Objective:The objective of this study is to explore the current situation of nurses’participant activation for self-management of low back pain,analyze its influencing factors,and provide a reference for improving th...Objective:The objective of this study is to explore the current situation of nurses’participant activation for self-management of low back pain,analyze its influencing factors,and provide a reference for improving the participant activation for self-management of low back pain.Materials and Methods:A cross-sectional study was conducted with 5,120 nurses recruited through convenience sampling from hospitals across 25 provinces and municipalities in China.Participants completed two standardized instruments:General Information Questionnaire which collected demographic characteristics and work-related factors,and the Participant Activation for Self-Management of Back Pain Questionnaire which measured activation levels across three dimensions(including self-management beliefs,self-management awareness,and knowledge related to low back pain).Results:The score of nurses’participant activation for low back pain self-management was 38.00(33.00,44.00).The results of multiple linear regression showed that nurses of different gender,age,and professional titles,whether they had administrative positions,and whether they had back pain in the past year were influencing factors of nurses’participant activation for low back pain management.Conclusion:The overall participant activation for self-management of low back pain is at a medium level.Their self-management awareness is strong,but their beliefs about management of low back pain are insufficient.Targeted intervention measures can be taken to improve their activation for management of back pain and reduce the incidence of occupational low back pain.展开更多
Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activ...Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.展开更多
Peroxymonosulfate(PMS)-assisted visible-light photocatalytic degradation of organic pollutants using graphitic carbon nitride(g-C_(3)N_(4))presents a promising and environmentally friendly approach.However,pristine g-...Peroxymonosulfate(PMS)-assisted visible-light photocatalytic degradation of organic pollutants using graphitic carbon nitride(g-C_(3)N_(4))presents a promising and environmentally friendly approach.However,pristine g-C_(3)N_(4) suffers from limited visible-light absorption and low charge-carrier mobility.In this study,a phosphorus-doped tubular carbon nitride(5P-TCN)was synthesized via a precursor self-assembly method using phosphoric acid and melamine as raw materials,eliminating the need for organic solvents or templates.The 5P-TCN catalyst demonstrated enhanced visible-light absorption,improved charge transfer capability,and a 5.25-fold increase in specific surface area(31.092 m^(2)/g),which provided abundant active sites to efficiently drive the PMS-assisted photocatalytic reaction.The 5P-TCN/vis/PMS system exhibited exceptional degradation performance for organic pollutants across a broad pH range(3–9),achieving over 92%degradation of Rhodamine B(RhB)within 15 min.Notably,the system retained>98%RhB degradation efficiency after three consecutive operational cycles,demonstrating robust operational stability and reusability.Moreover,key parameters influencing,active radi-cals,degradation pathways,and potential mechanisms for RhB degradation were systematically investigated.This work proposes a green and cost-effective strategy for developing high-efficiency photocatalysts,while demon-strating the exceptional capability of a PMS-assisted photocatalytic system for rapid degradation of RhB.展开更多
The functional properties of glasses are governed by their formation history and the complex relaxation processes they undergo.However,under extreme conditions,glass behaviors are still elusive.In this study,we employ...The functional properties of glasses are governed by their formation history and the complex relaxation processes they undergo.However,under extreme conditions,glass behaviors are still elusive.In this study,we employ simulations with varied protocols to evaluate the effectiveness of different descriptors in predicting mechanical properties across both low-and high-pressure regimes.Our findings demonstrate that conventional structural and configurational descriptors fail to correlate with the mechanical response following pressure release,whereas the activation energy descriptor exhibits robust linearity with shear modulus after correcting for pressure effects.Notably,the soft mode parameter emerges as an ideal and computationally efficient alternative for capturing this mechanical behavior.These findings provide critical insights into the influence of pressure on glassy properties,integrating the distinct features of compressed glasses into a unified theoretical framework.展开更多
Objectives Physical activity during pregnancy benefits both women and their fetuses;however,the prevalence of physical inactivity is high among pregnant women in the first trimester.This study aimed to examine physica...Objectives Physical activity during pregnancy benefits both women and their fetuses;however,the prevalence of physical inactivity is high among pregnant women in the first trimester.This study aimed to examine physical activity patterns among pregnant Chinese women in the first trimester and clarify the characteristics associated with physical inactivity in the first trimester.Methods This cross-sectional study was conducted at three Class-A tertiary hospitals in Guangzhou,city,China,from December 2021 to June 2022.A total of 1,283 pregnant women were enrolled.The Pregnancy Physical Activity Questionnaire and a sociodemographic and obstetric data sheet collected information in the first trimester.Results The prevalence of physical inactivity among Chinese pregnant women was 51%.Pregnant women spent the most time in the domains of household/caregiving activities(median 38.25 h/week)and sedentary activities(median 54.25 h/week).Characteristics of pregnant women who were more likely to be physically inactive included younger age(OR:1.413,95%CI:1.291,1.767;P=0.002),unmarried(OR:2.976,95%CI:1.573,5.630;P=0.001),lower monthly income(OR:1.945,95%CI:1.535,2.464;P=0.001),self-paid medical care(OR:1.472,95%CI:1.140,1.900;P=0.003),nulliparous(OR:3.169,95%CI:2.504,4.009;P=0.001),inadequate knowledge about physical activity during pregnancy(OR:1.797,95%CI:1.432,2.255;P=0.001),husbands with no physical activity habits(OR:1.353,95%CI:1.081,1.693;P=0.008),and no prepregnancy physical activity habits(OR:1.832,95%CI:1.433,2.340;P=0.001).Conclusions Physical inactivity was prevalent among pregnant women in the first trimester.Promoting physical activity could be incorporated into routine antenatal care.Healthcare professionals should assess and monitor the physical activity of pregnant women and provide consultations on physical activity during their first trimester,particularly for pregnant women with characteristics associated with physical inactivity.展开更多
