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基于DIA蛋白质组学探讨刺五加提取物治疗转基因帕金森小鼠的作用机制 被引量:2
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作者 郑淇 卢意 +4 位作者 于栋华 赵良友 林春盛 卢芳 刘树民 《中国实验方剂学杂志》 北大核心 2025年第8期40-50,共11页
目的:结合数据非依赖型质谱采集(DIA)蛋白质组学技术,探讨刺五加提取物(ASH)治疗帕金森(PD)小鼠的作用机制。方法:使用α-突触核蛋白(α-Syn)过表达的转基因小鼠构建PD模型,随机分为模型组、刺五加组和美多芭组,另取同月龄雄性C57BL/6... 目的:结合数据非依赖型质谱采集(DIA)蛋白质组学技术,探讨刺五加提取物(ASH)治疗帕金森(PD)小鼠的作用机制。方法:使用α-突触核蛋白(α-Syn)过表达的转基因小鼠构建PD模型,随机分为模型组、刺五加组和美多芭组,另取同月龄雄性C57BL/6小鼠作为空白组,每组各8只。刺五加组给予61.25 mg·kg^(-1)的刺五加提取物,美多芭组给予97.5 mg·kg^(-1)的美多芭溶液,每日1次,灌胃20 d后,采用爬杆时间和自主活动次数对各组小鼠的运动能力进行评估;苏木素-伊红(HE)染色观察PD小鼠黑质区域神经元的变化情况;免疫组化(IHC)检测黑质区域酪氨酸羟化酶(TH)活性及纹状体区域α-Syn面密度;采用DIA定量蛋白质组学技术比较各组小鼠黑质区域的蛋白表达差异;并用IHC验证关键差异蛋白乳铁蛋白(Lactotransferrin)、神经源性位点切口同源物蛋白2(Notch2)、N-Myc下游调节因子2(Ndrg2)、跨膜蛋白166(TMEM 166)表达。用细胞增殖与活性检测试剂盒-8(CCK-8)考察刺五加提取物对α-Syn过表达的PD细胞模型活力的影响,并采用实时荧光定量聚合酶链式反应(Real-time PCR)与蛋白免疫印迹法(Western blot)检测α-Syn过表达的PD细胞中Lactotransferrin、Notch2、Ndrg2、TMEM 166蛋白及mRNA表达。结果:与空白组比较,模型组小鼠爬杆时间显著延长,协调能力变差,自主活动次数降低(P<0.01),神经元肿胀数量减少;与模型组比较,刺五加和美多芭可以显著缩短小鼠的爬杆时间,显著增加自主运动次数(P<0.01),改善神经元的损伤。与空白组比较,模型组小鼠黑质区域TH活性显著下降,纹状体区域α-Syn堆积增多(P<0.01);与模型组比较,刺五加组TH活性明显增强,α-Syn堆积减少(P<0.05)。蛋白质组学分析结果表明,与空白组比较,模型组共筛出464个差异表达蛋白(DEPs),其中323个上调,141个下调;与模型组比较,刺五加组共筛出DEPs 262个,其中85个上调,177个下调。京都基因与基因组百科全书(KEGG)显示刺五加主要调节了Notch信号通路,与空白组比较,模型组Notch2、Ndrg2和TMEM 166的表达上调,Lactotransferrin的表达下调(P<0.01);与模型组比较,刺五加组Notch2、Ndrg2和TMEM 166的表达下调(P<0.05),Lactotransferrin的表达上调(P<0.01),IHC结果与蛋白质组学一致。体外实验结果显示,与空白组比较,模型组细胞Notch2、Ndrg2和TMEM 166 mRNA与蛋白表达水平显著升高(P<0.01),Lactotransferrin mRNA与蛋白表达水平显著降低(P<0.01);与模型组比较,刺五加组细胞Notch2、Ndrg2、TMEM 166 mRNA与蛋白表达水平显著降低(P<0.01),Lactotransferrin mRNA与蛋白表达明显增加(P<0.05,P<0.01)。结论:ASH可能通过下调Notch2、Ndrg2的表达,从而协同阻断Notch信号通路,降低神经元损伤;并通过上调Lactotransferrin,下调TMEM 166的表达,来修复大脑铁积累,干预铁死亡,抑制线粒体自噬,阻止活性氧(ROS)损伤,保护神经细胞达到治疗帕金森的作用。 展开更多
关键词 刺五加提取物 帕金森病 数据非依赖型质谱采集(dia)定量蛋白质组学 α-突触核蛋白(α-Syn)
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Development and Integration of a Data Acquisition System for SST-1 Phase-1 Plasma Diagnostics 被引量:1
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作者 Amit K SRIVASTAVA Manika SHARMA +3 位作者 Imran MANSURI Atish SHARMA Tushar RAVAL Subrata PRADHAN 《Plasma Science and Technology》 SCIE EI CAS CSCD 2012年第11期1002-1007,共6页
Long pulse (of the order of 1000 s or more) SST-1 tokamak experiments demand a data acquisition system that is capable of acquiring data from various diagnostics channels without losing useful data (and hence physi... Long pulse (of the order of 1000 s or more) SST-1 tokamak experiments demand a data acquisition system that is capable of acquiring data from various diagnostics channels without losing useful data (and hence physics information) while avoiding unnecessary generation of a large volume data. SST-1 Phase-1 tokamak operation has been envisaged with data acquisition of several essential diagnostics channels. These channels demand data acquisition at a sampling rate ranging from 1 kilo samples per