Ischemia-reperfusion(I/R)injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure.Antagonizing inflammatory responses and oxidative stress are regarded as crucial...Ischemia-reperfusion(I/R)injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure.Antagonizing inflammatory responses and oxidative stress are regarded as crucial targets for mitigating reperfusion injury and enhancing flap survival.In this study,caffeic acid-vanadium metal polyphenol nanoparticles(CA-V NPs)were prepared for the treatment of skin flap ischemia and reperfusion.This study was conducted using a one-step method to prepare new types of CA-V NPs with uniform sizes and stable structures.In vitro,the CA-V NPs exhibited CATlike and SOD-like activities and could effectively scavenge ROS,generate oxygen,and alleviate oxidative stress.In the H_(2)O_(2)-induced cellular oxidative stress model,CA-V NPs effectively reduced ROS levels and inhibited apoptosis through the XIAP/Caspase-3 pathway.In the cellular inflammation model induced by LPS combined with IFN-g,CA-V NPs reprogrammed macrophage polarization toward the M2 phenotype and reduced inflammatory responses by reducing the expression of the chemokines CCL4 and CXCL2.In addition,animal experiments have shown that CA-V NPs can alleviate oxidative stress in skin flap tissues,inhibit apoptosis,promote angiogenesis,and ultimately improve the survival rate of skin flaps.CA-V NPs provide a new target and strategy for the treatment of flap I/R injury。展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.82172204,82372552 and 82372517)the Anhui Key Research and Development Plan(Grant No.202104j07020027,China)+3 种基金the Excellent Youth of Natural Science Research Projects in Anhui Province Universities(2023AH030060)the Basic and Clinical Cooperative Research and Promotion Program of Anhui Medical University(No.2021xkjT028,China)the Research Fund of Anhui Institute of Translational Medicine(2022zhyx-C01,China)the Scientific Research Programme for Higher Education Institutions in Anhui Province(2023AH040371,China).
文摘Ischemia-reperfusion(I/R)injury following skin flap transplantation is a critical factor leading to flap necrosis and transplant failure.Antagonizing inflammatory responses and oxidative stress are regarded as crucial targets for mitigating reperfusion injury and enhancing flap survival.In this study,caffeic acid-vanadium metal polyphenol nanoparticles(CA-V NPs)were prepared for the treatment of skin flap ischemia and reperfusion.This study was conducted using a one-step method to prepare new types of CA-V NPs with uniform sizes and stable structures.In vitro,the CA-V NPs exhibited CATlike and SOD-like activities and could effectively scavenge ROS,generate oxygen,and alleviate oxidative stress.In the H_(2)O_(2)-induced cellular oxidative stress model,CA-V NPs effectively reduced ROS levels and inhibited apoptosis through the XIAP/Caspase-3 pathway.In the cellular inflammation model induced by LPS combined with IFN-g,CA-V NPs reprogrammed macrophage polarization toward the M2 phenotype and reduced inflammatory responses by reducing the expression of the chemokines CCL4 and CXCL2.In addition,animal experiments have shown that CA-V NPs can alleviate oxidative stress in skin flap tissues,inhibit apoptosis,promote angiogenesis,and ultimately improve the survival rate of skin flaps.CA-V NPs provide a new target and strategy for the treatment of flap I/R injury。