BACKGROUND Diamine oxidase(DAO)is secreted by epithelial cells in the intestinal villi,and its serum levels are elevated after intestinal mucosal damage.d-lactate(D-LA)is a gut microbial metabolite that can enter the ...BACKGROUND Diamine oxidase(DAO)is secreted by epithelial cells in the intestinal villi,and its serum levels are elevated after intestinal mucosal damage.d-lactate(D-LA)is a gut microbial metabolite that can enter the systemic circulation if intestinal barrier function is impaired.Both DAO and D-LA are serum markers of small bowel mucosal integrity,and can be valuable biomarkers of intestinal barrier damage in inflammatory bowel disease(IBD).Intestinal barrier dysfunction was recently found to contribute to psychological symptoms in IBD patients.However,the correlations among DAO,D-LA,psychological symptoms,and disease activity in IBD remain unexplored.AIM To explore the correlations between serum markers of intestinal barrier dysfunction and psychological symptoms in IBD.METHODS We enrolled of 126 participants in this study.Psychological symptom questionnaires(depression,patient health questionnaire-9;anxiety,generalized anxiety disorder-7;and stress,perceived stress scale)and a quality of life(QOL)questionnaire(IBD questionnaire 32)were collected at the baseline.Serum DAO and D-LA levels were measured to assess intestinal barrier integrity.Receiver operating characteristic(ROC)curves were used to identify candidate markers of psychological symptoms and disease activity in IBD patients.Logistic regression was applied,with DAO as an independent variable for predicting psychological symptoms in IBD.RESULTS Serum DAO levels were significantly higher in IBD patients with moderate-to-severe psychological symptoms than in patients with mild or no psychological symptoms.DAO was positively correlated with depression and negatively correlated with QOL in IBD patients.ROC curves revealed that DAO was independently associated with psychological symptoms and clinical activity in patients with IBD.Additionally,logistic regression analysis revealed that each 1-ng/mL increase in DAO levels was significantly associated with an increased risk of psychological symptoms in IBD patients(OR:1.019,95%CI:1.002-1.037).These results highlight the potential of DAO as a novel biomarker for both depression and disease activity in IBD patients.CONCLUSION This study indicates that DAO may be associated with depression and disease activity in IBD patients;however,prospective studies are required to validate its causal relationship.展开更多
Based on a formula used to calculate the activation cross-section sum of two reactions producing a sort of nuclide with a target including two isotopes, the related problems in some references have been analyzed and d...Based on a formula used to calculate the activation cross-section sum of two reactions producing a sort of nuclide with a target including two isotopes, the related problems in some references have been analyzed and discussed. It is pointed out that the calculation methods of the cross-section sum of two reactions producing the same radioactive nuclide for two isotopes in some references are improper and usually it is impossible to obtain the correct cross-section sum of two reactions producing the same radioactive nuclide for two isotopes in the case of using natural samples. At the same time, the related concepts are clarified and the correct processing method and representation are given. The comparison with the experimental results show that the theoretical analysis results are right.展开更多
The cross-sections for 46Ti(n,2n)45Ti,46Ti(n,p)46m+gSc+47Ti(n,d*)46m+gSc,46Ti(n,p)46m+gSc,47Ti(n,p)47Sc+48Ti(n,d*)47Sc,47Ti(n,p)47Sc,48Ti(n,p)48Sc+49Ti(n,d*)48Sc,48Ti(n,p)48Sc,and 50Ti(n,α)47Ca reactions were investi...The cross-sections for 46Ti(n,2n)45Ti,46Ti(n,p)46m+gSc+47Ti(n,d*)46m+gSc,46Ti(n,p)46m+gSc,47Ti(n,p)47Sc+48Ti(n,d*)47Sc,47Ti(n,p)47Sc,48Ti(n,p)48Sc+49Ti(n,d*)48Sc,48Ti(n,p)48Sc,and 50Ti(n,α)47Ca reactions were investigated around neutron energies of 13.5–14.8 MeV by means of the activation technique.Fast neutrons were produced by the 3H(d,n)4He reaction.Neutron energies from different directions in the measurements were obtained in advance using the method of cross-section ratios for 90Zr(n,2n)89m+gZr and 93Nb(n,2n)92mNb reactions.The results obtained are analyzed and compared with the experimental data provided by the literature and verified nuclear data in the JEFF-3.3,CENDL-3.1,ENDF/B-VIII.0 libraries,as well as results calculated by Talys-1.9 code.展开更多
Photoneutron cross-section(PNCS)data are important in various current and emerging applications.Although a few sophis-ticated methods have been developed,there is still an urgent need to study the PNCS data.In this st...Photoneutron cross-section(PNCS)data are important in various current and emerging applications.Although a few sophis-ticated methods have been developed,there is still an urgent need to study the PNCS data.In this study,we propose the extraction of PNCS distributions using a combination of gamma activation and reaction yield ratio methods.To verify the validity of the proposed extraction method,experiments for generating^(62,64)Cu and^(85m,87m)Sr isotopes via laser-induced pho-toneutron reactions were performed,and the reaction yields of these isotopes were obtained.Using the proposed extraction method,the PNCS distributions of^(63)Cu and^(86)Sr isotopes(leading to^(85m)Sr isotope production)were successfully extracted.These extracted PNCS distributions were benchmarked against available PNCS data or TALYS calculations,demonstrating the validity of the proposed extraction method.Potential applications for predicting the PNCS distributions of the 30 iso-topes are further introduced.We conclude that the proposed extraction method is an effective complement to the available sophisticated methods for measuring and evaluating PNCS data.展开更多
In this study, the activation cross-sections were measured for ^(232)Th(n,2n)^(231)Th reactions at neutron energies of 14.1 and 14.8 MeV, which were produced by a neutron generator through a T(d,n)~4He reaction. Induc...In this study, the activation cross-sections were measured for ^(232)Th(n,2n)^(231)Th reactions at neutron energies of 14.1 and 14.8 MeV, which were produced by a neutron generator through a T(d,n)~4He reaction. Induced gamma-ray activities were measured using a low background gamma ray spectrometer equipped with a high resolution HPGe detector. In the cross-section calculations, corrections were made regarding the effects of gamma-ray attenuation, dead-time, fluctuation of the neutron flux, and low energy neutrons. The measured cross-sections were compared with the literature data, evaluation data(ENDF-B/VII.1, JENDL-4.0 and CENDL-3.1), and the results of the model calculation(TALYS1.6).展开更多
Objective The relationship between non-high-density lipoprotein(NHDL)cholesterol to high-density lipoprotein cholesterol(HDL-C)ratio(NHHR)and stoke remains unknown.This study aimed to evaluate the association between ...Objective The relationship between non-high-density lipoprotein(NHDL)cholesterol to high-density lipoprotein cholesterol(HDL-C)ratio(NHHR)and stoke remains unknown.This study aimed to evaluate the association between the adult NHHR and stroke occurrence in the United States of America(USA).Methods To clarify the relationship between the NHHR and stroke risk,this study used a multivariable logistic regression model and a restricted cubic spline(RCS)model to investigate the association between the NHHR and stroke,and data from the National Health and Nutrition Examination Survey(NHANES)from 2005 to 2018.Subgroup and sensitivity analyses were conducted to test the robustness of the results.Results This study included 29,928 adult participants,of which 1,165 participants had a history of stroke.Logistic regression analysis of variables demonstrated a positive association between NHHR and stroke(OR 1.24,95%CI:1.03-1.50,P=0.026).Compared with the lowest reference group of NHHR,participants in the second,third,and fourth quartile had a significantly increased risk of stroke after full adjustments(OR:1.35,95%CI:1.08-1.69)(OR:1.83,95%CI:1.42-2.36)(OR:2.04,95%CI:1.50-2.79).In the total population,a nonlinear dose-response relationship was observed between the NHHR and stroke risk(P non-linearity=0.002).This association remained significant in several subgroup analyses.Further investigation of the NHHR may enhance our understanding of stroke prevention and treatment.Conclusion Our findings suggest a positive correlation between the NHHR and an increased prevalence of stroke,potentially serving as a novel predictive factor for stroke.Timely intervention and management of the NHHR may effectively mitigate stroke occurrence.Prospective studies are required to validate this association and further explore the underlying biological mechanisms.展开更多
