Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immu...Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immunoglobulin levels in JIA patients are significantly higher than their control during the active state of disease. Methodology: This prospective observational study was conducted over a period of 18 months All the newly diagnosed oligo-articular and poly-articular JIA patients having active disease were included by purposive sampling. Data were collected by a semi-structured predesigned questionnaire. Result: Most of the study subjects (57.6%) belonged to age group > 3 - 9 years. Oligo JIA was diagnosed in 66.7% and poly JIA in 33.3% of JIA children. The difference in mean (±SD) ESR (33.52 ± 21.29 and 15.09 ± 7.71 mm in 1st hour) at active and inactive states was highly significant. Mean (±SD) difference of IgG, IgM and IgA in active and inactive states of disease were highly significant. Conclusion: Higher and abnormal levels of immunoglobulin (IgG, IgM, and IgA) were present among JIA patients in active disease state which became normal during inactive disease state after treatment.展开更多
Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged ent...Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.展开更多
Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upo...Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upon their structural and biological properties.To some extent,the modification of natural products is quite different from de novo structure-based drug discovery.This review describes the general strategies and principles for the modification of natural products to drugs,as illustrated by several successful medicines that originated from natural products.展开更多
Accurate atmospheric correction(AC)is vital for ocean color remote sensing,especially in turbid coastal waters where the traditional black pixel assumption in the near-infrared(NIR)bands fails due to substantial water...Accurate atmospheric correction(AC)is vital for ocean color remote sensing,especially in turbid coastal waters where the traditional black pixel assumption in the near-infrared(NIR)bands fails due to substantial water-leaving radiance.This study evaluates the influence of temperature-dependent variability in the absorption coefficient of pure seawater(aw)on the widely used NIR iterative atmospheric correction algorithm(ACiter).A modified algorithm,ACiter-T,is proposed by incorporating temperature-adjusted aw based on empirical measurements.Simulated datasets,covering a wide range of water temperatures,suspended particulate matter(SPM),and chlorophyll-a concentrations,were used alongside over 500 satellite-in situ matchups from AERONET-OC sites.Results demonstrate that in turbid waters,especially when the sea surface temperature deviates from the reference(22℃)by more than 10℃,the use of temperature-sensitive aw markedly improves retrieval accuracy.The modified ACiter-T algorithm notably reduced remote sensing reflectance(Rrs)bias and mean absolute percentage difference(MAPD)in the blue spectral bands(e.g.,410 nm),with MAPD reductions exceeding 50% in highly turbid and cold water conditions.In contrast,for less turbid waters or when the deviation of water temperature is small,temperature corrections exert minimal influence.These findings highlight the necessity of incorporating temperature-dependent optical parameters into AC frameworks to ensure robust ocean color product accuracy under variable environmental conditions.展开更多
Rice-derived peptides are a sustainable protein source with emerging lipid regulation functions.In this study,peptides with novel pancreatic lipase(PL)and cholesterol esterase(CE)inhibition from rice protein hydrolysa...Rice-derived peptides are a sustainable protein source with emerging lipid regulation functions.In this study,peptides with novel pancreatic lipase(PL)and cholesterol esterase(CE)inhibition from rice protein hydrolysates(RPHs)were identified by peptidomics.Then their alleviating effects and mechanisms on lipid accumulation in cells were investigated.55 peptides were identified,showing significant enrichment of Phe,Pro,Arg,and Leu,with hydrophobic/polar amino acids at terminal positions.Among them,five peptides(WQ7,FR6,GF6,GA7,and GY7)exhibited potent inhibitory activity against PL and CE,with IC_(50)values of 0.19-0.70μg/mL and 0.22-0.25μg/mL,respectively.Molecular docking studies revealed that five peptides bind stably to the active sites of PL(Phe77,Tyr114,Phe215,and His263)and CE(Ser194 and His435)through hydrogen bonding,PIalkyl,and PI-PI interactions.Dynamic simulations demonstrated that three peptides(WQ7,FR6,and GF6)form stable complexes with both CE and PL,exhibiting strong binding affinity.In vitro study confirmed that these peptides reduced levels of TC,TG,and LDL-c while increasing HDL-c content,with WQ7 showing superior efficacy.Western blot and qPCR results indicated WQ7 regulated lipid metabolism by suppressing the PPARγpathway and downregulating the protein expression of related factors,such as ACS,FASN,and SREBP-1c.This study showed that the WQ7,a multifunctional bioactive peptide,demonstrates direct enzyme-inhibitory activity and regulates core transcriptional pathways,providing theoretical support for utilizing rice protein in the development of lipid-lowering functional food.展开更多
文摘Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immunoglobulin levels in JIA patients are significantly higher than their control during the active state of disease. Methodology: This prospective observational study was conducted over a period of 18 months All the newly diagnosed oligo-articular and poly-articular JIA patients having active disease were included by purposive sampling. Data were collected by a semi-structured predesigned questionnaire. Result: Most of the study subjects (57.6%) belonged to age group > 3 - 9 years. Oligo JIA was diagnosed in 66.7% and poly JIA in 33.3% of JIA children. The difference in mean (±SD) ESR (33.52 ± 21.29 and 15.09 ± 7.71 mm in 1st hour) at active and inactive states was highly significant. Mean (±SD) difference of IgG, IgM and IgA in active and inactive states of disease were highly significant. Conclusion: Higher and abnormal levels of immunoglobulin (IgG, IgM, and IgA) were present among JIA patients in active disease state which became normal during inactive disease state after treatment.
