This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici str...This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.展开更多
Two lactic acid bacteria strains (At1BEAE22 and At344E21) isolated during tchapalo production were identified on the basis of phenotypic analyses. Bacteriocins produced by these strains were tested for their antimicro...Two lactic acid bacteria strains (At1BEAE22 and At344E21) isolated during tchapalo production were identified on the basis of phenotypic analyses. Bacteriocins produced by these strains were tested for their antimicrobial activities using well diffusion agar method. Heat resistance, pH sensitivity and enzyme treatments were also analyzed. Results showed that both lactic acid bacteria strains were identified as Pediococcus acidilactici. Their bacteriocins inhibited growth of Lactobacillus delbrueckii F/31, Listeria innocua ATCC 33090, Enterococcus faecalis, Enterococcus faecalis ATCC 29212, Streptococcus sp, Enterococcus faecalis CIP 105042 and Enterococcus faecium ATCC 51558. These bacteriocins were heat stable at 60°C for 30 min for all indicator bacteria. However, they remained active only against Lactobacillus delbrueckii and Listeria innocua at 121°C for 60 min. Moreover, they were active in a wide range of pH (3 to 9) with a maximum activity observed at pH 5 and 6 on all indicator bacteria. But, bacteriocin from Pediococcus acidilactici At34E21 was more stable at acidic pH than basic one. The fact that the bacteriocin was inactivated by proteinase K and α-chymotrypsin indicated its proteinaceous nature, a general characteristics of bacteriocins.展开更多
Exopolysaccharides(EPS)are widely used in the food,pharmaceutical,biomaterial and other fields.Lactic acid bacteria(LAB),which are generally regarded as safe,are among the main microbial sources of EPS.In this study,a...Exopolysaccharides(EPS)are widely used in the food,pharmaceutical,biomaterial and other fields.Lactic acid bacteria(LAB),which are generally regarded as safe,are among the main microbial sources of EPS.In this study,an EPS-producing strain,designated Pediococcus acidilactici J1,was isolated and purified from a fruit fermentation broth and identified based on its morphology,API 50 CHL carbohydrate fermentation profile,16S rDNA sequence analysis,and the partial physicochemical properties of the EPS produced by strain J1(J1 EPS).The colonies of strain J1 are round,grayish-white,with smooth surfaces and neatly defined edges.A 1434 bp 16S rDNA gene sequence is obtained and shows the highest similarity to Pediococcus acidilactici 3-161 in homology analysis.The EPS yield of J1 reaches a maximum of 18.63 g·L^(-1) at 36 h,with a production rate of 0.52 g·L^(-1)·h^(-1),which is slightly higher than that reported for other strains.In addition,J1 EPS exhibits high water solubility of(98.19±1.62)%,water-holding capacity of(406.52±6.55)%,surface hydrophobicity,good thermal stability and strong emulsifying activity.These results provide a basis for the potential application of Pediococcus acidilactici J1 EPS as a functional biopolymer in the food industry and related fields.展开更多
Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the undere...Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the underexplored medicinal potential of DF,we developed an efficient fermentation method to enhance the bioavailability of okara fiber.In this study,Pediococcus acidilactici IFJ-1,which has strong enzymatic production capabilities and beneficial effects on gastrointestinal flora modulation,was selected to ferment okara.Results showed decreases in viscosity and particle size,optimized surface structure,improved thermal stability and hydration properties,and a significant increase in soluble DF content from 1.85%to 3.91%.To evaluate the physiological effects,hyperlipidemic mouse models were established and subjected to dietary interventions utilizing okara and fermented okara to measure changes in physicochemical parameters,gut microbiota composition,and lipid metabolism.The dietary intervention was effective,particularly in the fermented okara group,showing a 7.3%weight loss,improved blood lipids(triglycerides:‒39.8%,total cholesterol:‒12.8%,low-density lipoprotein cholesterol:‒34.2%,high-density lipoprotein cholesterol:+26.2%),and a 22.2%lower liver index.Gut microbiota analysis revealed that fermented okara positively modulated the microbial community by increasing the abundance of beneficial bacteria(e.g.,Bacteroidota)and reducing the abundance of obesity-associated bacteria(e.g.,Bacillota).Lipid metabolism profiling further demonstrated that fermented okara downregulated harmful lipids(e.g.,(O-acyl)-ω-hydroxy fatty acids,ceramides,and diacylglycerols)while upregulating beneficial phospholipids(e.g.,phosphatidylinositol,phosphatidylserine,phosphatidylethanolamine,lysophosphatidylinositol and lysophosphatidic acid).This study highlights a novel approach for enhancing DF utilization through fermentation,providing valuable insights into strategies for preventing obesity and metabolic diseases.展开更多