Objective To assess the test-retest reliability and criterion validity of the Simplified Chinese-character version of the International Physical Activity Questionnaire Long form （IPAC）.-L） in urban community-dwelli...Objective To assess the test-retest reliability and criterion validity of the Simplified Chinese-character version of the International Physical Activity Questionnaire Long form （IPAC）.-L） in urban community-dwelling adults in Hanghzou, China. Methods A total of 158 eligible participants aged 25-59 years from 6 neighbourhoods in two central districts of Hangzhou completed the IPAQ-L questionnaire twice within a 7-day interval. Half of the subjects wore pedometers during the first 7 days. Test-retest reliability was examined by comparing the first （Day 1） and the second （Day 9） survey of IPAQ-L Criterion validity was assessed by comparing IPAO,-L with pedometer data. Results Modest to good test-retest reliability was found with intraclass correlation coefficients of 0.67 for total PA, 0.37 to 0.73 for specific dimensions, and 0.56 to 0.71 for different intensities of PA. Total PA measured by IPAO.-L was moderately correlated with exercise levels （partial r = 0.27, P = 0.020） and walking distance （partial r = 0.31, P = 0.007）, which were measured by a pedometer, after adjusting for gender, age, educational attainment and employment status. Conclusion Our results indicate that the IPAO.-L is a reliable and validated measure for assessing physica activity levels in this population and possibly the adult population in other mainland Chinese cities.展开更多
Background:The ankle-foot injuries are among the war-related injuries that cause many serious secondary problems for a lifetime.This nationwide study aimed to assess health-related quality of life and the ability to p...Background:The ankle-foot injuries are among the war-related injuries that cause many serious secondary problems for a lifetime.This nationwide study aimed to assess health-related quality of life and the ability to perform activities of daily living in veterans with ankle-foot injuries due to the Iran-Iraq war.Methods:A total of 1079 veterans with ankle-foot injuries were enrolled in a cross-sectional study from 2014 to 2016.Demographic characteristics,including age,gender,marital status,disability percent,educational level,employment and additional injuries,were collected.The ability to perform daily activities was assessed using the Barthel activities of daily living(ADL)and Lawton instrumental activities of daily living(IADL)Indexes.Physical and mental health-related quality of life(HRQOL)data were measured via the SF-36 subscales.The data were compared with those of bilateral lower limb amputees(BLLAs)and of the general Iranian population.Statistical analyses,including Pearson’s correlation coefficient,one-sample t-test and analysis of variance(ANOVA),were performed using SPSS16.0.A multiple linear regression model was used to determine the contribution of independent variables to the Physical Component Summary(PCS)and Mental Component Summary(MCS).Results:The highest and lowest scores were observed for mental health(48.93±20.69)and bodily pain(28.16±21.74),respectively.The mean scores of veterans with ankle-foot injuries on the SF-36 were significantly lower on all eight measures than those of the general Iranian male population and of the bilateral lower limb amputees(P<0.001).The mean scores of ADLs and IADLs were 83.9±16.3 and 5.3±2.0,respectively.The higher dependency in ADLs(P<0.001)and IADLs(P<0.001),the higher disability rate(P<0.001)and additional injury(P<0.001)were significant determinants of the PCS.ADL(P<0.001)and IADL(P<0.001)limitations,additional injury(P<0.001),history of hospitalization in the year preceding the study(P=0.007)and employment(P=0.001)were reported as determinants of the MCS.Conclusion:The results strongly suggest that veterans with ankle-foot injuries suffer from critically poor healthrelated quality of life.The main predicting factors of HRQOL were the disability to perform ADLs/IADLs,suffering two or more injuries,a history of hospitalization in the year preceding the study and unemployment.展开更多
The umbilical,a key component in offshore energy extraction,plays a vital role in ensuring the stable operation of the entire production system.The extensive variety of cross-sectional components creates highly comple...The umbilical,a key component in offshore energy extraction,plays a vital role in ensuring the stable operation of the entire production system.The extensive variety of cross-sectional components creates highly complex layout combinations.Furthermore,due to constraints in component quantity and geometry within the cross-sectional layout,filler bodies must be incorporated to maintain cross-section performance.Conventional design approaches based on manual experience suffer from inefficiency,high variability,and difficulties in quantification.This paper presents a multi-level automatic filling optimization design method for umbilical cross-sectional layouts to address these limitations.Initially,the research establishes a multi-objective optimization model that considers compactness,balance,and wear resistance of the cross-section,employing an enhanced genetic algorithm to achieve a near-optimal layout.Subsequently,the study implements an image processing-based vacancy detection technique to accurately identify cross-sectional gaps.To manage the variability and diversity of these vacant regions,the research introduces a multi-level filling method that strategically selects and places filler bodies of varying dimensions,overcoming the constraints of uniform-size fillers.Additionally,the method incorporates a hierarchical strategy that subdivides the complex cross-section into multiple layers,enabling layer-by-layer optimization and filling.This approach reduces manufac-turing equipment requirements while ensuring practical production process feasibility.The methodology is validated through a specific umbilical case study.The results demonstrate improvements in compactness,balance,and wear resistance compared with the initial cross-section,offering novel insights and valuable references for filler design in umbilical cross-sections.展开更多
Objective:Postpartum nutrition plays a critical role in maternal recovery and long-term health.However,the nutritional status of working mothers in the postpartum period remains understudied.This study aimed to assess...Objective:Postpartum nutrition plays a critical role in maternal recovery and long-term health.However,the nutritional status of working mothers in the postpartum period remains understudied.This study aimed to assess the dietary quality of postpartum women in urban Beijing,identify occupational-related factors influencing their diet,and explore potential interventions to improve maternal nutrition during the postpartum period.Methods:In this cross-sectional analysis,554 women one year after delivery were recruited from ten community health centers.Sociodemographic,occupational and postpartum care variables were collected via questionnaire.Dietary intake over the preceding year was assessed using a food frequency questionnaire.The modified dietary balance index for postpartum women were used for dietary quality assessment.Results:The study revealed severe dietary imbalances among postpartum women,characterized by excessive consumption of cereals,eggs,and meats,while their intake of vegetables,fruits,and dairy products was inadequate.According to dietary balance index for postpartum women,66.25%of mothers showed varying degrees of excessive intake.45.31%of mothers experienced varying levels of insufficient intake,with only 19.86%of participants having a relatively balanced diet.Occupational differences were observed,with women in the commercial employment group showing higher levels of excessive food intake.The analysis of influencing factors showed that family monthly income,maternity leave,and postpartum care significantly affected the dietary quality.Conclusions:Postpartum women in Beijing experience widespread dietary imbalances,with both excesses and deficiencies.Occupational context and related factors significantly shape diet quality.These findings highlight the need for targeted nutritional interventions tailored to the specific challenges of different occupational groups.展开更多