second (KSPS) to 1 mega samples per second (MSPS). Considering the technical characteristics and requirements of the diagnostics, a data acquisition system based on PXI and CAMAC has been developed for SST-1 plasma diagnostics. Both these data acquisition systems are scalable. Present data acquisition needs involving slow plasma diagnostics are catered by the PXI based data acquisition system. On the other hand, CAMAC data acquisition hardware meets all requirements of the SST-1 Phase-1 fast plasma diagnostics channels. A graphical user interface for both data acquisition systems (PXI and CAMAC) has been developed using LabVIEW application development software. The collected data on the local hard disk are directly streaming to the central server through a dedicated network for post-shot data analysis. This paper describes the development and integration of the data acquisition system for SST-1 Phase-1 plasma diagnostics. The integrated testing of the developed data acquisition system has been performed using SST-1 central control and diagnostics signal conditioning units. In the absence of plasma shots, the integrated testing of the data acquisition system for the initial diagnostics of SST-1 Phase-1 operation has been performed with simulated physical signals. The primary engineering objective of this integrated testing is to validate the performance of the developed data acquisition system under simulated conditions close to that of actual tokamak operation. The data acquisition is synchronized with a clock and trigger provided by the central timing system. 展开更多
关键词 SST-1 data acquisition CAMAC PXI plasma diagnostics
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基于4D-DIA蛋白质组学分析黄芩素通过抑制核糖体生物功能治疗哮喘 被引量:1
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作者 鲁瑶 韦丽妮 +10 位作者 王瑞琪 杨婧雯 张凯琪 颜岚 刘洋 施长琪 张璐璐 赵宁 何小鹃 吕诚 李立 《中国中药杂志》 北大核心 2025年第19期5512-5520,共9页
评价黄芩素对哮喘小鼠的药效作用,并通过蛋白质组学分析黄芩素抑制核糖体生物功能的作用机制。将Balb/c小鼠随机分为正常组,模型组,孟鲁司特组,黄芩素低、中、高剂量组,建立OVA致敏小鼠哮喘模型评价黄芩素的治疗作用。通过肺功能测试、... 评价黄芩素对哮喘小鼠的药效作用,并通过蛋白质组学分析黄芩素抑制核糖体生物功能的作用机制。将Balb/c小鼠随机分为正常组,模型组,孟鲁司特组,黄芩素低、中、高剂量组,建立OVA致敏小鼠哮喘模型评价黄芩素的治疗作用。通过肺功能测试、苏木素-伊红(HE)染色、刚果红染色、肺指数、支气管肺泡灌洗液炎性细胞计数评价肺组织病理学改变和肺部损伤情况,脾指数评价免疫功能受损情况,酶联免疫吸附测定(ELISA)法评价肺部炎症因子的严重程度,四维数据独立采集(4D-DIA)蛋白质组学方法分析黄芩素治疗哮喘的差异蛋白,生物信息学分析核糖体相关的生物功能,分子对接技术和免疫组化技术验证核糖体相关蛋白,即大核糖体亚基蛋白L23(RPL23A)、小核糖体亚基蛋白eS25(RPS25)和DNA导向的RNA聚合酶Ⅰ和Ⅲ亚基RPAC1(POLR1C)的表达情况。结果显示,与模型组比较,黄芩素高剂量组小鼠的气道高反应性、肺指数和脾指数显著降低,肺组织损伤减轻,白细胞介素(IL)-4、IL-5和IL-13的表达显著降低。4D-DIA蛋白质组学及生物信息学分析,提示黄芩素主要通过抑制核糖体生物功能发挥作用。免疫组化结果显示,与模型组相比,黄芩素高剂量组小鼠肺组织中RPL23A、RPS25、POLR1C蛋白表达水平明显下降,结果与蛋白质组学分析的结果一致。黄芩素能够显著改善哮喘小鼠的症状,其机制可能与抑制RPL23A、RPS25、POLR1C等核糖体生物功能有关。 展开更多
关键词 黄芩素 哮喘 4D-dia蛋白质组学 核糖体
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1例2p16.1区域缺失致Dias-Logan综合征的遗传学分析
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作者 唐燕青 赖允丽 +4 位作者 覃再隆 易赏 陈秋莉 易坚 罗静思 《中国优生与遗传杂志》 2025年第3期642-646,共5页