本研究以不同浓度的乳清分离蛋白(Whey Protein Isolate,WPI)、果胶(Pectin,PE)以及海藻酸钠(Alginate,ALG)作为原料,通过凝胶化法包封乳酸片球菌(Pediococcus acidilactici,PA),制备了3种微胶囊。通过对不同壁材制备的微胶囊进行形态...本研究以不同浓度的乳清分离蛋白(Whey Protein Isolate,WPI)、果胶(Pectin,PE)以及海藻酸钠(Alginate,ALG)作为原料,通过凝胶化法包封乳酸片球菌(Pediococcus acidilactici,PA),制备了3种微胶囊。通过对不同壁材制备的微胶囊进行形态表征、乳酸片球菌的包封效率以及模拟消化后乳酸片球菌的存活率进行分析,研究微胶囊壁材的最佳配比。此外,通过测定不同温度和时间条件下储藏后乳酸片球菌的存活率,评估微胶囊的贮藏稳定性。结果表明,ALG-WPI-PE-PA微胶囊在冻干后保持了均匀的球形外观,形态优于ALGPA和ALG-WPI-PA微胶囊。当WPI浓度为3.0%时,微胶囊的包封效率显著提高(P<0.05),其中ALG-WPIPA的包封效率为71.93%,而ALG-WPI-PE-PA的包封效率达到94.33%,提高了22.4%。此外,经过模拟体外消化后,包封的乳酸片球菌存活率显著提高(P<0.05)。研究确定了WPI在微胶囊中的最佳浓度为3.0%,在该浓度下,乳酸片球菌的包封效率和消化后的存活率均达到最佳水平。通过贮藏实验,进一步验证了ALG-3.0%WPI-PEPA能够显著提高乳酸片球菌在长时间储存条件下的生存力。本研究为乳酸菌包封技术的进一步开发和应用提供了基础,并为益生菌制剂产品的多样化提供了新的思路。展开更多
基金supported by the National Natural Science Foundation of China Program(32021005)the Natural Science Foundation of Jiangsu Province(BK20200084)+2 种基金Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps(2018DB002)National First Class Discipline Program of Food Science and Technology(JUFSTR20180102)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.
文摘Two lactic acid bacteria strains (At1BEAE22 and At344E21) isolated during tchapalo production were identified on the basis of phenotypic analyses. Bacteriocins produced by these strains were tested for their antimicrobial activities using well diffusion agar method. Heat resistance, pH sensitivity and enzyme treatments were also analyzed. Results showed that both lactic acid bacteria strains were identified as Pediococcus acidilactici. Their bacteriocins inhibited growth of Lactobacillus delbrueckii F/31, Listeria innocua ATCC 33090, Enterococcus faecalis, Enterococcus faecalis ATCC 29212, Streptococcus sp, Enterococcus faecalis CIP 105042 and Enterococcus faecium ATCC 51558. These bacteriocins were heat stable at 60°C for 30 min for all indicator bacteria. However, they remained active only against Lactobacillus delbrueckii and Listeria innocua at 121°C for 60 min. Moreover, they were active in a wide range of pH (3 to 9) with a maximum activity observed at pH 5 and 6 on all indicator bacteria. But, bacteriocin from Pediococcus acidilactici At34E21 was more stable at acidic pH than basic one. The fact that the bacteriocin was inactivated by proteinase K and α-chymotrypsin indicated its proteinaceous nature, a general characteristics of bacteriocins.
基金Supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2015BAD16B01)Tianjin Key Technology Research and Development Support Program (13ZCDNC01900)
基金Supported by the National Natural Science Foundation of China(32072189)the Heilongjiang Province Provincial Colleges and Universities Basic Scientific Research Business Expenses Scientific Rsearch Projects(2024-KYYWF-0127)。
文摘Exopolysaccharides(EPS)are widely used in the food,pharmaceutical,biomaterial and other fields.Lactic acid bacteria(LAB),which are generally regarded as safe,are among the main microbial sources of EPS.In this study,an EPS-producing strain,designated Pediococcus acidilactici J1,was isolated and purified from a fruit fermentation broth and identified based on its morphology,API 50 CHL carbohydrate fermentation profile,16S rDNA sequence analysis,and the partial physicochemical properties of the EPS produced by strain J1(J1 EPS).The colonies of strain J1 are round,grayish-white,with smooth surfaces and neatly defined edges.A 1434 bp 16S rDNA gene sequence is obtained and shows the highest similarity to Pediococcus acidilactici 3-161 in homology analysis.The EPS yield of J1 reaches a maximum of 18.63 g·L^(-1) at 36 h,with a production rate of 0.52 g·L^(-1)·h^(-1),which is slightly higher than that reported for other strains.In addition,J1 EPS exhibits high water solubility of(98.19±1.62)%,water-holding capacity of(406.52±6.55)%,surface hydrophobicity,good thermal stability and strong emulsifying activity.These results provide a basis for the potential application of Pediococcus acidilactici J1 EPS as a functional biopolymer in the food industry and related fields.