OBJECTIVE:To investigate the difference in gut microbiota between population with damp-heat constitution(DHC)and balanced constitution(BC).METHODS:A multi-centered cross-sectional casecontrol study was conducted,which...OBJECTIVE:To investigate the difference in gut microbiota between population with damp-heat constitution(DHC)and balanced constitution(BC).METHODS:A multi-centered cross-sectional casecontrol study was conducted,which included 249 participants with damp-heat constitution or balanced constitution.Baseline information of participants was collected,and stool samples were collected for gut microbiota analysis.Principal coordinate analysis,linear discriminant analysis effect size analysis,receiver operating characteristic,random forest model,and phylogenetic investigation of communities by reconstruction of unobserved states methods were used to reveal the relationship between gut microbiota and the damp-heat constitution.RESULTS:Compared to those in the BC group,the richness and diversity of the microbiota,specifically those of several short-chain fatty acid producing genera such as Barnesiella,Coprobacter,and Butyricimonas,were significantly decreased in the DHC group.Regarding biological functions,flavonoid biosynthesis,propanoate metabolism,and nucleotide sugar metabolism were suppressed,while arachidonic acid metabolism and glutathione metabolism were enriched in the DHC group.Finally,a classifier based on the microbiota was constructed to discriminate between the DHC and BC populations.CONCLUSION:The gut microbiota of the DHC population exhibits significantly reduced diversity and is closely related to inflammation,metabolic disorders,and liver steatosis,which is consistent with clinical observations,thus serving as a potential diagnostic tool for traditional Chinese medicine constitution discrimination.展开更多
To investigate the effects of the maximum principal stress direction(θ)and cross-section shape on the failure characteristics of sandstone,true-triaxial compression experiments were conducted using cubic samples with...To investigate the effects of the maximum principal stress direction(θ)and cross-section shape on the failure characteristics of sandstone,true-triaxial compression experiments were conducted using cubic samples with rectangular,circular,and D-shaped holes.Asθincreases from 0°to 60°in the rectangular hole,the left failure location shifts from the left corner to the left sidewall,the left corner,and then the floor,while the right failure location shifts from the right corner to the right sidewall,right roof corner,and then the roof.Furthermore,the initial failure vertical stress first decreases and then increases.In comparison,the failure severity in the rectangular hole decreases for variousθvalues as 30°>45°>60°>0°.With increasingθ,the fractal dimension(D)of rock slices first increases and then decreases.For the rectangular and D-shaped holes,whenθ=0°,30°,and 90°,D for the rectangular hole is less than that of the D-shaped hole.Whenθ=45°and 60°,D for the rectangular hole is greater than that of the D-shaped hole.Theoretical analysis indicates that the stress concentration at the rectangular and D-shaped corners is greater than the other areas.The failure location rotates with the rotation ofθ,and the failure occurs on the side with a high concentration of compressive stress,while the side with the tensile and compressive stresses remains relatively stable.Therefore,the fundamental reason for the rotation of failure location is the rotation of stress concentration,and the external influencing factor is the rotation ofθ.展开更多
Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproducti...Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.展开更多
Calcineurin(CN)is a calcium-and calmodulindependent serine/threonine that has been studied in many model organisms including yeast,filamentous fungi,plants,and mammals.Its biological functions range from ion homeostas...Calcineurin(CN)is a calcium-and calmodulindependent serine/threonine that has been studied in many model organisms including yeast,filamentous fungi,plants,and mammals.Its biological functions range from ion homeostasis and virulence in lower eukaryotes to T-cell activation in humans by human nuclear factors of activated T-cells.CN is a heterodimeric protein consisting of a catalytic subunit,calcineurin A(Cna1p),which contains an active site with a dinuclear metal center,and a regulatory Ca^(2+) binding subunit called calcineurin B(Cnb1p)required to activate Cna1p.The calcineurin B subunit has been highly conserved through evolution:For example,the mammalian calcineurin B shows 54%identity with calcineurin B from Saccharomyces cerevisiae.展开更多
The objective of this study was to characterize sympathetic nervous system （SNS） activity in girls with adolescent idiopathic scoliosis （AIS） and investigate the correlation of such activity with body weight and b...The objective of this study was to characterize sympathetic nervous system （SNS） activity in girls with adolescent idiopathic scoliosis （AIS） and investigate the correlation of such activity with body weight and bone mass. Fifty-eight girls with AIS and 21 healthy controls were recruited. Abnormal growth pattern （lower weight, lower body mass index （BMI）, and longer arm span） and significantly increased adrenaline （AD） level, norepinephrine （NE） level, and low-frequency （LF） were found in girls with AIS. Circulating AD level, NE level, and LF power were negatively correlated with body weight and BMI but significantly correlated with bone quality.展开更多
文摘Objective:The objective of this study is to explore the current situation of nurses’participant activation for self-management of low back pain,analyze its influencing factors,and provide a reference for improving the participant activation for self-management of low back pain.Materials and Methods:A cross-sectional study was conducted with 5,120 nurses recruited through convenience sampling from hospitals across 25 provinces and municipalities in China.Participants completed two standardized instruments:General Information Questionnaire which collected demographic characteristics and work-related factors,and the Participant Activation for Self-Management of Back Pain Questionnaire which measured activation levels across three dimensions(including self-management beliefs,self-management awareness,and knowledge related to low back pain).Results:The score of nurses’participant activation for low back pain self-management was 38.00(33.00,44.00).The results of multiple linear regression showed that nurses of different gender,age,and professional titles,whether they had administrative positions,and whether they had back pain in the past year were influencing factors of nurses’participant activation for low back pain management.Conclusion:The overall participant activation for self-management of low back pain is at a medium level.Their self-management awareness is strong,but their beliefs about management of low back pain are insufficient.Targeted intervention measures can be taken to improve their activation for management of back pain and reduce the incidence of occupational low back pain.
