Auto-catalysts were the largest consumers of platinum group metals and the most important secondary resources, recovery of PGMs from spent auto-catalysts by leaching with various acidities were investigated. The leach...Auto-catalysts were the largest consumers of platinum group metals and the most important secondary resources, recovery of PGMs from spent auto-catalysts by leaching with various acidities were investigated. The leaching thermodynamics of PGMs at 363 K was first discussed. At 363 K the higher the acidities of HCl, the higher the leaching recoveries of PGMs, and the sequence of leaching recovery was Pd>Pt>Rh. When H2SO4 used alone, the leaching recoveries of PGMs was low, when the acidity of H2SO4 increasing, the leaching recovery of Rh kept stable.展开更多
Ketones are one of the most common functional groups, and ketone-containing compounds are essential in both the nature and the chemical sciences. As such, the acidities (pKa) of ketones provide valuable information fo...Ketones are one of the most common functional groups, and ketone-containing compounds are essential in both the nature and the chemical sciences. As such, the acidities (pKa) of ketones provide valuable information for scientists to screen for biological activities, to determine physical properties or to study reaction mechanisms. Direct measurements of pKa of ketones are not readily available due to their extremely weak acidity. Hence, a quantitative structure-property relationship (QSPR) model that can predict the acidities of ketones and their acidity order is highly desirable. The establishment of an acidity scale in dimethyl sulfoxide (DMSO) solution by Bordwell et al. made such an effort possible. By utilizing the pKa values of forty-eight ketones determined in DMSO as the training set, a QSPR model for predicting acidities of ketones was built by stepwise multiple linear regression analysis. The established model showed statistical significance and predictive power (r2 = 0.91, q2 = 0.86, s = 1.42). Moreover, the QSPR model also gave reasonable acidity predictions for five ketones in an external prediction set that were not included in the model generation phase (r2 = 0.92, s = 1.618). Overall, the reported QSPR model for predicting acidities of ketones provides a useful tool for both biologists and chemists in understanding the biophysical properties and reaction rates of different classes of ketones.展开更多
Important effects exist between precious metals and rare earths oxides in three-way catalyst, especially the coordinated effects. These effects were studied by using H2PtCl6, Pt(NH3)2(NO2)2 and Pt(OH)2(C2H5ONH2)2 as P...Important effects exist between precious metals and rare earths oxides in three-way catalyst, especially the coordinated effects. These effects were studied by using H2PtCl6, Pt(NH3)2(NO2)2 and Pt(OH)2(C2H5ONH2)2 as Pt precursors, and the mixed oxide of (Ce-Zr-La-Pr)O as base material to prepare a series of catalysts, and their performances of the catalysts were studied by TPR and CO pulse titration technologies. The results shown that Pt precursors and their solutions pH values influenced the oxygen storage capabilities, the active metal distribution degrees of the catalysts obviously, and every catalyst prepared by different precursors had an optimal pH values. It indicates that the active metals precursors and their solutions acidities have outstanding influences on the catalysts performances for the mutual effects existing between the active metals and the Rare Earth metal oxides, which results from the mate groups of the precursors and the solution acidity.展开更多
Activation of neutrophil membrane receptors initiates intracellular signal transduction cascades that orchestrate the cell's effector functions,including phagocytosis,production of reactive oxygen and halogen spec...Activation of neutrophil membrane receptors initiates intracellular signal transduction cascades that orchestrate the cell's effector functions,including phagocytosis,production of reactive oxygen and halogen species,degranulation,and NETosis(formation of neutrophil extracellular traps[NETs]).NETs,which contain antimicrobial compounds such as myeloperoxidase(MPO),represent a strategy to combat infection.However,excessive production of NETs promotes thrombosis,diabetes mellitus,and other diseases.Therefore,investigations into the mechanisms of NETosis and the identification of modulators of this process are critical for developing strategies to address NETosis-related disorders.Here,we identified a novel NETosis inducer,human serum albumin(HSA)modified by the MPO product hypochlorous acid(HSAHOCl),whose accumulation in vivo was correlated with inflammatory processes.Using human blood neutrophils,we investigated HSAHOCl-induced NETosis and detected NET formation by flow cytometry.The results showed that the mechanism of HSAHOClinduced NETosis involved MPO,NADPH oxidase,and phosphatidylinositol 3-kinases(PI3Ks),and that HSAHOCl activated a reactive oxygen species-dependent suicidal type of NETosis.Moreover,HSAHOCl-induced NETosis was inhibited by an anti-HSAHOCl monoclonal antibody.Thus,our findings may facilitate the development of strategies to modulate NETosis in inflammation associated with elevated MPO activity.展开更多
We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework...We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework incorporates(1)grid configuration,(2)transport dynamics,(3)chemical mechanisms,(4)aerosol processes,(5)wet/dry deposition parameterizations,and(6)heterogeneous chemistry treatments associated with sulfate,nitrous acid(HONO)chemistry,and aerosol/cloud–photolysis interactions(APIs/CPIs).Openly shared with the atmospheric research community,the model facilitates integration of advanced physicochemical schemes to enhance simulation accuracy.Globally,the model demonstrates realistic representations of ozone(O_(3))and aerosol optical depth.The EPICC model generally demonstrates robust performance in simulating regional concentrations of O_(3) and PM_(2.5)(and its components)in China.It successfully captures vertical profiles of both global and regional O_(3).Notably,the model mitigates frequently reported sulfate underestimations in highly industrialized regions of China.The model accurately captures two regional severe pollution episodes observed in eastern China(January/June 2021).Sensitivity experiments highlight the critical roles of heterogeneous chemical mechanisms associated with sulfate,HONO chemistry,APIs,and CPIs in capturing PM_(2.5) and O_(3) concentrations in China.Improved sulfate mechanisms result in an increase of approximately 32.4%(2.8μg m^(−3))in simulated winter sulfate concentrations when observations exceed 10μg m^(−3).Enhanced HONO elevates winter O_(3) and PM_(2.5) by≤20 and≤10μg m^(−3),respectively.Overall,CPIs dominate over APIs in improving O_(3) and PM_(2.5) simulations across China.Locally,APIs mitigate PM_(2.5) and O_(3) discrepancies in the Sichuan Basin.Seasonal cloud–chemistry coupling explains the weaker impact of PM_(2.5) in summer.展开更多
Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicin...Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicinal properties.Galloylation and glycosylation are key steps in the formation of polyphenolic compounds,as the glycosylation of flavonoids is required for the acylation of flavonoid glycosides,and the glycosylation of gallic acid is necessary for its role as an acyl donor.Therefore,glycosylation to generate the acyl donor or acceptor is a core step in the biosynthesis of polyphenolic compounds.However,how this glycosylation occurs in P.chinense is unknown.In this study,we determined that the UDP-glucose transferase PcUGT84A82 mediates the glycosylation of gallic acid and pinocembrin to produce 1-O-Galloyl-β-D-glucose and pinocembroside,respectively.Metabolic profiling of polyphenolic compounds using UHPLC-ESI–Q-TOF/MS revealed high levels of polyphenols in flowers,leaves,and roots,and low levels in stems of P.chinense.We performed isoform-sequencing(Iso-seq)to assemble a full-length transcriptome of P.chinense,from which we identified 58 UGT family members.PcUGT84A82 is highly similar to functional UGTs in other plant species,and PcUGT84A82 transcript levels were positively correlated with the levels of various polyphenolic compounds.We validated the function of PcUGT84A82 via in vitro enzyme assays and transient expression in Nicotiana benthamiana leaves.Subcellular localization tests showed that PcUGT84A82 localizes to the nucleus and cytoplasm.In summary,PcUGT84A82 catalyzes the conversion of gallic acid to 1-O-Galloyl-β-D-glucose as the acyl donor and pinocembrin to pinocembroside as the acyl acceptor,mediating the biosynthesis of galloylated macrocyclic polyphenolic compounds in P.chinense.These findings lay the foundation for elucidating the entire biosynthetic pathway of active polyphenols in this important herbal plant species.展开更多
Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’...Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’s disease.In this review,we summarize the changes in short-chain fatty acid levels and the abundance of short-chain fatty acid-producing bacteria in various samples from patients with Parkinson’s disease,highlighting the critical role of gut homeostasis imbalance in the pathogenesis and progression of the disease.Focusing on the nervous system,we discuss the molecular mechanisms by which short-chain fatty acids influence the homeostasis of both the enteric nervous system and the central nervous system.We identify key processes,including the activation of G protein-coupled receptors and the inhibition of histone deacetylases by short-chain fatty acids.Importantly,structural or functional disruptions in the enteric nervous system mediated by these fatty acids may lead to abnormalα-synuclein expression and gastrointestinal dysmotility,which could serve as an initiating event in Parkinson’s disease.Furthermore,we propose that short-chain fatty acids help establish communication between the enteric nervous system and the central nervous system via the vagal nerve,immune circulation,and endocrine signaling.This communication may shed light on their potential role in the transmission ofα-synuclein from the gut to the brain.Finally,we elucidate novel treatment strategies for Parkinson’s disease that target short-chain fatty acids and examine the challenges associated with translating short-chain fatty acid-based therapies into clinical practice.In conclusion,this review emphasizes the pivotal role of short-chain fatty acids in regulating gut-brain axis integrity and their significance in the pathogenesis of Parkinson’s disease from the perspective of the nervous system.Moreover,it highlights the potential value of short-chain fatty acids in early intervention for Parkinson’s disease.Future research into the molecular mechanisms of short-chain fatty acids and their synergistic interactions with other gut metabolites is likely to advance the clinical translation of innovative short-chain fatty acid-based therapies for Parkinson’s disease.展开更多
Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(...Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(4,4'-dihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid),phen(1,10-phenanthroline),bpb(1,4-bis(pyrid-4-yl)benzene),bpa(bis(4-pyridyl)amine),and copper,nickel and cadmium chlorides at 160℃.The products were isolated as stable crystalline solids and were characterized by IR spectra,elemental analyses,thermogravimetric analyses,and singlecrystal X-ray diffraction analyses.Single-crystal X-ray diffraction analyses revealed that three complexes crystallize in the monoclinic P21/n,tetragonal I42d,and orthorhombic P21212 space groups.The complexes exhibit molecular dimers(1)or 2D metal-organic networks(2 and 3).The catalytic performances in the Knoevenagel reaction of these complexes were investigated.Complex 1 exhibits an effective catalytic activity and excellent reusability as a heterogeneous catalyst in the Knoevenagel reaction at room temperature.CCDC:2463800,1;2463801,2;2463802,3.展开更多
Lignin-derived oxygenated aromatics,particularly phenols and aromatic ethers obtained through depolymerization,represent promising feedstocks for synthesizing high-density and high-heat-sink aviation fuels via alkylat...Lignin-derived oxygenated aromatics,particularly phenols and aromatic ethers obtained through depolymerization,represent promising feedstocks for synthesizing high-density and high-heat-sink aviation fuels via alkylation-hydrogenation processes.This study systematically evaluates the catalytic performance of various zeolites(Hβ,HZSM-5,MCM-41 and HUSY)in the alkylation reaction of phenol with cyclohexanol.Characterization results demonstrate that HUSY zeolite showed superior catalytic activity compared to other zeolites,attributable to its favorable pore architecture and well-balanced acid site distribution that synergistically facilitate molecular diffusion and catalytic transformations.To further enhance the catalytic properties,HUSY zeolite was modified with citric acid at various concentrations and compared with those treated with NaOH and oxalic acid.The results revealed that citric acid treatment preserved the crystallinity of the zeolite while modulating its acid distribution and pore structure.All modified zeolites enhanced phenol alkylation activity.Notably,the HUSY-0.5M catalyst,which exhibited the highest medium-strong acid to total acid ratio,achieved superior catalytic performance,80.4%conversion of phenol and 99.6%selectivity for alkylation products.The catalyst also exhibited high activity in the alkylation of various lignin-derived compounds,demonstrating its broad applicability.This work provides a new strategy for the valorization of lignin-derived phenols into high-value fuel precursors through alkylation.展开更多
Three zinc(Ⅱ),nickel(Ⅱ),and cadmium(Ⅱ)complexes,namely[Zn(μ-Htpta)(py)_(2)]n(1),[Ni(H_(2)biim)2(H_(2)O)2][Ni(tpta)(H_(2)biim)2(H_(2)O)]2·3H_(2)O(2),and[Cd_(3)(μ4-tpta)2(μ-dpe)_(3)]_(n)(3),have been construc...Three zinc(Ⅱ),nickel(Ⅱ),and cadmium(Ⅱ)complexes,namely[Zn(μ-Htpta)(py)_(2)]n(1),[Ni(H_(2)biim)2(H_(2)O)2][Ni(tpta)(H_(2)biim)2(H_(2)O)]2·3H_(2)O(2),and[Cd_(3)(μ4-tpta)2(μ-dpe)_(3)]_(n)(3),have been constructed hydrothermally at 160℃ using H_(3)tpta([1,1':3',1″-terphenyl]-4,4',5'-tricarboxylic acid),py(pyridine),H_(2)biim(2,2'-biimidazole),dpe(1,2-di(4-pyridyl)ethylene),and zinc,nickel and cadmium chlorides,resulting in the formation of stable crystalline solids which were subsequently analyzed using infrared spectroscopy,element analysis,thermogravimetric analysis,as well as structural analyses conducted via single-crystal X-ray diffraction.The findings from these single-crystal Xray diffraction studies indicate that complexes 1-3 form crystals within the monoclinic system P2_(1)/c space group(1)or triclinic system P1 space group(2 and 3),and possess 1D,0D,and 3D structures,respectively.Complex 1 demonstrated substantial catalytic efficiency and excellent reusability as a heterogeneous catalyst in the reaction of Knoevenagel condensation under ambient temperature conditions.In addition,complex 1 also showcased notable anti-wear performance when used in polyalphaolefin synthetic lubricants.CCDC:2449810,1;2449811,2;2449812,3.展开更多
Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted ...Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted acid site(BAS)strength on reaction pathways,alongside the spatial proximity effects between BAS and Mo active sites in bifunctional synergy,remains a critical scientific challenge in catalyst design.This study systematically tunes both BAS strength(via isomorphous metal substitution)and Mo-BAS spatial proximity in zeolites,integrating MDA catalytic evaluations with density functional theory(DFT)calculations to dissect their individual contributions.Strongly acidic BAS catalysts(compared to moderately acidic Fe/Ga-substituted counterparts)exhibit superior performance,evidenced by enhanced aromatic yields.Conversely,weakly acidic Bsubstituted zeolites demonstrate optimal mono-/bifunctional synergy,outperforming moderate-acid systems.DFT results reveal that acid strength dictates C−H activation mechanisms by modulating the energy barriers of rate-determining steps.While Al-zeolites deliver the highest activity,B-substituted systems display unique potential for mechanistic investigations.Spatial proximity analysis indicates that micrometer-scale Mo-BAS distances hinder effective synergy due to exceeding electron interaction and mass transfer limits,whereas nanometer-scale proximity enhances activity(via accelerated intermediate transport)and suppresses coke formation.These findings establish a theoretical framework for rationalizing zeolite catalyst optimization through BAS property engineering and spatial control of Mo-BAS cooperation,providing actionable guidelines for designing next-generation MDA catalysts.展开更多