目的探讨1例2p16.1区域缺失导致Dias-Logan综合征患儿的临床表型和遗传学特征,为临床诊治提供参考依据。方法选取2020年10月于广西壮族自治区妇幼保健院就诊的生长发育迟缓、智力低下的1例6岁患儿为研究对象,使用全外显子组测序(WES)和... 目的探讨1例2p16.1区域缺失导致Dias-Logan综合征患儿的临床表型和遗传学特征,为临床诊治提供参考依据。方法选取2020年10月于广西壮族自治区妇幼保健院就诊的生长发育迟缓、智力低下的1例6岁患儿为研究对象,使用全外显子组测序(WES)和低深度高通量基因组测序(low-pass GS)对患儿进行遗传变异分析,可疑变异应用PCR-琼脂糖凝胶电泳和Sanger测序进行验证。同时验证其父母以明确变异来源。结果患儿临床表现为智力障碍、语言和运动发育迟缓、步态不稳、特殊面容、小阴茎、尿道下裂、隐睾,头颅磁共振成像(MRI)示局灶性脱髓鞘病变。WES提示BCL11A基因第1~第4号外显子杂合缺失,low-pass GS显示染色体2p16.1区域存在250 kb的杂合性缺失,PCR-琼脂糖凝胶电泳和Sanger测序进一步证实患儿在该区域存在缺失。缺失区域包含单倍剂量不足的BCL11A基因,诊断为Dias-Logan综合征,属于致病性突变。其父母的low-pass GS和PCR-琼脂糖凝胶电泳均未见异常,提示该缺失为新发变异。结论根据患儿的临床表型和遗传学分析,上述患儿考虑为仅有BCL11A基因缺失导致的Dias-Logan综合征,为该疾病的临床诊断和遗传咨询提供依据。 展开更多
关键词 全外显子组测序 低深度高通量基因组测序 2p16.1区域缺失 BCL11A基因 dias-Logan综合征
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Application of Rough Set Theory in Fault Diagnostic Rules Acquisition 被引量:3
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作者 殷晨波 周庆敏 李永生 《Journal of Donghua University(English Edition)》 EI CAS 2007年第2期276-279,共4页
Rough set theory is a new mathematical tool to deal with vagneness and uncertainty. But original rough sets theory only generates deterministic rules and deals with data sets in which there is no noise. The variable p... Rough set theory is a new mathematical tool to deal with vagneness and uncertainty. But original rough sets theory only generates deterministic rules and deals with data sets in which there is no noise. The variable precision rough set model (VPRSM) is presented to handle uncertain and noisy information. A method based on VPRSM is proposed to apply to fault diagnosis feature extraction and rules acquisition for industrial applications. An example for fault diagnosis of rotary machinery is given to show that the method is very effective. 展开更多
关键词 variable precision rough set fault diagnosisrules acquisition
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Endoscopic ultrasound assessment and tissue acquisition of mediastinal and abdominal lymph nodes 被引量:3
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作者 Giacomo Tamanini Anna Cominardi +2 位作者 Nicole Brighi Pietro Fusaroli Andrea Lisotti 《World Journal of Gastrointestinal Oncology》 SCIE 2021年第10期1475-1491,共17页
The differential diagnosis between benign and malignant lymph nodes(LNs)has a fundamental role in the characterization and staging of malignant conditions,as well as in subsequent patients’management.All imaging moda... The differential diagnosis between benign and malignant lymph nodes(LNs)has a fundamental role in the characterization and staging of malignant conditions,as well as in subsequent patients’management.All imaging modalities(i.e.computed tomography and magnetic resonance imaging)rely mainly on size;endoscopic ultrasound(EUS)criteria based on B-mode evaluation and Doppler features fail to adequately characterize with high specificity LNs nature.The introduction of EUS-elastography and contrast-enhanced harmonic EUS are useful techniques to increase the diagnostic yield in identifying metastatic LNs,to