文摘Rationale:This case report describes a couple with recurrent fertilization failure despite undergoing multiple cycles of intracytoplasmic sperm injection(ICSI).The principal clinical concern was suspected oocyte activation deficiency(OAD),in which fertilization is impeded due to the oocyte’s inability to initiate embryogenesis,commonly attributed to inadequate intracellular calcium(Ca^(2+))release following sperm injection.Patient concerns:The couple repeatedly experienced complete or near-complete fertilization failure in previous ICSI cycles,raising suspicion of an underlying oocyte activation defect.Diagnosis:Based on the repeated absence of fertilization post-ICSI and clinical history,a diagnosis of suspected OAD leading to recurrent ICSI fertilization failure was considered.Interventions:Artificial oocyte activation(AOA)using the calcium ionophore A23187 was performed.After ICSI,unfertilized oocytes were exposed to the ionophore to induce Ca^(2+)influx,simulating physiological calcium oscillations essential for oocyte activation.The efficacy of intervention was evaluated through subsequent embryonic development,morphological grading,and chromosomal integrity.Outcomes:Following AOA treatment,successful oocyte activation occurred,resulting in the formation of high-grade embryos with normal developmental progression.Chromosomal analysis revealed no detectable abnormalities,indicating genomic stability.Lessons:Calcium ionophore–mediated AOA may serve as an effective adjunct in cases of recurrent ICSI failure attributed to OAD.This case highlights the importance of individualized therapeutic strategies in assisted reproduction;however,further research is needed to refine protocols,validate broader clinical efficacy,and assess long-term safety,including potential epigenetic risks.
文摘Peroxymonosulfate(PMS)-assisted visible-light photocatalytic degradation of organic pollutants using graphitic carbon nitride(g-C_(3)N_(4))presents a promising and environmentally friendly approach.However,pristine g-C_(3)N_(4) suffers from limited visible-light absorption and low charge-carrier mobility.In this study,a phosphorus-doped tubular carbon nitride(5P-TCN)was synthesized via a precursor self-assembly method using phosphoric acid and melamine as raw materials,eliminating the need for organic solvents or templates.The 5P-TCN catalyst demonstrated enhanced visible-light absorption,improved charge transfer capability,and a 5.25-fold increase in specific surface area(31.092 m^(2)/g),which provided abundant active sites to efficiently drive the PMS-assisted photocatalytic reaction.The 5P-TCN/vis/PMS system exhibited exceptional degradation performance for organic pollutants across a broad pH range(3–9),achieving over 92%degradation of Rhodamine B(RhB)within 15 min.Notably,the system retained>98%RhB degradation efficiency after three consecutive operational cycles,demonstrating robust operational stability and reusability.Moreover,key parameters influencing,active radi-cals,degradation pathways,and potential mechanisms for RhB degradation were systematically investigated.This work proposes a green and cost-effective strategy for developing high-efficiency photocatalysts,while demon-strating the exceptional capability of a PMS-assisted photocatalytic system for rapid degradation of RhB.
基金supported by the National Natural Science Foundation of China (Grant Nos.T2325004 and 52161160330)the National Natural Science Foundation of China (Grants No.12504233)+2 种基金Advanced MaterialsNational Science and Technology Major Project (Grant No.2024ZD0606900)the Talent Hub for “AI+New Materials” Basic Researchthe Key Research and Development Program of Ningbo (Grant No.2025Z088)。
文摘The functional properties of glasses are governed by their formation history and the complex relaxation processes they undergo.However,under extreme conditions,glass behaviors are still elusive.In this study,we employ simulations with varied protocols to evaluate the effectiveness of different descriptors in predicting mechanical properties across both low-and high-pressure regimes.Our findings demonstrate that conventional structural and configurational descriptors fail to correlate with the mechanical response following pressure release,whereas the activation energy descriptor exhibits robust linearity with shear modulus after correcting for pressure effects.Notably,the soft mode parameter emerges as an ideal and computationally efficient alternative for capturing this mechanical behavior.These findings provide critical insights into the influence of pressure on glassy properties,integrating the distinct features of compressed glasses into a unified theoretical framework.
基金funded by the National Natural Science Foundation of China(Grant 72174216)。
文摘Objectives Physical activity during pregnancy benefits both women and their fetuses;however,the prevalence of physical inactivity is high among pregnant women in the first trimester.This study aimed to examine physical activity patterns among pregnant Chinese women in the first trimester and clarify the characteristics associated with physical inactivity in the first trimester.Methods This cross-sectional study was conducted at three Class-A tertiary hospitals in Guangzhou,city,China,from December 2021 to June 2022.A total of 1,283 pregnant women were enrolled.The Pregnancy Physical Activity Questionnaire and a sociodemographic and obstetric data sheet collected information in the first trimester.Results The prevalence of physical inactivity among Chinese pregnant women was 51%.Pregnant women spent the most time in the domains of household/caregiving activities(median 38.25 h/week)and sedentary activities(median 54.25 h/week).Characteristics of pregnant women who were more likely to be physically inactive included younger age(OR:1.413,95%CI:1.291,1.767;P=0.002),unmarried(OR:2.976,95%CI:1.573,5.630;P=0.001),lower monthly income(OR:1.945,95%CI:1.535,2.464;P=0.001),self-paid medical care(OR:1.472,95%CI:1.140,1.900;P=0.003),nulliparous(OR:3.169,95%CI:2.504,4.009;P=0.001),inadequate knowledge about physical activity during pregnancy(OR:1.797,95%CI:1.432,2.255;P=0.001),husbands with no physical activity habits(OR:1.353,95%CI:1.081,1.693;P=0.008),and no prepregnancy physical activity habits(OR:1.832,95%CI:1.433,2.340;P=0.001).Conclusions Physical inactivity was prevalent among pregnant women in the first trimester.Promoting physical activity could be incorporated into routine antenatal care.Healthcare professionals should assess and monitor the physical activity of pregnant women and provide consultations on physical activity during their first trimester,particularly for pregnant women with characteristics associated with physical inactivity.
基金Supported by National Natural Science Foundation of China,No.82270581 and No.82270546.