This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Compa...This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Comparative analysis of natural air(Air),controlled atmosphere(CA),and MAP treatments revealed that MAP upregulated the expression of the hercynylcysteine S-oxide synthase(HCSOS),aldehyde dehydrogenase(ALDH),and monoamine oxidase(MAO)genes,thereby enhancing histidine-derived ergothioneine and methionine levels,and subsequently boosting glutathione-mediated redox homeostasis.Meanwhile,MAP induced the expression of the dihydroxyacid dehydratase(DHAD),saccharopine dehydrogenase(SDH),and arginosuccinate lyase(ASL)genes,redirecting valine,lysine,and arginine into the tricarboxylic acid(TCA)cycle to fuel ATP production.MAP also enhanced ASL-mediated arginine degradation and urea cycle activity,reducing arginine accumulation when compared to CA treatment.In contrast,while MAP induced upregulated expression of the pyrroline-5-carboxylate dehydrogenase(P5CDH)and D-amino acid oxidase(DAAO)genes,CA treatment promoted proline accumulation,reflecting stress-specific metabolic flexibility.Collectively,these findings demonstrate that MAP triggers transcriptional reprogramming of amino acid metabolism to coordinate oxidative defense,energy generation,and osmotic balance.By modulating these metabolic pathways and regulatory genes under MAP conditions,fungal adaptability can be disrupted.Hence,this study provides a promising strategy for suppressing green mold development,extending the postharvest shelf life,and improving the quality of fruits and vegetables.展开更多
Non-naturalα-amino acids(α-AAs)are pivotal in drug and catalysis,yet their synthesis remains challenges.A photocatalytic strategy for the direct construction of N-heteroaryl-containingα-AAs from heteroaryl aldehyde...Non-naturalα-amino acids(α-AAs)are pivotal in drug and catalysis,yet their synthesis remains challenges.A photocatalytic strategy for the direct construction of N-heteroaryl-containingα-AAs from heteroaryl aldehydes via acyl radical intermediates is reported.This method exhibits exceptional functional group tolerance and enables late-stage diversification of peptides and carbonyl-group derivatization.Mechanistic studies confirm a radical-based pathway,while applications in peptide modification highlight its utility in bioorganic chemistry.展开更多
The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-ri...The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-rich components of CGFS(CGFS-H)and systematically investigates the selective leaching behavior of Fe^(3+),Al^(3+)and Ca^(2+)using three organic acid extractants,i.e.,citric acid,tartaric acid,and tetrasodium iminodisuccinate.Additionally,the stepwise leaching of iron,aluminum and calcium from CGFS-H is explored.The selective dissolution mechanisms of these metals by different organic acids are elucidated through X-ray diffraction(XRD),X-ray fluorescence(XRF),and scanning electron microscopy(SEM)analyses.The results indicate that tetrasodium iminodisuccinate exhibits the highest leaching selectivity for Fe^(3+),while tartaric acid demonstrateds a comparable affinity for both Fe^(3+)and Al^(3+).In contrast citric acid shows superior selectivity toward Ca^(2+).The leaching yield of Fe^(3+),Al^(3+)and Ca^(2+)after sequential leaching with the three organic acids were 79.8%,65.08%and 78.6%,respectively.These findings confirm that effective and selective separation of Fe^(3+),Al^(3+)and Ca^(2+)from CGFS-H can be achieved via optimized organic acid-based leaching strategies.This advancement provides a critical foundation for developing Ca/Fe/Al hydrotalcite materials using CGFS-H as a sustainable feedstock,thereby facilitating the transformation of waste residue into high-value functional materials and promoting resourceefficient utilization of coal gasification fine slag.展开更多
Under hydrothermal and solvothermal conditions,two novel cobalt-based complexes,{[Co_(2)(CIA)(OH)(1,4-dtb)]·3.2H_(2)O}n(HU23)and{[Co_(2)(CIA)(OH)(1,4-dib)]·3.5H2O·DMF}n(HU24),were successfully construct...Under hydrothermal and solvothermal conditions,two novel cobalt-based complexes,{[Co_(2)(CIA)(OH)(1,4-dtb)]·3.2H_(2)O}n(HU23)and{[Co_(2)(CIA)(OH)(1,4-dib)]·3.5H2O·DMF}n(HU24),were successfully constructed by coordinatively assembling the semi-rigid multidentate ligand 5-(1-carboxyethoxy)isophthalic acid(H₃CIA)with the Nheterocyclic ligands 1,4-di(4H-1,2,4-triazol-4-yl)benzene(1,4-dtb)and 1,4-di(1H-imidazol-1-yl)benzene(1,4-dib),respectively,around Co^(2+)ions.Single-crystal X-ray diffraction analysis revealed that in both complexes HU23 and HU24,the CIA^(3-)anions adopt aκ^(7)-coordination mode,bridging six Co^(2+)ions via their five carboxylate oxygen atoms and one ether oxygen atom.This linkage forms tetranuclear[Co4(μ3-OH)2]^(6+)units.These Co-oxo cluster units were interconnected by CIA^(3-)anions to assemble into 2D kgd-type structures featuring a 3,6-connected topology.The 2D layers were further connected by 1,4-dtb and 1,4-dib,resulting in 3D pillar-layered frameworks for HU23 and HU24.Notably,despite the similar configurations of 1,4-dtb and 1,4-dib,differences in their coordination spatial orientations lead to topological divergence in the 3D frameworks of HU23 and HU24.Topological analysis indicates that the frameworks of HU23 and HU24 can be simplified into a 3,10-connected net(point symbol:(4^(10).6^(3).8^(2))(4^(3))_(2))and a 3,8-connected tfz-d net(point symbol:(4^(3))_(2)((4^(6).6^(18).8^(4)))),respectively.This structural differentiation confirms the precise regulatory role of ligands on the topology of metal-organic frameworks.Moreover,the ultraviolet-visible absorption spectra confirmed that HU23 and HU24 have strong absorption capabilities for ultraviolet and visible light.According to the Kubelka-Munk method,their bandwidths were 2.15 and 2.08 eV,respectively,which are consistent with those of typical semiconductor materials.Variable-temperature magnetic susceptibility measurements(2-300 K)revealed significant antiferromagnetic coupling in both complexes,with their effective magnetic moments decreasing markedly as the temperature lowered.CCDC:2457554,HU23;2457553,HU24.展开更多
5,5'-dithiobis(2-nitrobenzoic acid)(H_(2)DTNB)was employed as the second ligand to react with cucurbit[6]uril(Q[6])and Cd(NO_(3))_(2),and it was deprotonated or transformed into HDTNB^(-),TNB^(2-)and NSB^(2-)(H_(2...5,5'-dithiobis(2-nitrobenzoic acid)(H_(2)DTNB)was employed as the second ligand to react with cucurbit[6]uril(Q[6])and Cd(NO_(3))_(2),and it was deprotonated or transformed into HDTNB^(-),TNB^(2-)and NSB^(2-)(H_(2)TNB=5,5'-thiobis(2-nitrobenzoic acid),H_(2)NSB=2-nitro-5-sulfobenzoic acid)under different conditions to afford three novel supramolecular assemblies with the formulas of[Cd(H_(2)O)_(4)(Q[6])](HDTNB)_(2)·3H_(2)O(1),[Cd(H_(2)O)_(6)]_(2)(TNB)_(2)·Q[6]·4H_(2)O(2)and[Cd(H_(2)O)_(5)(NSB)]_(2)·Q[6](3).Singe-crystal diffraction(SC-XRD)analysis revealed that assembly 1 is constructed from 2D[Cd(H_(2)O)_(4)(Q[6])]2+supramolecular layers and HDTNB^(-)supra molecular layers,the structure of assembly 2 is comprised of the 2D{[Cd(H_(2)O)_(6)]_(2)·Q[6]}^(4+)supramolecular layers and 1D TNB^(2-)supramolecular chains,while assembly 3 is built from the 3D Q[6]frameworks with[Cd(H_(2)O)_(5)(NSB)]supramolecular chains filled in the pores.Meanwhile,the noncovalent interactions between the ligands HDTNB^(-)/TNB^(2-)/NSB^(2-)and the outer-surface of Q[6]molecules contributed greatly to the formation of the supramolecular architecture of assemblies 1-3.CCDC:2522253,1;2522254,2;2522255,3.展开更多