基金Thisprojectissupportedbythenaturalsciencefundofthenation (No 39840 0 11)andthatofJiangsuprovince (BK970 5 7)
文摘Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.
文摘Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upon their structural and biological properties.To some extent,the modification of natural products is quite different from de novo structure-based drug discovery.This review describes the general strategies and principles for the modification of natural products to drugs,as illustrated by several successful medicines that originated from natural products.
基金supported by the National Natural Science Foundation of China(grant numbers T2222010 and 42306197)the Civilian Aerospace Technology Pre-research Program(grant number D010202)the National Key Research and Development Program of China(grant numbers 2022YFC3104900 and 2022YFC3104903).
文摘Accurate atmospheric correction(AC)is vital for ocean color remote sensing,especially in turbid coastal waters where the traditional black pixel assumption in the near-infrared(NIR)bands fails due to substantial water-leaving radiance.This study evaluates the influence of temperature-dependent variability in the absorption coefficient of pure seawater(aw)on the widely used NIR iterative atmospheric correction algorithm(ACiter).A modified algorithm,ACiter-T,is proposed by incorporating temperature-adjusted aw based on empirical measurements.Simulated datasets,covering a wide range of water temperatures,suspended particulate matter(SPM),and chlorophyll-a concentrations,were used alongside over 500 satellite-in situ matchups from AERONET-OC sites.Results demonstrate that in turbid waters,especially when the sea surface temperature deviates from the reference(22℃)by more than 10℃,the use of temperature-sensitive aw markedly improves retrieval accuracy.The modified ACiter-T algorithm notably reduced remote sensing reflectance(Rrs)bias and mean absolute percentage difference(MAPD)in the blue spectral bands(e.g.,410 nm),with MAPD reductions exceeding 50% in highly turbid and cold water conditions.In contrast,for less turbid waters or when the deviation of water temperature is small,temperature corrections exert minimal influence.These findings highlight the necessity of incorporating temperature-dependent optical parameters into AC frameworks to ensure robust ocean color product accuracy under variable environmental conditions.
基金supported by the National Natural Science Foundation of China(32572484)Natural Science Foundation of Hunan Province(2022JJ30587,2022JJ50218)Science and Technology Innovation Program of Hunan Province(2024JK2145).
文摘Rice-derived peptides are a sustainable protein source with emerging lipid regulation functions.In this study,peptides with novel pancreatic lipase(PL)and cholesterol esterase(CE)inhibition from rice protein hydrolysates(RPHs)were identified by peptidomics.Then their alleviating effects and mechanisms on lipid accumulation in cells were investigated.55 peptides were identified,showing significant enrichment of Phe,Pro,Arg,and Leu,with hydrophobic/polar amino acids at terminal positions.Among them,five peptides(WQ7,FR6,GF6,GA7,and GY7)exhibited potent inhibitory activity against PL and CE,with IC_(50)values of 0.19-0.70μg/mL and 0.22-0.25μg/mL,respectively.Molecular docking studies revealed that five peptides bind stably to the active sites of PL(Phe77,Tyr114,Phe215,and His263)and CE(Ser194 and His435)through hydrogen bonding,PIalkyl,and PI-PI interactions.Dynamic simulations demonstrated that three peptides(WQ7,FR6,and GF6)form stable complexes with both CE and PL,exhibiting strong binding affinity.In vitro study confirmed that these peptides reduced levels of TC,TG,and LDL-c while increasing HDL-c content,with WQ7 showing superior efficacy.Western blot and qPCR results indicated WQ7 regulated lipid metabolism by suppressing the PPARγpathway and downregulating the protein expression of related factors,such as ACS,FASN,and SREBP-1c.This study showed that the WQ7,a multifunctional bioactive peptide,demonstrates direct enzyme-inhibitory activity and regulates core transcriptional pathways,providing theoretical support for utilizing rice protein in the development of lipid-lowering functional food.