文摘BACKGROUND Diamine oxidase(DAO)is secreted by epithelial cells in the intestinal villi,and its serum levels are elevated after intestinal mucosal damage.d-lactate(D-LA)is a gut microbial metabolite that can enter the systemic circulation if intestinal barrier function is impaired.Both DAO and D-LA are serum markers of small bowel mucosal integrity,and can be valuable biomarkers of intestinal barrier damage in inflammatory bowel disease(IBD).Intestinal barrier dysfunction was recently found to contribute to psychological symptoms in IBD patients.However,the correlations among DAO,D-LA,psychological symptoms,and disease activity in IBD remain unexplored.AIM To explore the correlations between serum markers of intestinal barrier dysfunction and psychological symptoms in IBD.METHODS We enrolled of 126 participants in this study.Psychological symptom questionnaires(depression,patient health questionnaire-9;anxiety,generalized anxiety disorder-7;and stress,perceived stress scale)and a quality of life(QOL)questionnaire(IBD questionnaire 32)were collected at the baseline.Serum DAO and D-LA levels were measured to assess intestinal barrier integrity.Receiver operating characteristic(ROC)curves were used to identify candidate markers of psychological symptoms and disease activity in IBD patients.Logistic regression was applied,with DAO as an independent variable for predicting psychological symptoms in IBD.RESULTS Serum DAO levels were significantly higher in IBD patients with moderate-to-severe psychological symptoms than in patients with mild or no psychological symptoms.DAO was positively correlated with depression and negatively correlated with QOL in IBD patients.ROC curves revealed that DAO was independently associated with psychological symptoms and clinical activity in patients with IBD.Additionally,logistic regression analysis revealed that each 1-ng/mL increase in DAO levels was significantly associated with an increased risk of psychological symptoms in IBD patients(OR:1.019,95%CI:1.002-1.037).These results highlight the potential of DAO as a novel biomarker for both depression and disease activity in IBD patients.CONCLUSION This study indicates that DAO may be associated with depression and disease activity in IBD patients;however,prospective studies are required to validate its causal relationship.
基金Supported by Program for Science & Technology Innovation Talents of Universities of Henan Province, China (2008 HASTIT032)Research Program for Basic & Forefront Technology of Henan Province, China (132300410302, 102300410039)Scientific Research Start up Outlay of High-Position Talent at Pingdingshan University in Henan Province, China
文摘Based on a formula used to calculate the activation cross-section sum of two reactions producing a sort of nuclide with a target including two isotopes, the related problems in some references have been analyzed and discussed. It is pointed out that the calculation methods of the cross-section sum of two reactions producing the same radioactive nuclide for two isotopes in some references are improper and usually it is impossible to obtain the correct cross-section sum of two reactions producing the same radioactive nuclide for two isotopes in the case of using natural samples. At the same time, the related concepts are clarified and the correct processing method and representation are given. The comparison with the experimental results show that the theoretical analysis results are right.
基金Supported by the National Natural Science Foundation of China(11575090,11605099)
文摘The cross-sections for 46Ti(n,2n)45Ti,46Ti(n,p)46m+gSc+47Ti(n,d*)46m+gSc,46Ti(n,p)46m+gSc,47Ti(n,p)47Sc+48Ti(n,d*)47Sc,47Ti(n,p)47Sc,48Ti(n,p)48Sc+49Ti(n,d*)48Sc,48Ti(n,p)48Sc,and 50Ti(n,α)47Ca reactions were investigated around neutron energies of 13.5–14.8 MeV by means of the activation technique.Fast neutrons were produced by the 3H(d,n)4He reaction.Neutron energies from different directions in the measurements were obtained in advance using the method of cross-section ratios for 90Zr(n,2n)89m+gZr and 93Nb(n,2n)92mNb reactions.The results obtained are analyzed and compared with the experimental data provided by the literature and verified nuclear data in the JEFF-3.3,CENDL-3.1,ENDF/B-VIII.0 libraries,as well as results calculated by Talys-1.9 code.
基金This work was supported by the National Key R&D Program of China(No.2022YFA1603300)the National Natural Science Foundation of China(Nos.U2230133)+2 种基金the Independent Research Project of the Key Laboratory of Plasma Physics,CAEP(No.JCKYS2021212009)the Open Fund of the Key Laboratory of Nuclear Data,CIAE(No.JCKY2022201C152)Hengyang Municipal Science and Technology Project(No.202150054076).
文摘Photoneutron cross-section(PNCS)data are important in various current and emerging applications.Although a few sophis-ticated methods have been developed,there is still an urgent need to study the PNCS data.In this study,we propose the extraction of PNCS distributions using a combination of gamma activation and reaction yield ratio methods.To verify the validity of the proposed extraction method,experiments for generating^(62,64)Cu and^(85m,87m)Sr isotopes via laser-induced pho-toneutron reactions were performed,and the reaction yields of these isotopes were obtained.Using the proposed extraction method,the PNCS distributions of^(63)Cu and^(86)Sr isotopes(leading to^(85m)Sr isotope production)were successfully extracted.These extracted PNCS distributions were benchmarked against available PNCS data or TALYS calculations,demonstrating the validity of the proposed extraction method.Potential applications for predicting the PNCS distributions of the 30 iso-topes are further introduced.We conclude that the proposed extraction method is an effective complement to the available sophisticated methods for measuring and evaluating PNCS data.
基金Supported by National Natural Science Foundation of China(No.11205076)
文摘In this study, the activation cross-sections were measured for ^(232)Th(n,2n)^(231)Th reactions at neutron energies of 14.1 and 14.8 MeV, which were produced by a neutron generator through a T(d,n)~4He reaction. Induced gamma-ray activities were measured using a low background gamma ray spectrometer equipped with a high resolution HPGe detector. In the cross-section calculations, corrections were made regarding the effects of gamma-ray attenuation, dead-time, fluctuation of the neutron flux, and low energy neutrons. The measured cross-sections were compared with the literature data, evaluation data(ENDF-B/VII.1, JENDL-4.0 and CENDL-3.1), and the results of the model calculation(TALYS1.6).
文摘Objective The relationship between non-high-density lipoprotein(NHDL)cholesterol to high-density lipoprotein cholesterol(HDL-C)ratio(NHHR)and stoke remains unknown.This study aimed to evaluate the association between the adult NHHR and stroke occurrence in the United States of America(USA).Methods To clarify the relationship between the NHHR and stroke risk,this study used a multivariable logistic regression model and a restricted cubic spline(RCS)model to investigate the association between the NHHR and stroke,and data from the National Health and Nutrition Examination Survey(NHANES)from 2005 to 2018.Subgroup and sensitivity analyses were conducted to test the robustness of the results.Results This study included 29,928 adult participants,of which 1,165 participants had a history of stroke.Logistic regression analysis of variables demonstrated a positive association between NHHR and stroke(OR 1.24,95%CI:1.03-1.50,P=0.026).Compared with the lowest reference group of NHHR,participants in the second,third,and fourth quartile had a significantly increased risk of stroke after full adjustments(OR:1.35,95%CI:1.08-1.69)(OR:1.83,95%CI:1.42-2.36)(OR:2.04,95%CI:1.50-2.79).In the total population,a nonlinear dose-response relationship was observed between the NHHR and stroke risk(P non-linearity=0.002).This association remained significant in several subgroup analyses.Further investigation of the NHHR may enhance our understanding of stroke prevention and treatment.Conclusion Our findings suggest a positive correlation between the NHHR and an increased prevalence of stroke,potentially serving as a novel predictive factor for stroke.Timely intervention and management of the NHHR may effectively mitigate stroke occurrence.Prospective studies are required to validate this association and further explore the underlying biological mechanisms.
