepared La2 O2 CO3 MNSs present flower-like shape and can be tuned by the molar ratio of La(NO3)3 and CO(NH2)2.In the molar ratio of 1:2 to 1:55,the La2 O2 CO3 MNSs mainly consist of polyhedral rods,irregular rods and irregular spindles and their size is about 10,8 and 7μm,respectively.After loading Ag NPs,the spindle-like Ag/La2 O2 CO3 MNSs were used for phosphate removal and antibacterial activity.At the initial phosphate concentration of20 mg/L,the removal rate is 59.6%.The Ag/La2 O2 CO3 MNSs have significant antibacterial activity and their MIC values for S.aureus and E.coli are 31.3 and 15.6μg/mL,respectively.The results indicate that Ag/La2 O2 CO3 MNSs may have good application prospects in open water to inhibit bacterial growth.展开更多
Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride(CaCl_2) an...Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride(CaCl_2) and a phosphate buffer solution(PBS), and a mixture of aqueous solution and organic solvent, a PBS-methanol(8:2,volume/volume) mixture(PBS-M), were performed to assess the bioavailability of chlorimuron-ethyl in soil in comparison to a battery of toxicity tests in wheat seedlings. The results indicated that the peroxidase(POD) activity in wheat leaves after 7 d of exposure was one of the sensitive biomarkers of chlorimuron-ethyl in soil.The extractability of chlorimuron-ethyl by all the 3 solvents decreased with exposure time, and the rate of decrease of the PBS-M extraction between 1 and 7 d of exposure was substantially higher than those of the aqueous solution extractions. Chlorimuron-ethyl gradually changed from a water-soluble form into a soil organic matter(SOM)-bound form in the soil. The PBS extraction correlated best with the POD activity in the leaves after 7 d of exposure.展开更多
While strong absorption is best achieved with high activator concentrations,luminescence brightness typically suffers in that case due to energy migration and concentration quenching.The teams around Xia and Zhong hav...While strong absorption is best achieved with high activator concentrations,luminescence brightness typically suffers in that case due to energy migration and concentration quenching.The teams around Xia and Zhong have reported about an aphthitalite-type Eu-activated phosphate that breaks with this paradigm and enables the sustainment of high photoluminescence quantum yields(>40%)up to concentrations as high as 70 mol%Eu.Mixing of that phosphor with a low(0.8%)and high(70%)Eu fraction results in almost natural white light with excellent color rendition and correlated color temperature that could serve as a new instructive concept for next-generation phosphor-converted white LEDs.展开更多
Background:Shuangshen granule s(SSGs) are extensively utilized for the treatment of lung cancer in China and have been reported to possess tumor-protective and anti-metastatic effects.Therefore,it is crucial to unders...Background:Shuangshen granule s(SSGs) are extensively utilized for the treatment of lung cancer in China and have been reported to possess tumor-protective and anti-metastatic effects.Therefore,it is crucial to understand the precise mechanism.Building upon the findings of our previous study,the objective of the present study was to explore the impact of S SGs on the sphingosine-1-phosphate receptor-1(S1PR1)/signal transducer and activator of transcription 3(STAT3) axis,as well as the recruitment of myeloid-derived suppressor cells(MDSCs) during the formation of the premetastatic niches(PMNs).Methods:In a mouse xenograft model utilizing Lewis lung carcinoma(LLC) cells that express green fluorescent protein(GFP),the initiation of lung metastasis was monitored every three days until day 35 following transplantation.Lung metastasis,MD SC recruitment,the expression of PMN and S1PR1/STAT3 axis biomarkers,as well as the blood levels of granulocyte-mac rophage colony-stimulating factor(GM-CSF) and transforming growth factor-β(TGF-β) were assessed in the SSG treatment and control groups.Results:The LLC cells did not reach the lung until 14-17 days following subcutaneous implantation,which was concurrent with the formation of lung PMNs.S SG significantly postponed the initiation of lung metastasis and reduced the recruitment of MDSCs to the lung PMNs.S SG also suppre ssed the S1PR1/STAT3 axis in tumor tissue s,bone marrow,and lung PMNs.Additionally,SSG suppres sed the blood levels of GM-CSF and TGF-β,as well as the PMN markers,matrix metalloproteinase-9 and versican.Conclusion:Our findings suggested that SSG suppressed the development of MD SC-mediated PMNs by inhibiting the S1PR1/STAT3 axis,consequently postponing the initiation of lung metastasis.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32130096 and 32172667)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.Y2022QC14).
文摘Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their functions have not yet been confirmed,especially for SULTR3 in rice.In this study,we analyzed the expression patterns,subcellular localization,and inorganic phosphate(Pi)transporter activity of SULTR3 proteins in yeast.Except for OsSULTR3.4,which localized to the plasma membrane,other OsSULTR3 members were localized to the endoplasmic reticulum(ER)membrane in rice protoplast cells.