The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.Th...The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.The complex was characterized by IR,^(1)H(^(13)C)NMR,elemental analysis,and powder X-ray diffraction,and the crystal structure was determined by single-crystal X-ray diffraction.The crystal belongs to the orthorhombic system with space group Iba2,and the central tin atom is in a five-coordinated trigonal bipyramidal configuration.Quantum chemistry ab initio calculations were performed to investigate the stability,molecular orbital energy,and frontier molecular orbital characteristics of the complex.Additionally,its thermal stability,electrochemical properties,and in vitro anticancer activity were evaluated.CCDC:2380308.展开更多
In this study,polyacrylic acid(PAA)films were employed as a model system,and a series of PAA films with tunable water wettability was systematically prepared by varying molecular weight and curing temperature.Using at...In this study,polyacrylic acid(PAA)films were employed as a model system,and a series of PAA films with tunable water wettability was systematically prepared by varying molecular weight and curing temperature.Using attenuated total reflectance Fourier-transform infrared spectroscopy(ATR-FTIR),the molecular configurations of surface carboxyl groups(COOH),free carboxyl(COOH_(f))and hydrogen-bonded carboxyl(COOH_(HB),were directly correlated with the polar component of surface energy(γ^(s,p)).By decomposing theγ^(s,p)values of the PAA thin films as a sum of the contributions of COOH_(f)and COOH_(H B),the intrinsic polar component of surface energy of COOH_(H B)(γ_(H B)^(s,p*))was quantified for the first time as 8.34 mN/m,significantly lower than that of COOH_(f)(γ_(f)^(s,p*)=34 mN/m).This result highlights that hydrogen bonding markedly reduces theγ^(s,p),providing a rational explanation for the relatively large water contact angle observed on PAA thin films.Furthermore,it establishes a thermodynamic basis for estimating the fraction of surface COOH_(H B)groups(f H B)from wettability measurements.Further extension of the model to carboxyl-terminated self-assembled monolayers(COOH-SAMs)revealed that surface COOH density(ΣCOOH)critically regulates wetting behavior:whenΣCOOH ranges from 4.30 to 5.25 nm^(-2),COOH groups predominantly exist in a free state and facilitate effective hydration layers,thereby promoting superhydrophilicity.Overall,this study not only establishes a unified thermodynamic framework linking surface COOH configurations to macroscopic wettability,but also validates its universality by extending it to COOH-SAMs systems,thereby providing a unified theoretical framework for the controllable design of hydrophilicity in various COOH-functionalized surfaces.展开更多
BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probi...BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probiotics have shown good potential in regulating the endocrine system and improving the intestinal microecology,providing new ideas for the treatment of breast hyperplasia.OBJECTIVE:To explore the effects and mechanisms of traditional Chinese medicine compounds and fermented probiotic compounds on breast hyperplasia in mice,providing new theoretical and experimental bases for the clinical treatment and prevention of breast hyperplasia.METHODS:(1)Network pharmacology tools were used to predict the anti-breast-hyperplasia activity of Herba Gueldenstaedtiae(Euphorbia humifusa),as well as its potential targets and signaling pathways.The databases included:TCMSP,OMIM,GeneCards database,UniProt website,Venny2.1.0 website,Metascape,HERB website,and STRING database,all of which are open-access databases.Network pharmacology can predict and screen key information such as the targets corresponding to the active ingredients of traditional Chinese medicine,disease targets,and action pathways through network analysis and computer-system analysis.Therefore,it has been increasingly widely used in the research of traditional Chinese medicine.(2)A breast hyperplasia model was induced in mice by injecting estrogen and progesterone.Mice in the normal blank group were injected intraperitoneally with normal saline every day.Mice in the model group and drugadministration groups were injected intraperitoneally with estradiol benzoate injection at a concentration of 0.5 mg/kg every day for 25 days.From the 26th day,the injection of estradiol benzoate injection was stopped.Mice in the normal blank group were injected intramuscularly with normal saline every day,and mice in the model group and drug-administration groups were injected intramuscularly with progesterone injection at a concentration of 5 mg/kg for 5 days.After the model was established,each group was given drugs respectively.The normal blank group and the model group were gavaged with 0.2 mL/d of normal saline;the positive blank group(Xiaozheng Pill group)was gavaged with an aqueous solution of Xiaozheng Pill at 0.9 mg/g;the low-,medium-and high-dose groups of Compound Herba Gueldenstaedtiae were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively;the low-,medium-and high-dose groups of traditional Chinese medicine-bacteria fermentation were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively.The administration was continuous for 30 days.RESULTS AND CONCLUSION:(1)The results of network pharmacology research showed that the Compound Herba Gueldenstaedtiae(Euphorbia humifusa)contained 46 active ingredients,which were related to 1213 potential targets.After comparison with 588 known breast-hyperplasia targets,it was speculated that 50 of these targets might be related to the direct effect of the compound on breast hyperplasia.(2)After drug intervention,there was no significant change in the high-dose group of Compound Herba Gueldenstaedtiae compared with the normal blank group.The liver indicators of the other intervention groups all significantly decreased(P<0.05).(3)In terms of kidney and uterine indicators,the medium-dose group of Compound Herba Gueldenstaedtiae decreased significantly compared with the normal blank group(P<0.05).In terms of the uterine index,the model group increased significantly compared with the normal blank group(P<0.01).(4)After 1-month drug treatment,the number of lobules and acini in the breast tissue of the Xiaozheng Pill group,the low,medium,and high-dose group of Compound Herba Gueldenstaedtiae,the low,medium,and highdose groups of traditional Chinese medicine-bacteria fermentation decreased,and the duct openings narrowed.With the increase of drug dose,diffuse hyperplasia of breast tissue was significantly improved.(5)The ELISA results showed that compared with the model group,the estrogen level was lower in the medium-dose group of traditional Chinese medicine-bacteria fermentation after the intervention(P<0.05).In addition,the follicle-stimulating hormone level in the low-dose group of Compound Herba Gueldenstaedtiae was lower than that of the model group(P<0.05).(6)The intervention in the mouse model led to changes in the abundance of short chain fatty acids and intestinal flora in all groups.To conclude,the Compound Herba Gueldenstaedtiae and its probiotic fermentation products significantly improved mammary gland hyperplasia in mice by regulating hormone levels,improving the structure of the gut microbiota,and increasing the content of shortchain fatty acids,providing new ideas and potential sources of drugs for the treatment of breast hyperplasia.展开更多
文摘Auto-catalysts were the largest consumers of platinum group metals and the most important secondary resources, recovery of PGMs from spent auto-catalysts by leaching with various acidities were investigated. The leaching thermodynamics of PGMs at 363 K was first discussed. At 363 K the higher the acidities of HCl, the higher the leaching recoveries of PGMs, and the sequence of leaching recovery was Pd>Pt>Rh. When H2SO4 used alone, the leaching recoveries of PGMs was low, when the acidity of H2SO4 increasing, the leaching recovery of Rh kept stable.