基金supported by the National Nature Science Foundation of China(No.81072373)the Health Science and Technology Planning Key Project of Hangzhou City(No.HWS2011Z017)
文摘Objective To assess the test-retest reliability and criterion validity of the Simplified Chinese-character version of the International Physical Activity Questionnaire Long form （IPAC）.-L） in urban community-dwelling adults in Hanghzou, China. Methods A total of 158 eligible participants aged 25-59 years from 6 neighbourhoods in two central districts of Hangzhou completed the IPAQ-L questionnaire twice within a 7-day interval. Half of the subjects wore pedometers during the first 7 days. Test-retest reliability was examined by comparing the first （Day 1） and the second （Day 9） survey of IPAQ-L Criterion validity was assessed by comparing IPAO,-L with pedometer data. Results Modest to good test-retest reliability was found with intraclass correlation coefficients of 0.67 for total PA, 0.37 to 0.73 for specific dimensions, and 0.56 to 0.71 for different intensities of PA. Total PA measured by IPAO.-L was moderately correlated with exercise levels （partial r = 0.27, P = 0.020） and walking distance （partial r = 0.31, P = 0.007）, which were measured by a pedometer, after adjusting for gender, age, educational attainment and employment status. Conclusion Our results indicate that the IPAO.-L is a reliable and validated measure for assessing physica activity levels in this population and possibly the adult population in other mainland Chinese cities.
基金financially supported by the Janbazan Medical and Engineering Research Center.
文摘Background:The ankle-foot injuries are among the war-related injuries that cause many serious secondary problems for a lifetime.This nationwide study aimed to assess health-related quality of life and the ability to perform activities of daily living in veterans with ankle-foot injuries due to the Iran-Iraq war.Methods:A total of 1079 veterans with ankle-foot injuries were enrolled in a cross-sectional study from 2014 to 2016.Demographic characteristics,including age,gender,marital status,disability percent,educational level,employment and additional injuries,were collected.The ability to perform daily activities was assessed using the Barthel activities of daily living(ADL)and Lawton instrumental activities of daily living(IADL)Indexes.Physical and mental health-related quality of life(HRQOL)data were measured via the SF-36 subscales.The data were compared with those of bilateral lower limb amputees(BLLAs)and of the general Iranian population.Statistical analyses,including Pearson’s correlation coefficient,one-sample t-test and analysis of variance(ANOVA),were performed using SPSS16.0.A multiple linear regression model was used to determine the contribution of independent variables to the Physical Component Summary(PCS)and Mental Component Summary(MCS).Results:The highest and lowest scores were observed for mental health(48.93±20.69)and bodily pain(28.16±21.74),respectively.The mean scores of veterans with ankle-foot injuries on the SF-36 were significantly lower on all eight measures than those of the general Iranian male population and of the bilateral lower limb amputees(P<0.001).The mean scores of ADLs and IADLs were 83.9±16.3 and 5.3±2.0,respectively.The higher dependency in ADLs(P<0.001)and IADLs(P<0.001),the higher disability rate(P<0.001)and additional injury(P<0.001)were significant determinants of the PCS.ADL(P<0.001)and IADL(P<0.001)limitations,additional injury(P<0.001),history of hospitalization in the year preceding the study(P=0.007)and employment(P=0.001)were reported as determinants of the MCS.Conclusion:The results strongly suggest that veterans with ankle-foot injuries suffer from critically poor healthrelated quality of life.The main predicting factors of HRQOL were the disability to perform ADLs/IADLs,suffering two or more injuries,a history of hospitalization in the year preceding the study and unemployment.
基金financially supported by Guangdong Province Basic and Applied Basic Research Fund Project(Grant No.2022B1515250009)Liaoning Provincial Natural Science Foundation-Doctoral Research Start-up Fund Project(Grant No.2024-BSBA-05)+1 种基金Major Science and Technology Innovation Project in Shandong Province(Grant No.2024CXGC010803)the National Natural Science Foundation of China(Grant Nos.52271269 and 12302147).
文摘The umbilical,a key component in offshore energy extraction,plays a vital role in ensuring the stable operation of the entire production system.The extensive variety of cross-sectional components creates highly complex layout combinations.Furthermore,due to constraints in component quantity and geometry within the cross-sectional layout,filler bodies must be incorporated to maintain cross-section performance.Conventional design approaches based on manual experience suffer from inefficiency,high variability,and difficulties in quantification.This paper presents a multi-level automatic filling optimization design method for umbilical cross-sectional layouts to address these limitations.Initially,the research establishes a multi-objective optimization model that considers compactness,balance,and wear resistance of the cross-section,employing an enhanced genetic algorithm to achieve a near-optimal layout.Subsequently,the study implements an image processing-based vacancy detection technique to accurately identify cross-sectional gaps.To manage the variability and diversity of these vacant regions,the research introduces a multi-level filling method that strategically selects and places filler bodies of varying dimensions,overcoming the constraints of uniform-size fillers.Additionally,the method incorporates a hierarchical strategy that subdivides the complex cross-section into multiple layers,enabling layer-by-layer optimization and filling.This approach reduces manufac-turing equipment requirements while ensuring practical production process feasibility.The methodology is validated through a specific umbilical case study.The results demonstrate improvements in compactness,balance,and wear resistance compared with the initial cross-section,offering novel insights and valuable references for filler design in umbilical cross-sections.
基金supported by an Innovation Fund for Medical Sciences of the Chinese Academy of Medical Sciences (Grant No.2019-I2M-2-007).