identify which suspicious LN should require pathological characterization and,finally,to target tissue acquisition.EUS-guided tissue acquisition(EUS-TA)is increasingly being used for diagnosing lymphadenopathy whenever the characterization modifies patients’subsequent management and when no superficial LN is accessible.Since target therapy are currently available(i.e.lung cancer,breast cancer),EUS-TA of malignant LNs could be required to identify tumor biology.In this field,both fine needle aspiration and biopsy needles are able to guarantee accurate results with almost perfect specificity and sub-optimal sensitivity.We finally propose a diagnostic algorithm based on most recent,high-level evidence for the diagnostic approach to suspected LNs assessment. 展开更多
关键词 Endoscopic ultrasound-guided tissue acquisition Endoscopic ultrasound-fine needle aspiration Endoscopic ultrasound-fine needle biopsy BIOPSY ASPIRATION Cancer
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基于DIA技术的多药耐药性捻转血矛线虫蛋白质组学分析
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作者 李炎夫 贾晓晴 +10 位作者 马梅清 杜山 焦万明 马园 郝陆瑶 张艳妮 李倩楠 郭志凯 丁玉林 陆静 王瑞 《中国兽医杂志》 北大核心 2025年第5期65-72,共8页
为探索驱虫药耐药性产生的机制,本试验对捻转血矛线虫(Haemonchus contortus)的第3期幼虫(L3)以伊维菌素、阿苯达唑和左旋咪唑药物进行处理,采用数据非依赖性采集(DIA)蛋白质组测序技术对捻转血矛线虫L3经药物作用前后的蛋白质进行差异... 为探索驱虫药耐药性产生的机制,本试验对捻转血矛线虫(Haemonchus contortus)的第3期幼虫(L3)以伊维菌素、阿苯达唑和左旋咪唑药物进行处理,采用数据非依赖性采集(DIA)蛋白质组测序技术对捻转血矛线虫L3经药物作用前后的蛋白质进行差异表达分析,以筛选表达差异的多药耐药性相关蛋白质。结果显示,共鉴定出1 888个差异表达蛋白质,其中63个与多药耐药性相关;进一步的深入分析揭示,有36个蛋白质的表达差异显著(P <0.05),而25个是捻转血矛线虫特有的、与耐药性相关的差异表达蛋白质。基因本体论(GO)分析结果显示,共有60个差异表达耐药性蛋白质被归类于生物学领域的76个分支中;京都基因与基因组百科全书(KEGG)分析结果显示,有46个表达水平不同的蛋白质富集到脂质代谢和内分泌系统等75条信号通路中。综合分析发现,有5个捻转血矛线虫特有的耐药性蛋白质表达水平差异显著,分别为含有UDP-葡萄糖醛基转移酶结构域的部分蛋白质、抗原H11、免疫球蛋白v组和i组、含有酪氨酸蛋白激酶(PTK)结构域的蛋白质及含有致命巨幼虫同源结构域2的蛋白质,这些蛋白质均参与重要的生物学过程和关键代谢通路。本试验所获结果为揭示差异表达蛋白质与捻转血矛线虫脂类代谢、内分泌系统和多药耐药性的关系提供了基础数据,对探明捻转血矛线虫多药耐药性产生的机制具有重要意义。 展开更多
关键词 多药耐药性 捻转血矛线虫 数据非依赖性采集(dia) 蛋白质组学 耐药性相关蛋白质
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基于DIA定量蛋白质组学解析电针对阿尔茨海默病模型大鼠认知功能的改善机制
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作者 毕钰桢 罗粤铭 +4 位作者 赵海梅 程晶 郭红 胡毅澜 程红 《中国医药指南》 2025年第33期60-64,共5页
目的采用数据非依赖采集(DIA)定量技术对接受电针干预的阿尔茨海默病(AD)模型大鼠海马组织进行蛋白质组学分析,探究电针改善AD模型大鼠认知功能的作用机制。方法本研究于2023年10月至2023年11月在深圳市众循精准医学研究院进行,将15只A... 目的采用数据非依赖采集(DIA)定量技术对接受电针干预的阿尔茨海默病(AD)模型大鼠海马组织进行蛋白质组学分析,探究电针改善AD模型大鼠认知功能的作用机制。方法本研究于2023年10月至2023年11月在深圳市众循精准医学研究院进行,将15只AD模型大鼠用随机数字表法分为电针治疗组(ZD组)、假针刺组(ZW组)及模型组(M组),每组5只。ZD组于双侧“天枢”“曲池”及“足三里”穴位行电针治疗;ZW组于上述穴位旁开5 mm非经穴处实施假电针干预;M组仅接受与ZD组等时长的束缚固定,不施加任何针刺或电刺激。各组每日干预1次,每次30 min,连续14 d,干预结束后进行新物体识别测试(NORT)和DIA定量蛋白质组学分析。结果NORT显示,ZD组辨别指数(DI)高于ZW组和M组,差异有统计学意义(P<0.05),其总探索时间长于ZW组和M组,差异有统计学意义(P<0.05)。DIA定量蛋白质组学分析表明,相较于M组,ZD组海马组织中鉴定出16个差异上调表达蛋白及13个差异下调表达蛋白。差异表达蛋白主要定位于细胞质膜,GO富集分析显示其在突触及突触后结构富集,KEGG富集分析揭示其在谷氨酸能突触富集,Reactome富集分析则表明其富集在信号转导通路。结论电针可有效改善AD模型大鼠的认知功能障碍,其作用机制可能通过调控谷氨酸能突触相关的差异表达蛋白,进而改善突触功能,促进兴奋性神经信号传导。 展开更多
关键词 阿尔茨海默病 电针 大鼠 认知功能 数据非依赖采集 蛋白质组学