文摘Ketones are one of the most common functional groups, and ketone-containing compounds are essential in both the nature and the chemical sciences. As such, the acidities (pKa) of ketones provide valuable information for scientists to screen for biological activities, to determine physical properties or to study reaction mechanisms. Direct measurements of pKa of ketones are not readily available due to their extremely weak acidity. Hence, a quantitative structure-property relationship (QSPR) model that can predict the acidities of ketones and their acidity order is highly desirable. The establishment of an acidity scale in dimethyl sulfoxide (DMSO) solution by Bordwell et al. made such an effort possible. By utilizing the pKa values of forty-eight ketones determined in DMSO as the training set, a QSPR model for predicting acidities of ketones was built by stepwise multiple linear regression analysis. The established model showed statistical significance and predictive power (r2 = 0.91, q2 = 0.86, s = 1.42). Moreover, the QSPR model also gave reasonable acidity predictions for five ketones in an external prediction set that were not included in the model generation phase (r2 = 0.92, s = 1.618). Overall, the reported QSPR model for predicting acidities of ketones provides a useful tool for both biologists and chemists in understanding the biophysical properties and reaction rates of different classes of ketones.
基金Project supported by the National High Technology Research and Development Programs (863 ) of China (2002 AA321060, 2004AA649040) Yunnan Province Science Technology Program (2004B0028Q)
文摘Important effects exist between precious metals and rare earths oxides in three-way catalyst, especially the coordinated effects. These effects were studied by using H2PtCl6, Pt(NH3)2(NO2)2 and Pt(OH)2(C2H5ONH2)2 as Pt precursors, and the mixed oxide of (Ce-Zr-La-Pr)O as base material to prepare a series of catalysts, and their performances of the catalysts were studied by TPR and CO pulse titration technologies. The results shown that Pt precursors and their solutions pH values influenced the oxygen storage capabilities, the active metal distribution degrees of the catalysts obviously, and every catalyst prepared by different precursors had an optimal pH values. It indicates that the active metals precursors and their solutions acidities have outstanding influences on the catalysts performances for the mutual effects existing between the active metals and the Rare Earth metal oxides, which results from the mate groups of the precursors and the solution acidity.
文摘Activation of neutrophil membrane receptors initiates intracellular signal transduction cascades that orchestrate the cell's effector functions,including phagocytosis,production of reactive oxygen and halogen species,degranulation,and NETosis(formation of neutrophil extracellular traps[NETs]).NETs,which contain antimicrobial compounds such as myeloperoxidase(MPO),represent a strategy to combat infection.However,excessive production of NETs promotes thrombosis,diabetes mellitus,and other diseases.Therefore,investigations into the mechanisms of NETosis and the identification of modulators of this process are critical for developing strategies to address NETosis-related disorders.Here,we identified a novel NETosis inducer,human serum albumin(HSA)modified by the MPO product hypochlorous acid(HSAHOCl),whose accumulation in vivo was correlated with inflammatory processes.Using human blood neutrophils,we investigated HSAHOCl-induced NETosis and detected NET formation by flow cytometry.The results showed that the mechanism of HSAHOClinduced NETosis involved MPO,NADPH oxidase,and phosphatidylinositol 3-kinases(PI3Ks),and that HSAHOCl activated a reactive oxygen species-dependent suicidal type of NETosis.Moreover,HSAHOCl-induced NETosis was inhibited by an anti-HSAHOCl monoclonal antibody.Thus,our findings may facilitate the development of strategies to modulate NETosis in inflammation associated with elevated MPO activity.
基金National Key Scientific and Technological Infrastructure project “Earth System Science Numerical Simulator Facility” (EarthLab)supported by the National Natural Science Foundation of China (Grant No. 92044302)the National Key Research Development Program of China (Grant No. 2022YFC3700703)
文摘We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework incorporates(1)grid configuration,(2)transport dynamics,(3)chemical mechanisms,(4)aerosol processes,(5)wet/dry deposition parameterizations,and(6)heterogeneous chemistry treatments associated with sulfate,nitrous acid(HONO)chemistry,and aerosol/cloud–photolysis interactions(APIs/CPIs).Openly shared with the atmospheric research community,the model facilitates integration of advanced physicochemical schemes to enhance simulation accuracy.Globally,the model demonstrates realistic representations of ozone(O_(3))and aerosol optical depth.The EPICC model generally demonstrates robust performance in simulating regional concentrations of O_(3) and PM_(2.5)(and its components)in China.It successfully captures vertical profiles of both global and regional O_(3).Notably,the model mitigates frequently reported sulfate underestimations in highly industrialized regions of China.The model accurately captures two regional severe pollution episodes observed in eastern China(January/June 2021).Sensitivity experiments highlight the critical roles of heterogeneous chemical mechanisms associated with sulfate,HONO chemistry,APIs,and CPIs in capturing PM_(2.5) and O_(3) concentrations in China.Improved sulfate mechanisms result in an increase of approximately 32.4%(2.8μg m^(−3))in simulated winter sulfate concentrations when observations exceed 10μg m^(−3).Enhanced HONO elevates winter O_(3) and PM_(2.5) by≤20 and≤10μg m^(−3),respectively.Overall,CPIs dominate over APIs in improving O_(3) and PM_(2.5) simulations across China.Locally,APIs mitigate PM_(2.5) and O_(3) discrepancies in the Sichuan Basin.Seasonal cloud–chemistry coupling explains the weaker impact of PM_(2.5) in summer.
基金the National Natural Science Foundation of China(82304659)a Chenguang Project of Shanghai(23CGA52)+2 种基金the Shanghai Municipal Science and Technology Commission 2025 Key Technology R&D Program“Synthetic Biology”Project(25HC2810300)the Key Project at Central Government Level:the Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(2060302)the Science and Technology Development Program of Shanghai University of Traditional Chinese Medicine(23KFL045,23KFL051).