文摘Objective:Postpartum nutrition plays a critical role in maternal recovery and long-term health.However,the nutritional status of working mothers in the postpartum period remains understudied.This study aimed to assess the dietary quality of postpartum women in urban Beijing,identify occupational-related factors influencing their diet,and explore potential interventions to improve maternal nutrition during the postpartum period.Methods:In this cross-sectional analysis,554 women one year after delivery were recruited from ten community health centers.Sociodemographic,occupational and postpartum care variables were collected via questionnaire.Dietary intake over the preceding year was assessed using a food frequency questionnaire.The modified dietary balance index for postpartum women were used for dietary quality assessment.Results:The study revealed severe dietary imbalances among postpartum women,characterized by excessive consumption of cereals,eggs,and meats,while their intake of vegetables,fruits,and dairy products was inadequate.According to dietary balance index for postpartum women,66.25%of mothers showed varying degrees of excessive intake.45.31%of mothers experienced varying levels of insufficient intake,with only 19.86%of participants having a relatively balanced diet.Occupational differences were observed,with women in the commercial employment group showing higher levels of excessive food intake.The analysis of influencing factors showed that family monthly income,maternity leave,and postpartum care significantly affected the dietary quality.Conclusions:Postpartum women in Beijing experience widespread dietary imbalances,with both excesses and deficiencies.Occupational context and related factors significantly shape diet quality.These findings highlight the need for targeted nutritional interventions tailored to the specific challenges of different occupational groups.
基金National Nonprofit Institute Research Grant for the Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences:Mechanism of Regulating Phlegm-Dampness Constitution to Prevent Metabolic Diseases based on Gut Microbiota-host DNA Methylation(No.YZ-202151)。
文摘OBJECTIVE:To investigate the difference in gut microbiota between population with damp-heat constitution(DHC)and balanced constitution(BC).METHODS:A multi-centered cross-sectional casecontrol study was conducted,which included 249 participants with damp-heat constitution or balanced constitution.Baseline information of participants was collected,and stool samples were collected for gut microbiota analysis.Principal coordinate analysis,linear discriminant analysis effect size analysis,receiver operating characteristic,random forest model,and phylogenetic investigation of communities by reconstruction of unobserved states methods were used to reveal the relationship between gut microbiota and the damp-heat constitution.RESULTS:Compared to those in the BC group,the richness and diversity of the microbiota,specifically those of several short-chain fatty acid producing genera such as Barnesiella,Coprobacter,and Butyricimonas,were significantly decreased in the DHC group.Regarding biological functions,flavonoid biosynthesis,propanoate metabolism,and nucleotide sugar metabolism were suppressed,while arachidonic acid metabolism and glutathione metabolism were enriched in the DHC group.Finally,a classifier based on the microbiota was constructed to discriminate between the DHC and BC populations.CONCLUSION:The gut microbiota of the DHC population exhibits significantly reduced diversity and is closely related to inflammation,metabolic disorders,and liver steatosis,which is consistent with clinical observations,thus serving as a potential diagnostic tool for traditional Chinese medicine constitution discrimination.
基金supported by the National Natural Science Foundation of China (Grant Nos.52304227 and 52104133)Scientific and Technological Research Platform for Disaster Prevention and Control of Deep Coal Mining (Anhui University of Science and Technology) (Grant No.DPDCM2208).
文摘To investigate the effects of the maximum principal stress direction(θ)and cross-section shape on the failure characteristics of sandstone,true-triaxial compression experiments were conducted using cubic samples with rectangular,circular,and D-shaped holes.Asθincreases from 0°to 60°in the rectangular hole,the left failure location shifts from the left corner to the left sidewall,the left corner,and then the floor,while the right failure location shifts from the right corner to the right sidewall,right roof corner,and then the roof.Furthermore,the initial failure vertical stress first decreases and then increases.In comparison,the failure severity in the rectangular hole decreases for variousθvalues as 30°>45°>60°>0°.With increasingθ,the fractal dimension(D)of rock slices first increases and then decreases.For the rectangular and D-shaped holes,whenθ=0°,30°,and 90°,D for the rectangular hole is less than that of the D-shaped hole.Whenθ=45°and 60°,D for the rectangular hole is greater than that of the D-shaped hole.Theoretical analysis indicates that the stress concentration at the rectangular and D-shaped corners is greater than the other areas.The failure location rotates with the rotation ofθ,and the failure occurs on the side with a high concentration of compressive stress,while the side with the tensile and compressive stresses remains relatively stable.Therefore,the fundamental reason for the rotation of failure location is the rotation of stress concentration,and the external influencing factor is the rotation ofθ.
基金National Key Research and Development Program of China(2023YFB3810200 to J.L.)National Natural Science Foundation of China(92168204,82225030 to J.L.)Fundamental Research Funds for the Central Universities(22120210586 to J.L.)。
文摘Reproductive hormones associated with the hypothalamic-pituitary-gonadal(HPG)axis are closely linked to bone homeostasis.In this study,we demonstrate that Gonadotropin inhibitory hormone(GnIH,one of the key reproductive hormones upstream of the HPG axis)plays an indispensable role in regulating bone homeostasis and maintaining bone mass.We find that deficiency of GnIH or its receptor Gpr147 leads to a significant reduction in bone mineral density(BMD)in mice primarily by enhancement of osteoclast activation in vivo and in vitro.Mechanistically,GnIH/Gpr147 inhibits osteoclastogenesis by the PI3K/AKT,MAPK,NF-κB and Nfatc1 signaling pathways.Furthermore,GnIH treatment was able to alleviate bone loss in aging,ovariectomy(OVX)or LPS-induced mice.Moreover,the therapy using green light promotes the release of GnIH and rescues OVX-induced bone loss.In humans,serum GnIH increases and bone resorption markers decrease after green light exposure.Therefore,our study elucidates that GnIH plays an important role in maintaining bone homeostasis via modulating osteoclast differentiation and demonstrates the potential of GnIH therapy or green light therapy in preventing osteoporosis.
文摘Calcineurin(CN)is a calcium-and calmodulindependent serine/threonine that has been studied in many model organisms including yeast,filamentous fungi,plants,and mammals.Its biological functions range from ion homeostasis and virulence in lower eukaryotes to T-cell activation in humans by human nuclear factors of activated T-cells.CN is a heterodimeric protein consisting of a catalytic subunit,calcineurin A(Cna1p),which contains an active site with a dinuclear metal center,and a regulatory Ca^(2+) binding subunit called calcineurin B(Cnb1p)required to activate Cna1p.The calcineurin B subunit has been highly conserved through evolution:For example,the mammalian calcineurin B shows 54%identity with calcineurin B from Saccharomyces cerevisiae.
文摘The objective of this study was to characterize sympathetic nervous system （SNS） activity in girls with adolescent idiopathic scoliosis （AIS） and investigate the correlation of such activity with body weight and bone mass. Fifty-eight girls with AIS and 21 healthy controls were recruited. Abnormal growth pattern （lower weight, lower body mass index （BMI）, and longer arm span） and significantly increased adrenaline （AD） level, norepinephrine （NE） level, and low-frequency （LF） were found in girls with AIS. Circulating AD level, NE level, and LF power were negatively correlated with body weight and BMI but significantly correlated with bone quality.