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An Intermediate Frequency Acquisition Scheme for S-band Single Access Link of the Tracking and Data Relay Satellite System 被引量:1
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作者 马雨出 Gu +2 位作者 Xuemai Zhang Naitong 《High Technology Letters》 EI CAS 2001年第1期31-36,共6页
A nonzero intermediate frequency (IF) likelihood acquisition scheme designed for S-band Single Access (SSA) link of China’s Tracking and Data Relay Satellite System (CTDRSS) is introduced. The received signal is down... A nonzero intermediate frequency (IF) likelihood acquisition scheme designed for S-band Single Access (SSA) link of China’s Tracking and Data Relay Satellite System (CTDRSS) is introduced. The received signal is downconverted to IF, and then direct sampled in IF using a 1-bit A/D. After the digitalization, the sampled data is detected using a hybrid likelihood acquisition scheme. Using this structure, large noise figure of the analog mixer or active filters, amplitude and phase imbalance between low-frequency in-phase and quandrature-phase channel can be avoided. An easy designing algorithm of the acquisition scheme is also derived. The performance and algorithm are verified by computer simulation. 展开更多
关键词 Digital receiver Likelihood detector PN code acquisition Satellite communications
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基于4D-FastDIA定量蛋白质组的富硒与非富硒鸡蛋差异表达蛋白鉴定及功能分析
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作者 袁琳 赵文竹 +2 位作者 吴春剑 于一丁 于志鹏 《食品科学》 北大核心 2025年第12期163-170,共8页
本研究旨在利用四维快速数据非依赖性采集(4D-fast data-independent acquisition,4D-FastDIA)定量蛋白质组学技术,解析富硒鸡蛋与非富硒鸡蛋中差异表达的蛋白及其功能特性。通过液相色谱-串联质谱分析技术结合生物信息学方法,比较组间... 本研究旨在利用四维快速数据非依赖性采集(4D-fast data-independent acquisition,4D-FastDIA)定量蛋白质组学技术,解析富硒鸡蛋与非富硒鸡蛋中差异表达的蛋白及其功能特性。通过液相色谱-串联质谱分析技术结合生物信息学方法,比较组间相对定量的蛋白质,探讨蛋白质的差异表达规律,并利用基因本体论注释分析和京都基因与基因组百科全书通路富集分析法对蛋白质功能和代谢通路进行分析。蛋白质组学分析共鉴定到257种蛋白质,其中251种蛋白质被定量分析。蛋清中共鉴定到48种差异蛋白,其中28种蛋白表达下调,20种蛋白表达上调。蛋黄中共鉴定到82种差异蛋白,其中58种蛋白表达下调,24种蛋白表达上调。通过比较天然富硒鸡蛋与非富硒鸡蛋中蛋白质的表达水平,发现丝氨酸肽酶抑制剂、免疫球蛋白样结构域蛋白、卵转铁蛋白和黏蛋白-6在富硒鸡蛋中的表达水平显著高于非富硒鸡蛋。通过基因本体注释分析和京都基因与基因组百科全书通路富集分析发现,天然富硒鸡蛋中与免疫调节、抗菌、抗氧化相关的蛋白质在表达水平上显著高于非富硒鸡蛋。研究结果可为揭示富硒鸡蛋与非富硒鸡蛋在营养成分价值上的差异及其分子机制提供科学依据。 展开更多
关键词 鸡蛋 天然富硒 非富硒 蛋白质组学 四维快速数据非依赖性采集
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Research on Genetic Algorithm Based Knowledge Auto Acquisition for Fault Diagnosis
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作者 张雪江 朱向阳 +1 位作者 钟秉林 黄仁 《Journal of Southeast University(English Edition)》 EI CAS 1996年第2期32-37,共6页
In this paper, a genetic algorithm based knowledge auto acquisition approach for fault diagnosis is proposed. Under the circumstances that diagnostic examples are available but no empirical knowledge can be obtained,... In this paper, a genetic algorithm based knowledge auto acquisition approach for fault diagnosis is proposed. Under the circumstances that diagnostic examples are available but no empirical knowledge can be obtained, knowledge for fault diagnosis can be 展开更多