基金supported by the National Natural Science Foundation of China (No. 41071165)the Special Research Fund for the Doctoral Program of Higher Education, Ministry of Education (No. 20090146110003)
文摘Lead （Pb） chemical fixation is an important environmental aspect for human health. Phosphate rocks （PRs） were utilized as an adsorbent to remove Pb from aqueous solution. Raw PRs and oxalic acid-activated PRs （APRs） were used to investigate the effect of chemical modification on the Pb-binding capacity in the pH range 2.0-5.0. The Pb adsorption rate of all treatments above pH 3.0 reached 90%. The Pb binding on PRs and APRs was pH-independent, except at pH 2.0 in activated treatments. The X-ray diffraction analysis confirmed that the raw PRs formed cerussite after reacting with the Pb solution, whereas the APRs formed pyromorphite. The Fourier Transform Infrared spectroscopy analysis indicated that carbonate （CO32-） in raw PRs and phosphate （PO43-） groups in APRs played an important role in the Pb-binding process. After adsorption, anomalous block-shaped particles were observed by scanning electron microscopy with energy dispersive spectroscopy. The X-ray photoelectron spectroscopy data further indicated that both chemical and physical reactions occurred during the adsorption process according to the binding energy. Because of lower solubility of pyromorphite compared to cerussite, the APRs are more effective in immobilizing Pb than that of PRs.
基金the National Key Sciences and Technology Program for Water Solutions, China (Nos.2009ZX07102-004 and2012ZX07102-003)the National Natural Science Foundation of China (Nos.30970100 and 31160376)+1 种基金the Zhengzhou Tobacco Research Institute of China (No.122009CZ0420)the Department of Science and Technology of Yunnan Province, China (Nos.2011FA002and 11N010905)
文摘The phylogenetic diversity of phosphate solubilizing bacteria （PSB） distributed in P-rich soils in the Dianchi Lake drainage area of China was characterized, and the tricalcium phosphate （TCP） solubilizing activities of isolated PSB were determined. Among 1 328 bacteria isolated from 100 P-rich soil samples, 377 isolates （28.39% of the total） that exhibited TCP solubilization activity were taken as PSB. These PSB showed different abilities to solubilize TCP, with the concentrations of solubilized P in bacterial cultures varying from 33.48 to 69.63 mg L^（-1）. A total of 123 PSB isolates, with relatively high TCP solubilization activity （〉 54.00 mg L^（-1））, were submitted for restriction fragment length polymorphism （RFLP） analysis, which revealed 32 unique RFLP patterns. Based on these patterns, 62 representative isolates, one to three from each RFLP pattern, were selected for 16S rRNA sequencing. Phylogenetic analysis placed the 123 PSB into three bacterial phyla, namely Proteobacteria, Aetinobacteria and Firmicutes. Members of Proteobacteria were the dominant PSB, where 107 isolates represented by 26 RFLP patterns were associated with the genera of Burkholderia, Pseudomonas, Acinetobacter, Enterobacter, Pantoea, Serratia, Klebsiella, Leclercia, Raoultella and Cedeeea. Firmicutes were the subdominant group, in which 13 isolates were affiliated with the genera of Bacillus and Brevibacterium. The remaining 3 isolates were identified as three species of the genus Arthrobacter. This research extends the knowledge on PSB in P-rich soils and broadens the spectrum of PSB for the development of environmentally friendly biophosphate fertilizers.
基金Project supported by the National Natural Science Foundation of China(21271062)。
文摘In this study,hierarchical Ag/La2 O2 CO3 micro/nanostructures(MNSs)were synthesized by in situ loading Ag nanoparticles(NPs)on the surface of the La2 O2 CO3 MNSs.The prepared La2 O2 CO3 MNSs present flower-like shape and can be tuned by the molar ratio of La(NO3)3 and CO(NH2)2.In the molar ratio of 1:2 to 1:55,the La2 O2 CO3 MNSs mainly consist of polyhedral rods,irregular rods and irregular spindles and their size is about 10,8 and 7μm,respectively.After loading Ag NPs,the spindle-like Ag/La2 O2 CO3 MNSs were used for phosphate removal and antibacterial activity.At the initial phosphate concentration of20 mg/L,the removal rate is 59.6%.The Ag/La2 O2 CO3 MNSs have significant antibacterial activity and their MIC values for S.aureus and E.coli are 31.3 and 15.6μg/mL,respectively.The results indicate that Ag/La2 O2 CO3 MNSs may have good application prospects in open water to inhibit bacterial growth.