文摘Penthorum chinense Pursh has been used for centuries as an herbal medicine and food in East Asia.The main active substances in P.chinense are galloylated macrocyclic polyphenolic compounds,which have excellent medicinal properties.Galloylation and glycosylation are key steps in the formation of polyphenolic compounds,as the glycosylation of flavonoids is required for the acylation of flavonoid glycosides,and the glycosylation of gallic acid is necessary for its role as an acyl donor.Therefore,glycosylation to generate the acyl donor or acceptor is a core step in the biosynthesis of polyphenolic compounds.However,how this glycosylation occurs in P.chinense is unknown.In this study,we determined that the UDP-glucose transferase PcUGT84A82 mediates the glycosylation of gallic acid and pinocembrin to produce 1-O-Galloyl-β-D-glucose and pinocembroside,respectively.Metabolic profiling of polyphenolic compounds using UHPLC-ESI–Q-TOF/MS revealed high levels of polyphenols in flowers,leaves,and roots,and low levels in stems of P.chinense.We performed isoform-sequencing(Iso-seq)to assemble a full-length transcriptome of P.chinense,from which we identified 58 UGT family members.PcUGT84A82 is highly similar to functional UGTs in other plant species,and PcUGT84A82 transcript levels were positively correlated with the levels of various polyphenolic compounds.We validated the function of PcUGT84A82 via in vitro enzyme assays and transient expression in Nicotiana benthamiana leaves.Subcellular localization tests showed that PcUGT84A82 localizes to the nucleus and cytoplasm.In summary,PcUGT84A82 catalyzes the conversion of gallic acid to 1-O-Galloyl-β-D-glucose as the acyl donor and pinocembrin to pinocembroside as the acyl acceptor,mediating the biosynthesis of galloylated macrocyclic polyphenolic compounds in P.chinense.These findings lay the foundation for elucidating the entire biosynthetic pathway of active polyphenols in this important herbal plant species.
基金supported by the National Key R&D Program of China,No.2021YFC2501200(to PC).
文摘Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’s disease.In this review,we summarize the changes in short-chain fatty acid levels and the abundance of short-chain fatty acid-producing bacteria in various samples from patients with Parkinson’s disease,highlighting the critical role of gut homeostasis imbalance in the pathogenesis and progression of the disease.Focusing on the nervous system,we discuss the molecular mechanisms by which short-chain fatty acids influence the homeostasis of both the enteric nervous system and the central nervous system.We identify key processes,including the activation of G protein-coupled receptors and the inhibition of histone deacetylases by short-chain fatty acids.Importantly,structural or functional disruptions in the enteric nervous system mediated by these fatty acids may lead to abnormalα-synuclein expression and gastrointestinal dysmotility,which could serve as an initiating event in Parkinson’s disease.Furthermore,we propose that short-chain fatty acids help establish communication between the enteric nervous system and the central nervous system via the vagal nerve,immune circulation,and endocrine signaling.This communication may shed light on their potential role in the transmission ofα-synuclein from the gut to the brain.Finally,we elucidate novel treatment strategies for Parkinson’s disease that target short-chain fatty acids and examine the challenges associated with translating short-chain fatty acid-based therapies into clinical practice.In conclusion,this review emphasizes the pivotal role of short-chain fatty acids in regulating gut-brain axis integrity and their significance in the pathogenesis of Parkinson’s disease from the perspective of the nervous system.Moreover,it highlights the potential value of short-chain fatty acids in early intervention for Parkinson’s disease.Future research into the molecular mechanisms of short-chain fatty acids and their synergistic interactions with other gut metabolites is likely to advance the clinical translation of innovative short-chain fatty acid-based therapies for Parkinson’s disease.
文摘Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(4,4'-dihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid),phen(1,10-phenanthroline),bpb(1,4-bis(pyrid-4-yl)benzene),bpa(bis(4-pyridyl)amine),and copper,nickel and cadmium chlorides at 160℃.The products were isolated as stable crystalline solids and were characterized by IR spectra,elemental analyses,thermogravimetric analyses,and singlecrystal X-ray diffraction analyses.Single-crystal X-ray diffraction analyses revealed that three complexes crystallize in the monoclinic P21/n,tetragonal I42d,and orthorhombic P21212 space groups.The complexes exhibit molecular dimers(1)or 2D metal-organic networks(2 and 3).The catalytic performances in the Knoevenagel reaction of these complexes were investigated.Complex 1 exhibits an effective catalytic activity and excellent reusability as a heterogeneous catalyst in the Knoevenagel reaction at room temperature.CCDC:2463800,1;2463801,2;2463802,3.
基金Supported by National Key Research&Development Program of China (2022YFB4201800)Key Program of National Natural Science Foundation of China (52130610)。
文摘Lignin-derived oxygenated aromatics,particularly phenols and aromatic ethers obtained through depolymerization,represent promising feedstocks for synthesizing high-density and high-heat-sink aviation fuels via alkylation-hydrogenation processes.This study systematically evaluates the catalytic performance of various zeolites(Hβ,HZSM-5,MCM-41 and HUSY)in the alkylation reaction of phenol with cyclohexanol.Characterization results demonstrate that HUSY zeolite showed superior catalytic activity compared to other zeolites,attributable to its favorable pore architecture and well-balanced acid site distribution that synergistically facilitate molecular diffusion and catalytic transformations.To further enhance the catalytic properties,HUSY zeolite was modified with citric acid at various concentrations and compared with those treated with NaOH and oxalic acid.The results revealed that citric acid treatment preserved the crystallinity of the zeolite while modulating its acid distribution and pore structure.All modified zeolites enhanced phenol alkylation activity.Notably,the HUSY-0.5M catalyst,which exhibited the highest medium-strong acid to total acid ratio,achieved superior catalytic performance,80.4%conversion of phenol and 99.6%selectivity for alkylation products.The catalyst also exhibited high activity in the alkylation of various lignin-derived compounds,demonstrating its broad applicability.This work provides a new strategy for the valorization of lignin-derived phenols into high-value fuel precursors through alkylation.
文摘Three zinc(Ⅱ),nickel(Ⅱ),and cadmium(Ⅱ)complexes,namely[Zn(μ-Htpta)(py)_(2)]n(1),[Ni(H_(2)biim)2(H_(2)O)2][Ni(tpta)(H_(2)biim)2(H_(2)O)]2·3H_(2)O(2),and[Cd_(3)(μ4-tpta)2(μ-dpe)_(3)]_(n)(3),have been constructed hydrothermally at 160℃ using H_(3)tpta([1,1':3',1″-terphenyl]-4,4',5'-tricarboxylic acid),py(pyridine),H_(2)biim(2,2'-biimidazole),dpe(1,2-di(4-pyridyl)ethylene),and zinc,nickel and cadmium chlorides,resulting in the formation of stable crystalline solids which were subsequently analyzed using infrared spectroscopy,element analysis,thermogravimetric analysis,as well as structural analyses conducted via single-crystal X-ray diffraction.The findings from these single-crystal Xray diffraction studies indicate that complexes 1-3 form crystals within the monoclinic system P2_(1)/c space group(1)or triclinic system P1 space group(2 and 3),and possess 1D,0D,and 3D structures,respectively.Complex 1 demonstrated substantial catalytic efficiency and excellent reusability as a heterogeneous catalyst in the reaction of Knoevenagel condensation under ambient temperature conditions.In addition,complex 1 also showcased notable anti-wear performance when used in polyalphaolefin synthetic lubricants.CCDC:2449810,1;2449811,2;2449812,3.