关键词 GENETIC algorithm KNOWLEDGE AUTO acquisition FAULT diagnosis
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Data Processing and Acquisition System for the HT-7 Multipulse Thomson Scattering Diagnostic
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作者 陈卓天 赵君煜 +2 位作者 方自深 李亚东 杨利 《Plasma Science and Technology》 SCIE EI CAS CSCD 2003年第4期1867-1872,共6页
This article describes the data processing and acquisition system for the HT-7 mul-tipulse Thomson scattering diagnostic. An eight-pulse laser is used in the Thomson scattering system to obtain electron temperature pr... This article describes the data processing and acquisition system for the HT-7 mul-tipulse Thomson scattering diagnostic. An eight-pulse laser is used in the Thomson scattering system to obtain electron temperature profiles at eight different times throughout an entire plasma discharge. The major components of the diagnostic system consist of a multipulse Nd-glass laser, a photodetector's subsystem, a calibration set and a CAMAC data processing and acquisition system. The data processing software along with LeCroy 2250L will perform the data acquisition. In order to simplify the operation and extend the capability of its compatibility with other math softwares, the processing software has been improved by the authors. The new software based on the VC++ easily utilizes some math softwares to calculate the electron temperature. The new software is simpler and more operational than the old one. 展开更多
关键词 laser Thomson scattering diagnostic electron temperature data acquisition
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A New Electronic Instrumentation Approach for the Acquisition of Microscopic Blood Smear Images for the Automatic Diagnosis of Anemia
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作者 Jerome Nango Alico Sié Ouattara Alain Clement 《Advances in Bioscience and Biotechnology》 2020年第6期237-255,共19页
Anemia is a blood abnormality that affects the quantity and quality of red blood cells in the human body. This sometimes banal sign spares no continent and no social stratum. This anomaly is generally appreciated thro... Anemia is a blood abnormality that affects the quantity and quality of red blood cells in the human body. This sometimes banal sign spares no continent and no social stratum. This anomaly is generally appreciated through biological analyzes of patients</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> blood. These analyzes, which boil down to the knowledge of hemato-metric constants, cannot by themselves allow the characterization of certain forms of anemia in the sense that most anemia are related to the morphology and color of red blood cells. Our work in this paper is to perform blood smears on patients and perform a morphological and colorimetric analysis of red blood cells on these smears. This approach allowed us to highlight on each erythrocyte morphological and colorimetric descriptors