基金supported by the National Natural Science Foundation of China(Nos.41401565,41201520 and 20807046)
文摘Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride(CaCl_2) and a phosphate buffer solution(PBS), and a mixture of aqueous solution and organic solvent, a PBS-methanol(8:2,volume/volume) mixture(PBS-M), were performed to assess the bioavailability of chlorimuron-ethyl in soil in comparison to a battery of toxicity tests in wheat seedlings. The results indicated that the peroxidase(POD) activity in wheat leaves after 7 d of exposure was one of the sensitive biomarkers of chlorimuron-ethyl in soil.The extractability of chlorimuron-ethyl by all the 3 solvents decreased with exposure time, and the rate of decrease of the PBS-M extraction between 1 and 7 d of exposure was substantially higher than those of the aqueous solution extractions. Chlorimuron-ethyl gradually changed from a water-soluble form into a soil organic matter(SOM)-bound form in the soil. The PBS extraction correlated best with the POD activity in the leaves after 7 d of exposure.
基金Open Accessfunding enabled and organized by Projekt DEAL.
文摘While strong absorption is best achieved with high activator concentrations,luminescence brightness typically suffers in that case due to energy migration and concentration quenching.The teams around Xia and Zhong have reported about an aphthitalite-type Eu-activated phosphate that breaks with this paradigm and enables the sustainment of high photoluminescence quantum yields(>40%)up to concentrations as high as 70 mol%Eu.Mixing of that phosphor with a low(0.8%)and high(70%)Eu fraction results in almost natural white light with excellent color rendition and correlated color temperature that could serve as a new instructive concept for next-generation phosphor-converted white LEDs.
基金supported by the Special Training of Scientific and Technological Talents,China Academy of Chinese Medical Sciences (grant number ZZ13-YQ-023,ZZ13-YQ-028)the National Natural Science Foundation of China(grant number 82174465,82074338,82274609,82104961,82174463)+7 种基金the Beijing Municipal Science and Technology Commission (grant number Z181100001618006)Scientificand Technological Innovation Project of China Academy of Chinese Medical Sciences (grant number CI2021A01810,CI2021A01814,CI2021A01808,CI2021B009)Traditional Chinese Medicine Innovation Team Project of the National Administration of Traditional Chinese Medicine(grant number ZYYCXTD-C-202205)Central High-Level Traditional Chinese Medicine Hospital Clinical Research and Achievement Transformation Capacity Enhancement Project-Special Project Inheriting the Academic Experienceof Renowned Traditional Chinese Medicine Experts(HLCMHPP2023012)China Academy of Chinese Medical Sciences Special Project for Research and Development of Superior Diseases,Hospital Preparations,New Drugs(ZZ15-XY-PT-01)Xu Hang Project for Cultivating Young Top Talents at Guang'anmen Hospital (CZ40908)The Fundamental Research Funds for the Central Public Welfare Research Institutes (ZZ17XRZ-023)Clinical Research Expenses of the Central High-level Hospital of Traditional Chinese Medicine (HLCMHPP2023101)
文摘Background:Shuangshen granule s(SSGs) are extensively utilized for the treatment of lung cancer in China and have been reported to possess tumor-protective and anti-metastatic effects.Therefore,it is crucial to understand the precise mechanism.Building upon the findings of our previous study,the objective of the present study was to explore the impact of S SGs on the sphingosine-1-phosphate receptor-1(S1PR1)/signal transducer and activator of transcription 3(STAT3) axis,as well as the recruitment of myeloid-derived suppressor cells(MDSCs) during the formation of the premetastatic niches(PMNs).Methods:In a mouse xenograft model utilizing Lewis lung carcinoma(LLC) cells that express green fluorescent protein(GFP),the initiation of lung metastasis was monitored every three days until day 35 following transplantation.Lung metastasis,MD SC recruitment,the expression of PMN and S1PR1/STAT3 axis biomarkers,as well as the blood levels of granulocyte-mac rophage colony-stimulating factor(GM-CSF) and transforming growth factor-β(TGF-β) were assessed in the SSG treatment and control groups.Results:The LLC cells did not reach the lung until 14-17 days following subcutaneous implantation,which was concurrent with the formation of lung PMNs.S SG significantly postponed the initiation of lung metastasis and reduced the recruitment of MDSCs to the lung PMNs.S SG also suppre ssed the S1PR1/STAT3 axis in tumor tissue s,bone marrow,and lung PMNs.Additionally,SSG suppres sed the blood levels of GM-CSF and TGF-β,as well as the PMN markers,matrix metalloproteinase-9 and versican.Conclusion:Our findings suggested that SSG suppressed the development of MD SC-mediated PMNs by inhibiting the S1PR1/STAT3 axis,consequently postponing the initiation of lung metastasis.