基金Supported by the Science and Technology Major Project of Liaoning Province(2024JH2,1025000892)the Fundamental Research Funds for the Universities of Liaoning Province(LJ232410143051)+1 种基金Liaoning Provincial Science and Technology Program 2023JHl/10400006Shenyang Science and Technology Program(24-213-3-09)。
文摘Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted acid site(BAS)strength on reaction pathways,alongside the spatial proximity effects between BAS and Mo active sites in bifunctional synergy,remains a critical scientific challenge in catalyst design.This study systematically tunes both BAS strength(via isomorphous metal substitution)and Mo-BAS spatial proximity in zeolites,integrating MDA catalytic evaluations with density functional theory(DFT)calculations to dissect their individual contributions.Strongly acidic BAS catalysts(compared to moderately acidic Fe/Ga-substituted counterparts)exhibit superior performance,evidenced by enhanced aromatic yields.Conversely,weakly acidic Bsubstituted zeolites demonstrate optimal mono-/bifunctional synergy,outperforming moderate-acid systems.DFT results reveal that acid strength dictates C−H activation mechanisms by modulating the energy barriers of rate-determining steps.While Al-zeolites deliver the highest activity,B-substituted systems display unique potential for mechanistic investigations.Spatial proximity analysis indicates that micrometer-scale Mo-BAS distances hinder effective synergy due to exceeding electron interaction and mass transfer limits,whereas nanometer-scale proximity enhances activity(via accelerated intermediate transport)and suppresses coke formation.These findings establish a theoretical framework for rationalizing zeolite catalyst optimization through BAS property engineering and spatial control of Mo-BAS cooperation,providing actionable guidelines for designing next-generation MDA catalysts.
文摘This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Comparative analysis of natural air(Air),controlled atmosphere(CA),and MAP treatments revealed that MAP upregulated the expression of the hercynylcysteine S-oxide synthase(HCSOS),aldehyde dehydrogenase(ALDH),and monoamine oxidase(MAO)genes,thereby enhancing histidine-derived ergothioneine and methionine levels,and subsequently boosting glutathione-mediated redox homeostasis.Meanwhile,MAP induced the expression of the dihydroxyacid dehydratase(DHAD),saccharopine dehydrogenase(SDH),and arginosuccinate lyase(ASL)genes,redirecting valine,lysine,and arginine into the tricarboxylic acid(TCA)cycle to fuel ATP production.MAP also enhanced ASL-mediated arginine degradation and urea cycle activity,reducing arginine accumulation when compared to CA treatment.In contrast,while MAP induced upregulated expression of the pyrroline-5-carboxylate dehydrogenase(P5CDH)and D-amino acid oxidase(DAAO)genes,CA treatment promoted proline accumulation,reflecting stress-specific metabolic flexibility.Collectively,these findings demonstrate that MAP triggers transcriptional reprogramming of amino acid metabolism to coordinate oxidative defense,energy generation,and osmotic balance.By modulating these metabolic pathways and regulatory genes under MAP conditions,fungal adaptability can be disrupted.Hence,this study provides a promising strategy for suppressing green mold development,extending the postharvest shelf life,and improving the quality of fruits and vegetables.
文摘Non-naturalα-amino acids(α-AAs)are pivotal in drug and catalysis,yet their synthesis remains challenges.A photocatalytic strategy for the direct construction of N-heteroaryl-containingα-AAs from heteroaryl aldehydes via acyl radical intermediates is reported.This method exhibits exceptional functional group tolerance and enables late-stage diversification of peptides and carbonyl-group derivatization.Mechanistic studies confirm a radical-based pathway,while applications in peptide modification highlight its utility in bioorganic chemistry.
基金Supported by National Natural Science Foundation(52374279)。
文摘The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-rich components of CGFS(CGFS-H)and systematically investigates the selective leaching behavior of Fe^(3+),Al^(3+)and Ca^(2+)using three organic acid extractants,i.e.,citric acid,tartaric acid,and tetrasodium iminodisuccinate.Additionally,the stepwise leaching of iron,aluminum and calcium from CGFS-H is explored.The selective dissolution mechanisms of these metals by different organic acids are elucidated through X-ray diffraction(XRD),X-ray fluorescence(XRF),and scanning electron microscopy(SEM)analyses.The results indicate that tetrasodium iminodisuccinate exhibits the highest leaching selectivity for Fe^(3+),while tartaric acid demonstrateds a comparable affinity for both Fe^(3+)and Al^(3+).In contrast citric acid shows superior selectivity toward Ca^(2+).The leaching yield of Fe^(3+),Al^(3+)and Ca^(2+)after sequential leaching with the three organic acids were 79.8%,65.08%and 78.6%,respectively.These findings confirm that effective and selective separation of Fe^(3+),Al^(3+)and Ca^(2+)from CGFS-H can be achieved via optimized organic acid-based leaching strategies.This advancement provides a critical foundation for developing Ca/Fe/Al hydrotalcite materials using CGFS-H as a sustainable feedstock,thereby facilitating the transformation of waste residue into high-value functional materials and promoting resourceefficient utilization of coal gasification fine slag.
文摘Under hydrothermal and solvothermal conditions,two novel cobalt-based complexes,{[Co_(2)(CIA)(OH)(1,4-dtb)]·3.2H_(2)O}n(HU23)and{[Co_(2)(CIA)(OH)(1,4-dib)]·3.5H2O·DMF}n(HU24),were successfully constructed by coordinatively assembling the semi-rigid multidentate ligand 5-(1-carboxyethoxy)isophthalic acid(H₃CIA)with the Nheterocyclic ligands 1,4-di(4H-1,2,4-triazol-4-yl)benzene(1,4-dtb)and 1,4-di(1H-imidazol-1-yl)benzene(1,4-dib),respectively,around Co^(2+)ions.Single-crystal X-ray diffraction analysis revealed that in both complexes HU23 and HU24,the CIA^(3-)anions adopt aκ^(7)-coordination mode,bridging six Co^(2+)ions via their five carboxylate oxygen atoms and one ether oxygen atom.This linkage forms tetranuclear[Co4(μ3-OH)2]^(6+)units.These Co-oxo cluster units were interconnected by CIA^(3-)anions to assemble into 2D kgd-type structures featuring a 3,6-connected topology.The 2D layers were further connected by 1,4-dtb and 1,4-dib,resulting in 3D pillar-layered frameworks for HU23 and HU24.Notably,despite the similar configurations of 1,4-dtb and 1,4-dib,differences in their coordination spatial orientations lead to topological divergence in the 3D frameworks of HU23 and HU24.Topological analysis indicates that the frameworks of HU23 and HU24 can be simplified into a 3,10-connected net(point symbol:(4^(10).6^(3).8^(2))(4^(3))_(2))and a 3,8-connected tfz-d net(point symbol:(4^(3))_(2)((4^(6).6^(18).8^(4)))),respectively.This structural differentiation confirms the precise regulatory role of ligands on the topology of metal-organic frameworks.Moreover,the ultraviolet-visible absorption spectra confirmed that HU23 and HU24 have strong absorption capabilities for ultraviolet and visible light.According to the Kubelka-Munk method,their bandwidths were 2.15 and 2.08 eV,respectively,which are consistent with those of typical semiconductor materials.Variable-temperature magnetic susceptibility measurements(2-300 K)revealed significant antiferromagnetic coupling in both complexes,with their effective magnetic moments decreasing markedly as the temperature lowered.CCDC:2457554,HU23;2457553,HU24.