to accurately identify the types of anemia by image processing methods. This identification is performed in an automated environment to allow pathologists to respond quickly to anemia-related emergencies and also improve the treatment to be conducted. This automation required the implementation of a new approach to electronic instrumentation and the acquisition of microscopic blood smear images for the automatic and rapid diagnosis of anemia. 展开更多
关键词 ANEMIA INSTRUMENTATION acquisition Automated Blood Smears Red Blood Cells Morphological Descriptors Colorimetric Descriptors Image Processing diagnosis
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Development of Multilevel Meteorological Data Acquisition System for Radiation Emergency Decision Support
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作者 D.N. Krishnakumar A. Bagavath Singh Anjukumari R. Baskaran M.T. Jose B. Venkatraman 《Journal of Earth Science and Engineering》 2013年第8期545-553,共9页
This paper describes a development of multilevel meteorological data acquisition system implemented at Kalpakkam coastal site for atmospheric dispersion and model validation studies. Meteorological data are one of the... This paper describes a development of multilevel meteorological data acquisition system implemented at Kalpakkam coastal site for atmospheric dispersion and model validation studies. Meteorological data are one of the most important inputs into any air dispersion model. As a part of atmospheric dispersion modeling studies and developing a methodology to forecast the site-specific dispersion characteristics, the real time monitoring of meteorological parameters assumes significance. This is achieved by erecting met towers instrumented at multilevel and single level at different locations with sensors for measuring various meteorological parameters. Real-world data logging applications involve not only just acquiring and recording signals, but also combination of offline analysis, display, report generation and data sharing. This paper covers development of low cost compact MMDAS (modular meteorological data acquisition system), its performance evaluation, field deployment test and data comparison analysis with fast response and high accuracy internationally acclaimed sonic anemometer. The system is based on embedded modules from Advantech and is designed to acquire analogue and digital signals from a multilevel instrumented met tower. The collected data are transferred from remote base station to central server for storage and further processing using wireless interface. MMDAS has many advantages like cost effectiveness, less complex signal conditioning electronics and easy maintenance. This system has good application during radiation emergency as well as site specific meteorological data collection and model validation studies. 展开更多
关键词 Radiation emergency meteorological data acquisition MMDAS dispersion modeling sonic anemometer.