基金supported by the Key Special Projects of the Ministry of Science and Technology(SQ2020YFF0404523)the North Anhui Soybean Advantageous Characteristic Industry Cluster Project(2023CYJQ013)+2 种基金the National Natural Science Foundation of China(32172162)the Key Genetic Technologies Research and Development Program of Hefei(2021GJ075)the Young Talents Program of Anhui Academy of Agricultural Science(QNYC-202122).
文摘Okara is produced in large quantities annually in China,but much of it is discarded due to its high content of indigestible dietary fiber(DF),contributing to significant environmental challenges.Recognizing the underexplored medicinal potential of DF,we developed an efficient fermentation method to enhance the bioavailability of okara fiber.In this study,Pediococcus acidilactici IFJ-1,which has strong enzymatic production capabilities and beneficial effects on gastrointestinal flora modulation,was selected to ferment okara.Results showed decreases in viscosity and particle size,optimized surface structure,improved thermal stability and hydration properties,and a significant increase in soluble DF content from 1.85%to 3.91%.To evaluate the physiological effects,hyperlipidemic mouse models were established and subjected to dietary interventions utilizing okara and fermented okara to measure changes in physicochemical parameters,gut microbiota composition,and lipid metabolism.The dietary intervention was effective,particularly in the fermented okara group,showing a 7.3%weight loss,improved blood lipids(triglycerides:‒39.8%,total cholesterol:‒12.8%,low-density lipoprotein cholesterol:‒34.2%,high-density lipoprotein cholesterol:+26.2%),and a 22.2%lower liver index.Gut microbiota analysis revealed that fermented okara positively modulated the microbial community by increasing the abundance of beneficial bacteria(e.g.,Bacteroidota)and reducing the abundance of obesity-associated bacteria(e.g.,Bacillota).Lipid metabolism profiling further demonstrated that fermented okara downregulated harmful lipids(e.g.,(O-acyl)-ω-hydroxy fatty acids,ceramides,and diacylglycerols)while upregulating beneficial phospholipids(e.g.,phosphatidylinositol,phosphatidylserine,phosphatidylethanolamine,lysophosphatidylinositol and lysophosphatidic acid).This study highlights a novel approach for enhancing DF utilization through fermentation,providing valuable insights into strategies for preventing obesity and metabolic diseases.
文摘本研究以不同浓度的乳清分离蛋白(Whey Protein Isolate,WPI)、果胶(Pectin,PE)以及海藻酸钠(Alginate,ALG)作为原料,通过凝胶化法包封乳酸片球菌(Pediococcus acidilactici,PA),制备了3种微胶囊。通过对不同壁材制备的微胶囊进行形态表征、乳酸片球菌的包封效率以及模拟消化后乳酸片球菌的存活率进行分析,研究微胶囊壁材的最佳配比。此外,通过测定不同温度和时间条件下储藏后乳酸片球菌的存活率,评估微胶囊的贮藏稳定性。结果表明,ALG-WPI-PE-PA微胶囊在冻干后保持了均匀的球形外观,形态优于ALGPA和ALG-WPI-PA微胶囊。当WPI浓度为3.0%时,微胶囊的包封效率显著提高(P<0.05),其中ALG-WPIPA的包封效率为71.93%,而ALG-WPI-PE-PA的包封效率达到94.33%,提高了22.4%。此外,经过模拟体外消化后,包封的乳酸片球菌存活率显著提高(P<0.05)。研究确定了WPI在微胶囊中的最佳浓度为3.0%,在该浓度下,乳酸片球菌的包封效率和消化后的存活率均达到最佳水平。通过贮藏实验,进一步验证了ALG-3.0%WPI-PEPA能够显著提高乳酸片球菌在长时间储存条件下的生存力。本研究为乳酸菌包封技术的进一步开发和应用提供了基础,并为益生菌制剂产品的多样化提供了新的思路。