文摘5,5'-dithiobis(2-nitrobenzoic acid)(H_(2)DTNB)was employed as the second ligand to react with cucurbit[6]uril(Q[6])and Cd(NO_(3))_(2),and it was deprotonated or transformed into HDTNB^(-),TNB^(2-)and NSB^(2-)(H_(2)TNB=5,5'-thiobis(2-nitrobenzoic acid),H_(2)NSB=2-nitro-5-sulfobenzoic acid)under different conditions to afford three novel supramolecular assemblies with the formulas of[Cd(H_(2)O)_(4)(Q[6])](HDTNB)_(2)·3H_(2)O(1),[Cd(H_(2)O)_(6)]_(2)(TNB)_(2)·Q[6]·4H_(2)O(2)and[Cd(H_(2)O)_(5)(NSB)]_(2)·Q[6](3).Singe-crystal diffraction(SC-XRD)analysis revealed that assembly 1 is constructed from 2D[Cd(H_(2)O)_(4)(Q[6])]2+supramolecular layers and HDTNB^(-)supra molecular layers,the structure of assembly 2 is comprised of the 2D{[Cd(H_(2)O)_(6)]_(2)·Q[6]}^(4+)supramolecular layers and 1D TNB^(2-)supramolecular chains,while assembly 3 is built from the 3D Q[6]frameworks with[Cd(H_(2)O)_(5)(NSB)]supramolecular chains filled in the pores.Meanwhile,the noncovalent interactions between the ligands HDTNB^(-)/TNB^(2-)/NSB^(2-)and the outer-surface of Q[6]molecules contributed greatly to the formation of the supramolecular architecture of assemblies 1-3.CCDC:2522253,1;2522254,2;2522255,3.
文摘The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.The complex was characterized by IR,^(1)H(^(13)C)NMR,elemental analysis,and powder X-ray diffraction,and the crystal structure was determined by single-crystal X-ray diffraction.The crystal belongs to the orthorhombic system with space group Iba2,and the central tin atom is in a five-coordinated trigonal bipyramidal configuration.Quantum chemistry ab initio calculations were performed to investigate the stability,molecular orbital energy,and frontier molecular orbital characteristics of the complex.Additionally,its thermal stability,electrochemical properties,and in vitro anticancer activity were evaluated.CCDC:2380308.
文摘In this study,polyacrylic acid(PAA)films were employed as a model system,and a series of PAA films with tunable water wettability was systematically prepared by varying molecular weight and curing temperature.Using attenuated total reflectance Fourier-transform infrared spectroscopy(ATR-FTIR),the molecular configurations of surface carboxyl groups(COOH),free carboxyl(COOH_(f))and hydrogen-bonded carboxyl(COOH_(HB),were directly correlated with the polar component of surface energy(γ^(s,p)).By decomposing theγ^(s,p)values of the PAA thin films as a sum of the contributions of COOH_(f)and COOH_(H B),the intrinsic polar component of surface energy of COOH_(H B)(γ_(H B)^(s,p*))was quantified for the first time as 8.34 mN/m,significantly lower than that of COOH_(f)(γ_(f)^(s,p*)=34 mN/m).This result highlights that hydrogen bonding markedly reduces theγ^(s,p),providing a rational explanation for the relatively large water contact angle observed on PAA thin films.Furthermore,it establishes a thermodynamic basis for estimating the fraction of surface COOH_(H B)groups(f H B)from wettability measurements.Further extension of the model to carboxyl-terminated self-assembled monolayers(COOH-SAMs)revealed that surface COOH density(ΣCOOH)critically regulates wetting behavior:whenΣCOOH ranges from 4.30 to 5.25 nm^(-2),COOH groups predominantly exist in a free state and facilitate effective hydration layers,thereby promoting superhydrophilicity.Overall,this study not only establishes a unified thermodynamic framework linking surface COOH configurations to macroscopic wettability,but also validates its universality by extending it to COOH-SAMs systems,thereby providing a unified theoretical framework for the controllable design of hydrophilicity in various COOH-functionalized surfaces.
文摘BACKGROUND:Breast hyperplasia is a common benign breast disease mainly caused by endocrine disorders,manifested as abnormal hyperplasia of breast tissue.In recent years,traditional Chinese medicine compounds and probiotics have shown good potential in regulating the endocrine system and improving the intestinal microecology,providing new ideas for the treatment of breast hyperplasia.OBJECTIVE:To explore the effects and mechanisms of traditional Chinese medicine compounds and fermented probiotic compounds on breast hyperplasia in mice,providing new theoretical and experimental bases for the clinical treatment and prevention of breast hyperplasia.METHODS:(1)Network pharmacology tools were used to predict the anti-breast-hyperplasia activity of Herba Gueldenstaedtiae(Euphorbia humifusa),as well as its potential targets and signaling pathways.The databases included:TCMSP,OMIM,GeneCards database,UniProt website,Venny2.1.0 website,Metascape,HERB website,and STRING database,all of which are open-access databases.Network pharmacology can predict and screen key information such as the targets corresponding to the active ingredients of traditional Chinese medicine,disease targets,and action pathways through network analysis and computer-system analysis.Therefore,it has been increasingly widely used in the research of traditional Chinese medicine.(2)A breast hyperplasia model was induced in mice by injecting estrogen and progesterone.Mice in the normal blank group were injected intraperitoneally with normal saline every day.Mice in the model group and drugadministration groups were injected intraperitoneally with estradiol benzoate injection at a concentration of 0.5 mg/kg every day for 25 days.From the 26th day,the injection of estradiol benzoate injection was stopped.Mice in the normal blank group were injected intramuscularly with normal saline every day,and mice in the model group and drug-administration groups were injected intramuscularly with progesterone injection at a concentration of 5 mg/kg for 5 days.After the model was established,each group was given drugs respectively.The normal blank group and the model group were gavaged with 0.2 mL/d of normal saline;the positive blank group(Xiaozheng Pill group)was gavaged with an aqueous solution of Xiaozheng Pill at 0.9 mg/g;the low-,medium-and high-dose groups of Compound Herba Gueldenstaedtiae were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively;the low-,medium-and high-dose groups of traditional Chinese medicine-bacteria fermentation were gavaged with an aqueous solution of the compound medicine at 0.75,1.5,and 3.0 mg/(g·d)respectively.The administration was continuous for 30 days.RESULTS AND CONCLUSION:(1)The results of network pharmacology research showed that the Compound Herba Gueldenstaedtiae(Euphorbia humifusa)contained 46 active ingredients,which were related to 1213 potential targets.After comparison with 588 known breast-hyperplasia targets,it was speculated that 50 of these targets might be related to the direct effect of the compound on breast hyperplasia.(2)After drug intervention,there was no significant change in the high-dose group of Compound Herba Gueldenstaedtiae compared with the normal blank group.The liver indicators of the other intervention groups all significantly decreased(P<0.05).(3)In terms of kidney and uterine indicators,the medium-dose group of Compound Herba Gueldenstaedtiae decreased significantly compared with the normal blank group(P<0.05).In terms of the uterine index,the model group increased significantly compared with the normal blank group(P<0.01).(4)After 1-month drug treatment,the number of lobules and acini in the breast tissue of the Xiaozheng Pill group,the low,medium,and high-dose group of Compound Herba Gueldenstaedtiae,the low,medium,and highdose groups of traditional Chinese medicine-bacteria fermentation decreased,and the duct openings narrowed.With the increase of drug dose,diffuse hyperplasia of breast tissue was significantly improved.(5)The ELISA results showed that compared with the model group,the estrogen level was lower in the medium-dose group of traditional Chinese medicine-bacteria fermentation after the intervention(P<0.05).In addition,the follicle-stimulating hormone level in the low-dose group of Compound Herba Gueldenstaedtiae was lower than that of the model group(P<0.05).(6)The intervention in the mouse model led to changes in the abundance of short chain fatty acids and intestinal flora in all groups.To conclude,the Compound Herba Gueldenstaedtiae and its probiotic fermentation products significantly improved mammary gland hyperplasia in mice by regulating hormone levels,improving the structure of the gut microbiota,and increasing the content of shortchain fatty acids,providing new ideas and potential sources of drugs for the treatment of breast hyperplasia.