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Probes Into Application of Multi-media Technology in Teaching Chinese as the Second Language Acquisition Class
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作者 Ling-wei YIN 《Sino-US English Teaching》 2017年第3期154-156,共3页
The multi-media teaching method refers to using computer as a teaching assistant in the teaching Chinese as the second language (TCSL) class. The purpose of employing multi-media technology is to encourage students... The multi-media teaching method refers to using computer as a teaching assistant in the teaching Chinese as the second language (TCSL) class. The purpose of employing multi-media technology is to encourage students' class participation, giving them explicit and specific impression of abstract contents and cultivating their intuitive grasp of linguistic points. Based on the current study of usage of multi-media technology in class teaching, this paper attempts to investigate mainly on its positive influence of multi-media technology in teaching. 展开更多
关键词 multi-media technology second language acquisition active class participation
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Negative Transfer of Guanzhong Plain's Dialect on the Acquisition of English Sound
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作者 王莉 《海外英语》 2018年第13期224-226,共3页
Pronunciation learning is the key factor in second language acquisition,which is also the soul of the language.Thereforeit is very vital for beginners to learn the right sounds.However,In the second language acquisiti... Pronunciation learning is the key factor in second language acquisition,which is also the soul of the language.Thereforeit is very vital for beginners to learn the right sounds.However,In the second language acquisition,the sounds of learners’first lan-guage unavoidably have some positive and negative transfer on the target language acquisition.To the Chinese learners,as Chineselanguage belongs to Sino-Tibetan system while English belongs to Germanic language system,the negative transfer is much moreobvious.Guanzhong plain lies in the central of Shaanxi province of China.The dialect here belongs to one of the sub-branches ofNorthern Mandrarin Dialect.Which shares little similarities with English in phonetics.The paper bases on the research of 25 stu-dents whose first language are Guanzhong Plain’s dialect to compare the phonemes of two languages in general and to further ana-lyze the negative transfer of Guanzhong Plain’s dialect to English Phonetic and give the related solutions. 展开更多
关键词 NEGATIVE transfer Guanzhong Plain' sdialect acquisition of ENGLISH SOUNDS
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Role of Gender Towards Income Generation: An Impact Analysis of Land Acquisition in West Bengal, India
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作者 Arup Majumder 《Sociology Study》 2016年第10期639-652,共14页
One of the principal fundamentals of post-independence development projects in India has been land acquisition. Most of these projects have caused major changes in land use, leading to dispossession and displacement o... One of the principal fundamentals of post-independence development projects in India has been land acquisition. Most of these projects have caused major changes in land use, leading to dispossession and displacement of a large number of people and their involuntary resettlement in other areas. Development induced displacement brings loss of productive assets, dismantling of social networks, destruction of ancestral property, and even increased morbidity and mortality rates in some instances. Therefore, this paper seeks to explore further the micro-level impact of agricultural land acquisition on livelihood pattern of both genders to the establishment of the industry on the fertile agricultural land in the k'haragpur subdivision of Paschim Medinipur district in the early 1990s with the cooperation of West Bengal Government, India. Land acquisition without any policy of rehabilitation caused not only landlessness but also created joblessness among the affected population and at the family level, it has resulted food insecurity and increased workload among the women. The women on the other hand made desperate attempts to cope up the burden by working within and outside the domestic domain. 展开更多
关键词 Land acquisition GENDER development induced displacement INDUSTRY agricultural land
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Phosphorus acquisition,translocation,and redistribution in maize 被引量:2
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作者 Hui-Ling Guo Meng-Zhi Tian +1 位作者 Xian Ri Yi-Fang Chen 《Journal of Genetics and Genomics》 2025年第3期287-296,共10页
Phosphorus(P)is an essential nutrient for crop growth,making it important for maintaining food security as the global population continues to increase.Plants acquire P primarily via the uptake of inorganic phosphate(P... Phosphorus(P)is an essential nutrient for crop growth,making it important for maintaining food security as the global population continues to increase.Plants acquire P primarily via the uptake of inorganic phosphate(Pi)in soil through their roots.Pi,which is usually sequestered in soils,is not easily absorbed by plants and represses plant growth.Plants have developed a series of mechanisms to cope with P deficiency.Moreover,P fertilizer applications are critical for maximizing crop yield.Maize is a major cereal crop cultivated worldwide.Increasing its P-use efficiency is important for optimizing maize production.Over the past two decades,considerable progresses have been achieved in studies aimed at adapting maize varieties to changes in environmental P supply.Here,we present an overview of the morphological,physiological,and molecular mechanisms involved in P acquisition,translocation,and redistribution in maize and combine the advances in Arabidopsis and rice,to better elucidate the progress of P nutrition.Additionally,we summarize the correlation between P and abiotic stress responses.Clarifying the mechanisms relevant to improving P absorption and use in maize can guide future research on sustainable agriculture. 展开更多
关键词 Plant nutrient Phosphorus acquisition Phosphorus translocation Phosphorus-use efficiency Corn yield
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